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간세포 독성과 에탄올 대사에서 추출 조건에 따른 다슬기 추출물의 효과
조경환 ( Kyoung Hwan Cho ),추호진 ( Ho Jin Choo ),서민균 ( Min Gyun Seo ),김종철 ( Jong Cheol Kim ),신유진 ( Yu Jin Shin ),류기형 ( Gi Hyung Ryu ),조희영 ( Hee Young Cho ),정치영 ( Chi-young Jeong ),하영술 ( Young-sool Hah ) 한국산업식품공학회 2017 산업 식품공학 Vol.21 No.2
Although Semisulcospira libertina is generally regarded as a supplement for the alleviation of alcohol hangover, lit-tle is known about its effects on cell metabolism. Therefore, this study was conducted to analyze the constituents of the extracts prepared using different extraction methods and to compare their biochemical properties. The amino acid contents were found to be much higher in acidic and enzymatic hydrolysates than hot water extracts from S. libertina. DPPH radical scavenging activities in acidic and enzymatic hydrolysates were higher than those of hot water extracts. Three types of S. libertina hydrolysate was added to HepG2 cells damaged by acetaminophen (AAP), after which the survival rate of HepG2 cell were measured. In addition, lactate dehydrogenase (LDH) activities in the culture media were evaluated. The survival rates of HepG2 cells were 77.0±4.3% and 81.5±1.3% at 3 h and 5h enzymatic hydrolysates, respectively. These cell survival rates were higher compared to those of the negative control group (67.8±4.3%) treated only with acetaminophen. Cellular toxicities induced by treatment with AAP were also significantly alleviated in response to treatment with the extracts of S. libertina. In addition, the activities of 2 key enzymes that metabolize ethanol, alcohol dehydrogenase and aldehyde dehydrogenase, were upregulated by 4.7- and 2.7-fold respectively in response to treatment with a 3 h enzymatic hydrolysate of S. libertina. Taken together, these results provide biochemical evidence of the method by which S. libertina exerts its biological functions, including the alleviation of alcohol hangover and the protection of liver cells against toxic insults.
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황영희,이경아,신손문,박용훈,하정옥,김춘동,이영환 영남대학교 의과대학 1992 Yeungnam University Journal of Medicine Vol.9 No.2
To evaluate the nephrotoxicity of cisplatin, serum levels of sodium, potassium, chloride, calcium, phosphorous, magnesium, BUN, creatinine and creatinine clearance were measured before and after administration of cisplatin in 18 cases of patients with malignant neoplasm. The results were as follows : 1) Serum calcium, magnesium, potassium and BUN levels were changed after cisplatin administration, but those changes were not statistically significant. 2) The mean value of creatinine clearance was not decreased significantly after treatment with cisplatin. 3) Acute renal failure was developed in one case, and four cases of hypocalcemia, hypomagnesemia were also detected after administration of cisplatin.
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최영하(Young Hah Choi),강남준(Nam Jun Kang),박경섭(Kyoung Sub Park),전희(Hee Chun),조명환(Myeong Whan Cho),엄영철(Yeong Cheol Um),류희룡(Hee Yong You) 한국원예학회 2009 원예과학기술지 Vol.27 No.1
This study was conducted to investigate the effects of fruiting methods on growth, yield and quality of ‘Koko’ and ‘Suncherry’ (grafted ‘Special’ stock) tomato (Lycopersicon esculentum var. cerasiforme Alef.) .Methods of fruiting were bee (Bombus terrestris), vibration, and 4-CPA treatment. There were not significant differences among fruiting methods in growth and yield of ‘Koko’ and ‘Suncherry/Special’ tomato. Number of seeds, flesh and juice ratio of fruit were different significantly, whereas, hardness and compressive strength of pericarp were not different significantly among fruiting methods. There were not significant differences among fruiting methods on soluble solids, acidity content, ratio of fruit reduction, and occurrence ratio of unmarketable fruit of investigated tomatoes on the 7th day after harvest. It is not easy to distinguish the difference of pericarp color with naked eye among the fruiting methods or between varieties because, L* value is low and a*, b* value is closed together at the same position of L*a*b* color chart. So, it is not supported that storage quality was decreased and pericarp color was changed dark-red in bee pollination treatment. There were not significant differences in thickness and state of pericarp observed by microscope among fruiting methods or between varieties. Therefore, it was considered that the bee fruiting method is good because fruit quality was not different significantly among fruiting methods or between varieties.
