심근경색 모델 흰쥐에서 스트레스가 B-type Natriuretic Peptide 발현 및 심박변이율 변화에 미치는 영향

이삼윤(Sam Youn Lee),이미경(Mi Kyoung Lee),김남호(Nam-ho Kim),주민철(Min Cheol Joo),조항정(Hyang Jeong Jo),강지숙(Ji Sook Kang),김병숙(Byung Sook Kim),윤선식(Sun Sik Yoon),최을식(Eul Sig Choi),이문영(Moon Young Lee) 대한스트레스학회 2010 스트레스硏究 Vol.18 No.3

심박변이율의 감소는 심근 경색 환자에서 예후의 악화와 관련되어 있다. 심박변이율 중 very low frequency가 심부전환자의 예후를 판단할 수 있는 독립적 인자로서 사용이 가능하다고 보고된 바 있으며, 심실의 압력 증가에 반응해서 생성되는 B-type natriuretic peptide가 심근 경색의 예후 인자로서 사용할 수 있음 역시 보고된 바 있다. 본 연구에서는 심근경색 모델을 제작하여 일정의 회복 기간을 거친 다음 다시 일정 기간의 스트레스를 겪게 한 후 심박변이율의 변화 및 심실 조직에서의 BNP 발현 정도를 비교하여 스트레스에 의한 영향을 관찰하고자 하였다. Sprague-Dawley계 수컷 흰쥐 15마리를 대상으로 하였다. 실험동물은 인위적 스트레스 및 수술을 받지 않은 대조군(CON, n=5), 심근경색 수술을 받은 후 restraint stress를 받지 않은 군(MI+No Stress, n=5), 심근경색 수술을 받은 후 1주일 동안 restraint stress를 받은 군(MI+Stress, n=5)으로 분류하였다. 심근경색 모델 제작 직후 15분 이상 심전도의 변화를 기록하였으며, 2개월 정도 후 심근경색 수술을 받은 동물을 두 군으로 나누어 그 중 한 군에는 1주일 동안 restraint stress를 가한 후 restraint stress를 가하지 않은 동물과 심박변이율을 비교 분석하였다. 심실 조직은 좌심실 전벽 부위의 위축을 관찰할 수 있었고, 대식세포에 의하여 응고, 괴사된 심근세포의 탐식과 혈관이 풍부한 육아조직 및 섬유 변화를 볼 수 있었다. 심박변이율은 심근경색 수술을 받은 직후 심박수는 유의한 증가를 보였고, standard deviation of the normal to normal intervals (SDNN), very low frequency (VLF) 및 low frequency (LF)의 유의한 감소를 보였다. 심근경색 수술을 받았던 동물에서 두 달 정도의 회복 기간을 거친 후 스트레스를 받지 않은 군은 심박수 및 기타 심박변이율 분석에서 SDNN 값을 제외하고는 정상군과 유의한 차이를 관찰할 수 없었던 반면 1주일 동안 스트레스를 받은 군에서는 심박수가 다시 유의하게 증가했을 뿐만 아니라 SDNN, VLF 및 LF 역시 정상군과 유의한 차이를 보였다. 이상의 결과를 종합하면 심근경색 동물에 대해 1주일 동안 스트레스를 가한 결과 심박변이율이 감소하고 심실에서의 BNP 발현은 더욱 증가하여 악화함을 보여주고 있다. Diminished heart rate variability (HRV) is associated with less favorable prognosis after myocardial infarction (MI). It has been reported that very low frequency (VLF) power in HRV analysis is an independent risk predictor in patients with congestive heart failure and B-type natriuretic peptide (BNP) can be used as a prognostic factor of MI. The purpose of this study was to investigate whether stress has an effect to the changes of BNP expression and/or heart rate variability in MI model in rats. Male Sprague-Dawley rats were divided into 3 groups: control group (CON), MI group (MI+No Stress), and MI followed by stress group (MI+Stress). MI+Stress group rats were raised for a two month recovery period after the operation, followed by being exposed to restraint stress for 2 hours per day for 1 week. Electrocardiogram was recorded after the operation and the last day after 1 week of stress. The frequency components of HRV were calculated in the frequency domain such as VLF, low frequency (LF), high frequency (HF) and so on. In HRV analysis, standard deviation of the normal to normal intervals (SDNN) was significantly reduced in both groups compared to the control group. VLF and LF also were significantly reduced in MI+Stress group compared to the control groups. In addition, BNP expression in western blotting was shown the strongest bands in MI+ Stress group among experimental groups. These results suggest that BNP and HRV were aggravated by stress in MI rat model. (Korean J Str Res 2010;18:275∼285)

