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ZNF509S1 downregulates PUMA by inhibiting p53K382 acetylation and p53-DNA binding
Jeon, B.N.,Yoon, J.H.,Han, D.,Kim, M.K.,Kim, Y.,Choi, S.H.,Song, J.,Kim, K.S.,Kim, K.,Hur, M.W. Elsevier Science 2017 Biochimica et biophysica acta. Gene regulatory mec Vol.1860 No.9
Expression of the POK family protein ZNF509L, and -its S1 isoform, is induced by p53 upon exposure to genotoxic stress. Due to alternative splicing of the ZNF509 primary transcript, ZNF509S1 lacks the 6 zinc-fingers and C-terminus of ZNF509L, resulting in only one zinc-finger. ZNF509L and -S1 inhibit cell proliferation by activating p21/CDKN1A and RB transcription, respectively. When cells are exposed to severe DNA damage, p53 activates PUMA (p53-upregulated modulator of apoptosis) transcription. Interestingly, apoptosis due to transcriptional activation of PUMA by p53 is attenuated by ZNF509S1. Thus we investigated the molecular mechanism(s) underlying the transcriptional attenuation and anti-apoptotic effects of ZNF509S1. We show that ZNF509S1 modulation of p53 activity is important in PUMA gene transcription by modulating post-translational modification of p53 by p300. ZNF509S1 directly interacts with p53 and inhibits p300-mediated acetylation of p53 lysine K382, with deacetylation of p53 K382 leading to decreased DNA binding at the p53 response element 1 of the PUMA promoter. ZNF509S1 may play a role not only in cell cycle arrest, by activating RB expression, but also in rescuing cells from apoptotic death by repressing PUMA expression in cells exposed to severe DNA damage.
화학적 , 대사적 산화반응중 생성되는 S - oxide 를 이용한 O - ethyl S - methyl ethylphosphonothioate(1) 의 독성 기작에 관한 연구
허장현,Fukuto, T R,한대성 한국환경농학회 1991 한국환경농학회지 Vol.10 No.2
O,S-dialkyl alkylphosphonothioates 계열 유기인제 농약의 체내 작용기작을 이해하기 위하여 model 화합물 (1), O-ethyl S-methyl ethylphosphonothioate [LD_(50) (rat, oral) 4.6㎎/㎏ : K_i(bovine erythrocyte acetylcholinesterase) 303 M^(-1)min^(-1)]이 선정되었다. 이 유기인계 화합물들은 체내에서 活性化 과정을 겪으면서 毒性을 발현하는 것으로 假說되어져 왔다. 생체 내 mixed function oxidases에 의한 산화 활성화 과정을 化學的으로 재현하기 위하여 두 종류의 유기산화제 즉, meta-chloroperoxybenzoic acid와 monoperoxyphthalic acid가 사용되었고, 代謝的 산화를 재현하기 위하여 쥐 肝에서 추출한 microsomal oxidation system을 이용한 in vitro 산화반응이 시도되었다. 산화반응 중간생성물인 S-oxide의 존재가 전구물질(1)의 가설적 산화 반응경로를 통해서 간접적으로나마 충분히 확인 되어질 수 있었다. 더욱이 ethanol을 이용한 trapping실험에서 불안정한 산화중간물질인 S-oxide가 강한 phosphorylating agent라는 것이 확인되어, 전구물질 (1)로부터 산화반응을 거치면서 생성된 이 중간물질이 체내 신경전달에 중요한 역할을 하는 acetylcholinesterase를 phosphorylation하게 되고, 결국 이런 활성화 과정을 통해 이 계열의 화합물들이 독성을 발휘하는 것으로 이해될 수 있었다. O-ethyl S-methyl ethylphosphonothioate [LD_(50) (rat, oral) 4.6㎎/㎏ : K_i(bovine erythrocyte acetylcholinesterase) 303 M^(-1min-1)] was selected as a model compound to study the mode of action of O, S-dialkyl alkylphosphonothioates which have been hypothesized to be toxic via a bioactivation process. Two chemical oxidants, meta-chloroperoxybenzoic acid and monoperoxyphthalic acid, and rat liver microsomal oxidases were used to mimic the action of mixed function oxidases on the model compound. The formation of S-oxide, a very unstable active intermediate, was proposed based on the identification of metabolic products. Furthermore, a trapping experiment with ethanol showed that the unstable intermediate S-oxide had the ability to phosphorylate acetylcholinesterase which is an important enzyme in nerve systems. The S-oxide intermediates are presumed to be responsible for the toxicity of O,S-dialkyl alkylphosphonothioates.