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시설의 방향이 시설내 환경과 토마토 및 참외의 생육과 수량에 미치는 영향
최영하(Young Hah Choi),강남준(Nam Jun Kang),박경섭(Kyoung Sub Park),전희(Hee Chun),조명환(Myeong Whan Cho),이시영(Si Young Lee),엄영철(Yeong Cheol Um) 한국원예학회 2008 원예과학기술지 Vol.26 No.4
This experiment was conducted to investigate the effect of greenhouse orientation on the microclimate of greenhouse and the growth and yield of tomato (Lycopersicon esculentum) cv ‘Momotaro-Yoku’ and oriental melon (Cucumis melo) cv ‘Obokggul’. The temperature in a closed greenhouse was higher in north-south orientation than that in east-west orientation because the angle of incidence became larger in north-south orientation with increase of solar altitude. The number of air changes and the variation of temperature became larger in east-west orientation than those in north-south orientation when the ventilation was set to start at 28℃. Relative humidity and soil temperature were not significantly different between the two greenhouse orientations. In tomato-raising greenhouse, the light intensity inside furrows was weaker in the early morning and the late afternoon in north-south orientation than that in east-west orientation due to shadows near plants. There was no significant difference in light intensity at midday between the greenhouse orientations. The leaf area exposed to direct sunlight was greater in north-south orientation than that in east-west orientation and the leaves of middle and low parts in north side of plants could not receive direct sunlight due to a low angle of azimuth. In north-south orientation, as ripening was promoted, early yields of tomato were obtained. It was considered that photosynthesis rate was better because temperature rose quickly early in the morning reducing the stresses induced by temperature variation and more leaves of plants can receive direct sunlight. In oriental melon-raising greenhouse, ripening time and yield were not significantly different between the greenhouse orientations because thermal stress by poor ventilation and mutual shading between plants did not occur so severely.
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하경희,안종남,주선태,박구부,박기훈,김일석,진상근,Hah Kyoung-Hee,Ahn Chong-Nam,Joo Seon-Tea,Park Gu-Boo,Park Ki-Hoon,Kim Il-Suk,Jin Sang-Keun 한국축산식품학회 2006 한국축산식품학회지 Vol.26 No.1
This study was investigated the effect of aging at low temperature of seasoned pork with Korean traditional sauces. The samples, pork loins were cut by the shape of cube ($5{\times}15{\times}5\;cm$) and porks were seasoned with Korean tradition sauce such as soy sauce base (T1), red pepper sauce base (T2), and soybean sauce base (T3) in the same proportion of meat seasonings, respectively. The seasoned samples were stored, at $1{\pm}1^{\circ}C$ during 28 days. Surface meat color of seasoned pork was affected by the kind of sauces. Lightness of seasoned pork with soy sauce was decreased with increased in periods while red pepper sauce made it more red and soybean sauce produced a light seasoned pork, Lightness of inner meat color of seasoned pork was decreased with increased aging periods, and seasoned pork with soy sauce was lower compared to other treatments. TBARS of seasoned pork with soy sauce was lower compared to other treatments, and increased TBARS in all treatments with aging period. Total plate counts were increased with aging period, and those of seasoned pork with soy sauce were lower compared to other treatments. The microbial counts of Escherichia coli levels in all treatments was higher at the first day of aging periods. However Escherichia coli levels were decreased with increasing the aging period. Lactobacilli spp. of seasoned pork with soy sauce was not increased during aging periods, whereas other treatments were increased with aging period increased. 한국 전통 장류를 이용한 양념 돈육의 저장 안정성에 관한 연구로 돼지고기의 등심을 채취하여 $5{\times}15{\times}5\;cm$ 크기로 자른 후 육을 동일한 비율의 양념액에 침지하여 $1{\pm}1^{\circ}C$에서 28일간 숙성하면서 양념육(간장소스 양념육, T1; 고추장소스 양념육, T2; 된장소스 양념육, T3)의 품질 특성을 조사하였다. 양념육의 표면 육색은 소스의 영향을 많이 받았으며 간장 소스로 양념한 돈육의 명도는 낮게, 고추장소스로 양념한 돈육의 적색도는 높게, 된장 소스로 양념한 돈육의 명도는 높게 나타났다. 심부 육색의 명도는 모든 처리구에서 숙성기간에 따라 감소하였으며, 간장 소스로 양념한 돈육에서는 낮게 나타났다. TBARS는 간장 소스로 양념한 돈육이 다른 처리구에 비해 낮게 나타났으며 전 숙성기간 동안 증가하였다. 총균수는 모든 처리구에서 숙성기간에 따라 증가하였으며 간장 소스로 양념한 처리구가 다른 처리구에 비해 전 숙성기간 동안 낮은 총균수를 나타내었다. 대장균 수는 숙성 초기 $2.7{\sim}3.27\;log_{10}CFU/g$으로 다소 높게 나타났으나 숙성기간의 증가에 따라 처리구들에서 감소하였으며, 고추장 소스로 양념한 처리구는 숙성 14일에 된장소스로 양념한 처리구는 숙성 28일에 대장균이 나타나지 않았다. 유산균 수는 간장 소스로 양념한 처리구에서 숙성기간 동안 유산균수의 증가가 나타나지 않았으며, 고추장 소스로 양념한 처리구와 된장 소스로 양념한 처리구에서는 숙성기간에 따라 증가하였다.
