Use of In Vivo-Induced Antigen Technology to Identify In Vivo-Expressed Genes of Campylobacter jejuni During Human Infection

( Yuan Qing Hu ),( Jin Lin Huang ),( Qiu Chun Li ),( Yu Wei Shang ),( Fang Zhe Ren ),( Yang Jiao ),( Zhi Cheng Liu ),( Zhi Ming Pan ),( Xin An Jiao ) 한국미생물 · 생명공학회 2014 Journal of microbiology and biotechnology Vol.24 No.3

Campylobacter jejuni is a prevalent foodborne pathogen worldwide. Human infection by C. jejuni primarily arises from contaminated poultry meats. Genes expressed in vivo may play an important role in the pathogenicity of C. jejuni. We applied an immunoscreening method, in vivo-induced antigen technology (IVIAT), to identify in vivo-induced genes during human infection by C. jejuni. An inducible expression library of genomic proteins was constructed from sequenced C. jejuni NCTC 11168 and was then screened using adsorbed, pooled human sera obtained from clinical patients. We successfully identified 24 unique genes expressed in vivo. These genes were implicated in metabolism, molecular biosynthesis, genetic information processing, transport, and other processes. We selected six genes with different functions to compare their expression levels in vivo and in vitro using real-time RT-PCR. The results showed that the selected six genes were significantly upregulated in vivo but not in vitro. In short, these identified in vivo-induced genes may contribute to human infection of C. jejuni, some of which may be meaningful vaccine candidate antigens or diagnosis serologic markers for campylobacteriosis. IVIAT may present a significant and efficient method for understanding the pathogenicity mechanism of Campylobacter and for finding targets for its prevention and control.

Effect of Corticosterone Administration on Small Intestinal Weight and Expression of Small Intestinal Nutrient Transporter mRNA of Broiler Chickens

Hu, X.F.,Guo, Yuming,Huang, B.Y.,Zhang, L.B.,Bun, S.,Liu, D.,Long, F.Y.,Li, J.H.,Yang, X.,Jiao, P. Asian Australasian Association of Animal Productio 2010 Animal Bioscience Vol.23 No.2

The effects of corticosterone (CORT) administration on the weight of small intestine and the expression of nutrient transporter mRNA in the small intestine of broiler chickens (Gallus gallus domesticus) were investigated. One hundred and eight sevenday-old birds were randomly divided into two equal groups comprising a control group (CTRL) and an experimental group (CORT). CTRL birds were fed a basal diet and the CORT birds were fed a basal diet containing 30 mg corticosterone/kg from d 8 to 21. At 21 d of age, average daily feed intake (ADFI), serum corticosterone level, small intestinal absolute wet weight and relative weight, and relative abundance of SGLT1, CaBP-D28k, PepT1 mRNA in the duodenum and L-FABP mRNA in the jejunum were determined. The results showed that serum corticosterone level, liver weight and small intestinal relative weight (small intestinal wet weight/body weight) of CORT chickens were about 30.15%, 26.72% and 42.20% higher, respectively, than in the CTRL group (p<0.05). CORT birds had relative mRNA abundance of CaBP-D28k and PepT1 in the duodenum, and L-FABP in the jejunum which was 1.77, 1.37 and 1.94 fold higher, respectively, than in the CTRL group (p<0.05); the relative abundance of SGLT1 was 1.67 fold higher than in the CTRL group (p = 0.097). ADFI, small intestinal wet weight and length in CORT-treated broiler chickens was about 29.11%, 31.12% and 12.35% lower, respectively, than in the CTRL group (p<0.05). In conclusion, corticosterone administration lowered the wet weight but increased the relative weight of the small intestine and the expression of intestinal nutrient transporter mRNA of broiler chickens.

THE PERIODIC JACOBI MATRIX PROCRUSTES PROBLEM

Jiao-fen Li,XI-YAN HU 한국전산응용수학회 2010 Journal of applied mathematics & informatics Vol.28 No.3

The following ”Periodic Jacobi Procrustes” problem is stud-ied: find the Periodic Jacobi matrix X which minimizes the Frobenius (or Euclidean) norm of AX −B, with A and B as given rectangular matrices. The class of Procrustes problems has many application in the biological,physical and social sciences just as in the investigation of elastic struc-tures. The different problems are obtained varying the structure of the matrices belonging to the feasible set. Higham has solved the orthogonal,the symmetric and the positive definite cases. Andersson and Elfving have studied the symmetric positive semidefinite case and the (symmetric) el-ementwise nonnegative case. In this contribution, we extend and develop these research, however, in a relatively simple way. Numerical difficulties are discussed and illustrated by examples.

