Mix LPS-ELISA법을 이용한 살모넬라균 복합감염항체 동시 모니터링

이희수,임숙경,우승룡,이유영,김종염,주이석,김종만,Lee, Hee-soo,Im, Suk-kyoung,Woo, Seng-ryong,Yi, You-young,Kim, Jong-yeom,Joo, Yi-seok,Kim, Jong-man 대한수의학회 1998 大韓獸醫學會誌 Vol.38 No.4

Salmonellosis caused by a number of serotypes of Salmonella is an infectious, acute or chronic, zoonotic disease and characterized by enteritis and diarrhea, septicemia in animal. In these studies we investigated the prevalent serotypes of Salmonella causing animal salmonellosis in Korea and the 71 strains of Salmonella spp. were isolated from materials such as mesenteric lymph nodes, fecal samples from slaughtered animal. With the identification test results, the most prevalent serotypes were, in order, S stanley 31 strains (43.7%), S typhimurium 19 strains (26.8%) and S montevideo 11 strains (15.5%), respectively. And we could establish the method for detection of antibodies to broad variety of Salmonella serotypes. Lipopolysaccharide(LPS) antigen extracted from Salmonella was more sensitive and specific than outer membrane protein antigen from that for detection of Salmonella antibody by using an indirect ELISA. The optimal concentration of antigen was 100ng/ml of LPS, the dilutions of conjugate and serum were 1 : 1,000~2,000 and 1 : 200~400, respectively. The mix LPS-ELISA which was used by mixing LPS from S typhimurium (group B), S choleraesuis (group C) and S enteritidis (group D) were more rapid and effective than that used LPS from individual strain for detection of Salmonella serogroup O4, O7 and O9 antibody at the same time. We could obtain the high values of optical density ($0.73{\pm}0.32$) by mix LPS-ELISA on the farm which had occurred salmonellosis, but very low values of $0.17{\pm}0.06$ on the negative farm of salmonellosis. So, the mix LPS-ELISA may be used to monitor the serological surveillance for the presence of infection with a number of serotypes of Salmonella and would be useful for prevention and control of salmonellosis in animal.

高壓下의 水熱反應에 依한 $CaO-SiO_2-H_2O$ 系 硬化體에 關한 基礎的 硏究

이희수,Lee, Hee-Soo 대한화학회 1965 대한화학회지 Vol.9 No.4

It is a fundamental study for the hardened bodies of $CaO-SiO_2-H_2O$ system to clear up various physical properties and structures of the products, using the Seosan quartz and $Ca(OH)_2$(C. P. grade) as raw materials. Various samples were obtained by varying $CaO/SiO_2$ mole ratio (0.3∼2.1) and hydrothermal conditions ($100∼220^{\circ}C$ and 2∼14hr.) within the given limit. It was found that tobermorite phase as hydrate is contained in the hardened bodies and that the development of crystal has a great influence on the strengths and other physical properties of hardened bodies.

난황면역제를 이용한 개 주요 소화기 및 호흡기질병 방제에 관한 연구 II. 난황면역제의 실험동물 및 개에 있어서의 질병방제 효과

이희수,김종만,우승룡,정병열,조윤상,유한상,윤용덕,허원,문영식,오진식,Lee, Hee-Soo,Kim, Jong-man,Woo, Seung-ryong,Jeong, Byeong-yeal,Cho, Yun-Sang,Yoo, Han-sang,Yoon, Yong-dhuk,Huh, Won,Mun, Young-sik,Oh, Jin-sik 대한수의학회 2004 大韓獸醫學會誌 Vol.44 No.3

Immunoprophylatic effect of IgY against B. bronchispetica was proven with 100% preventive rate in mice administrated with IgY with antibody titer 1:640~1:2,560. Intramuscular administration was more efficient than oral administration. This phenomenon was also observed in the therapeutic effects of IgY after challenge with B. bronchseptica in mice. In the field trials with the egg yolk antibodies from hens immunized with combined antigens with B. bronchiseptica and parvovirus, curing rates in dogs with severe clnical signs such as bloody diarrhea were 81.6% and 86.7% by intramuscular or subcutaneous administration of IgY, respectively. Safety of the antibodies in dogs was proven without any side effects such as vomiting, edema, fever, etc. by adminstration of double doses for 7 days. These results indicated that the egg yolk antibodies could be used as effective prevention and treatment of alimentary and respiratory diseases in dogs.

