조혈모세포이식 후 골성장인자의 변화 및 골대사에 미치는 영향 : Impact on Bone Mineral Metabolism

백기현,오은숙,오기원,이원영,김혜수,권순용,한제호,강무일,차봉연,이광우,손호영,강성구,김춘추 대한내분비학회 2002 Endocrinology and metabolism Vol.17 No.5

연구배경: 각종 장기이식의 시행이 많아지고 이식 후 생존율이 증가함에 따라 이식 후 합병증에 대한 관심 또한 높아지고 있다. 조혈모세포이식 후에도 다양한 내분비적 합병증이 발생할 수 있으며 골격에 대한 합병증도 문제점으로 대두되고 있다. 조혈모세포 이식 후 발생하는 골소실에는 이식 후 초기의 골형성 저하와 골흡수 증가가 중요한 역할을 담당하리라고 추측되는데 이러한 골재형성불일치(biochemical uncoupling)에 골 성장인자들이 미치는 영향에 대해서는 알려진 바가 없다. 본 연구에서는 조혈모세포이식 전, 후로 말초 혈액에서 IGF-I, FGF-2, M-CSF같은 성장인자의 변화를 알아보고, 이들 성장인자의 변화가 조혈모세포이식 후의 골형과 골흡수에 미치는 영향 및 이식 후 발생되는 골량 소실과의 연관성을 확인해 보고자 하였다. 방법: 여러 가지 혈액질환으로 인해 동종 골수이식을 시행 받은 환자들을 전향적으로 관찰하였으며 이식 전 및 이식 후 1주, 2주, 3주, 4주 및 3개월, 6개월 1년에 말초 혈액에서 골교체표지자를 측정하였다. 이식 전 및 이식1년 후에 요추골 및 대퇴골 골밀도를 측정할 수 있었던 36명의 환자들을 대상으로 냉동 보관되어 있던 혈청을 이용하여 IGF-I, FGF-2 및 M-CSF를 시기별로 측정하였으며 이들 성장인자와 골교체표지자의 변화 및 골밀도 변화 사이의 상관관계를 확인하였다. 결과: 골흡수 표지자인 혈청 ICTP는 이식 전에 비해 이식 후 4주까지 점차 의의 있게 증가하다가 이후 6개월까지 더욱 증가한 후 감소하였다. 골형성 표지자인 osteocalcin은 이식 후 3주까지는 점차 감소하다가 이후 증가하여 이식 후 3개월 및 6개월에 기저치보다 통계적으로 유의하게 증가한 후 감소하였다. 혈청 IGF-I과 FGF-2는 각각 이식 후 3주 및 1주까지 의미있게 감소하다가 이후 증가하였으며 혈청 M-CSF는 이식 후 1주째에 기저치에 비해 의미 있게 증가하였다가 이후 기저치로 회복되었다. 이식 1년 후 평균 요추부 골밀도는 5.2% 감소하였고 평균 근위대퇴골 골밀도는 11.6% 감소하였다. 이식 전 및 이식 후 3주에 측정한 IGF-I과 같은 시기에 측정한 오스테오칼신 사이에 유의한 상관관계가 관찰되었으며 이식 후 3주째의 M-CSF와 골흡수표지자인 M-CSF 사이에서 의미 있는 양의 상관관계를 관찰할 수 있었다. 이식 후 3주 및 3개월에 IGF-I이 낮은 환자일수록 이식 1년 후 근위대퇴골에서의 골소실이 많은 것으로 분석되었다. 결론: 조혈모세포이식 후 발생하는 골소실에는 기존에 알려진 기저질환의 영향, 성호르몬의 감소, 면역억제의 투여, 골수기질세포와 조골세포의 손상 및 이식초기 사이토카인의 변화이외에도 골성장인자가 관련이 있음을 확인하였고, 이는 이식 후 발생되는 골량소실에 중요한 역할을 할 것이라고 사료된다. Background: A loss of bone mass is usually detected after a bone marrow transplantation (BMT), especially during the early post-transplant period. We recently reported that enhanced bone resorption following a BMT was related to both the steroid dose and the increase in IL-6. We also suggested damage to the marrow stromal microenvironment, by myoablation, partly explains the impaired bone formation following a BMT. It is well known that some growth factor play important role in bone growth and osteogenesis. However, the pathogenetic role of bone growth factors in post-BMT bone loss is unknown and data on the changes in the growth factors, in accordance with bone turnover markers and bone mineral density (BMD) changes are scarce. We investigated changes in bone growth factors such as IGF-I (Insulin-like growth factor-I), fibroblast growth factor-2 (FGF-2) and Macrophage colony stimulating factor (M-CSF), during the post-BMT period, and assessed whether the growth factor changes influenced the bone turnover and post-BMT bone loss. The present study is the first prospective study to describe the changes in bone growth factors following a BMT. Methods: We prospectively investigated 110 patients undergoing a BMT, and analyzed 36 patients (32.4±1.3 years, 17 men and 19 women) whose BMDs were measured before, and 1 year after, the BMT. The serum biochemical markers of bone turnover were measured before, 1, 2, 3 and 4 weeks, 3 and 6 months, and 1 year, after the BMT. The serum, FGF-2, IGF-I and M-CSF levels were measured before and 1 and 3 weeks, and 3 months after the BMT. The correlation between the changes of growth factors and various bone parameters was analyzed. Results: The mean bone losses in the lumbar spine and total proximal femur, calculated as the percentage change from the baseline to the level at 1 year, were 5.2(p<0.05) and 11.6%(p<0.01), respectively. the serum type I carboxyterminal telopeptide(ICTP), a bone resorption marker, increased progressively until 6 months after the BMT, but thereafter decreased, to the base value after 1 year. Serum osteocalcin, a bone formation marker, decreased progressively, until 3 weeks after the BMT but then increased transiently, and finally returned to the base level at 1 year. The serum IGF-I and FGF-2 also decreased progressively until 3 weeks 1 week after the BMT, respectively, then increased to the base values at 3 months. The serum M-CSF increased briskly at 1 week post-BMT, then decreased to the base level. There were positive correlations between the percentage changes from the baseline proximal femur BMD and the IGF-I levels 3 weeks and 3 months (r=0.52, p<0.01, r=0.41, p<0.05) post BMT. A significant correlation was found between the IGF-I and osteocalcin levels pre-BMT, and 3 weeks after the BMT. Another positive correlation was found between the M-CSF and the ICTP levels at 3 weeks post BMT (r=0.54, p<0.05). Conclusion: In conclusion, there were significant changes in the serum IGF-I, FGF-2 and M-CSF levels in the immediate post-BMT period, which were related to a decrease in bone formation and loss in the proximal femoral BMD during the year following the BMT (J Kor Soc Endocrinol 17:664∼674, 2002).

