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Aspergillus niger로 부터 α-glucosidase 발현억제 형질전환체의 분리
이동건,이진영,서영배 한국미생물생명공학회 ( 구 한국산업미생물학회 ) 1996 한국미생물·생명공학회지 Vol.24 No.4
We have already cloned an extracellular α-glucosidase gene from Aspergillus niger with oligonucleotide probe synthesized on the basis of the peptide sequences determined previously. The DNA sequence revealed an open reading frame of 895 amino acids split by three introns. We are attempting to construct an A. niger strain deficient in the α-glucosidase enzyme activity, which would be useful for the glucoamylase production without contamination by the industrially undesirable α-glucosidase. For destruction of the α-glucosidase gene, we try to make transformations. A cloned partial α-glucosidase gene was introduced into Aspergillus niger, and transformants with suppressed α-glucosidase activity were isolated. The transformants were cultured on YPD medium which contained Hygromycin B at 30℃. The activity of α-glucosidase of the suppressed transformants was compared to that of wild type activity. As shown by southern-hybridization, we detected that the transformant was a heterocaryon.
Effect of Chlorella vulgaris on Immune-enhancement and Cytokine Production in vivo and in vitro
Hyo-Jin An,Hong-Kun Rim,Jong-Hyun Lee,Min-Jun Seo,Jin-Woo Hong,Na-Hyung Kim,Noh-Yil Myung,Phil-Dong Moon,In-Young Choi,Ho-Jeong Na,Su-Jin Kim,Hyun-Ja Jeong,Hyeung-Suk Park,Jae-Gab Han,Jae-Young Um,Seu 한국식품과학회 2008 Food Science and Biotechnology Vol.17 No.5
The object of this study was to investigate the immune-enhancing effects of Chlorella vulgaris (CV) on a deteriorated immune function by a protein-energy malnutrition (PEM) diet. Unicellular algae, CV were used as a biological response modifier. Male C57BL/6J mice were fed for 15 days with standard diet or a PEM diet, which is associated with decreased host immune defense. After 8 days, mice in the PEM diet group were orally administered by 0.05, 0.1, and 0.15 g/kg body weight of CV or distilled water. Nutritional parameters, and interferon (IFN)-γ levels were significantly increased in the blood serum of the CV (0.15 g/kg)-treated group (29.6±2.8 pg/mL) compared to the non-treated PEM group (4.1±0.4 pg/mL, p<0.05). In addition, cell proliferation and production of cytokines were investigated via a CV (0.01, 0.1, and 1 mg/mL) treatment using a human T cell line MOLT-4 cell. The CV treatment (1 mg/mL) significantly increased the production of both IFN-γ and interleukin (IL)-2 (51.3±3.4 and 285.9±18.8 pg/mL, respectively) compared to the control (51.3±3.4 and 442.6±14.3 pg/mL, respectively), but did not affect the production of IL-4. These results suggest that CV may be useful in improving the immune function.
Poster Session : PEP-1-Cu,Zn-superoxide dismutase (SOD) mutants transduced into neuronal cells
( Jae Jin An ),( Seok Il Hwang ),( So Young Kim ),( Sun Hwa Lee ),( Dae Won Kim ),( Jung Hoon Kang ),( Jin Seu Park ),( Won Sik Eum ),( Soo Young Choi ) 한국생화학분자생물학회 (구 한국생화학회) 2006 생화학분자생물학회 춘계학술발표논문집 Vol.2006 No.-
Park, Jin-Seu,Lee, Byung-Ryong,Jin, Li Hua,Kim, Choong-Kwon,Choi, Kyung-Soon,Bahn, Jae-Hoon,Lee, Kil-Soo,Kwon, Hyeok-Yil,Chang, Hyun-Woo,Baek, Nam-In,Lee, Hwang-Eunjoo,Kang, Jung-Hoon,Cho, Sung-Woo,Ch Korean Society for Biochemistry and Molecular Biol 2001 Journal of biochemistry and molecular biology Vol.34 No.2
Antioxidant enzymes, scavengers of the reactive oxygen intermediate (ROI), are involved in numerous defense systems in cells. In the present study, we investigated the effects of the hot-water extracts of two medicinally potent mushrooms (Ganoderma lucidum and Phellinus linteus) on the activity and expression of antioxidant enzymes in vitro and in vivo. The mushroom extracts stimulated the catalase activity in a dose-dependent manner in vitro, whereas the other antioxidant enzymes (such as superoxide dismutase (SOD), glutathione peroxidase (GPx)) were unaffected by the extracts. The catalytic activity of catalase in the liver and brain was significantly increased after the oral treatment of the mushroom extracts (2.5 g/kg) to ICR mice for 2 months. Western blot analysis of the liver and brain tissues revealed that the expression level of catalase in the mice, treated with both mushroom extracts, was significantly increased compared to that of the control mice. However, the level of the SOD expression in the mice treated with the natural product extracts was unchanged under the same experimental conditions. Although the mechanisms for the stimulatory effect of the catalase expression by these extracts remains unclear, these results suggest that the ingredients of the Ganoderma lucidum and Phellinus linteus extracts act as an activator of catalase, and regulate the expression of catalase at the translational or transcriptional level.