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생약추출물 유도형 Nitric Oxide Synthase 저해활성 검색
류재하,이소영,박재현,이화진,장세란,은진희,김남이,정연수,장미경,최영은,이숙현,손행자,안한나,고혜진 숙명여자대학교 약학연구소 2001 약학논문집-숙명여자대학교 Vol.17 No.-
Nitric Oxide (NO), derived from L-arginine, is produced by two types (constitutive and inducible) of nitric oxide synthase (NOS: cNOS and iNOS). The NO produced in large amounts by the iNOS is known to be responsible for the vasodilation and hypotension observed in septic shock and inflammation. The inhibitors of iNOS, thus, may be useful candidate for the treatment of inflammatory diseases accompanied by the overproduction of NO. We prepared alcoholic extracts of herbal drugs which have been used for the treatment of inflammation in oriental medicine. We have screened the inhibitory activity of NO production in lipopolysaccharide (LPS)-activated macrophages after the treatment of these extracts. Among the 81 kinds of extracts of herbal drugs, 34 extracts showed potent inhibitory activity of NO production above 50% at the concentration of 50 (μg/ml. The inhibitory activities of NO production were also evaluated for several solvent fractions at three different concentrations. Especially, hexane soluble fractions of Agrimonia pilosa, Hydrangea serrata, Machilus thunbergii, Prunella vulgaris, Saussurea lappa, Tussilago farfara, and ethyl acetate soluble fractions of Angelica gigas, Ostericum koreanum, Torilis japonica, Perilla frutescence showed moderate activity at 10 and/ or 25 (μg/ml. These fractions are promising candidates for the study of the activity-guided chromatographic purification of active compounds.
사철나무 알칼로이드, Euojaponine의 NMR스펙트럼의 분석
柳載夏,李沼映,李叔淵,韓秉勳 숙명여자대학교 약학연구소 1994 약학논문집-숙명여자대학교 Vol.10 No.-
The strutures of euojaponines, alkaloidal components from Euonymus japonica are composed of β-agarofuran type sesquiterpene and pyridic dibasic acid, and they are linked via cyclodiester bonds. The structures of these compounds were elucidated by the application of various two dimensional NMR methods. From the results of complete interpretation of NMR spectra, several correlating aspects between structure and spectral pattern of sesquiterpene moiety were found, which can be utilized for the structural determination of the same family compounds.
류은석,김윤숙,전섭,김종수,최승도,선우재근,배동한,양승화 순천향의학연구소 2005 Journal of Soonchunhyang Medical Science Vol.11 No.1
Fibrothecoma are mesenchymal tumors deriving from the ovarian stroma and consisting of theca-like elements and fibrous tissues. The reported incidence is between 0.4 and 8.0% of all ovarian tumors and this side variability can be attributed to the difficulty in differentiation fibrothecoma from ovarian fibroma or hyperplastic stroma nodules. We experienced a rare case of ovarian fibrothecoma in woman at 38 weeks gestation with previous repeat cesarean section diagnosed after tertiary cesarean delivery and bilateral tubal ligation with a brief review of literature.
요하지통 환자에서 두 개의 캐눌라를 이용한 박동성 고주파 신경근 응고술의 임상 연구
김천숙,배재영,배덕구,강규식,안기량,권진형,김지은,유시현 순천향의학연구소 2003 Journal of Soonchunhyang Medical Science Vol.9 No.1
Dorsal root ganglion(DRG) block by the local anesthetics and steroids which has done classically in low back pain(LBP) patients with radiculopathy has high incidence of recurrence rate and side effects of steroids. Recently a new technique of pulsed radiofrequency thermocoagulation(P-RFTC) was introduced and substituted for it because of the benefits such as relatively low recurrence rate, low risk of nerve injury and comfort during the procedure. We experienced 15 patients who had LBP with radiculopathy and no or little response to epidural steroid injection. When we performed DR ganglionotomy by P- RFTC under the C-arm guide in these patients, we tried to approach the exact DR ganglion using tow cannulas - the one for obtaining patient's subjective symptoms and injection of contrast dye, the other for making a RF lesioning after identifying the impedance of sensory and motor stimulation. In conclusion, DR ganglionotomy by P- RFTC using two cannulas in LBP patients is an easy and safe procedure with satisfactory results.
