배초향으로부터 Grb2-Shc domain 결합저해 물질의 분리

이은숙,안병태,이새봄,김혜경,복성해,정태숙,Lee, Eun-Sook,Ahn, Byung-Tae,Lee, Sae-Bom,Kim, Hyae-Kyeong,Bok, Song-Hae,Jeong, Tae-Sook 한국생약학회 1999 생약학회지 Vol.30 No.4

SH2 domains and their associated catalytic or noncatalytic proteins constitute critical signal transduction targets for drug discovery. Grb2 associates with phosphotyrosine sites of the activated receptors or Shc via their SH2 domain to link receptor tyrosine kinases to ras signalling. Blocking of the Grb2-Shc complex may be to intervene the oncogenic signal transduction pathways and to develop a new antitumor drug. In the search for blockers of Grb2 SH2-Shc interaction, Lutein, a family of carotenoids, was isolated from the extract of the leaf of Agastache rugosa O. Kuntze as SH2 domain antagonists. The $IC_{50}$ of Lutein against Grb2-Shc binding was $6.8\;{\mu}M$.

Locoregional Recurrence of Breast Conserving Surgery after Preoperative Chemotherapy in Korean Women with Locally Advanced Breast Cancer

이은숙,Seok Won Kim,김석기,Keun Seok Lee,Eun-A Kim,Youngmee Kwon,Kyung Hwan Shin,강한성,노정실,Eun Sook Lee 한국유방암학회 2011 Journal of breast cancer Vol.14 No.4

Purpose: Preoperative chemotherapy has been used to increase the rate of breast conserving surgery (BCS) in Caucasian women. However, whether it would also increase the rate of BCS in Korean women has not been verified. The aim of this study was to determine the effectiveness of preoperative chemotherapy to make BCS possible in Korean women who have locally advanced cancer without any increase of locoregional recurrence according to operation methods (BCS vs. mastectomy). Methods: From August 2002 to April 2005, 205 patients with stage II or III breast cancer were enrolled in a phase III randomized trial of preoperative chemotherapy. Surgeons decided on the type of surgery (mastectomy or BCS) at initial diagnosis. By randomization, patients received four cycles of either docetaxel/capecitabine or doxorubicin/cyclophosphamide followed by surgery and crossover to the other treatment as postoperative chemotherapy. Results: The mean tumor size was 3.29 cm and the mean breast volume was 489 cc at diagnosis. After preoperative chemotherapy, clinical response was shown in 76.0% of the patients. Of the 71 patients planned for a mastectomy at initial diagnosis, 27 patients underwent BCS (38.0%). Clinical T stage after preoperative chemotherapy, pathologic T size and lymphatic invasion were correlated with conversion to BCS. In multivariate analysis, only lymphatic invasion showed statistical significance. Locoregional disease-free survival did not statistically differ between the two operation methods for the patients who were planned for a mastectomy at the initial exam. Conclusion: This study showed that preoperative chemotherapy also increased the rate of BCS, while avoiding any increase of locoregional recurrence in Korean women with locally advanced breast cancer.

유아의 구강건강관리 실태와 유치우식에 관한 연구

이은숙,신승철 ( Eun Sook Lee,Seung Chul Shin ) 대한예방치과·구강보건학회 1996 大韓口腔保健學會誌 Vol.20 No.3

논문편 : 영국 군복에 관한 연구 - 고대와 중세시대를 중심으로 -

이은숙,박재옥 ( Eun Sook Lee,Jae Ok Park ) 대한가정학회 1996 Human Ecology Research(HER) Vol.34 No.4

상악동 병변의 인지시 파노라마 방사선사진의 유용성

이은숙,박창서,Lee Eun-Sook,Park Chang-Seo 대한영상치의학회 1999 Imaging Science in Dentistry Vol.29 No.1