국내 분리주 Salmonella gallinarum의 닭에 대한 병원성

이희수,김순재,김기석,모인필,김태종,Lee, Hee-soo,Kim, Soon-jae,Kim, Ki-seuk,Mo, In-pil,Kim, Tae-jong 대한수의학회 1997 大韓獸醫學會誌 Vol.37 No.3

Fowl typhoid(FT) caused by Salmonella gallinarum is an infectious, egg-transmitted disease and characterized by swollen bronze liver, greenish-yellow diarrhea and high mortality in growing and adult chickens. Since 1992 the outbreak of FT has been increased. Several problems have been occurred such as absence of appropriate vaccines and lack of useful therapeutic methods. In these studies we investigated the pathogenicity of S gallinarum isolated in chickens. To compare the pathogenicity among the species of chickens, all chickens were challenged intramuscularly or orally with $1{\times}10^7$ CFU of S gallinarum. The brown-colored layers were more susceptible and white leghone chickens were more resistant than other species. In the brown layer chickens orally challenged, lethal doses ($LD_{50}$) of the isolates were inoculated at 1 day, 2 weeks, 4 weeks and 8 weeks old chickens with amount of $10^{4.2}$, $10^{4.7}$, $10^{7.0}$ and $10^{7.6}$ CFU, respectively. The chickens which were intramuscularly challenged with the less amount than $10^2$ CFU showed higher mortality than that of the chickens orally inoculated with same dose. Also, we investigated the recovery rates of bacteria from various organs of survival chickens which were challenged orally with $5{\times}l0^7$ CFU of S gallinarum. The bacteria was more frequently and isolated earlier from the liver and spleen than from any other ogans. In the pathogenicity test, the white-leghorn chickens which were known as resistant-strain against Salmonella were artificially immunosuppressed using bursectomy and/or dexamethasone treatment. Mortality of chickens with both bursectomized and treated with dexamethasone was higher(90%) than that of the control group(10%), the bursectomized chickens(10%) and the dexamethasone only treated group(20%). It was suggested that the protective mechanism in chickens against S gallinarum may be required both the functions of B-cells and T-cells.

난황면역제를 이용한 개 주요 소화기 및 호흡기질병 방제에 관한 연구 I. 개 보데텔라, 파보바이러스 및 개디스템퍼 항원의 닭에서의 면역반응

이희수,김종만,우승룡,정병열,조윤상,탁동섭,임숙경,유한상,윤용덕,허원,문영식,오진식,Lee, Hee-soo,Kim, Jong-man,Woo, Seng-ryong,Jung, Byeong-yeal,Cho, Yun-Sang,Tark, Dong-seob,Lim, Sook-kyoung,Yoo, Han sang,Yoon, Yong-dhuk,Huh, Woo,Mun, Y 대한수의학회 2004 大韓獸醫學會誌 Vol.44 No.1

This study was carried out to produce IgY against B. bronchiseptica, parvovirus and distemper virus that are major pathogens in alimentary and/or respiratory diseases of dogs. In the comparison of adjuvants, ISA70 was the best in the rapid induction and maintence of antibody titers. Agglutination antibody titers against B. bronchiseptica were 1:1,280 ~ 1:10,240 in sera and 1:160 ~ 1:1,280 in egg yolk. Hemagglutination inhibition(HI) titers against parvovirus in sera and egg yolk were 1:80 ~ 1:320 and 1:64 ~ 1:256, respectively. Virus neutralization titers against canine distemper was 1:8 ~ 1:64 in sera and egg yolk. These results suggested that egg yolk antibody titers could be variable according to a sort of adjuvant and antigens of the pathogens.