김채원의 〈겨울의 환〉에 나타난 문학치료적 성격 분석

류미향(Ryu, Mi-Hyang) 한국어문학회 2017 語文學 Vol.0 No.137

This article redefines the significance of motherhood expressed in Kim Ch’ae-Wǒn’s novel entitled Kyǒul ǔi hwan , which represents motherhood as a force of vitality, transcendence, and spirituality. By employing the bibliotherapic approach toward literature, I observed that the literary representation of motherhood is connected to heal human wounds. The contemporary society prevails with the mental and psychological problems, as epitomized in the concept of “fatigue society” in Han Byǒng-Ch’ǒl’s writing. Han argues for the positive side of the “fatigue society” in which people focus on deliberation and contemplation. Such pursuit of the contemplative life is suggested in Illusion of winter. Specifically, this article focuses on the expression of motherhood as an instrument of healing, and I concentrate on the two particular scenes concerning the representation of motherhood: The mother who prepares a meal and sets the table, and the mother patiently waits for her family at the door. Specifically, the mother’s preparation of food symbolizes the vitality of life, while her waiting at the door captures the transcendental personality or spirituality of the mother character. Such portrayals serve to wrap and heal the wounds and scars, since motherhood occupies the inherent and sacred realm of humanity, where pain can never reach. The vitality and spiritual nature of life and nourishment in the Illusion of winter therefore suggests its significance in modern society. Conclusively, this study illuminates the literary representation of motherhood as a site of healing.

자궁경부 상피내 병변 환자에서 LEETZ 원추절제술 후 HPV DNA Test 추적 검사의 임상적 의의

김미향,이태화,오영림,이천준,김원규 고신대학교 의과대학 2009 고신대학교 의과대학 학술지 Vol.24 No.2

Objective: This study was performed to investigate the clinical significance and timing of HPV test for follow up of patients after LEETZ treatment in CIN lesion Methods: From January 2005 to December 2008, 105 patients with CIN were treated with LLETZ at Kosin university gospel hospital, Busan, Korea. After LEETZ, HPV test and Pap smear were done after 3months. Every HPV typing was done by HPV DNA chip. Results: A total of 105 cases were enrolled in this study. There were 25 patients with CINⅠ, 38 with CINⅡ, 42 with CINⅢ. Mean age was 41.95 years (range, 23-71 years) at the time of LEETZ. Positive HPV DNA was found about 72.4% before LEETZ procedure and subtype 16 was the most common type (10.5%), follow by subtype 18 (6.6%) and subtype 58 (6.6%). During follow up after LEETZ, 65.6% had negative result after 3 month, 72.1% after 6 month, 73.9% after 9 month, 77.1% after 12 month, 79.2% after 15 month, 79.6% after 18 month, and 80.4% after 24 month. There were 2 cases of abnormal pap smear among persistent positive HPV DNA cases and no abnormal pap smear results in negative HPV DNA cases during follow up. Conclusion: Positive HPV DNA was found about 72.4% before LEETZ procedure and subtype 16 was the most common type (10.5%), follow by subtype 18 (6.6%) and subtype 58 (6.6%). We suggest that HPV DNA test is recommended per 6 Months, and close observation is recommended in persistent positive HPV DNA test cases.