Purpose: to evaluate the usefulness of the panoramic radiography, generally used in dental clinic, for the detection of the maxillary sinus pathosis due to the inflammation and tumor, through the comparison with CT findings as the absolute standard. Materials & Methods: 150 maxillary sinuses of 75 patients with or without particular signs and symptoms and showing soft tissue lesions or destruction of maxillary sinus walls in at least one sinus in CT, were selected as subject samples, and the panoramic radiography of the same patients were interpretated by 3 dental radiologists and 3 non dental radiologist given no previous informations. Using the CT findings as the absolute standard, the diagnostic ability of panoraimc radiography in the mucosal thickening, maxillary sinus haziness, inferior wall destruction, medial wall destruction, posterolateral wall destruction and the superior wall destruction was evaluated using the ROC curve and the difference between dental radiologist group and non dental radiologist group was also evaluated. Results: 1. When dental radiologist group interpretated the destruction of inferior wall and posterolateral wall, the kappa value which shows interobserver's coincidence was above 0.75. 2. The diagnostic ability according to site of interpretation was the highest when the inferior wall was interpretated in both observer groups and there was a statistically significant difference between the dental radiologist group and non dental radiologist group in interpretating the mucosal thickening, haziness, destruction of the inferior and medial wall(p<0.05). 3. The diagnostic ability in detecting the destruction of the sinus walls was better than in soft tissue lesions in both groups and between the groups there was a statistically significant difference(p<0.05). Conclusion: When detecting the destruction of inferior and posterolateral wall of the maxillary sinus there was coincident with that of CT findings, and so it is considered that diagnostic ability of panoramic radiography is high in this point. But in interpretating the destruction of medial wall and soft tissue lesions, diagnostic ability of the panoramic radiography was relatively low and there was a significant difference between the dental radiologist group and non dental radiologist group. Therefore CT or other auxiliary diagnostic method will be necessary when evaluating thorough pathosis of maxillary sinus objectively.

체외수정 과배란 유도에서 hCG 주사 당일의 혈청 Progesterone과 Estradiol 농도가 수정율 및 임신율에 미치는 영향에 관한 연구

이은숙,이상훈,배도환,Lee, Eun-Sook,Lee, Sang-Hoon,Bae, Do-Hwan 대한생식의학회 1996 Clinical and Experimental Reproductive Medicine Vol.23 No.1

