대장선암에서 p53 과 P-glycoprotein의 분포에 관한 연구

강상균,김의한,장인성,김대중,양승하 순천향의학연구소 1997 Journal of Soonchunhyang Medical Science Vol.3 No.1

Adenocarcinoma of colon and rectum express many oncogenes. The p53 acts as tumor suppressor in normal tissue but mutant forms are known as doing important role in carcinogenesis of many malignant tumors, such as colorectal cancer, gastric cancer, and breast cancer. P-glycoprotein shows distribution in many normal tissues and malignant tumor. This protein occurs multidrug resistance to therapeutic anticancer drug, resulting in poor prognosis of patients. The expression of p53 and P-glycoprotein has been studied by many authors according to clinicopthologic findings of colorectal adenocarcinoma, but these results are controversial yet. To observe the relationship between both antigen and pathologic findings, including prognostic factos, of colorectal adenocarcinoma, we analysed 13 cases adenoma and 62 cases adenocarcinoma then processed for immunohistochemical stain by SLAB method using mouse monoclonal anti-p53 and JSB-1. The results were as followings; 1) The expression rate of p53 in adenoma and adenocarcinoma are 38.5% and 71.0%, respectively, and P-glycoprotein are 46.6% and 58.1%, respectively. 2) The p53 revealed higher expression rate in case of well differnetiated form, invasion to serosa and surrounding adipose tissue, lymphatic metastasis compared to that of moderately and poorly differentiated form, invasion to muscle, no lymphatic metastasis, but no difference accoding to site. 3) P-glycoprotein revealed higher positive rate in case of right side, invasion to surrounding adipose tissue, lymphatic metastasis compared to that of left side, invasion to muscle and serosa, no lymphatic metastasis, but no difference according to differentiation. 4) Both of p53 and P-glycoprotein revealed higher ex pression in case of right side, well and moderately differentiated form, invation to serosa and surrounding adipose tissue, lymphatic metastasis compared to that of left side, poorly differentiated form, invasion to muscle, no lymphatic metastasis. Above results revealed that the expression of p53 begins in adenoma and shows close relation with development of colorectal carcinoma. p53 and P-glycoprotein may reveal the relation with poor prognosis. Adenocarcinoma showing both positive reaction of p53 and P-glycoprotein is also similar prognostic relation.

Uncoupling Protein 3의 골격근 세포내 과발현이 OLETF 백서 및 배양된 골격근 세포에서 포도당대사에 미치는 영향

한정희,박혜선,고정민,김하영,강호경,이인규,박중열,홍성관,이재담,이기업 대한당뇨병학회 2002 Diabetes and Metabolism Journal Vol.25 No.6

