ATB Expert System을 이용한 항균제 감수성 검사에 관한 연구

구선회 충남대학교 의과대학 지역사회의학연구소 1993 충남의대잡지 Vol.20 No.2

The success of therapy depends on the selection of antibiotics by the clinician in the case of infection. performance of susceptibility test provides the clinician with a prediction of the activity of an antibiotics. but nowadays resistance mechanisms of increasing complexity are found in a growing number of microbial species. So, for the validation of the antibiotic susceptibility requires the more complete interpretation, using all the knowledge accumulated on antibiotic resistance mechanism. we could detect the resistance mechanism, such as acquire resistance, crossresitance, associated resistance by the ATB expert system which was deviced by artificial knowledge. also we could detect the technical error, incomplete expression of resistance, rare phenotype and impossible phenotype. but there are some limitation for complete adoption in clinical fields because only a few rules were used and not so simple for all members of the microbiology laboratory.

형광 염색체 In Situ Suppression Hybridization의 임상적 응용에 관한 연구

구선희 충남대학교 의과대학 지역사회의학연구소 1992 충남의대잡지 Vol.19 No.2

A method for chromosome specific staining and its use in clinical cytogenetics is evaluated. Non isotopic fluorescent in situ hybridization provides a fast method for detection of specific nucleic acid sequences. We have used the single color fluorescent in situ hybridization with a α-satellite probe, unique sequence, microdissected probe to investigate the feasibility of simultaneous assessment of numerical and structural chromosome aberrations. When we used the α-satellits probe, Under condition of low stringency, cross hybridization with other subpopulations of the alpohoid family occurs in the whole chromosome and numerous hybridization sites are detected over metaphase. But we could findout the 3 signals in interphase chromosome of Down syndrome we could detect the signals which have been suspicted a deletion point with the unique sequence probe. We could delineate the X-Y translocated region by a microdisscted Y probe. From the above results, it can be a novel method for detection of cytogenetic abnormalities in the clinical cytogenetic field.

CALPUFF model과 ISCST3 model을 이용한 악취모델링 기법 연구

구윤서,김성태,김용규,송선호 안양대학교 산업기술연구소 2002 自然科學硏究 Vol.9 No.-

악취는 인간의 후각기관에 의해 감지되기 때문에, 대상 지역의 평균농도(Average Concentration) 보다는 악취의 특성상 순간적인 농도인 첨두농도(Peak Concentration)가 중요한 의미를 갖는다. 따라서 이것을 모델링에 적용하기에는 많은 어려움이 있다. 이를 해결하는 것이 이 연구의 주된 과제라 할 수 있다. 현재 국내에서 환경영향평가에 많이 사용되고 있는 ISCST3 Model (Industrial Source Complex Short Term 3 Model)과 CALPUFF Model (California Puff Model)을 사용하여 첨두농도(Peak Concentration)를 고려한 관능악취도(Odor Intensity)를 두 가지 방법으로 산정 하였다. 첫 번째로는 오염원에서 배출되는 물질의 농도를 이용하는 방법과 다른 하나는 종합악취도 개념으로 악취농도(Odor Concentration)를 이용하여 관능악취도를 산정하는 것이다. 위의 두 가지 방법을 ISCST3 Model과 CALPUFF Model에 적용하여 모델링을 수행한 후 나온 기여농도를 첨두농도로 환산한 후 관능악취도를 계산하였다. 이와 같은 이론 배경을 바탕으로 현장 적용성을 파악하기 위해서 수도권매립장의 한 지점에서 가상의 가스배제공이 있다고 가정하고, ISCST3 Model과 CALPUFF Model에 동일한 조건으로 모델링을 수행하여 매립장 주변에 미치는 관능악취도를 각각 산출해 비교 검토하였다. 그 결과 두 모델에서의 차이는 크지 않았으나 기여농도와 첨두농도 사이에는 상당한 차이를 보였다. 결과적으로 악취모델링을 수행함에 있어서 기여농도만을 고려하는 것은 관능악취도를 과소평가 할 우려가 있으므로 배출원 특성을 고려한 첨두농도로 관능악취도를 평가하는 것이 타당하다고 판단된다. In order to manage the odor related public complains effectively, the estimation of peak ground-level concentration which is capable of predicting the intermittent maximum response less than 10 seconds is very important. However, peak rather than 3 minute or 1 hour average concentrations are not easily predicted by current air dispersion models such as SCST3 (Industrial Source Complex Short Term - version 3) and CALPUFF (CALifornia Puff) models. According to the field and wind tunnel measurements, the intensity of the peak concentration depends on the emission source type, downwind distance and atmospheric stability. The odor modeling methods to predict the peak concentration were reviewed and the detailed procedure for the odor modeling was explained in this study. In order to compare the conventional air dispersion models with the oder models, the odor concentrations with various modeling techniques under the identical emission and meterological conditions were calculated. The peak concentration shows highest value than the 3 minutes or 1 hour average concentrations. The predicted odor concentrations by the CALPUFF model are higher than those predicted by the ISCST3 model since the CALPUFF model reflects the time-dependent movement of the plume. It is recommended that using the peak concentration with the CALPUFF model rather than 3 minutes or 1 hour average concentrations is more reasonable approaches to manage the odor problems.