체외수정 시술을 위해 중앙대학교부속병원 산부인과학 교실 불임크리닉을 방문한 환자중 1993년 3월부터 1994년 8월까지 난관폐쇄로 인하여 불임이 된 환자 113명(119주기)을 대상으로 GnRH-a 병합요법 중 Short protocol 방법으로 과배란시 임신된 45명(47주기)과 임신이 되지 않은 68명(72주기)에서 hCG 투여 당일 혈청내 E2 및 P4 수치를 측정하여 임신의 결과를 비교하였다. 1. 환자의 평균 연령 및 불임기간은 임신군에서 $33.2{\pm}14.8$세 및 $4.2{\pm}3.4$년이었으며 비 임신군에서는 $34.5{\pm}21.7$세 및 $3.9{\pm}2.8$년으로 연령 및 불임기간의 차이는 없었다. 2. hGC 투여 당일 측정한 혈중 E2치는 임신군에서는 $1643{\pm}987.9$ pg/ml, 비임신군에서는 $1367{\pm}875.8$ pg/ml로 임신군에서 유의하게 높았다(P<0.01). 또한 혈중 LH치는 인신군 에서는 $16.7{\pm}10.4$ ng/ml, 비임신군에서는 $18.3{\pm}8.3$ ng/ml로 임신군에서 유의하게 낮았다 (P<0.01). 혈중 P4치는 임신군에서는 $1.0{\pm}0.7$ ng/ml이었고 비임신군에 서는 $2.1{\pm}1.4$ ng/ml로서 임신군에서 유의하게 낮았다(P<0.001). 3. hCG 투여당일 E2/P4 비는 임신군에서 $1865.6{\pm}318.1$, 비임신군에서는 $1324{\pm}377.7$ 로서 유의한 차이가 있었다(P<0.01)(Table 3). 4. 주기당 수정율은 임신군에서 $61.3{\pm}21.3%$, 비임신군에서는 $41.1{\pm}20.3%$로 임신군에서 의미있게 높았고(P<0.01) 이식된 배아의 수는 임신군 47주기에서 $4.2{\pm}2.2$개, 비임신군 72주기에서는 $2.3{\pm}1.2$개로 두 군간의 차이는 없었다(Table 4). 이상에서 임신이 된 군은 임신이 안된 군보다 혈중 progesterone 치가 의미있게 낮았고 혈중 estradiol 치는 의미있게 높았음을 알 수 있었다. 혈중 progesterone 및 estradiol치는 과배란 유도 후 체외수정시술에 있어서 수정율 및 임신율에 영향을 줄 수 있다고 생각되며 이는 체외수정시술시 과배란후 임신의 예후판정에 효용성이 있을것으로 사료된다. Controlled Ovarian hyperstimulation(COH) is generally used to obtain synchronous high quality oocytes in in vitro fertilization-embryo transfer(IVF-ET). Many investigators have studied the relationship between serum hormone levels and outcomes of IVF-ET because there is no accurate estimation method of oocyte quality. Early premature luteinization of follicles before oocyte retrieval is the most troublesome problem in COH for IVF-ET. Gonadotropin-releasing hormone agonists(GnRH-a) are used as adjuncts with gonadotropins for COH in patients undergoing in IVF. The possible benefits of GnRH-a pretreatment include improving oocyte quality, allowing a more synchronous cohort of follicles to be recruited, and preventing premature lueinization hormone surges. In COH of IVF cycles, we investigated whether an elevated progesterone(P4) level on the day of human chorionic gonadotropin(hCG) administration indicates premature luteinization and is associated with a lower fertilization rate. Many investigators have studied that the lower fertilization rates seen in patients with elevated P4 levels might result from an adverse effect of P4 on the oocytes. We hypothesizes that serum P4 levels around the day of hCG may be helpful prediction of out come in IVF-ET cycles. Success rates after COH of IVF-ET cycles are dependent upon many variable factors. Follicular factors including the number of follicles, follicular diameters and especially serum estradiol(E2) levels as an indirect measurement of follicular function and guality have been thought to influence the outcomes of IVF-ET. To assess whether serum P4 and E2 levels affect the fertilization and pregnancy rate, we reviewed the stimulation cycles of 113 patients (119 cycles) undergoing IVF-ET with short protocol with GnRH-a, from March 1993 to August 1994 retrospectively. The serum P4 and E2 levels were compared on the day of hCG in the pregnant group, 45 patients(47 cycles) and in the non-pregnant group, 68 patients (72 cycles) respectively. The serum E2 level in non-pregnant group was $1367{\pm}875.8$ pg/ml which was significantly lower than that of pregnant group, $1643{\pm}987.9$ pg/ml( p< 0.01 ). And the serum P4 level in non-pregnant group was $2.1{\pm}1.4$ ng/ml which was significantly higher than that of pregnant group, $1.0{\pm}0.7$ ng/ml( p< 0.001 ). The fertilization rate was $61.3{\pm}21.3%$ in pregnant group which was higher than that of non-pregnant group, $41.1{\pm}20.2%$ (p< 0.01). We suggest that the serum levels of P4 and E2 on the day of hCG administration are additional parameters that predict the outcomes of IVF-ET cycles.

측두하악관절내장증에서 하악과두운동과 측두하악과절조영상의 상호관계

이은숙,유동수,박태원,최순철,Lee Eun-Sook,You Dong-Soo,Park Tae-Won,Choi Soon-Chul 대한영상치의학회 1994 Imaging Science in Dentistry Vol.24 No.2

