Effective Microorganism Substance (EM-S) Reduces Development and Aggravation of Atopic Dermatitis-like Skin Lesions in NC/Nga Mice

Park, Kwang-Hyun,Jeong, Seung-Il,Mok, Ji-Ye,Cho, Jung-Keun,Park, Ji-Min,Jeon, In-Hwa,Kim, Hyeon-Soo,Jang, Seon-Il The Korean Society for Applied Biological Chemistr 2011 Applied Biological Chemistry (Appl Biol Chem) Vol.54 No.2

In a previous study, our group showed that the effective microorganism substance (EM-S) produced by fermentation of medicinal plants with effective microorganisms has an antiinflammatory effect on atopic dermatitis-like lesions in NC/Nga mice. However, the possible antiinflammatory effect and skin barrier function of EM-S for inflammatory cell infiltration, Interleukin-4 (IL-4) production, C-C chemokine receptor 10 (CCR10), and filaggrin (FLG) expression were not reported. Therefore, effects of EM-S on the development of atopic dermatitislike skin lesions in NC/Nga mice were evaluated. Efficacy of EM-S was judged by measurement of scratching behavior, T-cell subset infiltration, cytokine production, and FLG expression. Topical application of EM-S significantly reduced scratching behavior in NC/Nga mice caused by house dust mite antigen (Dermatophagoides farinse extract, DfE) sensitization. IL-4 production and $CD4^+$ and $CD45^+$ cell infiltrations were significantly reduced by EM-S. CCR10 expression was also significantly inhibited by EM-S. EM-S treatment also increased the level of FLG reduced by DfE sensitization. These results demonstrate EM-S, when applied topically, may be an effective substance for management of atopic dermatitis patients.

Isolation and Characterization of Antimicrobial Substance Macrolactin A Produced from Bacillus amyloliquefaciens CHO104 Isolated from Soil

( Seung Je Lee ),( Jeong Yong Cho ),( Jung Il Cho ),( Jae Hak Moon ),( Ki Deok Park ),( Young Ju Lee ),( Keun Hyung Park ) 한국미생물생명공학회 2004 Journal of microbiology and biotechnology Vol.14 No.3

아토피 피부염 모델에 대한 β-1,3/1,6-glucan과 Lactobacillus plantarum LM1004의 면역 조절 효과

김인성(In Sung Kim),김성학(Sung Hak Kim),김정아(Jeong A Kim),유다윤(Da Yoon Yu),김광일(Gwang Il Kim),박동찬(Dong-Chan Park),임종민(Jong Min Lim),이상석(Sang Suk Lee),최인순(In Soon Choi),조광근(Kwang Keun Cho) 한국생명과학회 2018 생명과학회지 Vol.28 No.1

본 연구에서는 아토피 피부염 동물 모델에 대한 β-1,3/1,6-glucan과 L. plantarum LM1004의 면역조절 효과를 확인하고자 하였다. 가려움증의 횟수와 유출된 evans blue, 그리고 혈청 IgE와 histamine의 농도는 β-1,3/1,6-glucan과 L. plantarum LM1004를 섭취한 그룹에서 아토피 피부염 유발그룹에 비해 유의적으로 감소하는 결과를 나타내었다. 아토피 피부염이 유발되면 전사 수준에서 Th2 및 Th17 세포의 전사인자 및 cytokine은 과발현되며, β-1,3/1,6-glucan과 L. plantarum LM1004를 섭취하였을 때 이를 유의적으로 감소되었다. 또한 β-1,3/1,6-glucan과 L. plantarum LM1004는 Th1 및 Treg 세포의 전사인자(T-bet, GATA-3, RORγT, Foxp3) 및 cytokine (INF-γ, IL-4, IL-17, TGF-β)의 발현을 증가시킴으로써 면역 균형을 조절하는 것으로 나타났다. Galectin-9과 filaggrin은 아토피 피부염 유발 처리군에서 유의적으로 가장 낮았으며, β-1,3/1,6-glucan 처리군에서 유의적으로 가장 높게 나타났다. 이와 반대로 TSLP는 아토피 피부염 유발그룹에서 유의적으로 가장 높았으며 β-1,3/1,6-glucan과 L. plantarum LM1004를 섭취한 그룹은 대조군과 유사한 수준이었다. 이러한 결과를 통해 β-1,3/1,6-glucan과 L. plantarum LM1004는 아토피 피부염 동물 모델에서 면역조절 작용 및 아토피 피부염의 개선 효과를 가짐을 알 수 있었다. 따라서 β-1,3/1,6-glucan과 L. plantarum LM1004는 아토피 피부염에 유용한 천연소재로서 사용될 것으로 기대된다. In this study, we examined the efficacy of the immune regulation of β-1,3/1,6-glucan and Lactobacillus plantarum LM1004 on atopic dermatitis models. The oral administration of β-1,3/1,6-glucan and L. plantarum LM1004 on mice significantly decreased the amount of scratching, leakage to evans blue, and concentrations of serum immunoglobulin E (IgE) and histamine compared with the atopic dermatitis–induced group. When atopic dermatitis was induced, the transcription factors (GATA-3, retinoic acid-related orphan receptor γ T [RORγT]) and cytokines (interleukin-4 [IL-4], IL-17) of Th2 and Th17 cells were overexpressed at the transcriptional level, and they significantly decreased with oral administration of β-1,3/1,6-glucan and L. plantarum LM1004. In addition, β-1,3/1,6-glucan and L. plantarum LM1004 were shown to modulate the immune balance by increasing the expression of Th1 and Treg transcription (T-bet, forkhead box p3 [Foxp3]) and cytokines (interferon-γ [IFN-γ], transforming growth factor-β [TGF-β]). Galectin-9 and filaggrin were significantly lower in the atopic dermatitis–induced group and significantly higher in the β-1,3/1,6-glucan-treated group. In contrast, thymic stromal lymphopoietin (TSLP) was highest in the atopic dermatitis–induced group, while mice that were orally administered β-1,3/1,6-glucan and L. plantarum LM1004 showed similar TSLP levels to the control group. These results indicate that β-1,3/1,6-glucan and L. plantarum LM1004 have immunomodulatory effects and atopic dermatitis improvement effects in an animal model of atopic dermatitis. Therefore, it is expected that β-1,3/1,6-glucan and L. plantarum LM1004 can be used as natural materials in the treatment of atopic dermatitis.

