Bacillus sp. FF-7에 의한 항산화물질 생산조건과 항산화 활성

차재영,김효정,전방실,박진철,옥민,조영수 한국응용생명화학회 2003 Applied Biological Chemistry (Appl Biol Chem) Vol.46 No.3

발효식품 시료로부터 분리된 세균을 DPPH(α,α¹-diphenyl-β-picrylhydrazyl) 전자공여능으로 항산화 활성을 측정하여 가장 활성이 강한 균주를 선별하여 형태학적, 생화학적, 생리학적 특성 및 16S rRNA 염기서열을 조사한 결과 Bacillus sp.으로 판명되어 FF-7로 명명하였다. DPPH 전자공여능법에 의한 Bacillus sp. FF-7이 생산하는 항산화물질의 최적 생산 배지조건은 탄소원 2% galactose와 질소원 1% tryptone 첨가였다. Bacillus sp. FF-7에 의해 생성된 항산화 물질의 활성을 DPPH 전자공여능, 흰쥐 각 조직 microsomal 실험계 및 linoleic acid 과 산화지질 실험계에서 malondialdehyde를 thiobarbituric acid(TBA)방법으로 측정하였다. 흰쥐 각 조직 microsomal 실험계에서 지질과산화에 대한 항산화 효과는 뇌(97.50%)>심장(79.95%)>신장(77.84%)>비장(77.47%)>고환(69.96%)>간장(62.45%) 순이였다. Linoleic acid의 과산화지질률 TBA법으로 측정한 결과 반응 4일째까지 억제 효과가 강하게 나타났으며, 동시에 대조구로 사용한 0.05% BHT 첨가구에서도 실험종료시까지 항산화 활성이 강하게 나타났다. The antioxidative activity of antioxidative substances produced from several bacterial strains isolated from fermented foods were tested by DPPH (α,α'-diphenyl-β-picrylhydrazyl) free radical scavenging activity. One of the strains showing the highest antioxidative activity was identified as Bacillus sp. based on the morphological, biochemical, physiological characteristics, and 165 rRNA sequence, and named FF-7. The molt optimal medium condition for the production of antioxidative substance from Bacillus sp. FF-7 was 2% galactose as carbon source and 1% tryptone as nitrogen source. The antioxidative substance produced from FF-7 in these cultural medium was also tested by in vitro experimental models, the peroxidation of linoleic acid and the peroxidation of rat tissues microsomes by using thiobarbituric acid (TBA) for assay of free rnalondialdehyde production. The antioxidative avtivity against lipid peroxidation of rat tissues microsomes was shown in the following order; brain 97.50% > heart 79.95% > kidney 77.84% > spleen 77.47% > testis 69.96% > liver 62.45%. The antioxidative substance produced from FF-7 on linoleic acid peroxidation by TBA method was effectively inhibited during four days, and 0.05% BHT(butylated hydroxytoluene) used comparative control was also effectively inhibited. Results showed that the highest antioxidative activity by DPPH method of antioxidative substance produced from Bacillus sp. FF-7 was obtained by supplementing 2% galactose as carbon source and 1% tryptone as nitrogen source in cultured medium, this substance effectively inhibited the formation of TBARS in brain microsome in vitro system and in linoleic acid peroxidation.

생쥐 배아에서 과립구 대식세포 증식인자가 착상관련 유전자 발현에 미치는 영향

김효정 ( Hyo Jung Kim ),김혜민 ( Hye Min Kim ),김재령 ( Jae Lyung Kim ),신정환 ( Jung Hwan Shin ),홍서유 ( Seo You Hong ),박은주 ( Eun Joo Park ),박원일 ( Won Il Park ),이진용 ( Jin Yong Lee ),차병헌 ( Byung Hun Cha ),문혜성 ( Hye 대한산부인과학회 2008 Obstetrics & Gynecology Science Vol.51 No.2