금융시장 전염 동적 검정

이희수,김태윤,Lee, Hee Soo,Kim, Tae Yoon 한국통계학회 2016 응용통계연구 Vol.29 No.1

본 연구에서는 금융시장 통합화에 따른 금융 시장 전염을 생물학적 전염개념에 기초하여 분석하는 검정 방법론을 제시하였다. 금융 시장 통합화를 측정하기 위하여 U-통계량을 사용하였고, 금융 시장 전염 검정을 위하여 단일방정식 오차수정 모형을 중심으로 잠재 요인모형, 분위수 회귀모형과 런검정을 사용하였다. 시뮬레이션결과 단일방정식 오차수정 모형이 자기상관을 갖는 오차항을 포함한 선형 회귀모형에서 비교적 높은 수준의 적합도를 일관성 있게 보여 주고 있다. We propose methodology to analyze the dynamic mechanisms of financial market contagion under market integration using a biological contagion analytical approach. We employ U-statistic to measure market integration, and a dynamic model based on an error correction mechanism (single equation error correction model) and latent factor model to examine market contagion. We also use quantile regression and Wald-Wolfowitz runs test to test market contagion. This methodology is designed to effectively handle heteroscedasticity and correlated errors. Our simulation results show that the single equation error correction model fits well with the linear regression model with a stationary predictor and correlated errors.

Salmonella Typhimurium의 돼지 호중구내 연속노출에 따른 특성변화

이희수,김애란,윤민,이지연,임숙경,강호영,유한상,박중원,위성환,정석찬,Lee, Hee-Soo,Kim, Aeran,Youn, Min,Lee, Ji-Youn,Lim, Suk-Kyung,Kang, Ho-Young,Yoo, Han Sang,Park, Jung-Won,Wee, Sung-Hwan,Jung, Suk-Chan 대한수의학회 2013 大韓獸醫學會誌 Vol.53 No.1

To develop a live vaccine candidate using an attenuated strain of Salmonella Typhimurium (ST), biochemical properties, plasmid profile, PFGE patterns and pathogenic analysis of the ST isolate were carried out after sequential passage of the ST isolate in porcine neutrophils. By the passage, the ability of the neutrophil-adapted isolate to utilize d-xylose was lost, while the ability of the strain to ferment trehalose was delayed after 2 or more days of the culture. Also, changes including deletion of the gene fragments were observed in PFGE analysis of the neutrophil-adapted isolates. Two plasmids, 105kb and 50kb, were cured in the strain passaged over 15 times in porcine neutrophils. The 50% of lethal dose ($LD_{50}$) of the parent strain was changed from $1{\times}10^5\;LD_{50}$ to $6{\times}10^6\;LD_{50}$ by the passage in intraperitoneal injection of the strains into mice. These results suggested that bacterial genotypic and phenotypic responses might be globally altered depending on the inside environment of neutrophils.

살모넬라 세포외막단백질 혼합백신을 이용한 돼지 및 닭에서의 살모넬라균감염증 방어효과

이희수,임숙경,조윤상,주이석,김재학,김종만,Lee, Hee-Soo,Lim, Suk-Kyung,Cho, Yun-Sang,Joo, Yi-Seok,Kim, Jae-Hak,Kim, Jong-Man 대한수의학회 2007 大韓獸醫學會誌 Vol.47 No.2

We investigated the safety, immunogenicity and protectivity of mix-crude outer membrane protein (cOMP) vaccine against salmonellosis in animals. The mix-cOMP vaccine was extracted from Salmonella enterica serovar Typhimurium (ST) and Salmonella enterica serovar Enteritidis (SE) and Salmonella enterica serovar Braenderup (SB) isolated from pigs. The mix-cOMP vaccine gave significantly higher antibody response than ST-bacterin and ST-cOMP vaccine in guinea pigs. The survival rates of mix-cOMP vaccinated groups showed significantly higher (100%) than those (0-20%) of unvaccinated control group, challenged with 3 species of Salmonella (ST, SE and SB) in mice. Vaccinated groups in pigs showed reduction of clinical signs, increase of average weight gains, decrease of bacterial recovery rates, compared with unvaccinated groups. Especially, the survival rates (100%) of vaccinated groups in chickens showed higher than that (0%) of unvaccinated group. Based on these results, we suggest that the mix-cOMP Salmonella vaccine developed in this study will be effective for the protection against Salmonellosis caused by the various serotypes Salmonella species in animals.

