Multiplex PCR Detection for 3 Events of Genetically Modified Maize, DAS-59122-7, TC6275, and MIR604

Ji-Hye Ahn,Jae-Hwan Kim,Su-Youn Kim,Woo-Young Lee,Sun-Hee Park,Hae-Yeong Kim 한국식품과학회 2008 Food Science and Biotechnology Vol.17 No.3

A multiplex polymerase chain reaction (PCR) method was developed to simultaneously detect 3 events of genetically modified (GM) maize. The event-specific primers were used to discriminate the following 3 events of GM maize (DAS-59122-7, TC6275, and MIR604) using multiplex PCR method. The zein gene was used as an endogenous maize reference gene in the multiplex PCR detection. The primer pair Zein-F/R producing a 99 bp amplicon was used to amplify the zein gene. The primer JI-Das-F1/R1 for DAS-59122-7, JI-TC6275-F3/R3 for TC6275, and JI-MIR F1/R1 for MIR604 yielded an amplicon of 130, 162, and 197 bp, respectively. The detection limit of multiplex PCR was 1% for DAS-59122-7, TC6275, and MIR604 for one reaction.

자유연애의 이상과 식민지 근대의 현실 : 김동인의 「약한 자의 슬픔」 연구

김지영 우리어문학회 2003 우리어문연구 Vol.20 No.-

The acclamation for free marriage and free love was an important constituent of the process of attempting to construct the institutional emotion and family structure newly modeling the modern western romantic love. Until the late 1910s, the idea of free marriage and free love was too strongly tied with the ideology of enlightenment, thus only part of the meaning, the freedom to choose a marital partner, was stressed. Moreover, marriage was strongly tied up with the will to devote toward the nation, so choosing a marital partner was on the will to be a good people for the nation rather than on natural feeling of love. Free love started to be described in its genuine meaning from the works of Kim Tongin and his colleagues of the literary magazine "Creation", though the meaning of it was still unstable and on the way of changing. "The Sorrow of the Weak" showed the inauthenticity of the concept of free love by describing a woman's inner inconsistence and the ordeals that happened in her life while she aspired to romantic love. Kim, the author, showed the leftovers of pre-modern ethic inside a woman of the time who was seeking modern life and revealed the abstractness of her admiration for romantic love. We can find the contradiction between pre-modern and modern love ethics and the conflict between physical and spiritual love in the protagonist's interior. The falsehood, disorganization, and inversion of love revealed by Elizabeth while she was chasing after romantic love and modern life were not different from the falsehood, disorganization, and inversion of the idea of free love of the period itself. This reflects one of the colonial characteristics of Korean modernization that was considered as the most urgent and transcendental aim by many Korean enlighteners of the day.

영상융합방법에 의한 거리측정

김지영,이희만 서원대학교 응용과학연구소 2000 응용과학연구 Vol.9 No.1

A stereo fusion method for measuring the distance from the multiple cameras to the target is proposed. the Parallel Looking Camera Model is employed and analyzed in this paper. The peak-valley shape feature is extracted from the gray level waveform on the epipolar line of the image plane. The matching process uses not only the said peak-valley information but also the gray level intensity of the local peak-valley waveform on the candidate waveforms. The left image is used as a reference for registering the right image. The right image is warpped for the said registration. The fused image which is a overlapped image of the reference left and the warpped right image is used to calculate RMSE(Root Mean Square Error). The fusion process is ended when the calculated RMSE value reaches its minimum level or the bottom threshold. The distance is obtained during the warping process by using the Parallel Looking Camera Model parameters.

영상처리에 의한 버스전용차로 위반차량의 검출방법

김지영 서원대학교 응용과학연구소 2001 응용과학연구 Vol.10 No.1

In this paper, an image processing method detecting the vehicles which violate the exclusive bus lain traffic regulation is proposed. The tow cameras are used to capture the images of the first lain of a highway where the exclusive bus lain traffic regulation is enforced. One camera is used to capture the front view of a vehicle, and the second camera to capture the side view of the craft. The front view camera is used for recognizing the vehicle licence number. The side view camera is employed for categorizing the vehicle class. The proposed method in this paper is very practical to be implemented because it does not need any attachment on the vehicle such as an identification transmitter or any other electronic devices.