골수이식 후 골밀도 및 골대사의 변화 : 2년 추적관찰 2-Year Prospective Study

이원영,강무일,오은숙,오기원,한제호,손현식,윤건호,차봉연,이광우,손호영,강성구,신완식,민우성,김춘추 대한내분비학회 2000 Endocrinology and metabolism Vol.15 No.4·5

조혈모세포이식술이 골수기질세포의 기원 및 조골세포로의 증식 및 분화에 미치는 효과

강무일,조성원,오은숙,백기현,이원영,오기원,김혜수,한제호,윤건호,차봉연,이광우,손호영,강성구,김춘추 대한내분비학회 2000 Endocrinology and metabolism Vol.15 No.4·5

First Record of Xanthogaleruca aenescens Fairmaire, 1878 (Coleoptera: Chrysomelidae: Galerucinae) from S. Korea

Jinyoung Park,Jin Yeol Cha,Ik Je Choi,Jong Eun Lee,Jong Kyun PARK 한국응용곤충학회 2015 한국응용곤충학회 학술대회논문집 Vol.2015 No.04

The Chrysomelid species of the genus Xanthogaleruca, X. aenescens Fairmaire, 1878 is reportd for the first time from South Korea. The genus Xanthogaleruca Laboissiére, 1934, within subfamily Galerucinae, contains only nine species, and is distributed Palearctic region (Beenen, 2010), but one adventive species, X. luteola (Müller, 1766), is widespread throughout much of Canada, North Africa and the United States. Also known as the elm leaf beetle, it feed on Ulmus (Ulmaceae). In the present study we report on the genus Xanthogaleruca Laboissiére, 1934 from Korea for the first time, based on X. aenescens Fairmaire, 1878. We provide the habitus photographs, illustrations of diagnostic features and a key to the genus in S. Korea.

