Helicobacter pylori와 대장균의 Shuttle Vector 개발

조명제,이우곤,이상룡,김경희,안영숙,김성희,김현주,류복덕,최여정,윤영혜,백승철,전영석,이광호 경상대학교 유전공학연구소 1997 遺傳工學硏究所報 Vol.16 No.-

In this study, a vehicle vector using cryptic plasmids was constructed for gene transfer in Helicobacter pylori. pHP51(3.9 kb) and pHP489(1.2 kb) were selected for constructing vectors from cryptic plasmid of H. pylori isolates in Korea. The HindⅢ-digestedDNA fragment(1.2kb) of pHP489 and 1.6kb DNA fragment of pHP51 were ligated with a kanamycin resistance gene(aph3'-Ⅲ) from C. jejuni to produce the recombinant plasmids pHP489K and pHP51K, respectively. Transformation frequency of pHP51K by electroporation was low. But pHP489K could be effectively transformed into various H. pylori strains. In order to design an intermdiate vehicle vector for gene transfer into H. pylori, pBlueHP489K was prepared by recloning pHP489K DNA into pBluescript and pTZ19R vector. This vector permitted the DNA fragment containing pHP489 sequence, aph3'-Ⅲ, and cloned DNA to be cut and self-ligated in the SacⅠ site after cloning. ureA and ureB gene were inserted into pBlueHP489K, resulting in pBlueHP489K/AB. The DNA fragment containing pHP489, kanamycin resistance gene(aph3'-Ⅲ), and urease structural gene was cut away from pBlueHP489K/AB and self-ligated to generate pBlueHP489K/AB. pBlueHP489K/AB made urease-negative H. pylori strains restore their urease activity. By this experiment, pBlueHP489K was confirmed to be the vehicle system for transferring H. pylori genes.

Chemical Composition and Phytoestrogen Analysis of Iranian Black Pomegranate Juice Concentrate and Seeds

Choi,One-Kyun,Kim,Yong-Seong,Yu,Hye-Kyoung,Lee,Chan,Bang,Hyo-Pil,Yang,Deok-Chun,Kim,Young-Kee 한국자원식물학회 2003 Plant Resources Vol.6 No.1

In this study, as preliminary research for the development of natural estrogen supplement the chemical properties of Iranian black pomegranate juice concentrate and seeds were evaluated. Proximate compositions of pomegranate juice concentrate and seeds were as follows; crude lipid 0.4% and 8.2%, moisture 39.9% and 6.6%, crude protein 0.9% and 12.2%, ash 1.4% and 1.7%, and carbohydrate 42.0% and 84.5% respectively. Major amino acids are glutamic acid (1310.0ppm) and aspartic acid (896.2ppm) in juice concentrate, and glycine (611.1ppm) and arginin (401.6ppm) in seeds. Ascorbic acid has the highest concentration of 20.0mg/l00g in juice concentrate and 0.23mg/l00 in seeds. The compositions of unsaturated fatty acids such as linoleic acid and linolenic acid were higher than those of saturated fatty acids such as stearic palmitic acid. Major minerals were potassium, calcium and sodium, potassium was highest in both juice concentrate and seeds. Vitamins were composed of ascorbic acid (20.0mg/l00g), vitamin B$_1$ 수식 이미지(0.12mg/100g) and niacin (0.80mg/l00g) in juice concentrate, and only ascorbic acid(0.23mg/l00g) in seeds. Organic acids such as citric and L-malic acid were detected only in pomegranate juice concentrate. The contents of total polyphenols were 4.55g/L in juice concentrate and 3.5mg/l00g in seeds, respectively. Phytoestrogens detected in pomegranate juice concentrate and seeds were daidzein, quercetin, genistein and 17β-estradiol.

분광 앨립소미트리를 이용한 유리기판 위의 박막 분석

성덕경,방경윤,오영륙,최은호,안일신,김옥경 한양대학교 이학기술연구소 2003 이학기술연구지 Vol.6 No.-

Spectroscopic ellipsometry 은 다양한 물질의 광학적 특징을 연구할 수 있는 강력한 기술이다. 현재 실리콘 웨이퍼 위의 박막 분석에는 그 효율성이 확실히 입증되어 있다. 그러나 현재 산업은 유리 기판위의 박막을 분석하기를 원하고 있다. Ellipsometry로 투명기판을 분석하기 위해서는 후면 반사에의한 비간섭성 효과를 고려해야 한다. 기존의 반사공식을 투명기판의 비간섭성을 고려하여 수정하였다. 투명기판 위의 샘플과 scratch를 낸 같은 샘플을 측정 분석하여 정확성을 확인하였다. 수정된 반사공식을 이용하면 디스플레이와 같은 투명재료를 이용하는 곳에 유용하게 사용될 것으로 기대되고 있다. A Spectroscpic ellipsometry is very strong technology to study optical properties of many materials. It has already substantiated a analytic efficiency about thin films above wafers to be made of silicon. However, the current industry needs thin films analyzed on a glass. When we analyze a glass using a ellipsometry, we have to consider a efficiency of incoherence due to reflect at the back side. So then, we slightly altered the original formula. We compared a scratched sample with a sample on the glass, because off getting a exactitude. Our technical development, using the changed formula, is expected the useful utilization in a part of using gIasses such as display.