창의적 문제해결을 위한 웹기반 교수-학습 모형과 학습 환경 설계 : 수학교과에서의 예시를 중심으로

구양미,김영수,노선숙,조성민 이화여자대학교 사범대학 교과교육연구소 2006 교과교육학연구 Vol.10 No.1

본 연구의 목적은 지식기반사회의 학습도구로서 그 중요성이 증대되고 있는 인터넷과 웹을 활용하여 창의성 계발을 돕는 교수-학습 모형과 웹기반 학습 환경을 설계하는 데 있다. 본 연구에서 웹은 학습도우미(learning agent)로서 문제해결 학습상황에서 의사소통도구, 발견과 탐색도구, 지식과 경험 구성의 도구로 상정되었다. 즉, 중등 수학교과를 사례로 채택, 문제해결 학습상황에서 생각 5단계 모형(생각 열기, 생각 쌓기, 생각 나누기, 생각 다지기, 생각 펼치기)을 토대로 각 단계별로 문제의 구조 유형에 따른 사고 전략과 이에 상응하는 학습자와 교수자 활동, 웹기반 활동을 포함하는 웹기반 교수-학습 모형을 설계하였다. 설계된 웹기반 교수-학습 모형에 따라 실제 교수-학습활동 계획안을 개발하고, 창의적 문제해결 환경 지원을 목적으로 한 웹사이트를 설계하였다. 본 연구의 결과물인 웹기반 교수-학습 모형과 학습 환경은 창의적 문제해결을 위한 핵심요소로 간주되는 확산적 사고, 비판적 사고 및 교과지식이 조화롭게 활용될 수 있도록 하는데 그 주안점을 두고 있다. The purpose of this study is to design a web-based teaching and learning model and learning environment for creative problem solving in Mathematics. In this study, World Wide Web is considered as a learning agent assisting learning process as communication tools, inquiry tool, and knowledge construction tools in problem solving learning environments. The web-based teaching and learning model was developed based on "Five Stage Thinking Model" that consists of uncover ideas, accumulate ideas, share ideas, ensure ideas, and unfold ideas. According to the steps of the model, a web-based lessen plan on coordinates in middle school mathematics was developed. Finally, a web-based their divergent thinking, critical thinking and subject knowledge for mathematical problem solving creatively. Some sample web pages were suggested for developing a web site based on the purposed model and the lessen plan.

Fluorescence in situ Hybridizaton에 의한 염색체 이상의 절단점의 규명

구선회,송인숙 충남대학교 의과대학 지역사회의학연구소 1996 충남의대잡지 Vol.23 No.2

Fluorescence in situ hybrididzation(FISH) is a novel molecular genetic technique. It used for gene mapping in researches and in clinical cytogenetic fields. We perfomed FISH using various kinds of probes for the determination of breakpoints in chromosomal abnormalities. Following results were obtained: 1. Determination of breakpoint in complex translocations. FISH results with chromosome 14/22 centromere probe showed that the breakpoint was 14q11 in the patient with corpus callosum agenesis and complex transocation. 2. Determination of breakpoint of inv(16) in acute myelomonocytic leukemia. FISH results with inv(16) probe showed that the breakpoint was between 16pll and p13 in an abnormal inv(16) chromosome. 3. Determination of breakpoint of dup(17q) in acute myelocytic leukemia. FISH results with iso 17q probe showed that the breakpoint was 17q21 and the duplicated segment from q21-q25. 4. Determination of breakpoint of isodicentric X chromosome. FISH results of Turner syndrome with two iso Xq chromosome using a-satellite X centomere probe showed that the breakpoint was in proxim portion of centromere. From the above results, we could easily find out the breakpoints of chromosomal abnormalities by FISH. It was helpful for the tracing of pathogenesis of genetic disease and cancers.