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수출용 진공포장 야끼부다 제품의 냉장저장 중 물리화학적, 미생물학적 및 관능적 품질 특성
김일석,진상근,하경희,류현지,박기훈,정구용,Kim Il-Suk,Jin Sang-Heun,Hah Kyoung-Hee,Lyou Hyun-Ji,Park Ki-Hoon,Chung Ku-Young 한국축산식품학회 2006 한국축산식품학회지 Vol.26 No.1
본 연구는 $4{\pm}1^{\circ}C$에서 40일 저장되는 동안 수출용 진공 포장 야끼부다 제품의 물리화학적, 미생물학적 및 관능적 품질특성의 변화를 알아보고자 실시하였다. 공시된 제품은 일본인이 선호하는 육제품으로 안심 야끼부다(T1), 등심 야끼부다(T2), 뒷다리 야끼부다(T3) 3종이다. pH는 저장기간이 경과함에 따라 높아지는 경향을 보였고, 저장 40일차에는 T2가 다른 처리구에 비해 유의적으로 높았다(p<0.05). 모든 처리구에서 TBARS와 VBN값은 저장기간이 지남에 따라 증가하였다. 저장기간 동안 보수력은 T1 $81.41{\sim}92.20%$, T2 $81.13{\sim}94.51%$, T3 $82.93{\sim}87.50%$ 범위로 나타났다. 전단가는 T3가 다른 처리구들보다 전 저장기간 동안 더 높았다. 총균, 대장균 및 유산균수는 저장 전 기간 동안 $3.0\;log_{10}CFU/g$ 이하로 나타났다. 관능 검사 결과, 모든 제품이 40일까지 5.5점 이상의 점수를 받아 전체적인 기호도는 대체로 양호하였다. 이상의 결과로 미루어 볼 때 $4{\pm}1^{\circ}C$에서 진공포장된 야끼부다의 저장성은 40일까지 유지된다고 볼 수 있다. This study was carried out to investigate the changes in physicochemical, microbiological and sensory properties on vacuum packaged Yakibuda products for Export during storage at $4{\pm}1^{\circ}C$ for 40 days. Yakibuda, Japanese favorite meat product, were produced with three different pork cuts or tenderloin (T1), loin (T2) and ham (T3). The pH of all produce was tend to increased with increase in storage period. After 40 days of storage, pH of T2 was significantly (p<0.05) higher compared to other treatments. TBARS and VBN values of all treatments were increased with increased storage period. The water holding capacity ranged $81.41{\sim}92.20%$ in T1, $81.13{\sim}94.51%$ in T2 and $82.93{\sim}87.50%$ in T3. The shear force of T3 was higher than that of other treatments during whole storage time. The number of microorganism (Total plate counts, Escherichia coli, Lactobacilli spp.) were below 3.0 $log_{10}CFU/cm^2$ during the whole storage period. In sensory evaluation, the score of overall acceptability were above 5.5 point in all products. The storage properties of vacuum packaged Yakibuda could be maintained for 40 days at $4{\pm}1^{\circ}C$ storage temperature.
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전통 양념을 이용한 소스의 냉장저장 중 pH, 산도, 단백질 분해효소 활성도 및 미생물 변화
진상근,김일석,하경희,박기훈,김인진,이제룡,Jin Sang-Keun,Kim Il-Suk,Hah Kyoung-Hee,Park Ki-Hun,Kim In-Jin,Lee Jae-Ryong 한국축산식품학회 2006 한국축산식품학회지 Vol.26 No.2
Changes of pH, acidity, pretense activity and microorganism on Korean traditional seasonings using soy sauce (T1), red pepper paste (T2) and soybean paste (T3) were investigated during storage at $1{\pm}1^{\circ}C$ for 21 days. The pH and pretense activity of soybean paste were significantly (p<0.05) higher compared to those for soy sauce and red pepper paste during storage. The acidity of red pepper paste were significantly (p<0.05) higher compared to those for soy sauce and soybean paste at 1, 7 and 14 days of storage. The total plate counts and Lactobacilli spp. of soy sauce were significantly (p<0.05) lower compared to those for soybean paste and red pepper paste during storage. The Escherichia coli of red pepper paste and soybean paste were not detected during storage. 본 연구는 간장(T1), 고추장(T2) 및 된장(T3) 소스를 제조한 후 $1{\pm}1^{\circ}C$에서 21일간 저장하는 동안 pH, 산도, 단백질 분해효소 활성도 및 미생물의 변화를 관찰하고자 수행하였다. pH는 된장 소스가 간장과 고추장 소스에 비해 현저하게 높았다. 산도는 고추장 소스가 간장과 된장 소스에 비해 저장14일까지 현저하게 높았다. 단백질 분해효소 활성도는 된장소스가 간장과 고추장 소스에 비해 현저하게 높았다. 총 균수는 간장 소스가 고추장과 된장 소스에 비해 현저하게 낮았고, 저장기간이 경과함에 따라 감소하였다. 대장균은 간장 소스가 저장 1일에 검출되었으나 저장 7일부터는 검출되지 않았고, 고추장과 된장 소스는 저장 1일부터 검출되지 않았다. 유산균수는 간장 소스가 고추장과 된장 소스에 비해 현저히 낮았고, 모든 양념에서 저장기간이 경과함에 따라 감소하였다.
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