여중생의 월경전증후군과 우울에 관한 서술적 조사연구

최고은,이경은,양지은,안수빈,김영우,최슬기,최혜준,김나현,간보선 이화여자대학교 간호과학대학 2015 이화간호학회지 Vol.- No.49

Objectives : The purpose of this research is to analyze the frequency, clinical characteristics of premenstrual syndrome, and the relationship between premenstrual syndrome and depression in middle school girls. Methods : Subjects of the research were 241 students recruited from 3 women's middle schools in Seoul, Korea. The questionnaires were composed of clinical characteristics of menstruation, Premenstrual Symptom Screening Tool, and Center for Epic Studies Depression Scale. Data were statistically analyzed by Chi-square, t-test, ANOVA, and Scheffe test. Results : The proportion of no/mild premenstrual syndrome was 83.4%, moderate to severe premenstrual syndrome was 12.9% and premenstrual dysphoric disorder was 3.7%. Premenstrual syndrome proportion was higher in severe menstruation pain group(χ2=11.956, p=.008) and depression group (χ2=50.85, p<.001). The mean of depression score was 11.61±9.09. Menstrual pain (F=4.59, p=.004) and premenstrual syndrome (F=68.81, p<.001) were found to be significant in depression. Conclusions : We identified that 16.6% of middle school girls suffered from premenstrual syndrome. We also analyzed that the clinical characteristics of menstruation pain and depression were related to premenstrual syndrome. And there was a relationship between depression and premenstrual syndrome. Accordingly, we can think of applying nursing interventions on menstruation pain and depression in middle school girls who are undergoing premenstrual syndrome.

Association of Extracellular Cleavage of E-Cadherin Mediated by MMP-7 with HGF-Induced in vitro Invasion in Human Stomach Cancer Cells

Lee, K.H.,Choi, E.Y.,Hyun, M.S.,Jang, B.I.,Kim, T.N.,Kim, S.W.,Song, S.K.,Kim, J.H.,Kim, J.-R. S. Karger AG 2007 European surgical research Vol.39 No.4

<P><I>Background:</I> Proteolytic shedding of the ectodomain of a variety of transmembrane proteins, including cell-to-cell adhesion molecules, has been observed in solid cancers. We have investigated whether extracellular cleavage of E-cadherin mediated by matrix metalloproteinase-7 (MMP-7) is involved in hepatocyte growth factor (HGF) induced in vitro invasion in stomach cancer cells. <I>Methods:</I> The effects of HGF on the expression of E-cadherin/β-catenin and MMP-7 at both the protein and mRNA levels were assessed in stomach cancer cells, NUGC-3 and MKN-28, and in cells in which the expression of MMP-7 was downregulated by transfection with a MMP-7 short hairpin RNA plasmid. <I>Results:</I> Treatment with HGF increased the extracellular cleavage of E-cadherin and the release of MMP-7 and reduced the level of E-cadherin in a dose- and time-dependent manner. HGF treatment repressed the phosphorylation of β-catenin in a Triton-soluble fraction, but enhanced this phosphorylation in a Triton-insoluble fraction. The association of E-cadherin with β-catenin was decreased by HGF treatment in the Triton-soluble fraction. In addition, treatment of MMP-7 short hairpin RNA transfected NUGC-3 cells with HGF resulted in no extracellular cleavage of E-cadherin and also decreased the in vitro cell invasion. <I>Conclusions:</I> These results suggest that incubation with HGF mediated the release of MMP-7, resulting in extracellular cleavage of E-cadherin from stomach cancer cells. This might be a key mechanism in HGF-induced in vitro invasion and metastasis.</P><P>Copyright © 2007 S. Karger AG, Basel</P>

Synthesis and characterization of a wide bandgap polymer based on a weak donor-weak acceptor structure for dual applications in organic solar cells and organic photodetectors

Choi, E.Y.,Eom, S.H.,Song, C.E.,Nam, S.Y.,Lee, J.,Woo, H.Y.,Jung, I.H.,Yoon, S.C.,Lee, C. Elsevier Science 2017 Organic Electronics Vol.46 No.-

