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남성 알코올 의존 환자의 삶의 질과 정신-사회-영적 특성과의 관계
최삼욱,나란희,김한오,최성빈,최영숙 大韓神經精神醫學會 2006 신경정신의학 Vol.45 No.5
Objectives : This study was designed to evaluate the relationship between quality of life (QOL) and psycho-socio-spiritual characteristics in male patients with alcohol dependence. Methods : The sample consisted of 109 men with alcohol dependence defined by DSM-IV criteria. We assessed QOL by the WHO QOL assessment instrument-BREF (WHOQOL) and SmithKline Beecham QOL (SBQOL). Sociodemographic and alcohol related data were collected, and 7 questionnaires were administered : MAST, BDI, STAI, Drinker Inventory of Consequences (DrInC), Scale of Social Support (SSS), Religious Beliefs and Behaviors (RBB) and Spiritual Well-being Scale (SWBS). The correlations between each QOL score and other variables were examined, and stepwise multiple regression analysis was performed. Results : The WHOQOL score positively correlated with education level, SSS (support) and RBB and negatively correlated with MAST, DrInC, BDI, STAI (trait) and SSS (conflict) scores. In stepwise regression analysis, the scores on the STAl and BDI contributed to the score on the WHOOOL. The SBQOL score correlated with the income level, and negatively correlated with BDI and STAI score. STAI score was a weak predictor of SBQOL score. Conclusion : The significant predictors of QOL in patients with alcohol dependence were psychological factors such as anxiety and depression.
Lee, Sun Young,Kim, Kwonseong,Oh, Han Bin,Hong, Jongki,Kang, Dukjin Korean Society for Mass Spectrometry 2017 Mass spectrometry letters Vol.8 No.3
In clinical diagnosis, it's well known that the abnormal level of uric acid (UA) in human body is implicated in diverse human diseases, for instance, chronic heart failure, gouty arthritis, diabetes, and so on. As a primary method, an isotope dilution mass spectrometry (IDMS) has been used to obtain the accurate quantity of UA in blood or serum and also develop the certificated reference material (CRM) so as to provide a SI-traceability to clinical laboratories. Due to the low solubility of UA in water, an ammonium hydroxide ($NH_4OH$) has been considered as a promising solvent to increase the solubility of UA that enables the preparation of both UA and its isotope standard solution for next IDMS-based absolute quantification. But, because of using this $NH_4OH$ solvent, it gives rise to the unwanted degradation of UA. In this study, we sought to optimize condition for the stability of UA in $NH_4OH$ solution by varying the mole ratios of UA to $NH_4OH$, followed by ID-LC-MRM analysis. In addition, we also inspected minutely the effect of the storage temperatures. Additionally, we also performed the quantitative analysis of UA in the KRISS serum certificated reference material (CRM, 111-01-02A) with diverse mixing ratios of UA to $NH_4OH$ and then compared those values to its certification value. Based on our experiments, adjusting the mole ratio of 1/2 ($UA/NH_4OH$) with the storage temperature of $-20^{\circ}C$ is an effective way to secure both the solubility and stability of UA in $NH_4OH$ solution for next IDMS-based quantification of UA in serum.
( Sun Young Lee ),( Kwonseong Kim ),( Han Bin Oh ),( Jongki Hong ),( Dukjin Kang ) 한국질량분석학회 2017 Mass spectrometry letters Vol.8 No.3
In clinical diagnosis, it’s well known that the abnormal level of uric acid (UA) in human body is implicated in diverse human diseases, for instance, chronic heart failure, gouty arthritis, diabetes, and so on. As a primary method, an isotope dilution mass spectrometry (IDMS) has been used to obtain the accurate quantity of UA in blood or serum and also develop the certificated reference material (CRM) so as to provide a SI-traceability to clinical laboratories. Due to the low solubility of UA in water, an ammonium hydroxide (NH<sub>4</sub>OH) has been considered as a promising solvent to increase the solubility of UA that enables the preparation of both UA and its isotope standard solution for next IDMS-based absolute quantification. But, because of using this NH<sub>4</sub>OH solvent, it gives rise to the unwanted degradation of UA. In this study, we sought to optimize condition for the stability of UA in NH<sub>4</sub>OH solution by varying the mole ratios of UA to NH<sub>4</sub>OH, followed by ID-LC-MRM analysis. In addition, we also inspected minutely the effect of the storage temperatures. Additionally, we also performed the quantitative analysis of UA in the KRISS serum certificated reference material (CRM, 111-01-02A) with diverse mixing ratios of UA to NH<sub>4</sub>OH and then compared those values to its certification value. Based on our experiments, adjusting the mole ratio of 1/2 (UA/NH<sub>4</sub>OH) with the storage temperature of -20℃ is an effective way to secure both the solubility and stability of UA in NH<sub>4</sub>OH solution for next IDMS-based quantification of UA in serum.