키위 드레싱 제조법의 표준화 연구

김미향,이연정 동아시아식생활학회 2002 동아시아식생활학회지 Vol.12 No.5

This study was carried out to develop a kiwi dressing which was lower in calories and a more beautiful color by using Kiwi rather than mayonnaise. This study was aimed to standardize a recipe for kiwi dressing using sensory characteristics as well as to examine the changes of pH and chromaticity by the storage period. Results of this study were as follows: from the results of sensory evaluation on kiwi dressing using different kinds of oil, the pure olive oil showed the higher scores than the corn oil in the taste, fresh-sour taste and overall palatability. Sensory evaluation scores of kiwi dressings with various amounts of oil were not significantly different, which suggested that use of kiwi for a dressing was a good way to develop a low calorie dressing because the addition of 32% (160g) oil in kiwi dressing could replace the 75% oil used in mayonnaise without any significant differences in the overall palatability. From the result of sensory evaluation on kiwi dressing with different kinds of' acid, lemon juice showed the higher scores than apple vinegar in flavor. The kiwi dressing showed the highest scores in the overall palatability and fresh-sour taste when 12% (60g) onion juice was added and in as well as flavor, taste and the overall palatability at 10 days of storage. The pH values of kiwi dressing increased significantly in proportion to the storage period (p<0.001), and showed the highest pH value of 2.99 at 17 days of storage. Lightness in color showed the lowest value at 3 days of storage and the greenness of kiwi dressing increased gradually, but the yellowness decreased significantly in proportion to the storage period(p<0.001).

Collagen 성숙가교 생성에 관한 기초연구

김미향 신라대학교 자연과학연구소 1999 自然科學論文集 Vol.7 No.-

Collagen, the major constituent of connective tissue fibriles, is the most common protein in the animal world. It is widely distributed in the various tissues of vertebrates and responsible for their distinctive physiological functions. Normal tensile strength in collagen fibrils is due to intermolecular and intramolecular cross-links, which are known to alter in aging. The initial step in the formation of collagen cross-links is the deamination of specific lysyl and hydroxylysyl residues by lysyl oxidase, yielding reactive aldehydes, allysine and hydroxyallysine. Pyridinoline, a mature cross-link which is stable and non-reducible, is derived from two hydroxyallysine and one hydroxylysine residues of collagen fibrils. L-Ascorbic acid(AsA), commonly known as vitamin C, is an essential vitamin for humans, primates, and guinea pigs. AsA had important biochemical functions with various hydroxylations. In the synthesis of collagens, the enzymatic hydroxylations of proline and lysine are necessary for the production of a stable extracellular matrix and cross-links in the fibrils, respectively. To clarify the specificity of AsA in in vitro aging containing enzymatic reaction, lysyl oxidase was partially purified from the aorta of bovine. When AsA were added to the reaction mixture, pyridinoline contents were lower than the control. Moreover, it was observed that AsA is rapidly oxidized within 24 hr under this experimental condition. Supplementation with AsA may decreased pyridionline formation in the early stage of enzymatic reaction in vitro.

샐러드 드레싱에 대한 소비자의 기호도와 이용 실태 조사 연구 : 대구지역을 중심으로

김미향,이수진,김향희 동아시아식생활학회 2001 동아시아식생활학회지 Vol.11 No.5

This study was conducted to evaluate the consumers' preferences and perceptions on the salad and its dressings, via a total of 401 subjects in the Taegu area on the basis of 5-point Likert scale. Anthropometric data included the total subjects consisted of 66 females and 335 males: categorized by age, 20's (103), 30's (135). 40's (83), over 50's (80). The average size of family was 4.2 persons. According to BMI, 64.8% of the subjects belonged to the normal group. The age group that liked salad most was the 20's group, which was also the group that ate salad most frequently. The salad was perceived as food item which is convenient to eat(3.83), healthy(3.63), easy to prepare(3.63) and delicious(3.61) but rarely as a expensive item(2.44). The ingredients, used for salad preparation included fruits and vegetables (3.19) such as tomato and cucumber, Among the salad dressings, the fruit dressing was preferred the most(4.59), while the mayonnaise the least(3.59). The mayonnaise was perceived as an item with highest calories(4.1). and hardly perceived as a luxury item(2.78). The preference for the fruits dressing was highly correlated with the degree of education (p<0.05), age(p<0.001) and BMI(p<0.001). The higher the degree of education, the lower the level of age group, and the lower the BMI, the higher preference for the fruit salad dressing was demonstrated.