We synthesized a novel wide bandgap polymer, PDTFBT, forming a weak donor (WD)-weak acceptor (WA) structure for use in organic photodetectors (OPDs) and organic solar cells (OSCs). The fluorination in the D unit and the alkoxy substitution in the A unit induced WD and WA properties, respectively. The WD-WA structure of PDTFBT effectively broadened the bandgap compared to typical D-A structures, and the S-F and S-O dipole-dipole interactions induces a highly planar backbone structure with excellent π-π stacking in the vertical direction. In OPDs, conformationally less disordered PDTFBT polymer retained the constant responsivity and significantly improved the detectivity of PDTFBT:PC<SUB>71</SUB>BM devices even with a thick active layer of 470 nm, contrary to the variation in the responsivity of P3HT:PC<SUB>61</SUB>BM devices depending on the thickness. In OSCs, the deep HOMO energy level (-5.57 eV) of PDTFBT led to high Voc of 0.92 V in PDTFBT:PC<SUB>71</SUB>BM devices, which was 0.3 eV higher than that of P3HT:PC<SUB>61</SUB>BM devices (0.62 V), resulting in 1.8-fold enhanced power conversion efficiency. We demonstrated that the WD-WA structure with S-F and S-O interactions is highly promising strategy to make wide bandgap polymers for organic photodetectors and for the bottom cell of tandem architecture.

Effects of β-Glucan on the Release of Nitric Oxide by Macrophages Stimulated with Lipopolysaccharide

Choi, E.Y.,Lee, S.S.,Hyeon, J.Y.,Choe, S.H.,Keum, B.R.,Lim, J.M.,Park, D.C.,Choi, I.S.,Cho, K.K. Asian Australasian Association of Animal Productio 2016 Animal Bioscience Vol.29 No.11

This research analyzed the effect of ${\beta}$-glucan that is expected to alleviate the production of the inflammatory mediator in macrophagocytes, which are processed by the lipopolysaccharide (LPS) of Escherichia. The incubated layer was used for a nitric oxide (NO) analysis. The DNA-binding activation of the small unit of nuclear factor-${\kappa}B$ was measured using the enzyme-linked immunosorbent assay-based kit. In the RAW264.7 cells that were vitalized by Escherichia coli (E. coli) LPS, the ${\beta}$-glucan inhibited both the combatant and rendering phases of the inducible NO synthase (iNOS)-derived NO. ${\beta}$-Glucan increased the expression of the heme oxygenase-1 (HO-1) in the cells that were stimulated by E. coli LPS, and the HO-1 activation was inhibited by the tin protoporphyrin IX (SnPP). This shows that the NO production induced by LPS is related to the inhibition effect of ${\beta}$-glucan. The phosphorylation of c-Jun N-terminal kinases (JNK) and the p38 induced by the LPS were not influenced by the ${\beta}$-glucan, and the inhibitory ${\kappa}B-{\alpha}$ ($I{\kappa}B-{\alpha}$) decomposition was not influenced either. Instead, ${\beta}$-glucan remarkably inhibited the phosphorylation of the signal transducer and activator of transcription-1 (STAT1) that was induced by the E. coli LPS. Overall, the ${\beta}$-glucan inhibited the production of NO in macrophagocytes that was vitalized by the E. coli LPS through the HO-1 induction and the STAT1 pathways inhibition in this research. As the host immune response control by ${\beta}$-glucan weakens the progress of the inflammatory disease, ${\beta}$-glucan can be used as an effective immunomodulator.

p34 is a novel regulator of the oncogenic behavior of NEDD4-1 and PTEN

Hong, S-W,Moon, J-H,Kim, J-S,Shin, J-S,Jung, K-A,Lee, W-K,Jeong, S-Y,Hwang, J J,Lee, S-J,Suh, Y-A,Kim, I,Nam, K-Y,Han, S,Kim, J E,Kim, K-p,Hong, Y S,Lee, J-L,Lee, W-J,Choi, E K,Lee, J S,Jin, D-H,Kim, Macmillan Publishers Limited 2014 CELL DEATH AND DIFFERENTIATION Vol.21 No.1