연구배경:Uncoupling protein(UCP)는 미토콘드리아의 내막에 위치하는 단백질로 세포내의 과다한 에너지를 열로 발산시키는 기능을 가진다. 최근 동물의 갈색지방조직에만 존재하는 UCP와 유사성을 가진 아형들(UCP2,3)이 사람에게도 존재함이 알려져 큰 관심을 끌도 있는데 이중 UCP3는 그 발현이 골격근세포와 갈색지방조직에만 국한된다. 본 연구에서는 UCP3가 체내 인슐린 감수성을 결정하는데 가장 중요한 조직인 골격근에 국한되어 발현되는 점에 착안하여 UCP3를 골격근세포에 과발현시켰을 때 포도당 대사에 어떠한 영향이 나타나는 지를 조사하였다. 방법:25주령의 8마리의 OLETF 백서를 대상으로 하여 4마리는 골격근에 adenovirus 2mL(1×10¹²pfu/mL)를 주사하여 대조군으로 하였고 4마리는 골격근에 재조합법으로 제작된 adenovirus­UCP3 2mL(1×10¹²pfu/mL)를 주사하였다(UCP3 과발현군). UCP3를 투여한 백서에서 먹이섭취가 증가하는 경향이 있어 그 전날 대조군이 먹은 야의 먹이만큼 투여하였다. 골격근에 adenovirus를 주사한 10일 후에 euglycemic hyperinsulinemic clamp를 시행하였다. Adenovirus­UCP를 C2C12 골격근 세포에 transfection시켜 UCP3를 C2C12 골격근 세포에 transfection시켜 UPS3­C2C12를 만들고 C2C12 골격근 세포와 UPS3­C2C12 골격근 세포에서 포도당 수송 및 당원합성을 측정하였다. 결과:UCP3 과발현 OLETF에서 체중이 감소하는 경향을 보였고 인슐린 감수성이 증가하였다. C2C12세포에서 기저상태 포도당 수송은 1.28±0.17μmol/L/min였고 100nM 인슐린으로 2시간 처리한 후 2.67±0.20 μmol/L/min로 증가하였다. UCP3­C2C12 세포에서는 기저상태 포도당 수송이 3.98±0.13μmol/L/min로 증가되었고 인슐린 처리 후 5.74±0.44μmol/L/min로 증가하였다. 인슐린을 처리한 UCP3­C2C12 세포에 P13K 억제제인 wortmannin을 첨가하였을 때 포도당 수송활성이 3.81±0.20μmol/L/min로 감소하였다. 기저상태 당원합성은 C2C12 세포에서 0.25±0.01μmol/L/min였고 인슐린 처리 후 0.45±0.01μmol/L/min로 증가하였다. UCP3­C2C12 세포에서는 기저상태 당원합성이 0.62±0.01μmol/L/min였고 인슐린 처리 후 1.26±454μmol/L/min로 증가하였다. UCP3­C2C12세포에 wortmannin을 첨가하였을 때 당원합성율이 0.80±0.04μmol/L/min로 감소하였다. 결론:UCP3 과발현이 OLETF 백서에서 인슐린 감수성을 증가시켰고 골격근세포에서 포도당 수송 및 당원합성을 증가시켰다. wortmannin을 첨가하였을 때 포도당 수송 및 당원합성이 감소함으로 보아 이 과정이 인슐린 신호전달체계인 P13K에 일부 의존함을 알 수 있었다. Background : UC P3 is a mitochondrial membrane protein expressed selectively in the skeletal muscle and brown adipose tissue. Since the skeletal muscle is the main organ determining insulin sensitivity in the body, it was hypothesized that UCP3 overexpression in skeletal muscle cells would improve glucose metabolism. Methods : An adenovirus-UCP3 was produced by a recombinant DNA method. OLETF rats were divided into 2 groups. Four rats were injected with the adenovirus-UCP3 (UCP3 group) and others were injected with the adenovirus(control group) in the skeletal muscle. The UCP3 group was provided with the same quantity of food as that consumed by the control group on the previous day. Insulin sensitivity was evaluated by the euglycemic hyperinsulinemic clamp method. In a separate experiment, glucose transport and glycogen synthesis we evaluated in C2C212 cells transfected with ether an adenovirus or the adenovirus-UCP3. Results : The insulin sensitivity improved significantly and the body weight decreased in the UCP3 group. The glucose transport and glycogen synthesis were higher in the UCP3-C2C12 skeletal muscle cells at the basal state. After insulin treatment, glucose transport and glycogen synthesis were also higher in the UCP3-C2C12 cells but the increments were reduced after treatment with wortmannin, a PI3K inhibitor. Conclusion : Insulin sensitivity was higher in the UCP3-overexpressed OLETF rats in the in vivo study. UCP3 transfection also increased glucose transport and glycogen synthesis in the cultured skeletal muscle cells by a PI3K dependent mechanism(J Kor Diabetes Asso 25 :460~468, 2001).

2-Bromoacetylnaphthalene을 螢光誘導體化劑로 利用한 Alclofenac의 HPLC 分析에 관한 硏究

李允中,曺正吉,河仁植,金容熙 成均館大學校 科學技術硏究所 1990 論文集 Vol.41 No.1

A simple, rapid and high sensitive method for determination of alclofenac is described. 2-Bromoacetylnaphthalene was used as the pre-column fluorescent derivatizing reagent for high performance liquid chromatography. Alclofenac was derivatized quantitatively into fluorescent compound by reacting with 2-bromoacetylnaphthalene in the presence of 18-crown-6 ether in acetonitrile. The optimum conditions for the derivatization such as concentration of KOH, 18-crown-6 and 2-bromoacetylnaphthalene, reaction temperature and reaction time were investigated. The structure of alcolofenac derivative was confirmed from IR, NMR and mass spectra. The fluorescence properties of alclofenac derivative were examined. The derivative was separated on a reverse phase column (Lichrosorb RP-8) in isocratid elution mode using the secondary mixture of acetonitrile and water as the mobile phase. The effluent was monitored by fluorometer.(Ex. 303, Em. 418 nm). The calibration plots for the peak area versus concentration of alclofenac observed to be linear(r =0.999).

