BMB Reports : Connexin32 inhibits gastric carcinogenesis through cell cycle arrest and altered expression of p21^Cip1 and p27^Kip1

( Hyang Jee ),( Su Hyung Lee ),( Jun Won Park ),( Bo Ram Lee ),( Ki Taek Nam ),( Dae Yong Kim ) 생화학분자생물학회(구 한국생화학분자생물학회) 2013 BMB Reports Vol.46 No.1

Gap junctions and their structural proteins, connexins (Cxs), have been implicated in carcinogenesis. To explore the involvement of Cx32 in gastric carcinogenesis, immunochemical analysis of Cx32 and proliferation marker Ki67 using tissue-microarrayed human gastric cancer and normal tissues was performed. In addition, after Cx32 overexpression in the human gastric cancer cell line AGS, cell proliferation, cell cycle analyses, and p21^Cip1 and p27^Kip1 expression levels were examined by bromodeoxyuridine assay, flow cytometry, real-time RT-PCR, and western blotting. Immunohistochemical study noted a strong inverse correlation between Cx32 and Ki67 expression pattern as well as their location. In vitro, overexpression of Cx32 in AGS cells inhibited cell proliferation significantly. G1 arrest, up-regulation of cell cycle-regulatory proteins p21^Cip1 and p27^Kip1 was also found at both mRNA and protein levels. Taken together, Cx32 plays some roles in gastric cancer development by inhibiting gastric cancer cell proliferation through cell cycle arrest and cell cycle regulatory proteins. [BMB Reports 2013; 46(1): 25-30]

표준압밀시험과 일정변형률압밀시험에 의한 연약점성토의 압밀계수 추정에 관한 연구

이기세,이진택 대전대학교 산업기술연구소 1999 산업기술연구소 論文集 Vol.10 No.2

This paper explores the coefficient of consolidation using two-different test methods ; Constant Rate-of-Strain Consolidation Test(CRSC) and Standard Consolidation Test(SCT). Tests are conducted with 29 undisturbed soft clay sampled from 13 areas in South Korea. Total of 113 tests, 91 CRSC and 22 SCT, are performed. Index test to obtain water content, specific gravity, liquid and plastic limit of samples are also performed before and after the consolidation tests. The incremental loads from 0.05 kg/cm2 to 12.8 kg/cm2 are applied for SCT, and the speed of strain rate of 0.2 %/min. through 0.01 %/min. is adopted for CRSC. Regression analysis on the data obtained through the tests includes correlativity study between the coefficient of consolidation and soil index properties on the two different test methods.

