Anisakis 幼蟲의 感梁率 및 抵抗性에 관한 硏究

孫成源,朴紅蒲,李政勳 경남대학교 환경문제연구소 1990 환경연구 Vol.12 No.-

We investigated the parasitism of an Anisakislarvae by the infection rate and the average number of parasites we also observed the temperature resistance of marine fishes which were caught in the near sea along the coast of Kyungnam province(Korea) from November, 1989 to March, 1990. And we investigated the resistance of an Anisakis larvae after seasoning them. 1. The results of the infection rate examined for 10 kinds of fishes are as follow : the infection ratio of Anisakis larvae 100% in Trichiurus lepturus, 100% in Scomber japonicus, 100% in Paeudosciaema manchurica, 100% in Seriola guinqueradiata, 100% ub Mugil cephalus, 90% in Astroconger myriaster, 80% in Limanda herzensteini, 20% in Pleuronich thus cornutus, 10% in sepiella maindroni and no Anisakis larvae among 10 Octopus rariabilis. 2. The average numbers of parasites in each fish are as follow : 36.2 in Trichiurus lepturus, 15.0 in Scomber japonicus, 47.3 in Pseudosciaema manchurica, 8.3 in Seriola gusinqueradiata, 34.0 in Mugil cephalus, 38.7 in Astroconger myriaster, 8.0 in Limanda herzensteini, 0.7 in Pleuronichthys cornutus, 0.6 in Sepiella maindroni, and O in Octopus rariabilis. 3. As for the resistance to water temperature : Anisakis larvae dead immediately in higher than 60℃ water, survived 5 minutes in 50℃ water, survived 70minutes in 40℃ water, survived 8 days in 30℃ water, survived more than 10 days an 20℃ water, survived 16 hours in -5℃ water, survived 8 hours in -10℃ water, survived 6 hours in -15℃ water, survived 2 hours and 10 minutes in -20℃ water, survived 1 hour and 45 minutes in -25℃ water. 4. The resistance of Anisakis larvae to seasonings ; The larvae survived 30 minutes in sojoo, 3 hours and 30 minutes in wasabi juice, 12 hours in soybean sauce, 14 hours in soybean paste mixed with red peppers and vinegar, 17 hours in Japanese soybean sauce, 18 hours in sesame oil, 39 hours in vinegar and 60 hours in garlic juice.

成人 男子의 身體構成에 關한 硏究(Ⅰ)

蔡鴻遠,洪相完 慶北大學校 師範大學 體育學硏究會 1987 體育學會誌 Vol.15 No.-

The subjects were 6 male active adultmen(the range of their age was 30∼47 years old), and they were freshmen members in Graduate School of Dong-A University doctorate course majoring in physical education. Body compositions were calculated by body density obtained from skinfold thickness method and underwater weighing. The purpose of this study was to fine out statistical difference between two methods with T-test, and compare correlations among those variables. The conclusions were as followings; 1. Physical Characteristics. Body height and body weight were rather higher and heavier than the common same age group's. 2. T-test between body compositions obtained from skinfold thickness method and underwater weighing method. There were not significant differences between the values of body compositions calculated from two methods(|T|<2.57, p<0.05). 3. Body Compositions. ① % far was 12.50±1.24% (skinfold thickness method) and 17.53±3.26% (underwater weighing method). ② Supposing that two subjects' body weight were same, We could find that the lighter underwater body weight the smaller body density. ③ We could assume that the values of body composition obtained from underwater weighing method were more accurate than those from skinfold thickness method. 4. Correlations. ① There were very high positive correlations between height and fat(p<0.05), Fat/Height(p<0.05), LBM/Height(p<0.01), and LBM(p<0.001). There were very high positive correlations between body weight and % fat(p<0.05), Fat/Height (p<0.01), and Fat·LBM·LBM/Height(respectively p<0.001). Besides, there were positive and negative correlations between the other values, but not statistical significances. ② There were high correlations between triceps and % Fat, % LBM. ③ There were positive correlations between body density and % Fat, but not a statistical significance. ④ There were positive correlations between vital capacity and residual lung volume % Fat, Fat, LBM, Fat/Height, and LBM/Height respectively, but not statistical significances. ⑤ Between triceps(X) and % fat(Y'), We could calculate regression equation (Y')=0.561X+8.249(Sy.x=1.039, r=0.819, p<0.05), between triceps(X) and Fat/Height(Y'), Y'=0.467X+1.628(Sy.x=1.033, r=0.821, p<0.05), between triceps(X) and % LBM(Y'), Y'=-0.561X+91.755(Sy.x=1.038, r=-0.819, p<0.05), but, in other cases there were not statistical significances because either correlation was low or Sy.x was large.