전주 근교 농장의 자돈에서 면역형광항체법을 이용한 돼지콜레라 혈청 중화항체가 조사
허창열,김영진,노수일,김진구,송희종,김종면 全北大學校 附設 畜産開發硏究所 1991 畜産開發硏究報告 Vol.4 No.-
In order to evaluate the effect of vaccinantion for hog-cholera virus, sera were collected from each 20 piglet out of 26-32 days old before vaccination, and then from the 14th and 28th day post-vaccination(PV) with LOM live virus vaccine at the J and C swine ranches in the suburbs of Chonju. Monoclonal antibody(MAb) against ALD strain of hog cholera virus(HCV) and goal antimouse FITC-conjugates were employed in indirect immunofluorescence(IF) test for the titration of HCV and neuralization IF test for serum neutralization(SN)-Ab-titers against HCV. SN-Ab-titers(M±S.D.) were <2±0 in all piglets before vaccination at the both swine ranches. Whereas SN-Ab-titers of the J and C swine ranches were 111.2±115.08 and 144±121.7 at the 14th day PV and were 361.6±279.6 and 723.2±966.2 at the 28th day PV, respectively. Compared with Duncan's test, SN-Ab-titers were not significantl difference between the H and C swine ranches of the 14th day PV, but were significantly difference at the 28th day PV (P<0.0009). Therefore, SN-Ab-titers were significantly difference between the 14th and 28th day at the J or C ranches, respectively(P<0.0007, P<0.0114). Excepting SN-Ab-titer was1:16 in one piglet of the 14th day PV at the J swine ranch, defensible levels of SN-Ab-titers could be detected in all piglets PV of the both swine ranches. It is suggested that varied dffect of vaccination against HCV may be depended by feed and mangement, enviroment, sanitary conditions, breed and pedigree or vaccination program, etc.
알미늄 다결정에서 결정립의 결정방위가 피로변형에 미치는 영향
허성강,남수우,맹선재,천성순 대한금속재료학회(대한금속학회) 1977 대한금속·재료학회지 Vol.15 No.5
알미늄多結晶(純度 99.9%)을 두 應力 사이에서 引張疲勞시켜 多結晶內의 各 結晶粒에 形成되는 slip line을 觀察하여 이들과 各 結晶粒의 方位 즉 Schmid factor와의 關係를 調査하였다. 알미늄 多結晶의 結晶粒은 直徑 約 1.5∼3.0㎜의 크기로 성장시켰고 Schmid factor는 Laue backre flection 촬영으로 決定한 各 結晶粒의 方位에서 計算하였다. 試片은 引張應力 189∼47g/㎟ 또는 198∼32g/㎟ 사이에서 70cpm의 낮은 周期로 反復疾勞시켰으며 10², 10³및 10⁴cyc1e 후에 slip line의 생성 및 分布상태를 光學顯微鏡으로 觀察하였다. Schmid factor 큰 結晶粒일 수록 slip band의 形成에 必要한 疲勞 cycle의 수가 적었으며 Schmid factor가 작은 結晶粒(Schmid factor: 0.34 및 0.37)에서는 10³또는 10⁴cycle의 試驗 후에도 slip band가 觀察되지 않았다. The relation between formation and distribution of slip bands in the grains of aluminum polycrystals (purity: 99.9%) and crystal orientation of each grain have been studied after tensile fatigue stressing of the samples. Grains of the samples were grown to the size of 1.5∼3.0㎜ diameter by heat treatment. Crystal orientation and Schmid factor of each grain were determined by means of Laue back reflection. Cyclic tensile stresses were applied to the samples between 189 and 47g/㎟ or 198 and 32g/㎟ at a low frequency of 70 cpm, and the formation and distribution of slip bands were observed by optical microscope after the cyclic stressing of 10², 10³, and 10⁴ cycles. As the value of Schmid factor of a grain was increased, the number of fatigue cycles, in which slip bands are formed, was decreased, Not any slip bands were found in the grains of low Schmid factor values, such as 0.34 and 0.37, even after 10⁴ cycles.
포장방법과 저장조건이 돈육 등심의 저장성 및 보수력에 미치는 영향
허선진,주선태,오성현,김영직,김영환,이정일,박구부 한국동물자원과학회 2001 한국축산학회지 Vol.43 No.1
To investigate the effects of packaging method and storage condition on meat quality, a total of eight pork loins were selected from a commercial meat plant at 24 hr postmortem. The loins were cut to 2.5㎝ thick steaks and were randomly assigned to the five treatment combination; vacuum packaging and submersion in chilled salt water(SCSW)(T1), non-vacuum packaging and submersion in chilled salt water (T2), vacuum packaging and storage in conventional refrigerator(SCR)(T3), non-vacuum packaging and storage in conventional refrigerator(T4), and wrap packaging and storage in conventional refrigerator(TS). All samples were stored at 0℃ for 20 days to measure color of meat(CIE L*, a*. b*), pH, drip loss, purge loss %, lipid oxidation (TBARS; thiobarbituric acid reactive substances) and total plate counts (TPC). CIE L* and b* value of SCSW(T1, 2) samples were significantly(P$lt;0.05) lower than SCR samples when they were measured at storage 20 days. The sample of SCSW(T1, 2) showed significantly(P$lt;0.05) lower pH than those of SCR(T3, 4, 5) during storage. The sample of SCSW(T1, 2) showed significantly(P$lt;0.05) higher Drip loss than those of SCR(T3, 4, 5) during 5 days storage. T2 showed the lowest purge loss % whereas T3 remarked the highest purge loss %. The samples of SCSW showed significantly(P$lt;0.05) lower TBARS than those of SCR during storage. TPC was samples of SCSW(T1, 2) showed significantly(P$lt;0.05) lower than other treatments. These results suggested that NVP(non-vacuum packaging) and SCSW could reduce purge loss and discoloration, and extend shelf-life of pork loin.