배경 및 목적: 과립구 대식세포 증식인자는 정상적으로 여성의 생식기관에서 분비되며 체외수정 시술시 배양액에 첨가하면 임신률을 향상시킨다는 보고가 계속되고 있다. 본 연구는 과립구 대식세포 증식인자가 생쥐의 배아에서 착상관련 유전자의 발현을 조절함으로써 임신률을 증가시킬 것이라는 가설을 입증하기 위해 시도되었다. 대상 및 방법: 200개의 생쥐 배아를 10ng/ml의 과립구 대식세포 증식인자로 처리한 군과 대조군으로 나누어 포배기 배아의 전체 유전자를 microarray 방법으로 분석하여 두 군간에 차이가 나는 유전자를 확인하였다. 이들 유전자중 착상과정에 작용할 가능성이 높은 유전자를 선별하여 real-time PCR 방법으로 mRNA 발현의 차이를 확인하였다. 결과: Microarray 결과 과립구 대식세포 증식인자에 의해 발현이 2배 이상 증가된 유전자는 64개가 확인되었고 2배 이상 감소된 유전자는 35개가 확인되었다. 증가된 유전자 중 MMP2, FABP3, Dppa5, 감소한 유전자 중 TAS1, 2배 이상 증가하지는 않았으나 integrin의 subunit 4가지와 focal adhesion kinase (FAK)는 착상에 관여할 가능성이 높다고 생각하여 real time RT-PCR을 시행하여 확인하였다. 그 결과 MMP2와 FABP3 두 가지 유전자가 real time RT-PCR에서도 발현이 증가한 것으로 확인되었다 (FABP3: 1.687배, MMP2: 1.580배). 그 이외의 유전자들을 대조군에 비해 발현이 증가하는 것으로 보이나 통계학적으로는 유의한 차이가 없었다. 결론: 과립구 대식세포 증식인자는 생쥐의 배아에서 MMP2와 FABP3의 발현을 증가시킨다. MMP2는 영양배엽세포의 침윤성을 증가시킴으로써 착상률을 증가시킬 것으로 추측된다. FABP3의 발현을 증가 역시 착상능력의 증가와 관련이 있을 것으로 추측되지만 FABP3와 착상과의 관련성에 대하여는 추가 연구가 필요할 것으로 사료된다. Objective: GM-CSF is produced in female reproductive tract and may play an important role in the process of implantation. Body of evidence suggests that GM-CSF could improve pregnancy rate in many species of mammals when it was added in culture media. The aim of this study is to assess how GM-CSF affects the expression of implantation-related genes in mouse embryo. Methods: Two hundred mouse embryos were divided into control and GM-CSF treated groups. The embryos were treated with or without 10ng/ml of GM-CSF for 72 hours. Total RNA was isolated and compared with oligo microarray. The implantation-related genes influenced by GM-CSF were repeatedly analyzed by real-time PCR. Results: After oligo microarray, 64 genes were increased and 35 genes were decreased by GM-CSF. Among those genes, MMP2, FABP3, Dppa5 and TAS1 were selected for real-time PCR analysis. Four integrins and FAK were also selected. We confirmed the increase of MMP2 and FABP3 by GM-CSF with real-time RT-PCR (1.687 and 1.580 fold, respectively). Other genes were found to be minimally increased. Conclusion: GM-CSF induces the increased expression of MMP2 mRNA in mouse embryo, and then increases the invasiveness of the trophoblast. The role of FABP3 in the process of implantation remained to be elucidated.

<잠자는 숲속의 미녀> 재안무 사례연구 - 광주시립발레단 중심으로

전효정 ( Hyo Jung Jun ),차수정 ( Su Jung Cha ) 대한무용학회 2021 대한무용학회논문집 Vol.79 No.1

This research studied the re-choreography process of “Sleeping beauty” at Gwangju City Ballet. The analysis was carried out with data collected through literature reviews, indepth interviews, participatory observations and field records. Results showed : Firstly, the play was directed in a way that it was distinctive from the original version. Secondly, the music was organized and arranged through professional consultation. Thirdly, the play attempted the fusion between ballet and Korean traditional dance. It is expected that this play would become a basic for creative ballet dance, and moreover act as a guidance for future ballet.

XPath 질의 처리를 적용한 단백질 데이터 통합 관리시스템 구축

차효성(Hyo Soung Cha),정광수(Kwang Su Jung),정영진(Young Jin Jung),류근호(Keun Ho Ryu) 한국정보과학회 2004 한국정보과학회 학술발표논문집 Vol.31 No.2Ⅱ

최근 바이오 인포매틱스 분야의 발전에 따라 방대한 양의 유전체 데이터에 대한 연구가 진행되고 있으며, 이러한 데이터를 효율적으로 다루기 위해 다양한 형태의 파일과 데이터베이스들이 사용되고 있다. 하지만 표준화의 미비로 인하여 데이터의 관리 및 변환에 어려움이 많다. 따라서 이 논문에서는 시퀀싱을 통해 생성된 유전체 및 단백질 서열 데이터의 통합 저장 관리를 위해 서열 정보의 편집, 저장 및 검색과 서열 파일 포맷 변환을 수행하는 서열 정보관리 시스템의 구현을 목적으로 한다. 이러한 요구사항을 만족사키기 위해 바이오 인포메틱스 데이터를 다루기 위한 표준으로 BSML(Bioinformatic Sequence Markup Language)을 채택하고 이질적 플랫파일들은 DTD를 기반으로 BSML 스키마로 통합 및 저장한다. 그리고 객체 관계 데이터베이스 특성을 적용하여 XML 문서를 보다 쉽게 저장 관리하고 범위 또는 구조적 질의에 효율적인 XPath 질의 처리를 위한 시스템을 개발하였다.