전산전문 교육의 현황 및 추세

김지영 서원대학교 미래창조연구소 2004 과학과 문화 Vol.1 No.1

Since the advent of computers to current computer ubiquity, the efforts to educate the computer specialists to serve the needs of our society and the field, have been ever changing and expanding. This article traces the trend of computer education, more broadly, information technology education in general and at the college level. The progress of computer development is divided into several stages and the corresponding adaptation of the computer education is presented: From the time that computer not as an independent academic field, to an era that almost all disciplines are pervasively related to computers in one way or another.

車體構造解析硏究

김지홍,김천욱,이신영 연세대학교 산업기술연구소 1988 논문집 Vol.20 No.1

An analysis on the load-bearing capacity of floor frame and body structure is investigated by the three dimensional rigid frame analysis. The load-bearing capacity of the body structure is estimated on the basis of comparison to the floor-frame-only structure. The location and magnitude of the maximum displacement are also determined and show good agreement with those of a previous study.In the present study the load-bearing capacity of the floor frame of van-type structures is about 80 percent of the total loadings.

신경인성 방광 1례에 대한 임상고찰

김만호,이지영,이정원,조충식,김철중 대전대학교 한방병원 2002 惠和醫學 Vol.11 No.1

From January 10th 2002 to February 17th 2002, the clinical study was carried out I case of patient in trouble with voiding difficulty such as urinary incontinence, enuresis and urinary frequency after the L₄-L_(5) HN P operation, who had been hospitalised in the Dept. of Internal Medicin e, Cheon-An Oriental Medical Hospital, Daejeon University. The results were as following 1. For this case, we could get the better result using Bo-Joong-lk-Gi-Tang(補中益氣湯) in the treating period. 2. We could get the effective result that the case of voiding difficulty after HNP operation and cerebral infarction by conservative therapy of oriental medicine such as acupuncture, moxibustion and herb medicine.

GC-ECD를 이용한 유기염소계 및 Pyrethroid계 농약 동시 분석법

김우성,이선화,김재이,정지윤,이명자,박영채,이영자,정성욱,이봉헌,박흥재 한국환경과학회 2003 한국환경과학회지 Vol.12 No.4

Pesticides were extracted from samples with 70% acetone and methylene chloride in order, and then cleaned up via open-column chromatography apparatus packed with florisil, and finally analyzed simultaneously the organochlorine and pyrethroid pesticides using GC(ECD). An ultra-2 fused silica capillary column was used to separate and identify the products. The resolution between the last isomeric peak of cypermethrin(59.987min) and the first isomeric peak of flucythrinate(60.043min) was not satisfactory. The last isomeric peak of fenvalerate(62.344min) and the first isomeric peak of fluvalinate(62.397min) were overlapped. Recoveries of soybean sample for the most pesticides were 73.3% to 102.4%. Detection limits were between 0.004 and 0.063 ㎍/㎎ when this method was used.

남녀 고등학생의 교복과 자율복의 대한 태도 연구

김미정,김영숙,배지혜,신영옥,장명희,황인순 효성여자대학교 가정대학 학도호국단 1984 家政大論集 Vol.3 No.-

The purpose of this study was to investigate the attitude difference toward the school uniform, it was supposed that clothing acceptance was differentiated according to subjects. The subjects were grouped by 3 types which were students, teachers(school governors) and parents groups. The results were as follows; 1. Boy students reguired the more freedom of clothing than girls. 2. Teachers and parents groups considered that clothing affected student's behavior and self-esteem. 3. The acceptance of school plain dress was affected by atomosphere of home and school. 4. According to socioeconomic levels, there were differences in clothing acceptance and fashion acceptance.

텔레매틱스용 광대역 LNA 설계 및 제작

김지윤,양두영 제주대학교 공과대학 첨단기술연구소 2005 尖端技術硏究所論文集 Vol.16 No.2

In this paper, the wideband low noise amplifier for telematics terminals is proposed. In order to stabilize the input and output VSWR and minimize the noise level over wide frequency ranges, the balanced configuration and double open-stub matching circuit are adaptive. And to obtain high gain over 30dB, two amplifiers composed of MGA-86563 PHEMT are connected in cascade. From the results, the proposed low noise amplifier has flat gain over 0.8-2.6GHz, low VSWR and minimum noise figure in the desired bandwidth. Total gain of LNA is 35dB and noise figure and VSWR are 2.5dB and 1.3, respectively.