Polyethylene glycol 용액(Colyte^(R))을 복용한 후 발생한 Boerhaave 증후군 1예

이관행,기승석,김인숙,김민정,박인호,장세중,하지수,김응수 朝鮮大學校 附設 醫學硏究所 2006 The Medical Journal of Chosun University Vol.31 No.3

Spontaneous esophageal rupture (Boerhaave's syndrome) is a rare case that require early diagnosis and treatment because of its high mortality. The oral administration of osmotically balanced polyethylene-glycol-based electrolyte preparation is a commonly prescribed preparation for cleansing in patients undergoing colonoscopy. Nausea, vomiting and abdominal pain are frequently seen, but serious adverse reactions are rare. Esophageal rupture secondary to severe vomiting, which occurred during colonoscopy preparation using polyethylene gIycoI(PEG) electrolyte solution is extremely rare and only few cases have been published in the literature. We report a case of spontaneous esophageal rupture after routine administration of the PEG before colonoscopy. 대장 정결을목적으로 PEG 사용 후 아주 드물게 발생하는 Boerhaave 증후군 1예를 경험하였기에 보고하는 바이다.

랫드에서 단일시험법을 이용한 반하 물추출물의 생식독성시험

이기남,유욱준,백인정,연정민,남상섭,이범준,남상윤,김윤배,강현규,안병우,김대중,황방연,노재섭,홍정섭,성하정,김순선,조대현,윤영원 충북대학교 동물의학연구소 2006 Journal of Biomedical and Translational Research Vol.7 No.1

본문참조

Pulmonary Adenocarcinoma in a Dairy Goat Raised in Korea

Ha-Hyun Kim,In-Jin Hwang,Su-Jin Park,Sang-Ik Park,Mun-Il Kang,Bang-Hun Hyun,In-Joong Kim,Dong-Kun Yang,Chang-Hee Kwon,Kyoung-Oh Cho 한국실험동물학회 2007 Laboratory Animal Research Vol.23 No.4

Pulmonary adenocarcinoma was found in a 3-year-old dairy goat raised in Korea. At necropsy, a firm, greyish round neoplastic mass measuring approximately 1 ㎝ in diameter was detected in the left diaphragmatic lung lobe. Histologically, tumor lesions consisted of acini and papilla lined by short columnar and cuboidal epithelial cells. The stroma of tumors was thin and had mild lymphoid cell infiltration. Neutrophils and necrotic cell debri and/or mucus were densely compacted in the tumor acini. There were lymphoid cell aggregations in and around the tumor lesions. Mitotic figures were not detectable in the tumor lesions. No metastasis of this tumor was observed in any organs and tissues. From these results, this tumor was diagnosed as well differentiated adenocarcinoma in a dairy goat. This is the first report about pulmonary adenocarcinoma in goats raised in Korea. Since lung adenocarcinomas found in sheep and goats are mostly caused by betaretrovirus, the possibility of a retroviral etiology of this tumor will be evaluated in this article.

양식 담수어 및 해산어의 사료 Carotenoids 대사의 비교와 체색개선에 미치는 영향

하봉석(Bong-Seuk Ha),권문정(Moon-Jeong Kweon),박미연(Mi-Yeon Park),백승한(Sung-Han Baek),김수영(Soo-Young Kim),백인옥(In-Ok Baek),강석중(Seok-Joong Kang) 한국식품영양과학회 1997 한국식품영양과학회지 Vol.26 No.2