자궁경부의 상피내암 조직내 In Situ Hybridization에 의한 Human Papillomavirus DNA의 검색 및 Typing

이상숙,김기권,정재홍,김택훈,이태성,진승원,손우익 대한의학협회 1990 대한의학협회지 Vol.33 No.1

스트렙토조토신 유발 당뇨병 백서에서 실험적 치아이동중의 치조골 교체

이기수,이택우,김성진 대한치과교정학회 2001 대한치과교정학회지 Vol.31 No.3

당뇨병 흰쥐를 대상으로 치조골의 교체(turnover)와 치아 이동 중 치조골의 흡수와 형성의 활성을 비교 관찰하기 위하여 8주령의 웅성 Sprague-Dawley계 흰쥐를 정상대조군(N), 당뇨군(D), 정상-치아이동군(N-tm), 당뇨-치아이동군(D-tm)으로 나누고, 각 실험군은 치아이동 실험 1일, 3일, 7일, 14일의 소군으로 나누었다. 당뇨병은 치아이동 실험 18일전에 스트렙토조토신(50mg/체중 kg)을 꼬리정맥에 단 회 주사하여 유발하였다. 치아이동은 상악 제1대구치를 초기 교정력 40그램으로 근심 이동시켰다. 상악 제1대구치 주위 치조골을 대상으로 골형성 징후표지로서 치조골 alkaline phos-phatase(ALP)와 osteocalcin(OC)을, 골흡수 징후표지로서 acid phosphatase(ACP)와tartrate-resistant acid phophatase(TRAP)을 정량 분석하였으며, D군과 N군에서는 이들의 혈중 농도를 정량 분석하였다. 1.D군의 혈청 TRAP농도와 혈청 OC농도는 모든 실험 경과군에서 N군보다 감소하였고(p<0.05), 당뇨기간이 길어질수록 더 많이 감소한 것으로 나타났다. 또한 치조골 흡수 징후표지는 감소하는 경향이었으며 골형성 징후표지도 실험기간이 경과함에 따라 더 많이 감소하는 것으로 나타났다. 2.N-tm군의 치조골 ACP농도와 TRAP농도는N군과 비교하여1일과3일군에서 가장 높았으며(p<0.01) 그 이후 감소경향을 나타냈고, 치조골 OC함량은 3일, 7일14일군에서N군보다 높았다(p<0.001), 그러나 D-tm군에서 치조골 ACP와 TRAP농도는 정상 N군에 비하여 1일에는 낮으나 7일, 14일 경과군에서 가장 높았고(p<0.01),실험 경과에 따라 계속 증가 하였으며(p<0.001), 치조골 ALP와 OC 함량은3일 경과 후부터 증가하지만 정상 N군과 유의차가 없었다. 3.당뇨-치아이동군의 치아이동량은 정상-치아이동군보다 많은 것으로 나타났다. 이러한 실험 결과는 당뇨병의 전신 골격과 치조골은 골 흡수 활성이 억제되어 있으며 골형성 활성은 당뇨 지속시간이 길어질수록 더 많이 억제되는 느린 골교체를 하며, 교정력에 의한 치조골 흡수활성 이 처음 높아지는 시기는 정상치아이동시보다 늦고 더 오랬 동안 지속되며 치조골 형성활성은 낮고, 치아 이동량은 증가하며 이동중 치아동요도가 증가함을 시사한다. 주요 단어 : 스트렙토조토신 유발 당뇨병, 치아이동, 치조골 교체, 인산효소, 오스테오칼신 The purpose of this study was to investigate the alveolar bone turnover in diabetic rat, and to compare the alveolar bone turnover during tooth movement in diabetes with that in normal control. Eighty Male Sprague-Dawley strain rats(8th week) were divided into normal control(N), normal-tooth movement (N-tm), diabetes(D), and diabetes-tooth movement(D-tm) groups. Eighteen days before the start of the experiment, diabetes was induce with a single injection of streptozotocin 50mg/kg of body weight in citrate buffer as vehicle via the tail vein. Maxillary first molars of rats were moved mesially by 40 grams of the closed coil spring. Experimental animals were sacrificed after 1d, 3d, 7d, and 14d experimental period, and the alveolar bone around the maxillary first molars were assayed biochemically for acid phsophatase(ACP) and tartrate-resistant acid phosphatase (TRAP) as bone resorption markers, and alkaline phosphatase(ALP) and osteocalcin(OC) as bone formation markers. TRAP and OC concentration in serum and alveolar bone of D group were lower than those in N group, and especially OC concentration decreased more following diabetes prolonged, which showed the decreased skeletal and alveolar bone resorption and formation potential in diabetic rats. In N-tm group compared with N group, alveolar bone ACP and TRAP concentrations were highest at 1d and 3d(p<0.01), decreased after then, and showed lowest at 14d, and alveolar bone OC concentration was higher at 3d, 7d, and 14d(p<0.001) and showed a tendency of peak level at 7d. which showed the peak of concentration of bone resorption markers at Id-3d and those of bone formation markers at 7d. In D-tm group compared with N group, alveolar bone ACP and TRAP concentrations were higher at 3d, 7d and 14d(p<0.001), and tended to reach peak value at 7d and persisted through l4d, and alveolar bone ALP and OC concentration increased but not different from that of N group. The amount of tooth movement in D group were greater than that of N group at all experimental period. Those results were suggested that during diabetes, the alveolar and skeletal bone undergo low bone turnover and the more amount of tooth movement, but because the peak time of alveolar bone resorption activity was delayed and sustained in longer period of tooth movement and alveolar bone formation activity is lower than that of normal tooth movement, the periodontal space is supposed to be larger during tooth movement. ※ Key words : Streptozotocin induced diabetes, Tooth movement, Alveolar bone turnover, Phosphatases, Osteocalcin

민족이산의 국제적 맥락과 한국의 대회관계

이기택 서울대학교 사회과학연구소 1984 社會科學과政策硏究 Vol.6 No.1

외교사난 국제정치사에서 네이션 스테이트(nation state)형성 또는 민족국가 형성이라고 하는 것을 근대사에서 살피면, 100년전 독일이 통일할 때, 또 이탈리아가 흩어진 자기네 민족을 결합, 통합하여 근대적인 통일국가를 형성하는 것이나, 또 섬나라로 형성되어 있었기 때문에 오랫동안 오토만(Ottoman)제국 하에서 민족이 흩어져 있었던 그리이스가 센트 페테스부르크(Saint Petersburg)나 런던(London)이나 파리(Paris)로 흩어졌던 민족들을 문화적인 전통을 가지고서 결합시켜 들어간 경우들이 있읍니다.