아동중심놀이치료 과정에서 치료자가 체험한 아동과의 관계 의미 탐색

차효정 ( Hyo Jung Cha ) 한국놀이치료학회 2010 한국놀이치료학회지(놀이치료연구) Vol.13 No.4

본 연구는 놀이치료 과정에서 치료자가 지각한 아동과의 관계 안에서의 체험(lived experience)을 심층적으로 기술함으로써 아동-치료자간의 관계의 본질을 이해하는데 그 목적이 있다. 이에 따라 ‘놀이치료자는 아동과의 관계 안에서 무엇을 체험하며 그 체험의 의미는 무엇인가?’로 연구 질문을 선정하였다. 연구 참여자들은 10년 이상의 놀이치료 경력과 한국놀이치료학회의 놀이치료사 혹은 놀이치료전문가 자격을 가진 5명으로 현재 임상에서 놀이치료를 하고 있는 사람들로 구성하였다. 연구자는 이들은 대상으로 1-3회의 면담을 실시하였고 이들과의 면담기록은 MP3와 녹음기에 녹음하여 전사기록 하였다. 이 기록은 Giorgi의 현상학적 분석절차에 따라 분석되었으며 이에 따라 의미단위들을 범주화하고 각 의미단위들을 구체적으로 기술하였다. 총 57개의 의미단위들이 파악되었고 이들은 다시 10개의 주제들로 묶여졌다. 이를 재구조화하여 최종적으로 범주화된 5개의 중심 의미들이 생성되었고 이를 통합적인 구조적 진술로 제시하였다. 연구 결과 도출된 중심 의미들은 ‘달라짐, 알아주기, 있는 그대로 받아주기, 기다려 주기, 함께 나누기’이다. 이러한 탐색을 통해 놀이치료 과정에서의 치료자-아동과의 관계 양상과 그 의미들이 드러났다. 이를 통해 놀이치료 및 치료자 역할 그리고 아동-치료자간의 관계가 다른 일상의 관계와 차별되는 측면을 이해함으로써 치료적인 관계의 본질적인 측면들을 이해할 수 있다는 점에서 연구의 의의가 있다. The purpose of this study was to present an in-depth description of practical experience about therapist`s perception on relationship between a child and a therapist. This study consists of two questions about the therapist`s personal experiences and meanings of their experiences. All of five participants in this research are certified by the Korean Association for Play Therapy and activity working as play therapists. Data were collected by interview from all participants and recorded their reports. The data were analyzed using the procedure of Giorgi(1985)`s phenomenological psychological method. According th the analysis, the data were classified into 57 ``meaning units`` and investigated 10 themes. Finally, the data were further by 5 ``Focal meaning``. The focal meanings were ``transition``, ``awareness``, ``acceptance``, ``waiting``, ``sharing``. These results helped to understand essential factors in therapeutic relationship between a child and a therapist.

원예치료가 지적장애청년들의 자기표현능력 향상에 미치는 효과

손경옥(Kyoung Ok Son), 차쾌자(Koae Ja Cha), 김효정(Hyo Jung Kim), 김경희(Kyung Hee Kim), 양희승(Hee Seung Yang) 인간식물환경학회 2023 인간식물환경학회 학술대회 Vol.2023 No.1

Polymorphisms in the Caspase Genes and the Risk of Lung Cancer

Lee, Shin Yup,Choi, Yi Young,Choi, Jin Eun,Kim, Min Jung,Kim, Jong-Sik,Jung, Deuk Kju,Kang, Hyo-Gyoung,Jeon, Hyo-Sung,Lee, Won Kee,Jin, Guang,Cha, Seung Ick,Kim, Chang Ho,Jung, Tae Hoon,Park, Jae Yong Elsevier 2010 JOURNAL OF THORACIC ONCOLOGY Vol.5 No.8

<P>INTRODUCTION:: Caspases (CASPs) are important regulators and executioners in apoptosis pathway and play a crucial role in the development and progression of cancer. On the basis of the interactions of CASPs in the apoptotic pathway, we evaluated the association between 11 polymorphisms of CASP3, 6, 7, 8, 9, and 10 genes and the risk of lung cancer. METHODS:: The genotypes were determined in 720 patients with lung cancer and 720 healthy controls frequency matched for age and gender. RESULTS:: In individual polymorphism analysis, the CASP7 rs2227310C>G and CASP9 rs4645981C>T were associated with 1.40-fold and 1.28-fold increased risk of lung cancer under recessive and dominant models for the variant allele of each polymorphism, respectively. In the case of the CASP3 77G>A, subjects with the GG genotype were at a 1.19-fold increased risk of lung cancer compared with those with at least one variant allele. When the three polymorphisms were combined, the risk of lung cancer increased in a dose-dependent manner as the number of risk genotypes increased (ptrend <0.001). Subjects with two and three risk genotypes carried a significantly increased risk of lung cancer compared with those with zero risk genotype (adjusted odds ratio = 1.56, 95% confidence interval = 1.14-2.13, p = 0.005 and adjusted odds ratio = 2.54, 95% confidence interval = 1.28-5.02, p = 0.008, respectively). CONCLUSIONS:: These results suggest that a combined analysis of these three CASP gene polymorphisms might better predict the risk of lung cancer than analysis of a single polymorphism.</P>