Arthrography of the temporomandibular joint is a useful method of demonstrating the soft tissue abnormalities related to disc dysfunction. In this study, 19 asymptomatic joints, 31 joints with anterior disc displacement with reduction, 31 joints with anterior disc displacement without reduction which were classified by arthrotomography under fluoroscopy were evaluated to determine the linear measurement of anterior recess of inferior joint space and the relationship between the condylar anterior translation and the severity of the internal derangements. Their fluoroscopic images were also evaluated to describe the characteristics of condylar paths in internal derangements of the temporomandibular joints. The results were as follows; 1. The mean lengths of the anterior recess in asymptomaic group. reduction group. and non-reduction group were 8.7±1.6㎜. 11.2±1.7㎜, 12.8±1.7㎜ respectively. The length of the anterior recess was increased according to the severity of the internal derangements(P<0.05). 2. Linear measurements of anterior movement of condyle on maximum mouth opening were 13.1 ±4.2㎜, 15.9±4.1㎜, 5.0±3.7㎜ in asymptomatic group. reduction group, and non-reduction group respectively. Compared with asymptomatic group, reduction group showed hypermobolity of the condyle and non-reduction group showed hypomobility. 3. Condyles moved beyond the crest of articular eminence in 80% of reduction group and did not reach it in 70% of non-reduction group. 4. The condyle moved mainly superiorly in reduction group(66%) and horizontally in asymptomatic group(47%). There were no cases to move superiorly in non-reduction group.

논문편 : 소비자의 자아조정 수준에 따른 상황별 의복행동 연구

이은숙,박재옥 ( Eun Sook Lee,Jae Ok Park ) 대한가정학회 1997 Family and Environment Research Vol.35 No.6

Portulaca oleracea L. 추출물에 의한 Escherichia coli KCTC 2441의 생육억제

이은숙,서부일,Lee, Eun-Sook,Seo, Bu-Il 대한한의학방제학회 2001 大韓韓醫學方劑學會誌 Vol.9 No.1

쇠비름 추출물의 농도가 1%정도부터 Escherichia coli KCTC 2441의 성장 억제 효과가 나타났으며 7%일 때 균의 성장이 급속도로 억제되었다. 즉 쇠비름 추출물이 Escherichia coli KCTC 2441에 대하여 항균작용을 나타내고 있음이 증명되었다. 6월에 양지 바른 곳에서 자란 쇠비름이 대장균 성장 억제 효과가 높았으며 잎보다는 줄기 부분이 잎 중에서도 적자주색을 띠는 잎이 대장균 성장 억제 효과가 높았다. This study was performed to investigate the effects of purslane extracts on growth inhibition of Escherichia coli KCTC 2441. The purslane extract is effective on retarding growth of Escherichia coli KCTC 2441 and effect of its extract was founded from 1% concentration. At 7% the growth of Escherichia coli KCTC 2441 was suddenly retarded. The stem of purslane in a sunny place on June is more effective than its leaf. Leaves with a red violet color has high effect on retarding growth of Escherichia coli KCTC 2441. These results suggest that purslane extract is effective in growth inhibition of Escherichia coli KCTC 2441.

폴리에틸렌 랩과 에어캡의 적용이 미숙아의 체온 유지에 미치는 효과

이은숙,이한나,박지현,Lee, Eun Sook,Lee, Han Na,Park, Ji Hyun 병원간호사회 2020 임상간호연구 Vol.26 No.2

Purpose: This study was an quasi-experimental study to compare the effect of applying polyethylene wrap and aircap in maintaining body temperature of preterm infants. Methods: The participants were 51 preterm infants. Aircap was applied to the experimental group (n=23) and polyethylene wrap was applied to the control group (n=28) when the preterm infants admitted to neonatal intensive care unit. The data was collected at W hospital in J-province from June 2016 to May 2017. A total of 9 body temperature measurements were taken at 3 hours interval from 5 min to 24 hours after admission. Repeated measure ANOVA, independent t-test and χ² test were conducted used with SPSS/WIN 24.0 Results: There were no significant difference in the homogeneity tests for general characteristics and dependent variables prior to the experiments (t=0.57, p=.566). There was a significant difference on body temperature of preterm infants over time (F=3.24, p=.020). There was no significant difference on body temperature between polyethylene wrap and aircap application groups (F=1.29, p=.261). The interaction between the group and the time was insignificant (F=1.51, p=.214). Conclusion: The findings demonstrated that both methods of applying polyethylene wrap and aircap on the body in preterm infants had effect in maintaining body temperature.