질트리코모나스(Trichomonas vaginalis)의 효소활성에 관한 전자현미경적 관찰
민득영,류재숙,박건채 한양대학교 의과대학 1992 한양의대 학술지 Vol.12 No.2
Trichomonas vaginales is one of the parasitic flagellates found in the vaginal cavity of female and the urogenital tract of male. The presnet study was aimde to evaluate the effect of antiserum from immunized Wkstar rats on activities of acid phoshpatase (AcPase) and adenosine triphosphatase (ATPase) of T. vaginalis. Trophozoites were subcultured in Diamond's TPS-1 medium at 37℃ in CO₂ incubator. T. vaginalis were incubated in mixed medium containing 26%(v/v) of antiserum for minutes, then treated with substrates for enzyme activityes. Parasites were embedded in Epon and sectioned for transmission electron microscopy. The results observed are as follows: 1. AcPase activities. In normal prarsites strong activities were observed on Golgi complex and a few of wacuoles. Weak reactions also observed in nuclear matrix, kinetosome and hydrogenosome. In cases treated with antiserum plasma membrane were parially destroyed. Vacuoles were increased in number and revealed strong reactivites. 2. ATPase activities. In normal parasites ATPase were observed dominantly in nucleus. In parasites treated with antiserum activities in nucleus were stronger than those of normal ones, and weak activities were seen along plasma membrane. In conclusion it is assumed that antiserum does increase AcPase activity, but reduces ATPase activity in T. vaginalis.
면역시킨 마우스 흉선 및 비장세포에 대한 병원성 Naegleria의 세포병변 효과
안명희,류재숙,민득영 한양대학교 의과대학 1991 한양의대 학술지 Vol.11 No.1
Naegleria fowleri is a causative agent of primary amoebic meningoencephalitis (PAM) in man and experimental animal. We investigated the interaction of immunized mouse thymocyte and splenocyte with N. fowleri trophozoites in vitro. BALb/c mouse was immunized with 150∼200×10⁴/ml N. fowleri trophozoites, intraperitoneally, once a week, for two times. Thymus and spleen cells from immunized or normal mouse cocultured with N.fowleri in 10% FCS+MEM, 37℃, 55%-CO₂incubator and cell: amoeba ratio was 200∼400:1. Thymus or spleen cells and N. fowleri were observed at 24, 48, 72 hrs after incubation by inverted microscope. Spleen cells from immunized mice showed moderate lysis aftre 72 hrs with round amoeba, but other group cells were completely lysed at 48 hrs after incubation with N. fowleri. It may be secreted a certain cytolytic amterial from N. fowleri because of cell lysis occured abruptly after 24 hrs culture. And amoebae showed better condition i.e., pseudopodaand vacuoles, after phagocytosis of lytic cell material. On the other hand, nonadherent cells from immunized spleen were completely lysed at 48 hrs with N. fowleri conculture and immunized thymus cell with aditive activated mactophage showed prolonged cell survival. And so, cooperative reaction of immunized T-lympocyte and macrophage are important to supperss N. fowleri in vitro.
항혈청으로 처치된 질트리코모나스(Trichomonas vaginalis) 미세구조의 변화
민득영,류재숙,안명희,박승정,조휘율 한양대학교 의과대학 1994 한양의대 학술지 Vol.14 No.2
Trichomonas vaginalis is a parasitic flagellate found in the urogenital tract of humans. The present study was performed to evaluate the effect of antiserum from rat and rabbit on fine structure of T. vaginalis. Trophozoites were subcultured in TPS-1 medium(Diamond, 1968) at 37℃, CO₂incubator. T. vaginalis were inoculated in mixed medium containing of antiserum for 10 and 30 minutes, then observed with transmission and scanning electron microscope. The results are as follows: 1. T. vaginalis was ellipsoidal or ovoidal in shape with 4 flagella, an undulating membrane and an axostyle. The plasma membrane of body surface was smooth and furrowed. Pseudopodia(filopods) were often observed by SEM. Ultrastructure of trophozoites consisted of an elliptical shaped nucleus, RERs and Golgi complex around the nucleus, hydrogenosomes, glycogen granules, polysomes and vacuoles in cytoplasm. As the organelles for supporting and movement, an axostyle, kinetosomes, flagella, undulating membrane, costa and parabasal filaments were observed. 2. T. vaginalis treated with antiserum, its surface appeared destruction of plasma membrane, alteration and/or disappearance of axostyle, flagella and undulating membrane under the SEM and showed fine structural changes such as the nucleus surrounded by many vacuoles, decreased ribosomes, which attached to nuclear membrane and RER, vesiculated RER and Golgi complex. The area of filamentous cell coat and a number of coated vesicles were increased on plasma membrane with partial damage. According to going by the incubation time, the progressive destructions of cytoplasmic organelles, nuclear membrane, hydrogenosomal membrane, kinetosome and rootlet fiber were noted. With above results, it is presumed that specific antiserum induced the disturbance of protein systhesis and the damage of cytoplasm resulting lysis of T. vaginalis.