Suppression of long non-coding RNA PCAT19 inhibits glioma cell proliferation and invasion, and increases cell apoptosis through regulation of MELK targeted by miR-142-5p

Yu‑hua Xie,Jiao Hu 한국유전학회 2020 Genes & Genomics Vol.42 No.11

Background Glioma has the chief type of primary brain tumors worldwide. The glioma may be controlled by regulatorsincluding some lncRNAs, miRNAs, and proteins. Objective Our study aims to discover the underlying mechanism for lncPCAT19/miR-142-5p/MELK axis in gliomaprogression. Methods The clinical samples were from patients with gliomas in our Hospital. Hematoxylin–eosin staining (H&E) wasapplied to determine the clinical pathological changes. Real time PCR was performed to measure the levels of lncPCAT19,miR-142-5p, MELK, and expression of other genes. Western blot was conducted to detect the protein level of MELK. RIPassay was performed to analyze the interaction between lncPCAT19 and miR-142-5p, and dual-luciferase reporter assay wasused to determine the binding site between lncPCAT19 and miR-142-5p. CCK-8, colony formation assay, flow cytometry,and trans-well assay were carried out to confirm cell proliferation, colony formation, apoptosis, and invasion, respectively. Results LncPCAT19 was increased in cancer tissues. Then, lncPCAT19 could interact with and down-regulate miR-142-5p. Knockdown of lncPCAT19 distinctly inhibited tumor growth in vivo. Interfering lncPCAT19/overexpression of miR-142-5pdecreased glioma cell proliferation, colony formation and invasion, and promoted cell apoptosis by down-regulating expressionof Cyclin B1, CDK2, N-cadherin, Bcl-2, and by up-regulating expression of Bax and E-cadherin. Moreover, overexpressionof lncPCAT19 overturned tumor-suppressing role of miR-142-5p in cells. Additionally, lncPCAT19 and miR-142-5psynergistically regulated expression of MELK. In conclusion, lncPCAT19 enhanced glioma development via increasingMELK by performing as a sponge of miR-142-5p. Conclusions LncPCAT19 promotes glioma progression by sponging miR-142-5p to upregulate MELK levels. Thus, lncPCAT19/miR-142-5p/MELK signaling would be a potential target for glioma treatment.