PTEN is one of the most frequently mutated or deleted tumor suppressors in human cancers. NEDD4-1 was recently identified as the E3 ubiquitin ligase for PTEN; however, a number of important questions remain regarding the role of ubiquitination in regulating PTEN function and the mechanisms by which PTEN ubiquitination is regulated. In the present study, we demonstrated that p34, which was identified as a binding partner of NEDD4-1, controls PTEN ubiquitination by regulating NEDD4-1 protein stability. p34 interacts with the WW1 domain of NEDD4-1, an interaction that enhances NEDD4-1 stability. Expression of p34 promotes PTEN poly-ubiquitination, leading to PTEN protein degradation, whereas p34 knockdown results in PTEN mono-ubiquitination. Notably, an inverse correlation between PTEN and p34/NEDD4-1 levels was confirmed in tumor samples from colon cancer patients. Thus, p34 acts as a key regulator of the oncogenic behavior of NEDD4-1 and PTEN.

NMR study of hydrogen exchange during the B-Z transition of a DNA duplex induced by the Zα domains of yatapoxvirus E3L

Lee, E.H.,Seo, Y.J.,Ahn, H.C.,Kang, Y.M.,Kim, H.E.,Lee, Y.M.,Choi, B.S.,Lee, J.H. North-Holland Pub ; Elsevier Science Ltd 2010 FEBS letters Vol.584 No.21

The Yaba-like disease viruses (YLDV) are members of the Yatapoxvirus family and have double-stranded DNA genomes. The E3L protein, which is essential for pathogenesis in the vaccinia virus, consists of two domains: an N-terminal Z-DNA binding domain and a C-terminal RNA binding domain. The crystal structure of the E3L orthologue of YLDV (yabZα<SUB>E3L</SUB>) bound to Z-DNA revealed that the overall structure of yabZα<SUB>E3L</SUB> and its interaction with Z-DNA are very similar to those of hZα<SUB>ADAR1</SUB>. Here we have performed NMR hydrogen exchange experiments on the complexes between yabZα<SUB>E3L</SUB> and d(CGCGCG)<SUB>2</SUB> with a variety of protein-to-DNA molar ratios. This study revealed that yabZα<SUB>E3L</SUB> could efficiently change the B-form helix of the d(CGCGCG)<SUB>2</SUB> to left-handed Z-DNA via the active-mono B-Z transition pathway like hZα<SUB>ADAR1</SUB>1.

A multi-tolerant low molecular weight mannanase from Bacillus sp. CSB39 and its compatibility as an industrial biocatalyst

Regmi, S.,G.C., P.,Choi, Y.H.,Choi, Y.S.,Choi, J.E.,Cho, S.S.,Yoo, J.C. IPC Science and Technology Press ; Elsevier Scienc 2016 Enzyme and microbial technology Vol.92 No.-

<P>Bacillus sp. CSB39, isolated from popular traditional Korean food (Kimchi), produced a low molecular weight, thermostable mannanase (MnCSB39); 571.14 U/mL using locust bean gum galactomannan as a major substrate. It was purified to homogeneity using a simple and effective two-step purification strategy, Sepharose CL-6B and DEAE Sepharose Fast Flow, which resulted in 25.47% yield and 19.32-fold purity. The surfactant-, NaCl-, urea-, and protease-tolerant monomeric protein had a mass of similar to 30 kDa as analyzed by SDS-PAGE and galactomannan zymography. MnCSB39 was found to have optimal activity at pH 7.5 and temperature of 70 degrees C. The enzyme showed square 55% activity at 5.0-15% (w/v)NaCl, and square 93% of the initial activity after incubation at 37 degrees C for 60 min. Trypsin and proteinase K had no effect on MnCBS39. The enzyme showed square 80% activity in up to 3 M urea. The N-terminal amino acid sequence, ALKGDGX, did not show identity with reported mannanases, which suggests the novelty of our enzyme. Activation energy for galactomannan hydrolysis was 26.85 kjmol(-1) with a K-cat of 142.58 x 10(4) s(-1). MnCSB39 had K-m and V-max values of 0.082 mg/mL and 1099 +/- 1.0 Umg(-1), respectively. Thermodynamic parameters such as Delta H, Delta G, Delta S, Q(10), Delta G(E-S), and Delta G(E-T) supported the spontaneous formation of products and the high hydrolytic efficiency and feasibility of the enzymatic reaction, which strengthen its novelty. MnCSB39 activity was affected by metal ions, modulators, chelators, and detergents. Mannobiose was the principal end-product of hydrolysis. Bacillus subtilis CSB39 produced a maximum of 1524.44U mannanase from solid state fermentation of 1 g wheat bran. MnCSB39 was simple to purify, was active at a wide pH and temperature range, multi-stress tolerant and catalyzes a thermodynamically possible reaction, characteristics that suggests its suitability for application as an industrial biocatalyst. (C) 2016 Elsevier Inc. All rights reserved.</P>