Adiponectin is a negative regulator of NK cell cytotoxicity.

Kim, Kun-Yong,Kim, Jae Kwang,Han, Seung Hyun,Lim, Jong-Seok,Kim, Keun Il,Cho, Dae Ho,Lee, Myeong-Sok,Lee, Jeong-Hyung,Yoon, Do-Young,Yoon, Suk Ran,Chung, Jin Woong,Choi, Inpyo,Kim, Eunjoon,Yang, Young American Association of Immunologists 2006 Journal of Immunology Vol.176 No.10

<P>NK cells are a key component of innate immune systems, and their activity is regulated by cytokines and hormones. Adiponectin, which is secreted from white adipose tissues, plays important roles in various diseases, including hypertension, cardiovascular diseases, inflammatory disorders, and cancer. In this study the effect of adiponectin on NK cell activity was investigated. Adiponectin was found to suppress the IL-2-enhanced cytotoxic activity of NK cells without affecting basal NK cell cytotoxicity and to inhibit IL-2-induced NF-kappaB activation via activation of the AMP-activated protein kinase, indicating that it suppresses IL-2-enhanced NK cell cytotoxicity through the AMP-activated protein kinase-mediated inhibition of NF-kappaB activation. IFN-gamma enhances NK cell cytotoxicity by causing an increase in the levels of expression of TRAIL and Fas ligand. The production of IFN-gamma, one of the NF-kappaB target genes in NK cells, was also found to be suppressed by adiponectin, accompanied by the subsequent down-regulation of IFN-gamma-inducible TRAIL and Fas ligand expression. These results clearly demonstrate that adiponectin is a potent negative regulator of IL-2-induced NK cell activation and thus may act as an in vivo regulator of anti-inflammatory functions.</P>

마우스 복강대식세포에서 가감공진단(加減拱辰丹)의 항염증 효과

김홍준,김영식,목지예,정승일,황성연,조정근,장선일,Kim, Hong-Jun,Kim, Young-Sik,Mok, Ji-Ye,Jeong, Seung-Il,Hwang, Sung-Yeoun,Cho, Jung-Keun,Jang, Seon-Il 대한한의학방제학회 2011 大韓韓醫學方劑學會誌 Vol.19 No.1