미세절제술과 비교 유전자 보합법에 의한 각종 종양에서의 유전자 변화에 관한 연구

구선회,신소영,임춘화,전영미,이윤이,김진만 충남대학교 의과대학 지역사회의학연구소 2000 충남의대잡지 Vol.27 No.2

For the evaluation of oncogenesis, progression and prognosis of cancer, CGH is an important technique, because this technique is economic due to utilization of only one probe and lack of culture, screening mathod of whole genome and possibility of retrospective and prospective study. By the CGH, genornic variation of 20 breast cancer tissues, 23 stomach cancer tissues and 16 bladder cancer tissues were analyzed. The results were as followes ; 1. breast cancers The CGH results showed gains on chromosomes 8q(40%), lq(30%), 17q(15%), 20q(15%), 18q (15%), 5p(15%), and 13q(15%). The Deletions were on chromosomes 17p(45%) and 22q(20%). High-level amplifications(green/red ratio >1.5) were noted on chromosomes 1p31, iq, 3q25-qter, 5p, 7q31-qter, 8q, 9q22-qter, 10p, l1p, 11q22-qter, 12p, 12q24, 14g21-qter, 15q23-qter, 17q, 18p, 18q12-qter, 20p, and 20q. By comparison with infiltrating ductal carcinoma, the two medullary carcinomas showed high-level amplification on chromosomes iq3l, lq, 8q, 10p, 11p and 12p. 2. stomach cancers 1) Usual amplification sites of genome were lq, 13q, 17q, 20p,q. 2) 17p was the most common deletion site. The other sites of the deletion were lq, 4q. 3) In intestinal type of stomach cancer, genomic variation is more common than diffuse type. 4) In the cases of no evidence of lymph node metastasis, deletion of 17p is absent but amplification of 8q is obvious in the case of lymph node metastasis. 3. bladder cancers Common amplification of copy numbers of DNA sequences by CGH were seen at 1q, 3q, 4q, 5p, 6pq, 7p, 8q, 11q, 12q, 13q, 17q, 18q and 20pq(more than 20% of cases). High level amplification was noted at 1p32, 3p2l, 3q24, 4q26, 8q21-ter, 11q14-22, 12q15-21, 12q21-24, 13q 21-31, 17q22, and 18q22. Deletions were noted at 2q21-qter, 4q13-23, 5q, 8p12-22, 9pq, 11p13-15 (more than 20% of cases).

외국어 학습과 동기 : 연구의 동향 및 쟁점 A Review of the Issues

구광현,곽선연 안양대학교 1997 논문집 Vol.17 No.-

This paper addressed the issue of motivation in foreign language learning in five main categories. First of all, the importance of motivation in language learning was stressed with a vivid example which could overcome unfavorable circumstances in other aspects of language learning. Secondly, a layman's or 'naive' view of the problem was introduced, and general psychological viewpoints on motivation were reviewed. Thirdly, earlier and contemporary views of specific motivation for language study were reviewed. Particularly, the work of Robert Gardner was analysed in detail. Fourthly, Gardner's work was critically evaluated and the major issues in the study of motivation were discussed. Finally, several attempts to relate the findings on individual differences in motivation to a wider educational framework were discussed with regards to further studies.

모듈 설계 및 조립식 건축공법을 통한 학교 건물의 다양성 및 가변성을 위한 연구

이선구 崇實大學校 生産技術硏究所 1996 論文集 Vol.26 No.1

To meet the increasing demand of school building construction in regard with the variable adn flexible use of school floor plans the basic planning concepts of modular design method are studied. The modular design method in school building construction founded on optional statical span of 7.20-8.40 m has structural planning grid and grid for partitionand mechanical equipment for buildings as it's basic design components. Four design examples of school building with intensive use of modular design method are analysed in the final chapter of the study.

입모양 특징추출기반 감정인식시스템

백선경,김판구 조선대학교 전자정보통신연구소 2002 電子情報通信硏究所論文誌 Vol.5 No.2

In twenty-one century, these studies that add the human's feelings to computer system, so that make more close and natural interface between human and computer have progressed actively. In this study, we design a system that computer recognizes the human's emotion. The system is able to recognize human's feeling by extracting the features of the mouth shapes. It is based that the mouth shapes express the person's emotion well. Most of the previous studies suggested the method that recognize the most common feelings such as pleasure, sadness, surprise, anger, and so on. On the other hand, this study is based on more various 13 kinds of definite feelings. This system extracts the mouth shapes from input data, people's face images. Then, it extracts the feature values of the mouth shapes through the standard of the location and size of mouth. At this time, this system uses the DCT method. After that, we suggest the BP neural network to match the extracted feature values with the learned feelings in the end of this study. In this study, we are able to confirm that this system distinguishes each mouth shapes through the feature values and standard vector values.

급성 ST 분절 상승 심근경색증의 표준진료지침 설계

권선옥,김우식,오명기,나종천,이홍기,조욱현,최석구 인제대학교 2006 仁濟醫學 Vol.27 No.-

The use of critical pathways for a variety of clinical conditions has grown rapidly in recent years, particularly pathways for patients with acute myocardial infarction. We intend to determine the impact of a clinical pathway on ST-elevation myocardial infarction (STEMI) and to evaluate the efficacy and safety of facilitated percutaneous coronary intervention (PCI) compared with primary PCI. Low risk STEMI patients (ST elevation >0.1mV in more than 2 limb leads or ST elevation >0.2mV in contiguous precordial lead, chest pain lasting more than 30 min without response to nitroglycerin) will be included. All patients will be also treated medically according to critical pathway. STEMI is one of the common diseases in emergency medicine and so it is necessary to establish realistic treatment guidelines. The use of critical pathways will improve the quality of care.