Intensity Change of Tropical Cyclones over the Western North Pacific with Respect to the RSMC-JTWC Comparison

Eun-Hee Baek,H. Joe Kwon,Yun-Je Lee,Seung-Hoon Lee,Chan-Soo Kim,Eun-Jeong Cha 건국대학교 기후연구소 2011 기후연구 Vol.6 No.1

This paper reexamines the interannual intensity change of tropical cyclone (TC) over the western North Pacific. Analyses are done for the annual extreme intensity and the average intensity of the five strongest typhoons based on the best track data issued from the RSMC (Regional Specialized Meteorological Center) Tokyo and the JTWC (Joint Typhoon Warning Center). Trends over the last 30+ years in one data set are nearly opposite in another. Specifically, the TC intensity tends to increase with the years in the JTWC data, whereas the opposite is found in the RSMC data. Realizing that one data set shows a significant statistical difference from the other, a homogenization process has been applied to the original data so that comparisons of the two data sets and the trend analyses become more meaningful than those with solely the original data. With the homogenized data, we present some cautious conclusions regarding the TC intensity changes over the western North Pacific. The annual extreme TC intensity has been decreasing, while the strong TCs have become stronger over the years. For TCs entering the KMA emergency area and directly hitting Korea, we are hesitant to make any conclusions because the corresponding area is small so that the number of TCs entering the area each year is too small to have any statistically significant implications.

Soil Characterization of the Field where Rice has been Cultivated during Five Years

Eun-Jin Cha(차은진),Jin-Kyeong Lee(이진경),Min-Ho Jang(장민호),Min-A Choi(최민아),Jae-Hyun Kim(김재현),Seung-Je Han(한승재),Jin-Hee Park(박진희),Chang-Seop Shin(신창섭) 한국기계가공학회 2021 한국기계가공학회지 Vol.20 No.2

The study for soil has been conducted separately by several areas such as soil mechanics and soil chemistry. Soil is important in terms of prediction of how the plant grow with nutrient requirement. Also, soil is important for machines to work on to solve labor shortage and save farmers from harsh environment during farm work. To meet diverse needs related to soil in agriculture, the soil related study needs to be conducted synthetically. Thus, we tried to obtain the data related to soil chemistry including pH and Electrical Conductivity (EC) with data related to soil mechanics including Cone Index (CI), moisture content, soil classification. Specifically, the condition of the field was set to be cultivated at least for five years continuously at a first step. The soil was taken from 30 sites. CI was obtained using the soil penetrometer and soil classification was conducted using sieve analysis with eight kinds of sieve. The soil was taken on December when is during winter in Korea. There was variation of data including moisture content and CI.

Identification of Putative Serum Glycoprotein Biomarkers for Human Lung Adenocarcinoma by Multi-Lectin Affinity Chromatography and LC-MS/MS

( Eun Jin Kim ),( Sung Ick Cha ),( Chang Ho Kim ),( Tae Hoon Jung ),( Jae Yong Park ),( Sun Hee Heo ),( Seung Jin Lee ),( Je Yoel Cho ) 대한결핵 및 호흡기학회 2007 대한결핵 및 호흡기학회 추계학술대회 초록집 Vol.105 No.-

엔드밀 가공을 이용한 스페큘러 홀로그램 제작

전은채(Eun-chae Jeon),차진호(Jin-Ho Cha),이재령(Je-Ryung Lee),최환진(Hwan-Jin Choi),김창의(Chang-Eui Kim),제태진(Tae-Jin Je),김휘(Hwi Kim),최두선(Doo-Sun Choi) 한국기계가공학회 2014 한국기계가공학회지 Vol.13 No.4

The specular hologram is one type of hologram, and it consists of many arcs. They are very easy to fabricate and can even be machined by hand had a compass. In this study, we designed two squares having different depths and consisting of many arcs, after which we machined the arcs using end-mill technology. The width of the machined arcs showed high repeatability. Moving tracks were observed on the bottom surface, and top burrs were noted. In spite of them, the phenomenon of the specular hologram was observed when an observer and a light source stood on the same side. The two squares seemed to have different depths when they were observed from the left and right directions. In this study, it was verified that a specular hologram can be manufactured by end-mill technology.