Objectives : In a previous study, we have shown that Gagam-Gongjin-Dan(GGD) has an inhibitory effect on the ovalbumin-induced immune responses and a hepatoprotective effect on actaminophen-induced liver injury in Balb/c Mice. However, the possible anti-inflammatory effect of GGD extract for inflammatory mediators was not reported. Therefore, the purpose of this study was to investigate an inhibitory effects of GGD extract against lipopolysaccharides(LPS) induced inflammatory mediators in mouse peritoneal macrophages. Methods : GGD extract was prepared by extracting with methanol for 7 days. The extract was freeze-dried following filtration through vacuum distillation system. Accumulated nitrite, an oxidative product of nitric oxide(NO), was measured in the culture medium by the Griess reaction. The levels of prostaglandin $E_2(PGE_2)$, interleukin-$1{\beta}$(IL-$1{\beta}$), tumor necrosis factor-${\alpha}$(TNF-${\alpha}$) were measured by enzyme-linked immunosorbent assay. The expressions of inducible nitric oxide synthase (iNOS) and cyclooxygenase-2(COX-2) were measured by Western blot analysis. Results : GGD extract (50-$400\;{\mu}g$/ml) per se had no cytotoxic effect in LPS-stimulated peritoneal macrophages. GGD extract dose-dependently reduced NO, $PGE_2$, IL-$1{\beta}$ and TNF-${\alpha}$ production and COX-2 activity caused by stimulation of LPS. The levels of iNOS and COX-2 protein expressions were markedly suppressed by the treatment with GGD extract in a dose dependent manner. Conclusions : These results suggest that GGD extract has an anti-inflammatory effect against LPS-induced inflammatory mediators in peritoneal macrophages, these properties may contribute to inflammation disease care.

천연항균성 물질을 이용한 Vibrio vulnificus의 살균 및 독소생성 억제효과

조성환,서일원,최종덕,전상수,라택균,정수근,강동훈 國立統營水産專門大學 附設 水産科學硏究所 1993 수산과학연구소보고 Vol.4 No.-

생선회용 해산물에 오염되어, 생식한 경우 발생되는 패혈병의 원인균주인 Vibrio vulnificus의 생육 및 독성생성을 억제할 목적으로 천연 항균제인 Grapefruit종자추출물(GPSE)을 이용하여 그 항균효과를 검토하였다. In vitro 시험결과, Vibrio vulnificus에 대한 GFSE의 생육최소저해농도는 50~100 ppm 정도이었으며, Vibrio vulnificus 균체세포틀 100 ppm 농도의 GFSE 용액으로 처리하고 전자현미경 촬영시료로 조제하여 촬영한 후, 미생물형태을 무처리대조구와 비교, 검토한 결과, GFSE 처리로 세포막 기능이 파괴되어 세포내용물이 균체외부로 유출되어 균체의 생육이 억제되고 균체내부가 빈 ghost 형태의 균체수가 증대되어 GFSE의 항균효과를 확인할 수 있었다. 한편, 생선회용 해산물에 GFSE를 근육주사하고 Vibrio vulnificus를 접종한 수조내에서 사육하여 채취한 어육부를 마쇄하여 일정량씩을 표준사료에 첨가해서 통닭용 병아리를 실험동물로 하여 사양실험을 실시한 결과. 체중증가율, 단백질이용효율, 혈청내 효소활성, 혈청의 주요성분함량 등에 있어서, GFSE 처리수조내에서 사육한 해산물을 첨가한 시료시험구의 경우, 무첨가사료처리구와 비교할 때 뚜렷한 차이를 목격할 수 없었으며, GFSE 첨가에 따른 실험동물의 성장저해 또는 독성현상을 유발하지 않음을 알 수 있었다. 아울러, 각 처리구에서 사양한 해산물의 어육부에 오염된 Vibrio vulnificus의 균수를 측정한 결과, 250ppm 이상의 GFSE 처리구에서는 Vibrio vulnificus를 전혀 검출할 수 없었다. To prevent food-poisoning outbreaks by Vibrio vulnificus the antimicrobial efficacy of grapefruit seed extract (GFSE) was examined. Minimal inhibitory concentration (in vitro) for the microorganism was found to be 50~100 ppm. Transmission electron micrographs of Vibrio vulnificus showed the biocidal action of this natural antimicrobial agent would be related to specific respiratory effect coupled with the destruction of permeable function of microbial cell membrane. After Anguilla japonia GFSE-injected to the body was incubated in the seawater contaminated by Vibrio vulnficus the fish meats were taken up, mixed with control diet and used as a diet in the feeding experiment. In this experiment the effect of GFSE treated with fish muscle on body weight protein efficiency ratio, serum enzymes and serum blood component of broiler chicks was investigated. It is proved from this study that there is neither Vibriosis nor toxicity associated with GFSE itself and fish meats treated with it when it is injected to the fish body at a level of 250 ppm or less.