중증 만성정신질환자 수의 추산 : Through Two Community Surveys 두 지역사회 조사를 중심으로

홍진표,김윤,배재남,정유진,강영호,양병국,김용익,김병후,이철,조맹제 大韓神經精神醫學會 1998 신경정신의학 Vol.37 No.2

국내 중증 만성정신질환자 수를 추정하기 위하여 일 농촌 및 대도시 지역의 지역의료보험 및 의료보호 대상자 중 1993년 1년간 의료기관에서 정신증 상병(ICD-9 code 상 290-299) 및 알콜 남용/중독, 성격장애 및 정신박약 진단하에 치료를 받은 적이 있는 사람에 대해 설문조사를 실시하였다. 연구자들이 개발한 진단도구를 이용하여 진단을 평가하고, Strauss-Carpenter scale 등을 이용하여 기능 장애를 평가하고 유병기간 등을 조사하였다. 결과를 요약하면 다음과 같다. 1) 진단평가상 정신분열병군(61.1%)이 가장 많았으며, 정동장애군(23.7%), 알콜장애군(11.2%), 기질성 정신장애군(4.0%)의 순서이었다. 2) 직업활동정도는 진단군별로 차이가 많았다. 지난 1년간 직업활동이 6개월 미만이거나 전혀 없는 환자 비율이 정신분열병군은 67.2%, 알콜장애군 52.0%, 정동장애20.8%의 순으로 정신분열병군이 가장 불량하였다. 3) 사회활동도 진단군별로 차이가 많아서 지난 1년간 의도적, 사회활동이 없는 비율이 정신분열병군이 가장 높았고(72.3%), 알콜장애군(32.0%), 정동장애군(20.7%)의 순이었다. 4) 국내 의료보험 및 사회복지시설 이용 자료 등을 토대로 본 조사 결과를 적용한 결과 국내중증 만성 정신질환자의 수는 약 8만 7천명으로 추산되었다. Objectives: The purpose of this study was to estimate the number of serious and persistent mentally ill patients in Korea. Methods : Study populations were recruited from medical insurance data of two district areas ; one rural and the other urban areas. Subject were all mentally ill who had ever visited any hospital in 1993 and were diagnosed as having psychosis, personality disorder and drug abuse. We conducted a home visiting survey applying semi-structured diagnostic interview, assessing the level of social adjustment with Strauss-Carpenter Scale, and past psychiatric history by the trained researchers, in October and November,1994. Results : According to diagnostic assessment schizophrenic patients constituted the major portion(61.1%) of study subjects. The distribution of occupational functions and social contacts was variable according to diagnosis. Most schizophrenic patients were unemployed or worked less than 6 months in the previous year(67.2%), and showed poor social contact(72.3%). But affective disorder patients were relatively independent in job maintenance and social relationship. Alcoholic patients lay between those of schizophrenic and affective disorder groups. It was estimated that about 87,000 adult Koreans were suffering from chronic mental disorders which seriously interfere with their daily life. Conclusions : Serious and persistent mentally ill patients were common in Korea. Community support program should be provided for them.

[PB-0100] Functional Analysis of Heat-induced RING-type E3 Ligases in Sorghum: Expression Patterns, Subcellular Localizations and Interaction Partners

Yu Kyong Hu(Yu Kyong Hu),Hong-Ju Lee(Hong-Ju Lee),Jeong Ho Shin(Jeong Ho Shin),Sang Hun Kim(Sang Hun Kim),Min Seok Choi(Min Seok Choi),Hyeon Ung Seo(Hyeon Ung Seo),Cheol Seong Jang(Cheol Seong Jang),S 한국육종학회 2022 한국육종학회 공동학술발표집 Vol.2022 No.-

Enhancement of Scenedesmus sp. LX1 Biomass Production and Lipid Accumulation Using Iron in Artificial Wastewater and Domestic Secondary Effluent

( Wen Yu Zhao ),( Jun Yi Yu ),( Yin Hu Wu ),( Yu Hong ),( Hong Ying Hu ) 한국미생물생명공학회(구 한국산업미생물학회) 2014 한국미생물·생명공학회지 Vol.42 No.2

While coupling wastewater treatment with microalgal bioenergy production is very promising, new approaches are needed to enhance microalgal growth and lipid accumulation in wastewater. Therefore, this study investigated the effect of iron on the growth, nutrient removal, and lipid accumulation of Scenedesmus sp. LX1 in both artificial wastewater and domestic secondary effluents. When increasing the iron concentration from 0 to 2 mg/l in the artificial wastewater, the biomass production of Scenedesmus sp. LX1 increased from 0.17 to 0.54 g/l; the nitrogen and phosphorus removal efficiency increased from 15.7% and 80.6% to 97.0% and 99.2%, respectively; and the lipid content was enhanced 84.2%. The relationship between the carrying capacity/maximal population growth rate of Scenedesmus sp. LX1 and the initial iron concentration were also in accordance with the Monod model. Furthermore, when increasing the iron concentration to 2 mg/l in four different domestic secondary effluent samples, the lipid content and lipid production of Scenedesmus sp. LX1 was improved by 17.4-33.7% and 21.5-41.8%, respectively.