대량재해에 있어 미토콘드리아 DNA의 다형성을 이용한 개인식별

이숭덕,김기범,이윤성,최영태,신창호,이정빈 大韓法醫學會 1996 대한법의학회지 Vol.20 No.1

The mitochondrial DNA(mtDNA) is a small extranuclear DNA molecule that has been sequenced in man. the mtDNA has distinct characteristics over nuclear DNA. First, the evolutionary rate of nucleotide substitution appears to be larger compared with the nuclear DNA. Second, mtDNA is maternally inherited, so the determination of the various haplotype is unequivocal and no recombination has to be involved. Third, there is a 1.1 kb long control region, which shows severe polymorphism. These characteristics have been applied for several evolutionary study. Furthermore, they exist in cytoplasm with numerous copy number and their size, 16,569 bp is small relative to nuclear DNA, so they are more resistant to degradation or can be types in samples with only cytoplasm such as hair shaft without hair root. These presented the possibility of mtDNA as a tool in individual identification, especially when the nuclear DNA be unavailable. Actually, polymorphism in control region has been applied to individual identification for the skeletal remain found in Vietnam war. So we decided to apply the polymorphism of mtDNA in control region for the individual identification in case of mass disaster-"Downfall of Sampoong Department". Human remains from total 27 different individuals and 178 control persons were submitted for the individual identification. As the mtDNA comparison could be done through maternal lineage, 12 individual from 9 families from the control group were ruled out, because these families were consisted of father or were seeking for their father. Sequencing for the mtDNA control region was done using ABI automatic sequencer and DyeDeoxy Terminator Cycle Sequencing Kit. For convenience, sequencing reaction was done for two separate control region, region I from 16016-16401 in Anderson sequence, and regionⅡ from 048-388. First, sequences in regionⅡ of all samples and control persons were compared, and 20 samples were ruled out with no identical sequences in control group. Remaining 7 samples showed identical sequences with some of the control persons in three groups, and these identical pairs underwent mtDNA region I study. After region I study all samples except two dropped out in blood relationship. For the confirmation, STR and VNTR study such as MCT118, apoB, YNZ22, vWF, MBP, D21S11, TC-11 were done for remaining the two samples. The result denied the blood relationship between these two samples and any of the control group.

함수언어 Miranda의 Serial-Combinator Graph로의 변역

윤영우,문성헌,최관덕 嶺南大學校 工業技術硏究所 1991 연구보고 Vol.19 No.1

One of the serious problems of functional languages is the slow speed in execution of functional programs. By choosing and desiging the appropriate reduction model and machine architecture and by using clever compiling techniques this problem can be overcome. The serial-combinator-based compiling technique is known as a very efficient one for the functional languages, especially in parallel processing. The writers developed a serial-combinator-based translator and an interpretative graph reducer for Sub Miranda, a test language, on the XENIX V system. The translator is composed of three phases. The first phase translates the SubMiranda source program into λ-calculus graph, the second phase transforms the λ-calculus graph into supercombinator graph, and the final phase transforms the supercombinator graph into serial combinator graph. The correctness of the translator was examined by the intermediate codes from translating a large number of the SubMiranda programs and by the results of reducing the translated serial combinator graphs.

다량재해에 있어 미토콘드리아 DNA의 다형성을 이용한 개인식별 : 삼풍백화점 사건에 대하여

이숭덕,신창호,김기범,최영태,이윤성,이정빈 內務部 國立科學搜査硏究所 1996 國立 科學 搜査 硏究所 年報 Vol.28 No.-

열하중을 받는 복합재료 적층판의 손상에 대한 열-음향방출해석

김영복,민대홍,이덕보,최낙삼 한국비파괴검사학회 2001 한국비파괴검사학회지 Vol.21 No.3

열-음향방출(thermo-AE) 기법을 이용하여 두께 3㎜, [+456/-456]S 복합재료 적층판의 열응력 유기 손상에 대해 비파괴평가의 유효성을 연구하였다. 반복적인 열부하 사이클에 의해서 thermo-AE 사상수가 감소하는 경향이 뚜렷하게 나타나서 열부하에 따른 카이저효과가 관찰되었다. 열부하사이클중의 thermo-AE 거동을 분석하여 복합재료의 응력자유온도를 결정할 수 있었다. 초음파 C스캔, 광학현미경, 주사형 전자현미경을 통해 섬유파단과 모재파손이 관찰되었으며, 이들 파손 인자는 thermo-AE 신호의 단시간 퓨리에 변환처리에 의해 생성된 3종류의 서로 다른 시간-주파수 특성과 대응하였다. 이 특성을 이용하여 복합재료의 냉각열처리 및 반복 열부하사이클시의 손상발생과정 및 내부 마찰 거동 내역을 추적할 수 있었다.