공역 리놀레산 유도체의 세균생장 억제효과

金紅出,趙鏞雲,李相元,金哲鎬 진주산업대학교 2000 산업과학기술연구소보 Vol.- No.7

Antibacterial activity of CLA-K salt was surveyed in this paper. CLA-K(10%) exhibited antibacerial activity at the concentration more than 60 ㎕. The antibacterial activity of CLA-K in liquid culture was relatively proportional to its concentration. The resistance of bacteria against CLA-K increased as time goes by. Streptococcus mutans, Bacillus cereus KCCM-l1204, Lactobacillus plantalium and Escherichia coli ATCC-11835 were relatively sensitive to CLA-K. Staphylococcus aureus KCTC-l927 and Klebsiella pneumoniae KCTC-2208 sensitively reacted to the concentration of CLA-K.

Aspergillus awamori와 Zymomonas mobilis로 구성된 고정화혼합 배양계의 에탄올 생산에 미치는 Triton, PVA 및 PEG의 영향

이상원,서권일,박석규,손봉수,김홍출,성찬기 순천대학교 기초과학연구소 1995 基礎科學硏究誌 Vol.6 No.-

The effects of triton-X 100, PVA and PEG on the ethanol production and raw starch hydrolysis by co-immobilized A. awamori and Z. mobilis (A-Z system) were investigated. When gel beads containing A. awamori(1.25 × 10 exp (9) spores/ℓ - gel) and Z. mobilis(0.5g cell/ℓ - gel) were cultured in 2% raw starch solution at 30℃ for 120 hours, ethanol concentration, ethanol yield (Y_p/s) and pH of culture broth were 2.8g/ℓ, 0.16 and 3.8~4.0, respectively. Ethanol production and starch hydrolysis by addition of PVA was efficient, compared to that of PEG and ethanol concentration in culture broth with 0.2% PVA was 3.56g/ℓ. At A-Z 36 culture system with changing silicon check valve for cotton plug at 36 hours in A-Z culture system, ethanol yield on glucose, Y_p/s = 0.36, showed 2 times higher than that of control culture system(cotton plug culture). Ethanol production and productivity by cultivating 85g gel beads at 30℃ in a 1ℓ jar fermentor(220rpm) of medium 500㎖ were 32g/ℓ and 1.8g/ℓ/day, respectively. After 12 hours of cultivation, gel beads were distinguished oxygen-rich surface for A. awamori from oxygen-deficient central part for Z. mobilis.