무지개 송어, 산천어, 뱀장어, 볼락 및 우럭에 대한 사료 carotenoids의 체내대사와 체색 개선효과를 검토하기 위하여, 사료에 β-carotene, lutein, canthaxanthin, astaxanthin 및 β-apo-8’-carotenal을 각각 첨가하여 4 내지 5주간 사육하여 표피의 carotenoids 성분의 변화를 분석, 비교한 결과는 다음과 같다. 무지개 송어 표피의 carotenoids 조성은, zeaxanthin, β-carotene 및 canthaxanthin이 주성분이였으며, 그 외 lutein, isocryptoxanthin 및 salmoxanthin을 소량 성분으로 함유하며, 표피의 carotenoids 축적율은 canthaxanthin 첨가구에서 높게 나타나 체색 선명화 효과가 가장 컸으며, astaxanthin, β-carotene 첨가구의 순으로 나타났다. 무지개 송어 표피에서의 carotenoids 대사경로는, β-carotene이 isocryptoxanthin, echinenone 및 canthaxanthin을 경유하여 astaxanthin으로 lutein은 canthaxanthin으로 산화되고, canthaxanthin은 isozeaxanthin을 경유하여 β-carotene으로 환원되며 astaxanthin은 triol을 경유하여 zeaxanthin으로 환원되는 대사경로를 추정할 수 있었다. 산천어 표피의 carotenoids 조성은, zeaxanthin이 주성분이며, 그 외 triol, lutein, tunaxanthin, β-carotene, β-cryptoxanthin 및 canthaxanthin을 소량 성분으로 함유하며, 표피의 carotenoids 축적율은 canthaxanthin 첨가구에서 높게 나타나 체색 선명화 효과가 가장 컸으며, lutein, β-carotene 첨가구의 순으로 나타났다. 산천어 표피에서의 carotenoids 대사경로는, β-carotene이 zeaxanthin으로 산화되고, lutein은 tunaxanthin을 경유하여 zeaxanthin으로 환원되고, canthaxanthin은 β-carotene을 경유하여 zeaxanthin으로 산화되며 astaxanthin은 triol을 경유하여 zeaxanthin으로 환원되는 대사경로를 추정할 수 있었다. 뱀장어 표피의 carotenoids 조성은, β-carotene이 주성분이였으며, 그 외 lutein, zeaxanthin 및 β-cryptoxanthin등이 소량 성분으로 함유하며, 표피의 carotenoids 축적율은 lutein 첨가구에서 높게 나타나 체색 선명화 효과가 가장 컸었고, canthaxanthin astaxanthin 첨가구의 순으로 나타났다. 뱀장어 표피에서의 carotenoids 대사경로는, β-carotene과 lutein은 그대로 축적되며, canthaxanthin은 β-carotene으로 그리고 astaxanthin은 zeaxanthin으로 환원되는 대사경로를 추정 할 수 있었다. 볼락 표피의 carotenoids 조성은, zeaxanthin, β-carotene, tunaxanthin A, tunaxanthin B, tunaxanthin C 및 lutein이 주성분이였으며, 그 외 β-cryptoxanthin, α-cryptoxanthin, astaxanthin을 소량 성분으로 함유하며, 표피의 carotenoids 축적율은 lutein 첨가구에서 높게 나타나 체색 선명화 효과가 가장 컸으며, β-carotene, canthaxanthin 첨가구의 순으로 나타났다. 볼락 표피에서의 carotenoids의 대사경로는, β-carotene은 lutein으로 산화되며, lutein, canthaxanthin astaxanthin 및 β-apo-8'-carotenal은 zeaxanthin을 경유하여 tunaxanthin으로 각각 산화 및 환원되는 대사경로를 추정할 수 있었다. 우럭 표피의 carotenoids 조성은, β-carotene, astaxanthin 및 zeaxanthin이 주성분이였으며, 그 외 α-cryptoxanthin, β-cryptoxanthin, lutein 및 canthaxanthin을 소량 성분으로 함유하며, 표피의 carotenoids 축적율은 lutein 첨가구에서 높게 나타나 체색 선명화 효과가 가장 컸으며, canthaxanthin, β-carotene 첨가구의 순으로 나타났다. 우럭 표피에서의 carotenoids 대사경로는 β-carotene은 β-cryptoxanthin으로 산화되고, lutein은 α-cryptoxanthin을 경유하여 β-carotene으로 환원되고, canthaxanthin은 β-cryptoxanthin, zeaxanthin을 경유하여 α-cryptoxanthin으로 환원되며, astaxanthin은 isocryptoxanthin, zeaxanthin을 경유하여 tunaxanthin으로 대사되며, β-apo-8’-carotenal은 β-cryptoxanthin, zeaxanthin을 경유하여 α-cryptoxanthin으로 환원되는 대사경로를 추정할 수 있었다. Effects of dietary carotenoids were investigated on the metaboβsm and body pigmentation of rainbow trout(Salmo gairdneri), masu salmon(Oncorhynchus macrostomos), eel(Anguilla japonica), rock fish(Sebastes inermis) and black rock fish(Sebastes schlegeβ). Three