인체와 동물에서 분리된 Coagulase Positive 포도상구균에 대한 연구

최승구 ( S G Choi ),박은병 ( E B Park ) 대한임상검사과학회 1991 대한임상검사과학회지(KJCLS) Vol.23 No.1

Coagulase positive Staphylococci was characterized in cultures from Human and Animals(dog). S. intermedius and S. hyicus subsp hyicus was distinguished from S. aureus by the following characteristics ; Coagulation of plasma at 4 h, hemolysis of blood at 24 hand manitol fermentation at 24 h. A clear separation of the coagulase positive cocci was apparent only with the acetoin reaction. Susceptibility of S. intermedius, S. hyicus subsp hyicus and S. aureus were 92%, 75%, 8%, respectively, to penicillin G. PAGE was conducted to classify for protein of S. aureus. The simirality of S. aureus used in this experiment very highed.

Inhibitory effects of Rumex japonicus Houtt. On the development of atopic dermatitis-like skin lesions in NC/Nga mice

Lee, H-S.,Kim, S-K.,Han, J-B.,Choi, H-M.,Park, J-H.,Kim, E-C.,Choi, M-S.,An, H-J.,Um, J-Y.,Kim, H-M.,Min, B-I. 경희대학교 동서의학연구소 2006 東西醫學硏究所 論文集 Vol.2006 No.-

Background Rumex japonicus Houtt. (RJH) is one of the herbs used in Eastern countries for the treatment of atopic dermatitis (AD). It has been shown to have an anti-oxidative effect in human skin disease. Objectives To examine whether RJH extract (RJH-E) suppresses the development of AD-like skin lesions in NC/Nga mice, which are induced by the repeated application of picryl chloride (PC). Methods The efficacy of RJH-E in NC/Nga mice was assessed by measuring symptom severity, scratching behaviour, Staphylococcus aureus numbers on an ear, and serum levels of IgE, interleukin (IL)-4 and interferon (IFN)-γ. Results Oral administration of RJH-E to NC/Nga mice treated with PC inhibited the development of AD-like skin lesions as exemplified by a significant decrease in total skin symptom severity scores, and a decrease in hypertrophy, hyperkeratosis and infiltration of inflammatory cells in the skin. The scratching behaviour and numbers of S. aureus, which are known to be exacerbated in AD, were also significantly reduced by RJH-E. No significant change was observed in the serum levels of IFN-γ, whereas IgE and IL-4 levels were significantly reduced by RJH-E. Conclusions These results suggest that RJH-E inhibits the development of AD-like skin lesions in NC/Nga mice by suppressing the T-helper 2 cell response. Our results indicate that RJH treatment could provide an effective alternative therapy for the management of AD.

한국인 Long Term Non Progressors로부터 분리된 HIV-1 env 및 nef 유전자와 이들의 면역 유전학적 특성 분석에 의한 질병진전 요인 규명

이주실,강춘,김성순,박근용,고운영,기미경,최병선,남정구,서순덕,이성래,구본기,박혜경,도경미,김태규,최희백,조현일,김요숙,김지영,이해정,안수진 국립보건연구원 1999 국립보건원보 Vol.36 No.-