에이즈 환자의 거대세포바이러스 감염증

김홍빈,박상원,김남중,최희정,신동현,오명돈,김우호,정흠,최강원 대한감염학회 1998 감염 Vol.30 No.4

배 경 : 거대세포바이러스 감염은 인간면역부전바이러스 (Human Immunodeficiency Virus, HIV) 감염자에서 발생하는 가장 중요한 기회감염증의 하나이다. 특히, 국내에서는 95%이상의 국민이 거대세포바이러스의 1차감염을 경험한 상태이므로 에이즈 환자에서 거대세포바이러스 질환의 빈도가 높을 것으로 예상된다. 대상 및 방법 : 1987년 10월부터 1996년 9월까지 서울대학교 병원에서 추적 관찰하였던 128명의 HIV 감염자를 대상으로 하였다. 이들 환자의 의무 기록에서 임상 자료를 얻었다. 거대세포바이러스 망막염은 안과 전문의의 임상적인 소견으로 진단하였으며, 위장관 등 망막이외 부위의 거대세포마이러스 질환은 조직학적으로 확인된 경우에 한하였다. 결 과 : 대상환자 128명의 추적관찰기간은 중앙값이 6(0∼59)개월 이었으며 7명 (5.4%)에서 거대세포바이러스 질환이 발생하였다. 거대세포바이러스 질환은 망막염이 6예, 식도염이 2예, 대장염이 1예, 폐렴 및 부신감염이 1예, 범발성 감염이 1예였다. 거대세포바이러스 질환의 발생빈도는 CD4+ 림프구 수가 적을수록 높았으며, CD4+ 수가 200/㎣미만인 환자 중 15% (6/33)에서 거대세포바이러스 질환이 확인되었다. 11예 중 9예를 ganciclovir로 치료하였으며 이중 7예에서 호전 또는 진행의 억제가 확인되었다. 추적관찰이 가능한 6예 중 3예 (50%)에서 재발하였다. 결 론 : 국내 에이즈 환자에서 거대세포바이러스 질환은 비교적 흔한 기회감염증이다. Background : Cytomegalovirus (CMV) infection is one of the important opportunistic infections in immunocompromised patients. In Korea, seroprevalence of IgG against CMV is over 95%. Therefore, CMV diseases are expected to be a prevalent opportunistic infection in AIDS patients. in Korea. Methods : We reviewed the medical records of 128 patients with HIV infection who visited the Seoul National University Hospital during the period from Nov. 1987 TO Sep. 1996. All the patients were examined by one ophthalmologist and the diagnosis of CMV retinitis were made by funduscopic findings. Other CMV diseases were diagnosed when histopathologic examinations showed the characteristic cytomegalic cells. Results : Median duratior, of follow-up was 8 months. Eleven CMV diseases were found in 7 patients (5.4%): 6 patients ahd retinitis, 2 esophagitis, 1 colitis, 1 pneumonitis, and one patient developed disseminated infection. Fifteen percent (6/33) of the patients whose CD4+ lymphocyte counts were less than 200/㎣ at baseline developed CMV diseases. Out of the 9 cases treated with ganciclovir, 7 improved or were stabilized. No patient received maintenance treatment and 3 had relapsed. Conclusion : CMV diseases are common opportunistic infections in AIDS patients in Korea.

Opaque의 두께가 전장용 레진의 색채에 미치는 영향

박상원,조신석,양홍서 대한치과기재학회 1999 대한치과재료학회지 Vol.26 No.3

The purpose of this study was to evaluate the color difference of specimens with the variation of metal type and opaque thickness. Resin veneered specimens with the same color were fabricated and evaluated by the CIE L*a*b* system. Specimens were divided into 4 groups as follows ; Group P1; Resin with precious metal alloy, 0.1 mm opaque layer Group P2; Resin with precious metal alloy, 0.5 mm opaque layer Group B1; Resin with nonprecious metal alloy, 0.1 mm opaque layer Group B2; Resin with nonprecious metal alloy, 0.5 mm opaque layer The results obtained were as follows ; 1. L* value of group P1 was lower than that of group P2. There was no significant differences in a* values among tested group. 2. Opaque thickness did not produce any significant differences of L*, a*, b* value in the resin veneer groups on the base metal. 3. The ΔE*ab value between P1 and P2 was 2.228 which is noticeable, while the color difference between B1 and B2 was slight.

전장용 레진의 색안정성에 대한 연구

박상원,조신석,양홍서 대한치과기재학회 1999 대한치과재료학회지 Vol.26 No.3

In this study, metal specimens were made of semiprecious metal alloy and veneered with composite resin(Dentacolar, Kulzer). Germany, Color stability of each specimen was evaluated by the CIE L*a*b* system. Specimens were divided into 3 groups as follows ; Group 1; Resin with 0.1 mm opaque layer Group 2; Resin with 0.25 mm opaque layer Group 3; Resin with 0.5 mm opaque layer The purpose of this study was to evaluate the color difference and color stability of specimens, following to the opaque thickness. The results obtained were as follows ; 1. The group 1 had best color stability after thermocycling. 2. After thermocycling, the specimens with more than 0.25 mm opaque thickness showed detectable color change and ΔE*ab values were more than 3.3 level. 3. After thermocycling, L* value was decreased and a* value was increased in group 2 and 3.