Enhancement of Scenedesmus sp. LX1 Biomass Production and Lipid Accumulation Using Iron in Artificial Wastewater and Domestic Secondary Effluent

Zhao, Wen-Yu,Yu, Jun-Yi,Wu, Yin-Hu,Hong, Yu,Hu, Hong-Ying The Korean Society for Microbiology and Biotechnol 2014 한국미생물·생명공학회지 Vol.42 No.2

While coupling wastewater treatment with microalgal bioenergy production is very promising, new approaches are needed to enhance microalgal growth and lipid accumulation in wastewater. Therefore, this study investigated the effect of iron on the growth, nutrient removal, and lipid accumulation of Scenedesmus sp. LX1 in both artificial wastewater and domestic secondary effluents. When increasing the iron concentration from 0 to 2 mg/l in the artificial wastewater, the biomass production of Scenedesmus sp. LX1 increased from 0.17 to 0.54 g/l; the nitrogen and phosphorus removal efficiency increased from 15.7% and 80.6% to 97.0% and 99.2%, respectively; and the lipid content was enhanced 84.2%. The relationship between the carrying capacity/maximal population growth rate of Scenedesmus sp. LX1 and the initial iron concentration were also in accordance with the Monod model. Furthermore, when increasing the iron concentration to 2 mg/l in four different domestic secondary effluent samples, the lipid content and lipid production of Scenedesmus sp. LX1 was improved by 17.4-33.7% and 21.5-41.8%, respectively.

A Novel Active Image AuthenticationSchemefor Block Truncation Coding

Chang-Ming Wu,Yu-Chen Hu,Kuo-Yu Liu,Jun-Chou Chuang 보안공학연구지원센터 2014 International Journal of Signal Processing, Image Vol.7 No.5

In this paper, a simple active image authentication scheme for the compressed images of block truncation coding (BTC) is proposed. In this scheme, the authentication codes of the compressed blocks are generated from the random value induced by the random seed. The authentication code of each compressed block is embedded into the bit map. The bit length of each authentication code can be chosen according to the user’s requirement. The experimental results indicate that the proposed scheme detects the tampered areas clearly and keeps good image qualities of the embedded images. Meanwhile, a low computational cost is required in the proposed scheme.

The Blood Oxygenation T2* Values of Resectable Esophageal Squamous Cell Carcinomas as Measured by 3T Magnetic Resonance Imaging: Association with Tumor Stage

Yu-lian Tang,Xiao-ming Zhang,Zhi-gang Yang,Yu-cheng Huang,Tian-wu Chen,Yan-li Chen,Fan Chen,Nan-lin Zeng,Rui Li,Jiani Hu 대한영상의학회 2017 Korean Journal of Radiology Vol.18 No.4

Objective: To explore the association between the blood oxygenation T2* values of resectable esophageal squamous cell carcinomas (ESCCs) and tumor stages. Materials and Methods: This study included 48 ESCC patients and 20 healthy participants who had undergone esophageal T2*-weighted imaging to obtain T2* values of the tumors and normal esophagi. ESCC patients underwent surgical resections less than one week after imaging. Statistical analyses were performed to identify the association between T2* values of ESCCs and tumor stages. Results: One-way ANOVA and Student-Newman-Keuls tests revealed that the T2* value could differentiate stage T1 ESCCs (17.7 ± 3.3 ms) from stage T2 and T3 tumors (24.6 ± 2.7 ms and 27.8 ± 5.6 ms, respectively; all ps < 0.001). Receiver operating curve (ROC) analysis showed the suitable cutoff T2* value of 21.3 ms for either differentiation. The former statistical tests demonstrated that the T2* value could not differentiate between stages T2 and T3 (24.6 ± 2.7 ms vs. 27.8 ± 5.6 ms, respectively, p > 0.05) or between N stages (N1 vs. N2 vs. N3: 24.7 ± 6.9 ms vs. 25.4 ± 4.5 ms vs. 26.8 ± 3.9 ms, respectively; all ps > 0.05). The former tests illustrated that the T2* value could differentiate anatomic stages I and II (18.8 ± 4.8 ms and 26.9 ± 5.9 ms, respectively) or stages I and III (27.3 ± 3.6 ms). ROC analysis depicted the same cutoff T2* value of 21.3 ms for either differentiation. In addition, the Student’s t test revealed that the T2* value could determine grouped T stages (T0 vs. T1–3: 17.0 ± 2.9 ms vs. 25.2 ± 6.2 ms; T0–1 vs. T2–3: 17.3 ± 3.0 ms vs. 27.1 ± 5.3 ms; and T0–2 vs. T3: 18.8 ± 4.2 ms vs. 27.8 ± 5.6 ms, all ps < 0.001). ROC analysis indicated that the T2* value could detect ESCCs (cutoff, 20 ms), and discriminate between stages T0–1 and T2–3 (cutoff, 21.3 ms) and between T0–2 and T3 (cutoff, 20.4 ms). Conclusion: The T2* value can be an additional quantitative indicator for detecting ESCC except for stage T1 cancer, and can preoperatively discriminate between some T stages and between anatomic stages of this tumor.