좌새골하정맥을 통하여 중심정맥도관 삽입 3 일 후에 발생한 상대정맥천공과 우흉수 : 증례 보고 A case report

조형찬,손주태,최준영,신일우,이헌근,정영균 대한마취과학회 2002 Korean Journal of Anesthesiology Vol.43 No.4

A 67-year-old male patient had coronary artery bypass graft. Before the induction of general anesthesia, 16 gauge central venous catheter was inserted via left subclavian vein. Initial chest anteroposterior (AP) radiograph showed adequate position, and the central venous pressure measurement through it was satisfactory. This patient's progress was satisfactory, and he was extubated on the second postoperative day. Daily chest AP radiograph had been unremarkable until the third postoperative day, when a right-sided pleural effusion was seen. A day later, the patient complained severe chest pain and dyspnea. We suspected perforation of superior vena cava wall by tip of central venous catheter on the chest computerized tomograph (CT). The injection of radiopaque dye through the central venous catheter confirmed spillage into the mediastinum on the chest CT. Right chest tube was inserted. The patient's condition dramatically improved after chest tube insertion. (Korean J Anesthesiol 2002; 43: 525~530)

표준 용매 벤젠의 Rayleigh 비의 온도 의존성

박일현,이종근,윤관한,조동환,민병길,방대석 金烏工科大學校 2000 論文集 Vol.21 No.-

The Rayleigh ratio of benzene is a very important physical constant with which the integrated scattered intensity of laser light scattering can be converted into the absolute value. In this study temperature dependence of Rayleigh ratio of this solvent has been investigated with two different methods in the temperature range of 25-75℃. It was found that in the direct measurement the non-linear behavior of its Rayleigh ratio were observed as increasing temperature even if the scattering volume correction was applied to the scattered intensity of benzene. However the result of indirect measurement based upon the invariance of molecular weight of standard polystyrene polymer for any polymer solution temperature showed us that its temperature dependence up to 75℃ could be expressed with the Benoit's linear equation rather than the non-linear one. It seems that the scattered intensity in direct method has more complicate contributions at the elevated temperature. Further research works are necessary in order to solve such discrepancy clearly.

P-Toluenesulfonanilide의 공간군 결정

오인근,김창진,서일환,조성일 충남대학교 1985 忠南科學硏究誌 Vol.12 No.2

The space group os p-toluenesulfonanlide, C_13 H_13 NO_2S, has been determined by single crystal X-ray photographic data collected three-dimensionally using Weissenberg and precession methods with Cu Kα radiation (λ=1.5418 Å). The crystal data are monoclinic, space group p 1 2_1/n 1 with cell dimensions a=15.369±0.423Å, b=9.806±0.009Å, c=8.99±0.03Å, β=98.53±0.18°and z=4. The precise a and b parameters were obtained by finding a proper extrapolation function and using the least-squares refinement of eight 2θ values measured on (hk0) and (h0ℓ) Weissenberg photographs, and because of insufficient number of diffraction-spots along the c^* axis the c parameter and the β angle were measured from oscillation and Weissenberg photographs respectively. Four molecules in a unit cell appear to be related by screw diads parallel to the b axis and n-glide planes perpendicular to it.

신물류시대에 대응하기 위한 원예농산물의 산지유통 효율화를 위한 과제

성진근,조일호 충북대학교 첨단원예기술개발연구센터 1999 연구보고서 Vol.4 No.-

'90년대 후반기 이후 급진전되고 있는 신물류유통질서의 핵심은 낮은 농산물유통원가의 실현이고, 이를 위해서는 상품구입가격의 절감과 유통방식의 변경, 그리고 물류비용의 절감이 뒷받침되어야한다. 산지의 농산물출하조직(농협, 영농조합법인, 포장센터등)들은 신물류질서에 적응하기 위하여 포장, 규격화, 등급화, 팔레트단위화, 정보화를 적극적으로 실현해야한다. 나아가서 경제적인 출하단위를 이룰 수 있는 지역생산력의 규모화와 생산 이후의 수확, 선별등 상품화과정에서 소비자의 욕구를 충족시킬 수 있는 기획력과 경영능력, 나아가서 생산자의 거래교섭력을 높일 수 있는 마켓팅기법도 아울러 갖추어 나가야한다. 정부는 기존의 당위적인 「제도적 접근」방식에서 벗어나서 시장에서 일어나고 있는 변화를 분석하여 경쟁원리에 입각하여 시장화를 촉진시키는 역할에 충실해야 한다. 이 과정에서 농산물가격과 거래의 안정화를 통한 사회적 후생의 극대화가 정책의 우선적인 목표가 되어야 한다. 그러나 영세한 다수의 생산자로 구성되어 있는 산지출하조직들의 시장적응력을 육성하고 거래교섭력을 보완해주는 시책이 지속적으로 뒷받침되어야만 산지유통이 소비지유통의 발전을 조화롭게 뒷받침하면서 신물류시대에 적응하여 발전해나갈 수 있을 것이다.