청국장 발효에 적합한 미생물의 분리 및 특성

이상원,손미예,조용운,김홍출,갈상완,김철호 진주산업대학교 1999 산업과학기술연구소보 Vol.- No.6

장류발효 식품으로부터 protease 및 amylase 활성이 높은 균주를 중심으로 348 균주를 1차 분리하고, 그 분리균 중 2% skim milk가 함유된 LB평판배지(SM배지)화 2%corn starch가 함유된 LB평판배지(CM배지)에서 halo zone이 크고, 두 효소의 활성이 동시에 높은 SW-251균주를 최종 선별하였다. SW-251균주의 생육 온도 범위는 10∼60℃ 이었으며, 최적 생육온도는 35∼40℃ 이었다. 그리고 최적생육 pH는 6.5 부근이었으나 pH6.0 이하나 pH8.0 이상에서는 생육이 저하하였다. SW-251균주는 호기성의 간균 (0.8∼1.3x1.8∼2.5㎛)으로 운동성이 있으며, 그람양성 반응을 나타냈다. 10% 이하의 NaCl에 내성을 갖고있으며, starch 및 casein을 빠르게 분해하는 세균으로 Bacillus속의 특성을 갖고 있었다. Among 348 strains isolated from fermented soy foods, SW-251 strain has a potent protease and amylase activities on SM medium(LB agar plate supplemented with 2% skim milk), and CM medium(LB agar plate supplemented with 2% corm starch0, respectively. The optimal temperature and growth temperature range for cell growth were 35∼40℃ and 10∼60℃, respectively. The optimal pH was 6.5 and pH range of its growth was 6.0 and 8.0. The isolate was an aerobic bacterium (rod type, 0.8∼1.3 x 1.8∼2.5㎛), then there was its mobility, Gram staining and hydrolysis of starch and casein were positive reaction. Salt tolerance was below 10% NaCl. The strain was identified as a Bacillus sp. SW-251 with respect to morphological, cultural and physiological characteristics.

영지버섯 리큐르주의 침출시간에 따른 성분변화

金紅出,李相元,金哲鎬,趙鏞雲 진주산업대학교 2000 산업과학기술연구소보 Vol.- No.7

The physicochemical factors such as organic acid composition, pH, sugar content, acidity and color according to extraction time of Ganodema licidum liquor changed as followings; 1. The major general components of Ganodema licidum were analyzed to be crude fiber(47.5%), carbohydrate (29,4%), crude protein (14.8%), crude fat (6.5%) and crude ash (1.8%) in dry basis. 2. pH and sugar content in Ganodema licidum liquor slightly decreased, whereas acidity increased with extraction time. 3. The luminosity of color (L) showed no remarkable change. 4. The tone of color (a) decreased, whereas the chroma (b) increased as time goes by. 5. The concentration of total organic acid in Ganodema licidum liquor was 2.453%, and nine kinds of organic acids such as oxalic, citric, tartaric, succinic, lactic, formic, acetic, propionic and isobutyric acid were detected. Among them, the concentration of lactic acid was highest acetic and isobutyric acid were medium level, and that of citric, formic, succinc and tartaric acid was very low.

유량측정기법에 관한 연구 : 실험수로에서 희석법을 중심으로 Dilution Method in Experimental Channel

김성원,지홍기,이순탁 嶺南大學校 環境問題硏究所 1996 環境硏究 Vol.16 No.1

실험수로에서 희석법을 이용한 유량측정의 기법은 일정량주입법과 일시주입법으로 나누어 진다. 본 연구에서는 일정량주입법에 의한 유량측정 결과치가 일시주입법보다 좀 더 실험유량값에 근접한 것으로 분석되었고, 만약 측정장치의 설치오차, 주입용액의 완전 혼합오차와 농도 측정오차 등을 최소로 한다면, 실험수로에서의 유량측정에 있어서 보다 정확한 유량측정을 할 수 있을 것이다. 이와같은 연구분석의 결과로서, 유속계를 사용할 수 없는 하천유역이나 다른수로에서는 희석법을 이용한 유량측정기법이 적용되어 높은 정밀도를 구할 수 있다고 고려된다. It is divided into constant-rate injection method and sudden-injection method in the technique of discharge measurement using the dilution method for experimental channel. It is analyzied that the value of constant-rate injection method is approached more exact than that of sudden-injection method in this research. In this study, if it is minimized for the exact installation error of the testing equipment, the perfect mixing error of the injection-solution and concentration measurement error and so on, It is measured the more exact discharge in this experimental channel. It is considered that the dilution method is applied for the river basin which in not used the current meter and another channel in this research.