Granular Effects on Depositional Processes of Debris Avalanches

Yu-xiang Hu,Hai-bo Li,Shun-chao Qi,Gang Fan,Jia-wen Zhou 대한토목학회 2020 KSCE JOURNAL OF CIVIL ENGINEERING Vol.24 No.4

Debris avalanches generally have massive mobility and extensive deposited area, which have strong relation to the granular effect. The granular effect is an outcome from the interaction (based on frictional mechanism) among particles. Discrepancy of grain composition, for instance the difference of particle size and their proportion of particles, is a key factor to influence granular effect. A series of laboratory tests have been conducted to research the granular effects on depositional processes of debris avalanches containing fine- and coarse-grained particles. The velocity of debris front, the morphology characteristics and runout distance of the debris avalanche are considered in detail, and the mass parameter and the size parameter are introduced in analysis for the research of granular effect. The experimental results indicate that the granular effects can decrease the dissipation of momentum and increase the accumulation region, leading to further runout distance and more extensive deposited areas. Further, the velocity, the range of depositions of debris avalanches are larger than that in other conditions when the proportion of fine-grained particles is 30%, especially the materials consist of heterogeneous granular. It means there have a critical mass parameter in experiments which lead to the high efficiency of granular effects, and the granular effect is weakening in homogeneous granular composition.

A comparison of structural performance enhancement of horizontally and vertically stiffened tubular steel wind turbine towers

Yu Hu,Jian Yang,Charalambos C. Baniotopoulos,Feiliang Wang 국제구조공학회 2020 Structural Engineering and Mechanics, An Int'l Jou Vol.73 No.5

Stiffeners can be utilised to enhance the strength of thin-walled wind turbine towers in engineering practise, thus, structural performance of wind turbine towers by means of different stiffening schemes should be compared to explore the optimal structural enhancement method. In this paper two alternative stiffening methods, employing horizontal or vertical stiffeners, for steel tubular wind turbine towers have been studied. In particular, two groups of three wind turbine towers of 50m, 150m and 250m in height, stiffened by horizontal rings and vertical strips respectively, were analysed by using FEM software of ABAQUS. For each height level tower, the mass of the stiffening rings is equal to that of vertical stiffeners each other. The maximum von Mises stresses and horizontal sways of these towers with vertical stiffeners is compared with the corresponding ring-stiffened towers. A linear buckling analysis is conducted to study the buckling modes and critical buckling loads of the three height levels of tower. The buckling modes and eigenvalues of the 50m, 150m and 250m vertically stiffened towers were also compared with those of the horizontally stiffened towers. The numbers and central angles of the vertical stiffeners are considered as design variables to study the effect of vertical stiffeners on the structural performance of wind turbine towers. Following an extensive parametric study, these strengthening techniques were compared with each other and it is obtained that the use of vertical stiffeners is a more efficient approach to enhance the stability and strength of intermediate and high towers than the use of horizontal rings.

Intelligent operation of the hedgerow machine Design

Hu Yu 한국콘텐츠학회 2020 한국콘텐츠학회 ICCC 논문집 Vol.2020 No.11