Oh, Han Bin,Leach, Franklin E.,Arungundram, Sailaja,Al-Mafraji, Kanar,Venot, Andre,Boons, Geert-Jan,Amster, I. Jonathan American Chemical Society 2011 Journal of the American Society for Mass Spectrome Vol.22 No.3
<P>The structural characterization of glycosaminoglycan (GAG) carbohydrates by mass spectrometry has been a long-standing analytical challenge due to the inherent heterogeneity of these biomolecules, specifically polydispersity, variability in sulfation, and hexuronic acid stereochemistry. Recent advances in tandem mass spectrometry methods employing threshold and electron-based ion activation have resulted in the ability to determine the location of the labile sulfate modification as well as assign the stereochemistry of hexuronic acid residues. To facilitate the analysis of complex electron detachment dissociation (EDD) spectra, principal component analysis (PCA) is employed to differentiate the hexuronic acid stereochemistry of four synthetic GAG epimers whose EDD spectra are nearly identical upon visual inspection. For comparison, PCA is also applied to infrared multiphoton dissociation spectra (IRMPD) of the examined epimers. To assess the applicability of multivariate methods in GAG mixture analysis, PCA is utilized to identify the relative content of two epimers in a binary mixture.</P>
Han Bin Oh,Sun Young Lee,Soojin Park,Yewon Lee,강혁,Ki-Ho Cho,Wha-Keun Ahn,이범구 대한화학회 2008 Bulletin of the Korean Chemical Society Vol.29 No.9
It was demonstrated that ECD MS can be successfully applied to Bovine ubiquitin (8.6 kDa, 76 amino acids) and Pseudomonas aeruginosa azurin (13.9 kDa, 128 amino acids, 1 disulfide bond) cations using photoelectrons emitted by irradiating a 266 nm UV laser light on the edge of an ion cyclotron resonance (ICR) cell trapping plate hole. The photoelectron ECD method cleaved protein backbone bonds as effectively as conventional ECD MS methods that employed a tungsten (W) wire or heated dispenser cathode as an electron source. This method offers the advantage of providing electrons for ECD MS without an apparent electron source. Key Words : 266 nm UV laser, Electron capture dissociation (ECD), Ubiquitin, Azurin, FTICR MS
Oh, Han-Bin,McLafferty, Fred W. Korean Chemical Society 2006 Bulletin of the Korean Chemical Society Vol.27 No.3
Fragmentation efficiencies of various ‘activated-ion’ electron capture dissociation (AI-ECD) methods are compared for a model system of bovine ubiquitin 7+ cations. In AI-ECD studies, sufficient internal energy was given to protein cations prior to ECD application using IR laser radiation, collisions, blackbody radiation, or in-beam collisions, in turn. The added energy was utilized in increasing the population of the precursor ions with less intra-molecular noncovalent bonds or enhancing thermal fluctuations of the protein cations. Removal of noncovalent bonds resulted in extended structures, which are ECD friendly. Under their best conditions, a variety of activation methods showed a similar effectiveness in ECD fragmentation. In terms of the number of fragmented inter-residue bonds, IR laser/blackbody infrared radiation and ‘in-beam’ activation were almost equally efficient with ~70% sequence coverage, while collisions were less productive. In particular, ‘in-beam’ activation showed an excellent effectiveness in characterizing a pre-fractionated single kind of protein species. However, its inherent procedure did not allow for isolation of the protein cations of interest.
인공지능 기법을 이용한 질량분석 스펙트럼 해석과 신종 화학물질 스크리닝 기법
오한빈(Han Bin Oh) 환경독성보건학회 2021 한국독성학회 심포지움 및 학술발표회 Vol.2021 No.5
우리는 새로운 물질들이 수없이 합성되고 있는 세상에 살고 있습니다. 환경이나 제품에 쓰이는 화학물질들을 분석하는데 있어서 새롭게 합성되는 물질은 정성 분석이 매우 어렵습니다. 새로운 물질을 정성 분석할 때에 주로 쓰이는 방법은 환경매질이나 제품내 화합물들을 분리하고, 이들에 대한 질량분석 스펙트럼 및 탠덤 질량분석 스펙트럼을 얻어, 데이터베이스 상의 자료와 비교하는 접근법인데, 이 접근법이 새로운 물질의 경우 적용이 불가능하기 때문입니다. 이런 문제를 어떻게 풀 수 있을까 하는 노력의 일환으로, 대상이 좁혀진 화학군에 경우에 미지의 화학물에 대한 정성 분석에 인공지능기법 및 혼성 유사성 검색(Hybrid Similarity Search)이라는 알고리즘을 통해 가능함을 보여준 연구결과를 발표하고자 합니다. 본 발표에서 예로써 발기부전치료 부정 의약품과 마약류에 대한 결과를 보여 드리고자 하는데, 이 방법은 환경분석의 경우에도 마찬가지원리로 적용 가능합니다. 예를 들어, 발기부전치료제들은 비교적 비슷한 화학구조를 갖고 있습니다. 비슷한 화학구조로 인해서 그들의 탠덤 질량분석 스펙트럼들 역시 비슷한 특성을 갖고 있습니다. 하지만, 이것들이 사람들의 인지능력으로는 쉽게 규칙성을 찾기 어려운 경우가 대부분입니다. 이 문제를 해결하기 위해 많은 발기부전치료제들의 탠덤 질량분석 스펙트럼들을 머신러닝 기법을 통해 훈련시켜 소위 인공지능 모델을 만들고, 이를 미지의 물질에 대한 탠덤 질량분석 스펙트럼에 적용한 결과, 매우 정확하게 미지의 물질이 발기부전치료제인지를 확인할 수 있었습니다. 하지만, 이 정보만으로는 부족하여 미지의 물질과 가장 유사한 분자와 그 분자와의 차이를 확인하기 위해서는 기존의 유사도 검색기법을 변형한 혼성 유사도 검색 알고리즘을 채택하여 분석한 결과 미지 분자에 대한 상세한 정보를 알 수 있었습니다.