weeks later after depletion, these fishes were fed diet supplemented with β-carotene, lutein, canthaxanthin', astaxanthin or β-apo-8'-carotena1 for 4 to 5 weeks, respectively. Carotenoids distributed to and changed in integument were analyzed. In the integument of rainbow trout. zeaxanthin, β-carotene and canthaxanthin were found to be the major carotenoids, while lutein, isocryptoxanthin and salmoxanthin were the minor carotenoids. In the integument of masu salmon, zeaxanthin was found to be the major carotenoids, while triol, lutein, tunaxanthin, β-carotene, β-cryptoxanthin and canthaxanthin were the minor carotenoids. In the integument of eel, β-carotene was found to be the major carotenoids, while lutein, zeaxanthin and β-cryptoxanthin were the minor carotenoids. In the integument of rock fish, zeaxanthin, β-carotene, tunaxanthin(A~C) and lutein were found to be the major carotenoids, while β-cryptoxanthin, α-cryptoxanthin and astaxanthin were the minor carotenoids. Likely in the integument of black rock fish, β-carotene, astaxanthin and zeaxanthin were found to be the major carotenoids, whereas α-cryptoxanthin, β-cryptoxanthin, lutein and canthaxanthin were the minor contributor. The efficacy of body pigmentation by the accumulation of carotenoids in the integument of rainbow trout and masu salmon were the most effectively shown in the canthaxanthin group and of eel, rock fish and black rock fish were the most effectively shown in the lutein group. Based on these results in the integument of each fish, dietary carotenoids were presumably biotransformed via oxidative and reductive pathways. In the rainbow trout, β-carotene was oxidized to astaxanthin via successively isocryptoxanthin, echinenone and canthaxanthin. Lutein was oxidized to canthaxanthin. Canthaxanthin was reduced to β-carotene via isozeaxanthin, and astaxanthin was reduced to zeaxanthin via triol. In the masu salmon, β-carotene was oxidized to zeaxanthin. Lutein was reduced to zeaxanthin via tunaxanthin. Canthaxanthin was reduced to zeaxanthin via β-carotene. and astaxanthin was reduced to zeaxanthin via triol. In the eel, β-carotene and lutein were directly deposited but canthaxanthin was reduced to β-carotene, and cholesterol lowering effect by Meju supplementation might be resulted from the modulation of fecal axanthin, astaxanthin and β-apo-8'-carotenal were oxidized and reduced to tunaxanthin via zeaxanthin. In the black roch fish, β-carotene was oxidized to β-cryptoxanthin. Lutein was reduced to β-carotene via α-cryptoxanthin. Canthaxanthin was reduced to α-cryptoxanthin via successively β-cryptoxanthin and zeaxanthin. Astaxanthin converted to tunaxanthin via isocryptoxanthin and zeaxanthin, and β-apo-8'-carotenal was reduced to α-cryptoxanthin via β-cryptoxanthin and zeaxanthin.