Synthesis of Needle-like Aragonite from Limestone without Calcinations in the Presence of Magnesium Sulfate

Hu, Zeshan,Shao, Minghao,Cai, Qiang,Jiao, Zhaojie,Zhong, Chenhua,Deng, Yulin The Korean Society for Composite Materials 2009 Advanced composite materials Vol.18 No.2

Much attention has been paid to the processing of inorganic whisker, especially calcium carbonate whisker, which can be used as reinforcement materials of polymer composite due to its low price. Unfortunately, the present synthesis technique of calcium carbonate whisker starts from calcinations of limestone, which involves high energy consumption and furthermore is a highly environment polluting reaction. In this report, needle-like aragonite was synthesized with a reversible solution reaction from limestone without calcination. Optical microscopy, scanning electron microscopy (SEM) and X-ray diffraction (XRD) techniques were used to characterize the morphology and crystal structure of intermediates as well as that of the product, aragonite. GCC (grinding calcium carbonate) powder was dissolved in an aqueous solution of magnesium sulfate with reflux and air flush. EDTA titration was used to evaluate reaction rate of the dissolution. A kinetics equation of the dissolution reaction was constructed, which displayed second-order kinetics with respect to the concentration of magnesium sulfate. A rate constant of $0.0015\;l^{-3}{\cdot}mol^{-1}{\cdot}h^{-1}$ was obtained. The dissolution reaction gave fiber-like magnesium hydroxide sulfate and gypsum crystal. Then needle-like aragonite with a length of $9.13\;{\pm}\;1.02\;{\mu}m$ and an aspect ratio of $5.64\;{\pm}\;1.37$ was synthesized from the dissolution product with $CO_2$ bubbling at $70^{\circ}C$.

Tumor Targeting and pH-Responsive Polyelectrolyte Complex Nanoparticles Based on Hyaluronic Acid-Paclitaxel Conjugates and Chitosan for Oral Delivery of Paclitaxel

Jiao Li,Pingsheng Huang,Longlong Chang,Xingwen Long,Anjie Dong,Jinjian Liu,Liping Chu,Fuqiang Hu,Jianfeng Liu,Liandong Deng 한국고분자학회 2013 Macromolecular Research Vol.21 No.12

A new platform of paclitaxel (PTX) for application as an oral delivery system was developed, by combining the pH sensitivity of polyelectrolyte complex nanoparticles (CNPs) and the active targeting of hyaluronic acid (HA). Chitosan/hyaluronic acid-paclitaxel (CS/HA-PTX) CNPs were prepared by coating the CS onto the HA-PTX nanoparticles (NPs), and characterized by Fourier-transform infrared spectroscopy (FTIR), nuclear magnetic resonance (1H NMR), transmission electron microscopy (TEM) and high-performance liquid chromatography (HPLC). HA-PTX conjugates could self-assemble into NPs in aqueous solution with an average size of 100±5 nm, and the PTX content of HA-PTX conjugates was 10.6 wt%. The CS/HA-PTX CNPs had a smaller size and higher PTX content when the ratio of positive charge to negative charge was 2:1. The in vitro release of PTX from CNPs was pH-responsive,suggesting that the CS shell could prevent the breakage of the ester bond in HA-PTX NPs in acidic pH conditions. HA-PTX NPs exhibited higher cellular uptake than free PTX against HepG2 cells via receptor-mediated endocytosis. PTX could accumulate remarkably into tumor sites after oral administration of CNPs. These results indicate that the CNP drug delivery system has great potential for applications in the oral administration of hydrophobic drugs.

A Novel Multiple Attribute Decision Making Framework based on Hamacher Operations in Patron Driven Acquisitions Mode of Library Management

Jiao Li,Dong Hu,Chao Tang,Jufang Xie 보안공학연구지원센터 2016 International Journal of u- and e- Service, Scienc Vol.9 No.5

As a fuzzy set extension, the hesitant set is effectively used to model situations where it is allowable to determine several possible membership degrees of an element to a set due to the ambiguity between different values. Some new hesitant fuzzy operational rules are introduced based on the Hamacher t-conorm and t-norm, whereby we present a multi attribute decision making method under hesitant fuzzy information. In the daily management of library, book selection problem usually is very complex and unstructured, because variety of uncontrollable and unpredictable factors affects the evaluation and decision-making process at different levels. By integrating the multiple attribute decision making method with (Patron Driven Acquisitions, PDA) mode, a novel multiple attribute decision making framework based on Hamacher operations in PDA mode is proposed to select the best book providing the highest satisfaction for the attributes determined in this paper. Finally, a case study is so demonstrated to verify the reliability and applicability of the proposed framework.