Nonmonotonic Effects of Chronic Low-Dose Di(2-ethylhexyl) Phthalate on Gonadal Weight and Reproduction

Cha, Sunyeong,Jung, Kayeon,Lee, Min Young,Hwang, Yeon Jeong,Yang, Eunhyeok,Lee, Sung-Ho,Jung, Hyo-il,Cheon, Yong-Pil The Korean Society of Developmental Biology 2018 발생과 생식 Vol.22 No.1

Endocrine disruptors have been concerned in toxicology but now challenged as physiological point especially concerned with exposing dose and period. In this study the low-dose chronic administration of di(2-ethylhexyl) phthaltae (DEHP) during reproductive period was examined to evaluate the possible roles. Adult male and female CD-1 mice were exposed to DEHP with drinking water containing $133{\mu}g/L$ and $1,330{\mu}g/L$ DEHP in water according to OECD 433 guide line and sacrificed just after weaning. The weights of uterus and ovary were decreased by drinking of $1,330{\mu}g/L$ DEHP water. There was not adverse effects on either accumulated mating rate and mating rate depend on estrus stage, pregnancy duration, and sex ration at birth. However, the accumulated rate of successful delivery and litter size were significantly high at $1,330{\mu}g/L$ DEHP water. The number of epididymal sperm was significantly increased by drinking of $1,330{\mu}g/L$ DEHP water. In addition, the number of follicles (primary, secondary, tertiary) were more many than control at $1,330{\mu}g/L$ DEHP water drunk mother. Though further studies are needed to identify what are the mechanism of DEHP in folliculogenesis and spermatogenesis. From this study we firstly report the effect of low-dose chronic administration of DEHP with drinking could change the ovarian follicle population size and spermatogenesis rate. Put together, those finding is different from previous high-dose effects and suggest the physiological role of DEHP in gonads and uterus.

Disturbing Effects of Chronic Low-dose 4-Nonylphenol exposing on Gonadal Weight and Reproductive Outcome over One-generation

Cha, Sunyeong,Baek, Jeong Won,Ji, Hye Jin,Choi, Jun Hee,Kim, Chaelim,Lee, Min Young,Hwang, Yeon Jeong,Yang, Eunhyeok,Lee, Sung-Ho,Jung, Hyo-il,Cheon, Yong-Pil The Korean Society of Developmental Biology 2017 발생과 생식 Vol.21 No.2

4-Nonylphenol (NP) is a surfactant that is a well-known and widespread estrogenic endocrine disrupting chemical (EDC). Although it has been known that the affinity of NP to ERs is low, it has been suggested that low-dose NP has toxicity. In the present study, the endocrine disrupting effects on reproduction, and the weight of gonads, epididymis, and uterus were evaluated with the chronic lower-dose NP exposing. This study was designed by following the OECD test guideline 443 and subjected to a complete necropsy. In male, NP had an effect on the weight of the testis and epididymis in both $F_0$ and $F_1$. In females, NP decreased the weight of ovary and uterus in $F_0$ but not in pre-pubertal $F_1$ pubs. Fertility of male and female in $F_0$ or $F_1$ was no related with NP administration. The number of caudal-epididymal sperm by body weight (BW) was not different between groups in both $F_0$ and $F_1$. Besides, the difference of the sperm number between generations was not detected. The number of ovulated oocytes was similar between groups in $F_0$, but significantly decreased in NP 50 group of $F_1$. The litter size and sex ratios of offspring in $F_1$ and $F_2$ were not different. The accumulated mating rate and gestation period were not affected by the NP administration. Those results shows that chronic lower-dose NP administration has an effect of endocrine disruptor on the weight of gonads and epididymis of $F_0$ and $F_1$ but not in reproduction. Based on the results, it is suggested that chronic lower-dose NP exposing causes endocrine disruption in the weight of gonad and epididymis but not in the reproductive ability of next generations.

A Study on the properties of aluminum nitride films on the Al7075 deposited by pulsed DC reactive magnetron sputtering

Jung-hyo Kim,Byung-Chul Cha,Keun-Hak Lee,Won-Wook Park 한국표면공학회 2012 한국표면공학회 학술발표회 초록집 Vol.2012 No.11