Mesenchymal Stem Cell-Derived Exosomes Attenuate TLR7-Mediated Mast Cell Activation

조경아,Cha Je-Eun,Kim Jungwoo,김유희,유경하,Woo So-Youn 한국조직공학과 재생의학회 2022 조직공학과 재생의학 Vol.19 No.1

BACKGROUND: Mast cells are immune sentinels in the skin that respond to a wide range of pathological and environmental stimuli; they owe their function to the expression of Toll-like receptors (TLRs). We previously found that tonsilderived mesenchymal stem cells (T-MSCs) were able to effectively attenuate TLR7-mediated skin inflammation in mice, which was accompanied by an increase in mast cell number. The present study investigated whether T-MSC extracellular vesicles, such as exosomes, are able to regulate mast cell activation in response to TLR7 stimulation. METHODS: The HMC-1 human mast cell line was treated with a TLR7 agonist in the presence or absence of T-MSC exosomes, and the levels of expressed inflammatory cytokines were assessed. Additionally, mice were repeatedly injected with a TLR7 agonist with or without interval treatments with T-MSC exosomes and assessed dermal distribution of mast cells and related immune cells. RESULTS: We showed that T-MSC exosomes containing microRNAs that target inflammatory cytokines significantly reduced the expression of inflammatory cytokines in TLR7 agonist-treated HMC-1 cells. In addition, T-MSC exosomes inhibited the increase in the number of both dermal mast cells and CD14-positive cells in TLR7 agonist-treated mice. CONCLUSION: Our data suggest that T-MSC exosomes have regulatory effects on mast cell activation under inflammatory conditions, including TLR7 stimulation.

자궁 경부 원추 생검 상 자궁 경부 상피내 종양 3 이면서 절제면 양성인 환자들의 처치

이은주(Eun Ju Lee),이선주(Sun Ju Lee),차경봉(Kyong Bong Cha),김형선(Hyoung Sun Kim),박창수(Chang Soo Park),이제호(Je Ho Lee),배덕수(Duk Soo Bae) 대한산부인과학회 2001 Obstetrics & Gynecology Science Vol.44 No.9

Objective : To evaluate conservative management of patients with cervical intraepithelial lesion, grade III (CIN III) and positive resection margins after cervical conization. Methods : We reviewed records of 64 patients undergoing cervical conization revealing CIN III with positive resection margins between January 1997 and December 1999. Thirty-three (51.5%) patients underwent conization only and thirty-one (48.5%) underwent conization and subsequent hysterectomy. These patients were followed up with a Pap smear every 3-6 months and then persistence and recurrence were determined in each group. The sites of incomplete excision and HPV infection at cone and residual lesion at hysterectomy specimen were evaluated. Results : Thirty-one of 33 patients receiving conservative management and all 31 patients receiving subsequent hysterectomy had negative Pap smear (P=0.262). In the group of the conservative management, one case of 11 patients with extension to the endocervical margin had positive Pap smear (P=0.577). One of 17 cases with HPV infection and one of 10 cases with negative HPV testing had positive cytology (P=0.630). In the group of subsequent hysterectomy, nine cases (42.86%) of 21 patients with extension to endocervical margin and two (22.22%) of 9 patients with extension to exocervical margin had residual lesion in hysterectomy specimen (P=0.258). Only one (8.33%) of 12 cases with positive HPV and five (50%) of 10 patients with negative HPV had residual lesion (P=0.043). Conclusion : Patients with CIN III and positive resection margins after cervical conization can be followed appropriately with serial cytology. Endocervical margin involvement and HPV infection were not statistically significant predictors of residual lesion, persistence and recurrence in our patients.