Clinical Features of Multiple Primary Malignancies: a Retrospective Analysis of 72 Chinese Patients

Jiao, Feng,Yao, Li-Juan,Zhou, Jin,Hu, Hai,Wang, Li-Wei Asian Pacific Journal of Cancer Prevention 2014 Asian Pacific journal of cancer prevention Vol.15 No.1

There is a scarcity of reports addressing patients with multiple primary malignancies (MPM), especially for Chinese cses. The aim of this study was to present a detailed analysis of Chinese patients presenting with at least two primary malignancies. Particularly, the clinical characteristics and survival between synchronous and metachronous MPM were compared. Out of 6,545 cases, 72 patients (1.1%) including 39 males (54.2%) and 33 females (45.8%) were diagnosed as MPM, giving a male: female sex ratio of 1.2:1. Of these, there were 16 (22.2%) cases of synchronous MPM (7 males, 9 females), 55(76.4%) metachronous (31 males, 24 females), and 1 "mixed form". For first tumor diagnosis time, synchronous MPM patients generally presented later than the metachronous cases. The top three sites for malignancies with metachronous MPM were colorectum, head and neck, and lung, while for synchronous they were lung, colorectum and breast. Among MPM patients, the median survival time was 15.7 years and the 5-year survival was 56%, and there was statistical difference in MPM categories (P < 0.05). The median survival time was 17.3 years and 3.8 years for metachronous and synchronous MPM patients, respectively. In comparison with synchronous MPM patients, those metachronous had a longer survival. This studies increase understanding of the clinical features of Chinese MPM patients and suggest that those presenting with metachronous cancers have a higher incidence and a better prognosis.

Isolation, Molecular Characterization and Antibiotic Susceptibility Pattern of Vibrio parahaemolyticus from Aquatic Products in the Southern Fujian Coast, China

Yuanqing Hu,Fengxia Li,Yixian Zheng,Xinan Jiao,Liqing Guo 한국미생물·생명공학회 2020 Journal of microbiology and biotechnology Vol.30 No.6

Vibrio parahaemolyticus is a major gastroenteritis-causing pathogen in many Asian countries. Antimicrobial resistance in V. parahaemolyticus has been recognized as a critical threat to food safety. In this study, we determined the prevalence and incidence of antimicrobial resistance in V. parahaemolyticus in the southern Fujian coast, China. A total of 62 isolates were confirmed in retail aquatic products from June to October of 2018. The serotype O3:K6 strains, the virulence genes tdh and trh, antibiotic susceptibility and molecular typing were investigated. Then plasmid profiling analysis and curing experiment were performed for multidrug-resistant strains. The results showed that the total occurrence of V. parahaemolyticus was 31% out of 200 samples. Five strains (8.1%) out of 62 isolates were identified as the V. parahaemolyticus O3:K6 pandemic clone. A large majority of isolates exhibited higher resistance to penicillin (77.4%), oxacillin (71%), ampicillin (66.1%) and vancomycin (59.7%). Seventy-one percent (44/62) of the isolates exhibited multiple antimicrobial resistance. All 62 isolates were grouped into 7 clusters by randomly amplified polymorphic DNA, and most of the isolates (80.6%) were distributed within cluster A. Plasmids were detected in approximately 75% of the isolates, and seven different profiles were observed. Seventy-six percent (25/33) of the isolates carrying the plasmids were eliminated by 0.006% SDS incubated at 42°C, a sublethal condition. The occurrence of multidrug-resistant strains could be an indication of the excessive use of antibiotics in aquaculture farming. The rational use of antimicrobial agents and the surveillance of antibiotic administration may reduce the acquisition of resistance by microorganisms in aquatic ecosystems.

Optimization for the direction of arrival estimation based on single acoustic pressure gradient vector sensor

Xu-hu Wang,Jian-feng Chen,Jing Han,Ya-meng Jiao 대한조선학회 2014 International Journal of Naval Architecture and Oc Vol.6 No.1

The optimization techniques are explored in the direction of arrival (DOA) estimation based on single acoustic pressure gradient vector sensor (APGVS). By analyzing the working principle and measurement errors of the APGVS, acoustic intensity approaches (AI) and the minimum variance distortionless response beamforming approach based on single APGVS (VMVDR) are deduced. The radius to wavelength ratio of the APGVS must be not bigger than 0.1 in the actual application, otherwise its DOA estimation performance will degrade significantly. To improve the ro-bustness and estimation performance of the DOA estimation approaches based on single APGVS, two modified proce-ssing approaches based on single APGVS are presented. Simulation and lake trial results indicate that the performance of the modified approaches based on single APGVS are better than AI and VMVDR approaches based on single APGVS when the radius to wavelength ratio is not bigger than 0.1, and the two modified DOA estimation methods have excel¬lent estimation performance when the radius to wavelength ratio is bigger than 0.1.

THE PERIODIC JACOBI MATRIX PROCRUSTES PROBLEM

Li, Jiao-Fen,Hu, Xi-Yan The Korean Society for Computational and Applied M 2010 Journal of applied mathematics & informatics Vol.28 No.3

The following "Periodic Jacobi Procrustes" problem is studied: find the Periodic Jacobi matrix X which minimizes the Frobenius (or Euclidean) norm of AX - B, with A and B as given rectangular matrices. The class of Procrustes problems has many application in the biological, physical and social sciences just as in the investigation of elastic structures. The different problems are obtained varying the structure of the matrices belonging to the feasible set. Higham has solved the orthogonal, the symmetric and the positive definite cases. Andersson and Elfving have studied the symmetric positive semidefinite case and the (symmetric) elementwise nonnegative case. In this contribution, we extend and develop these research, however, in a relatively simple way. Numerical difficulties are discussed and illustrated by examples.