駐韓美軍撤收와 韓國의 安全

李基鐸 경북대학교 평화문제연구소 1978 평화연구 Vol.3 No.-

고전적 순막 조건화 동안의 해마 뇌전도와 다단위 활동

이두현,김현택,류재욱,김기석 한국심리학회 1990 한국심리학회지 생물 및 생리 Vol.2 No.1

해마에서만 발견되는 뇌전도는 낮은 주파수, 높은 진폭의 특징을 갖고 동기화가 잘 이루어져 마치 정현파에 가까운 형태를 보인다. 이러한 뇌전도에 대해 서파리듬 또는 θ파라 부르고 전기 생리학적인 연구가 많이 진행되고 있다. θ파의 주파수 특성, 발생기전, 행동과의 관련성 등에 대해 집중적 연구가 이루어졌고, 그러한 연구를 기초로 θ파의 기능에 관한 여러가설이 도출되었다. 본 연구에서는 이러한 해마의 서파리듬이 행동과 어떤 관계를 갖고 있는지를 알아보기 위하여 토끼의 순막반응을 고전적으로 조건화시키는 동안에 미리 해마에 삽입한 전극으로부터 뇌전도와 다단위활동을 기록하였다. 해마 서파리듬에 대해서는 주파수 분석을 실시하고 다단위 활동은 시간단위당 발화 횟수를 측정하여 조건화과정과의 관련성을 살펴보았다. 실험 결과, 해마의 다단위 활동은 순막의 조건 반응과 높은 상관관계를 보여주었다. 해마 서파리듬은 조건화 절차와 관계없이 조건자극, 무조건자극 제시 기간에 제2유형인 4,5㎐의 감소와 제1유형인 7,8㎐의 증가를 보였다. 이는 해마 서파리듬의 기능이 조건화된 반응보다는 조건자극과 무조건자극의 제시와 관련이 있음을 시사하는 것이었다. 이러한 결과를 해마 θ파의 기능에 관한 여러 가설에 비추어 논의하였다. This study was investigated to hippocampal θ rhythm and multiple unit response during classical conditioning of rabbit's nictitating membrane response. Hippocampal θ rhythm usually was classified into type 1 and type 2. According to various electrophysiological researches, type 1 θ rhythm was highly correlated with voluntary behavior and type 2 θ rhythm with automatic behavior, but there were studies which assert that type 1 θ rhythm was correlated with sensory information processing. So, this study was an attempt to investigate those results as compared paired conditioning group with unpaired control group. Paired group was presented 500msec tone CS followed 100msec air puff US after 400msec interstimulus interval. But unpaired group was presented 500msec tone and 100msec air puff independently. There was no difference in distinction of θ rhythm between the two group. Again, there was no correlation between θ rhythm and conditioned response. But, there was high correlation between multiple unit response and conditioned response. The result indicated that type 1 θ rhythm was highly correlated with presentation of tone and air puff in both group, this result suggest that type 1 θ rhythm is correlated with subject's information processing.

全歐洲安保會議와 MBFR

李基鐸 연세대학교 행정대학원 1973 延世行政論叢 Vol.1 No.-

교합력과 두개안면형태의 상관관계에 대한 연구

이택우,이기수 대한치과교정학회 1991 대한치과교정학회지 Vol.21 No.1

This study was undertaken to grope the correlation of the maximal bite force and toothcraniofacial structure. The maximal bite force of 76 adult male, aged 18-28(mean aged:23.4±2.2)years, was estimated and cephalometric headplates were measured, tabulated and statistically analyzed. The results were as follows. 1.59.61kg of bite force in first molar, 45.38kg in premolar and 17.10kg in central incisor were arranged. 2.The bite force was negatively correlated to gonial angle, mandibular plane angle, the angle between occlusal plane and mandibular plane, the angle between palatal plane and mandibular plane, and positively correlated to posterior height of face, length of mandibular body, length of ramus, facial depth in craniofacial structure. 3.The group with strong bite force showed small gonial angle, mandibular plane angle, the angle between occlusal plane and mandibular plane, the angle between palatal plane and mandibular plane, and long posterior height of face, length of mandibular body, length of ramus, facial depth. So they manifested the tendency to brachycephalic pattern, on the other hand, the group with weak bite force manifested the tendency to dolichocephalic pattern. 4.There is no correlationships between bite force and mesial inclination of premolar axis in this subject. 5.it is considered bite force have an effect upon craniofacial pattern, especially upon the lower face.

핀랜드의 中立外交政策(1914-1983)

李基鐸 동국대학교 유럽문화연구소 1983 유럽硏究 Vol.1 No.-