Salmonella gallinarum 분리주로부터 추출한 세포외막 단백질의 닭에 대한 면역원성

이희수,김수재,김기석,모인필,우용구,권용국,김태종,Lee, Hee-soo,Kim, Soon-jae,Kim, Ki-seuk,Mo, In-pil,Woo, Yong-ku,Kwon, Yong-kuk,Kim, Tae-jong 대한수의학회 1997 大韓獸醫學會誌 Vol.37 No.3

Fowl typhoid caused by Salmonella gallinarum has increased dramatically since 1992 and has caused a great economic losses in chicken industry by characterizing with high mortality. In these studies, we investigated the immunogenicity and protectivity in chickens which were immunized with outer membrane protein(OMP) extracted from isolates of S gallinarum against challenge with live microorganism. Outer membrane proteins were composed of various sizes of molecular weight including 14K, 22K, 31K, 36K, 40K and 55K and the most of them responded strongly against rabbit antisera in immunoblot analysis. The chickens vaccinated with OMP or vaccinated with whole-cell combined with OMP($200{\mu}g$/chickens) complex showed higher delayed type hypersensitivity(DTH) response than that of whole-cell vaccinated group. The protective rates of OMP or whole-cell combined with OMP complex group against challenge of S gallinarum were higher (above 75%) than those (45~50%) of whole-cell vaccinated group. All vaccines were safe and the body weight-gains of all vaccinated groups were not significantly different (p<0.05) from those of nonvaccinated control group. In vitro tests, OMP stimulated both the proliferation of lymphocytes and T-lymphocytes, and OMP-induced lymphocyte proliferation was higher in the cells of the immunized chickens with OMP than in those from the control chickens.

< 구두-A-05 > 온열욕 겸용 숯가마에서 제조된 제탄의 계절별 특성 분석

이희수 ( Hee-soo Lee ),김남훈 ( Nam-hun Kim ) 한국목재공학회 2019 한국목재공학회 학술발표논문집 Vol.2019 No.1

현재 국내 숯 산업은 높은 해외 숯에 대한 수입 의존도와 재료비 및 물가 상승, 노동인력 부족 등으로 인한 경쟁력 부족으로 위기를 맞고 있다. 이렇듯 경쟁력을 잃은 국내 숯 산업의 대안으로 숯 생산과 온열욕을 겸하는 산업이 제안되고 있다. 그러나 온열욕을 겸하기 위해 온열욕의 기능이 없는 전통 숯가마를 이용하는 것은 공중 보건과 안전성 및 쾌적성에 대한 문제점을 지니고 있다. 이에 따라 본 연구에서는 저자 등이 개발한 온열욕 겸용 숯가마에서 생산된 제탄과 전통 숯가마에서 생산된 제탄의 물리학적 특성 관찰 및 공업분석을 통한 품질 평가를 수행하여 제탄의 성능을 분석한 후 비교하였다. 또한 온열욕 겸용 숯가마에서 2-3개월 간 기간을 두고 생산된 계절별 제탄의 성능을 검토하고 분석하기 위해 밀도, 발열량, pH, 정련도 및 경도 등 물리학적 특성과 함수율, 회분, 휘발분을 통해 산출한 고정탄소 값 등의 공업분석을 통한 제탄 성능 조사 결과 값들을 비교하였다. 제탄 성능 분석을 위해 2017년 12월, 2018년 3월, 5월, 7월, 9월 및 11월, 2019년 1월에 제조된 온열욕 겸용 숯가마 제탄을 공시재료로 사용하였다. 그 결과 온열욕 겸용 숯가마에서 제조된 제탄과 전통 숯가마에서 생산 된 제탄은 서로 비슷한 품질을 보였으며 목재 제품 품질 기준에 충족하였다. 온열욕 겸용 숯가마에서 제조 된 제탄의 밀도, 발열량, pH, 정련도 및 경도 등의 물리학적 특성과 공업분석을 통해 조사한 함수율, 회분, 휘발분 및 고정탄소를 비교한 결과, 모두 KS 목재제품 품질 기준을 만족하였으며 우수한 제탄 성능을 나타내었다.