브래지어 착용 실태조사Ⅲ : 청년기와 중년기의 비교

나미향,권윤희,김미선 청주대학교 학술연구소 2007 淸大學術論集 Vol.9 No.-

This study is aimed at analyzing and comparing the real state of wearing brassiere between youth and mid-aged woman. The result is as follows. 1. In bust type and shape, the rate of wearing conical type in youth is estimated high at 34%,, and in mid-aged woman, that of wearing droopy type is high at 55%. Also it reveals that busts of mid-aged woman are less elastic, compared with those of youth, and bust shapes are gradually open to widen the gap between nipples. It found that mid-aged women feel more shaking their busts, when not wearing brassiere, and the larger the cup of brassiere is, the more shaking of bust they felt. Youths are more likely to wear the size 80A, 75A, and mid-age women are to wear the size 85A, 80A, 75A. And the girth under bust increases due to the obesity of back and bust parts, according as they are turning middle age. In the size of cup, the rate of wearing Cup A(73.6%) is high in youth, and in more than Cup B, the rate of mid-age women is 33.3%, higher than that of 26.4% of youth. 2. It is surveyed the reason for youths and mid-age women to wear brassiere is to prevent from the exposure of bust and its shaking, to bust up droopy breasts, or to wear a kind of habit. The rate of respondents in mid-aged women wearing brassiere to bust up droopy breasts is high, compared with that of youths. 3. Wearing brassiere confirmed the function of bust-up by 50.7%, whose rate in mid-aged women is higher than that of youth. 4. The shift of position at the bands under bust and shoulder was being felt. According to age bracket, the inconvenience of shoulder band was rated high in youths, and in the mid-aged, the shift of position at the bands under breast was high. The complaint on uneasiness was voiced because of the sense of pressure tightened by shoulder band and lateral part of armpit, and the feeling of choking at the due center of bust in which brassiere wire is inserted was complained. The rate of which was high in the mid-aged than that of youths.

맞벌이 가족의 아동복지감 척도개발

도미향 한국영유아보육학회 2003 한국영유아보육학 Vol.0 No.34

The purpose of this study was the development of a scale to assess child well-being of the Dual Income Family. The subjects were 517 children of the dual family consisted of 5th and 6th grade. %, Means, T-test, Pearson's r, Cronbach' α, KMO, factor analysis were used for data analysis. Four child well-being sense factors were extracted from the 32 items developed for this study. subscales were sense of child emotional well-being, satisfaction sense of child on the mother's role, satisfaction sense of child on the father's role and satisfaction sense of child on the mother's working. And the estimates of reliability for the scale was high. Cronbach'α was .88. The practical significance of the present study lied in the development of specific instrument for the measurement of the particular aspects of child well-being of the Dual Income Family. The development of an instrument for the measurement of such variables provides the opportunity for assessing child well-being of the Dual Income Family.