Continuing evolution and interspecies transmission of influenza viruses in live bird markets in Korea.

Lee, Hyun-Jeong,Kwon, Ji-Sun,Lee, Dong-Hun,Lee, Yu-Na,Youn, Ha-Na,Lee, Youn-Jeong,Kim, Min-Chul,Jeong, Ok-Mi,Kang, Hyun-Mi,Kwon, Jun-Hun,Lee, Joong-Bok,Park, Seung-Yong,Choi, In-Soo,Song, Chang-Seon American Association of Avian Pathologists [etc.] 2010 Avian diseases Vol.54 No.1

<P>Live bird markets (LBMs) provide an ideal environment for the evolution and interspecies transfer of avian influenza viruses (AIVs). In this study, we analyzed AIVs present in LBMs in Korea during the winter seasons of 2006-08. Sixty-five AIVs that belong to four hemagglutination (HA) subtypes ofAIV (H3, H4, H6, and H9) were isolated from 644 pooled tissue or swab samples collected in LBMs. Most H9 subtypes of AIVs were isolated from Galliformes (chickens, silky fowls, pheasants, and guinea fowls), and other subtypes were isolated from Anseriformes (Pekin ducks and mallards). In addition, we obtained a single H3N2 virus from nasal swabs of dogs sold in LBMs, and the virus was genetically identical to the canine influenza virus (CIV) isolated from pet dogs in Korea. Phylogenetic analysis suggests that the Korean H9N2 viruses prevalent in chickens have provided their gene segments to AIVs circulating in ducks. These gene transfers facilitated reassortment events among AIVs and likely generated the ancestors of CIV in Korea. An animal challenge study using chickens, quail, mice, and dogs had shown that the H4 and H6 subtypes could replicate in mice and that some H4 and H6 viruses could replicate in chickens without preadaptation. In addition, two H3 subtype viruses (H3N2 and H3N8) induced interstitial pneumonia that accompanied clinical signs and seroconversion in dogs. Our findings indicate that the newly evolved AIVs have been continuously generated by reassortment in ducks, and these reassortments could result in expanding the host range of AIVs.</P>

Pathological Study on Interstitial Pneumonia in Korean Native Goats

Ha-Hyun Kim,In-Jin Hwang,Su-Jin Park,Sang-Ik Park,Young-Ju Jeong,Mun-Il Kang,Bang-Hun Hyun,In-Joong Kim,Dong-Kun Yang,Chang-Hee Kwon,Kyoung-Oh Cho 한국실험동물학회 2007 Laboratory Animal Research Vol.23 No.4

The interstitial pneumonia of animal and human can be induced by various factors throughout the aerogenous and hematogenous routes. Although the causative agents of the interstitial pneumonia in the sheep and goats are known to be usually infectious agents, its prevalence in the Korean native goats is not well studied in South Korea. Therefore, pathological examination of interstitial pneumonia whose causative agents were unknown was performed with four neonatal goats obtained from four farms. These goats showed progressive depression and anorexia. At necropsy, diffuse depression of all lung lobules with some patch normal lobules were observed. On the cut surface, visible exudates were not observed in airways except for one case who had mild foamy materials in the bronchioles and bronchi. The pulmonary parenchyma had a meaty appearance. Histologically, alveolar walls were thickened by prominent hyperplasia of type Ⅱ pneumocytes with some macrophages. The lining epithelia of the bronchioles and bronchi were intact. From these results, these cases were diagnosed as interstitial pneumonia in the Korean native goats. Since many infectious agents cause interstitial pneumonia with the infiltration of lymphoid cells into alveolar wall and peribronchial regions, the findings observed in the present cases suggest the possibility that these pulmonary lesions may not be caused by infectious agents. Therefore, further studies will need to find out the precise causative agents from the lung lesions in the affected goats.

Pathological Study on Purulent Pneumonia in Korean Native Goats

Ha-Hyun Kim,In-Jin Hwang,Su-Jin Park,Sang-Ik Park,Young-Ju Jeong,Mun-Il Kang,Bang-Hun Hyun,In-Joong Kim,Dong-Kun Yang,Chang-Hee Kwon,Bong-Joo Lee,Kyoung-Oh Cho 한국실험동물학회 2007 Laboratory Animal Research Vol.23 No.4

Bacteria belonging to the family Pasteurellaceae cause a common and economically important pneumonia to ruminants, particularly in temperate climates. However, bacterial pneumonia in the Korean native goats are not well studied in South Korea. Therefore, pathological examination of bacterial pneumonia was performed with two young goats obtained from two farms. These goats showed progressive dyspnea, depression and anorexia. At necropsy, cranioventral lobes which attached to pleural wall by fibrin were patch purple-red or white, and hard. Histopathologically, alveoli and small bronchioles were filled with variable proportions of necrotic neutrophils, fibrin, edema fluid and cellular debris. In some aggregates of necrotic neutrophils, there were streaming of oat-shaped cells with pale basophilc chromatin. From these results, these cases were diagnosed as purulent cranioventral bronchopneumonia. Since Mannheimia Haemolytica and Histophilus somni within the family Pasteurellaceae causing cranioventral bronchopneumonia are known to cause necrosis of neutrophils and oat-shaped cells infiltrating in the lung lesions, these cranioventral bronchopneumonia might be caused by these bacteria. Therefore, further studies will need to isolate precise causative bacterium from the lung lesions in the affected goats.