전로제강 슬래그(BOF-Slag)를 이용한 고온탈황에 관한 연구

오광중,정덕영,최성원,조상원,손병현 釜山大學校生産技術硏究所 1996 生産技術硏究所論文集 Vol.51 No.-

고온 석탄연료가스의 탈황에 BOF-Slag의 이용가능성을 실험해 보았다. BOR-Slag의 온도, 유량, 탈황제의 특성등이 H₂S 제거효율에 미치는 영향을 조사해 본 결과, 최적의 H₂S 제거효율은 700℃에서 이루어졌고 입자크기가 작을수록 황포집능은 향상되었으나 0.214-0.631mm에서는 큰 영향이 발견되지 않았다. 조업온도 800℃이상에서는 탈황제의 응집으로 내부물질전달저항이 증가하여 탈황제의 반응성이 저하되었다. Experiments have been made to test the practical feasibility of using BOF-Slag to desulfurize hot coal-derived fuel gas. In this study, the effects of particle size, temperature, flow rate and sorbent characteristics on the H₂S removal efficiency of BOF-Slag were investigated. Experimental results showed that the removal efficiency of H₂S was optimum when the temperature was about 700℃ and the smaller sample size, the better sorbent capacity had and the sample size in the range of 0.214∼0.631mm didn't influence. When the temperature was above 800℃, the reactivity of sorbent has lowered because agglomeration of sorbent increased intraparticle transport resistance.

고온 초전도체 Y₁-x Gdx Ba₂Cu₃O7-y의 임계전류밀도에 관한 연구

전인,송승기,최덕,김영호 명지대학교자연과학연구소 1991 자연과학논문집 Vol.9 No.-

고온 초전도체 Y₁-??Nd??Ba₂Cu₃O??-??(x=00.1, 0.2, 0.3,...,1.0)를 고상 반응법을 이용하여 제작하였다. 온도에 따른 전기적 비저항의 측정과 자기적 감수율의 측정을 통하여 구한 초전도 전이온도는 92.1∼93.5K 사이에 존재하였다. 77K에서 시료의 자기적 감수율은 -5.06∼-6.83Χ10??emu/㎤ㆍOe 사이에 존재하였으며, 상온에서의 비저항은 x=0.6인 시료를 제외하고 x가 증가함에 따라 감소하였다. 수송 임계전류밀도는 자기장이 0일때 51.6∼80.6 A/㎠사이에서 측정되었으며 x가 증가함에 따라 대체로 증가하였다. 자기장을 가해준 상태에서 측정한 수송 임계전류밀도 는 자기장이 증가함에 따라 급격히 감소하였다. 자기 이력곡선으로부터 Bean의 공식을 이용하여 구한 임계전류밀도는 수송 임계전류밀도의 0.8∼2.7Χ10??배로 크게 나타났다. High-Tc superconductors Y₁-??Nd??Ba₂Cu₃O??-??(x=0.1, 0.2, 0.3,…,1.0) have been prepared by solid state reaction method. The superconduction transition temperatures determined by the temperature dependence of electric resistivity and magnetic susceptibility are in the range between 92.1 and -6.83Χ10??emu/㎤·Oe and the resistivities at room temperature are decreased as increasing Gd contents except for the value of x=0.6 sample. Transport critical current densities have the value of 51.6∼80.6 A/㎠ and are generally increased with increasing the magnetic field. Magnetic critical current densities obtained from the magnetization curve using the Bean's law are about 0.8∼2.7Χ10??times of the transport J??.

무균돈사내 급배기구의 적정위치설정에 관한 연구

권순정,손덕영,최윤호 한국의료복지시설학회 2008 의료·복지 건축 Vol.14 No.4

Construction and operation of Germ Free Pigs' facilities are very expensive because pigs' rooms and other major rooms of the facility require germfree environments. Especially, running the HVAC system of aseptic facilities requires a lot of expenses. However proper location and efficient shape of outlets/inlets for the ventilation of the room can reduce the excessive running cost. In order to do that, this study proposes alternative location and shape of ventilation outlets/inlets to the existing design pattern in germfree pigs' room. The design condition of this study is the maintenance of adequate temperature(24'C), NHs concentration level(below 1.5 ppm), and air stream speed(be1ow .25m/sec) in the pigs' room for the summer and the winter together. As the Software Program, FLUENT(Ver. 6.2) has been used for the analysis of proposed ventilation patterns, In conclusion, wall inlets and ceiling inlet/outlet are advisable in summer, wall inlets and ceiling outlets is advisable in winter. As far as the shape is concerned, diffuser type for the ceiling outlet is desirable.