화학요법을 받는 백혈병환자의 영양 및 구강건강상태에 관한 연구

홍미순,소향숙 中央醫學社 1997 中央醫學 Vol.62 No.1

This study aimed to determine nutritional status and oral health status and obtain a basic data on the nursing intervention in reducing oral cavity complications among leukemia patients receiving chemotherapy. Subjects were 40 institutionalized patients for leukemia in Chonnal National University Hospital, Kwang-ju City. Data were collected with the aid of 2 structured instruments(Nutritional Assessment which were composed of biochemical study, B.W., Triceps Skin Fold(T.S.F.), & Mid-arm Muscle Circumference(M.A.M.C.) and Oral Health Assessment which were composed of objective oral cavity measure and subjective oral comfort measure) at two times, before and two weeks after the treatment from Dec. 1, 1995 to July, 30, 1996 Data were analyzed by using SAS-PC+, paired t-test, ANOVA, Pearson correlation coefficients. The results were summarized as follows 1. Hemoglobin, hematocrit, serum albumin, total lymphocyte count, triceps muscle skin fold, and mid-arm muscle circumference were significantly lower in post-treatment scores than pre-treatment scores(p=.0001). And BUN and creatinine level were significantly higher in post-treatment scores than pre-treatment scores(p=.0001), but B.W. was not significantly changed(p=.1674). 2. The nutritional indices after two weeks chemotherapy were significantly different depending on age, sex, marital status, B.W. Hematocrit(p=.003), serum protein(p=.053), BUN(p=.033), M.A.M.C.(p=.0015) were significantly different depending on age. Hemoglobin(p=.05), hematocrit(p=.002), BUN(p= .0001), creatinine(p=.004), T.S.F.(p=.001), and M.A.M.C.(p=.0001) were significantly higher in male than in female. M.A.M.C.(p=.0064) was significantly higher in the married than in the. unmarried. Hemoglobin(p=.02), hematocrit(p=.03), BUN(p=.02), T.S.F.(p=.0017), and M.A.M.C.(p=.0001) were significantly different depending on B.W. 3. The oral health status and comfort scores after two weeks chemotherapy were significantly lower than the scores in pre-treatment(p=.0001 ; p=.0062). 4. The oral comfort score after two weeks chemotherapy was significantly higher in male than in female(p=.0346). 5. There was a strong relationship between the oral health status and the comfort scores(r=.90). 6. There was no correlation between nutritional indices before chemotherapy and oral status score after two weeks chemotherapy. 7. There were significant relationships among nutritional indices after two weeks chemotherapy. Hemoglobin level was correlated with hematocrit(p=.001), serum protein(p=.018 9) & albumin(p=.0145) ; hematocrit was correlated with serum protein(p=.0005) & albumin (p=.0003), B.W.(p=.0101), & M.A.M.C.(p=.0003). Serum albumin was correlated with protein(p=.0001), B.W.(p=.0321), M.A.M.C.(p-.02 10) ; total lymphocyte count was correlated with T.S.F.(p=.0054) B.W. was correlated with T.S.F.(p=.004), M.A.M.C.(p=.0001), & BUN(p=.0025) ; T.S.F. was correlated with M.A.M.C.(p=.0001) ; M.A.M.C. was correlated with BUMP =.0008) ; BUN was correlated with creatinine level(p=.0003). From the above findings, it is taken that leukemia patients receiving anti-cancer chemotherapy had the experienced of nutritional defects caused by disease and treatment. Especially nutritional status after two weeks chemotherapy was significantly poor than before chemotherapy. Therefore it is necessary to implement general nutritional assessment and support nutritional management by cancer nurses.

맞벌이 가족의 아동복지감 척도에 대한 타당화 연구

도미향 한국영유아보육학회 2003 한국영유아보육학 Vol.0 No.33

The purpose of this study was to examine the validating the child well-being sense of the Dual Income Family scale to children. As the result, the child well-being sense scale contained 4variables (sense of child emotional well-being, satisfaction sense of child on the mother's role, satisfaction sense of child on the father's role, satisfaction sense of child on the mother's working) and 4 rating scale of 32items. Almost every items had high discriminating power. The reliability was satisfactory on the total readiness score. After the scale development, various validation procedures was using sample of 632. Three examples are concurrent validity, related validity, and. contrasted group validity. There appeared to be, sufficient evidence for validity of child psychological well-being instrument form the validity procedures. The present investigations contributed to understanding of child psychological well-being of the Dual Income Family. And the practical significance of the present study lied in the validation on the development of specific instrument for the measurement of the particular aspects of child well-being of the Dual Income Family. The validation of an instrument for the measurement of such variables provides the opportunity for assessing child well-being of the Dual Income Family scale.