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순무(Brassica campestris ssp. rapa) 뿌리로부터 지질화합물의 분리 및 동정
방면호,오영준,유종수,한민우,정해곤,정태숙,최명숙,이경태,김세영,백남인 경희대학교식량자원개발연구소 2007 硏究論文集 Vol.26 No.1
순무 뿌리로부터 활성 물질을 분리 동정 하기 위하여 80% MeOH 수용액으로 추출하고 이를 여과, 감압 농축하여 MeOH추출물을 얻었다. 이를 EtOAc분획, n-BuOH분획, H_(2)O분획으로 나누었으며, EtOAc분획에 대해 silica gel column chromatography를 실시하여 3종의 지질화합물을 분리 정제하였다. ^(1)H-NMR, ^(13)C-NMR, DEPT spectrum 및 Mass spectrum등을 통하여 palmitic acid methyl ester(compound 1), linolenic acid methyl ester(compound 2), linoleic acid methyl ester(compound 3)으로 구조를 결정하였다. In order to isolate and identify the active compound from the Brassica campestris ssp rapa, the roots and the root skin were extracted with 80% aqueous MeOH, and the concentrated extract was partitioned with EtOAc, n-BuOH and H_(2)O. From the EtOAc fraction, three compounds were isolated through the repeated silica gel column chromatographies. From the result of spectral data including NMR and MS, the chemical structures of the compound were determined as palmitic acid methyl ester (compound 1), linolenic acid methyl ester (compound 2), linoleic acid methyl ester (compound 3).
싸주아리쑥(Artemisia herba)으로부터 phytol의 분리
유종수,방면호,정해곤,정태숙,최명숙,이경태,백남인 경희대학교식량자원개발연구소 2006 硏究論文集 Vol.25 No.-
싸주아리쑥 지상부의 80% MeOH 추출물로부터 얻어진 EtOAc 분획물에 대하여 silica gel 및 ODS column chromatography를 반복하여 diterpene을 분리하였다. 화합물의 화학구조는, NMR, MS 및 IR 등의 스펙트럼을 해석하여 phytol로 동정하였다. The aerial part of Artemisia herba was extracted with 80% aqueous MeOH and the concentrated extract was partitioned with EtOAc, n-BuOH and H_(2)O, successively. From the EtOAc fraction, a terpenoid was isolated through the repeated silica gel and ODS column chromatographies. From the result of physico-chemical data including NMR, MS and IR, the chemical structure of the compound was determined as phytol.
유종수,방면호,정해곤,정태숙,최명숙,이경태,김세영,백남인 경희대학교식량자원개발연구소 2007 硏究論文集 Vol.26 No.1
강화 특화작목의 건강기능성 식품 제품화의 가능성을 타진하고 재배작물의 표준화 연구를 위해 사자발쑥, 싸주아리쑥 그리고 순무에 대해 총 폴리페놀과 총 플라보노이드 함량을 측정하였다. 총 폴리페놀 함량은 사자발쑥이 9.5mg/g, 싸주아리쑥이 7.4mg/g, 순무가 1.8mg/g으로 총 플라보노이드 함량은 사자발쑥이 4.3mg/g, 싸주아리쑥이 3.6mg/g, 순무가 0.4mg/g으로 확인 되었다. 또한 강화 약쑥의 유효지표성분인 eupatilin과 jaceosidin이 사자발쑥에는 각각 208mg/100g, 118mg/100g, 싸주아리쑥에는 199mg/100g, 111mg/100g 함유된 것을 HPLC를 통해 확인하였다. This study was conducted to investigate the contents of total polyphenol and total flavonoid of three Ganghwa indigenous crops, two varieties of Artemisia princeps Pampanini (Sajabalssuk, Sajuarissuk) and Brassica campestris ssp rapa. Total polyphenol and total flavonoid contents of Sajabalssuk were 9.5 and 4.3 mg/g, those of Sajuarissuk were 7.4 and 3.6 mg/g, those of B. campestris ssp rapa were 1.8 and 0.4 mg/g, respectively. The contents of eupatilin and jaceosidin in A. princeps Pampanini which were calculated by HPLC, were determined 208 mg/100g, 118 mg/100g in Sajabalssuk and 199 mg/100g, 111 mg/100g in Sajuarissuk, respectively.
Ji-Hae Park,Do-Gyeong Lee,Seung-Woo Yeon,Hyuk-Sang Kwon,Jong-Hee Ko,Dong-Jin Shin,Han-Sol Park,Yong-Soon Kim,Myun-Ho Bang,NAM-IN BAEK 대한약학회 2011 Archives of Pharmacal Research Vol.34 No.4
The silkworm (Bombyx mori L.) droppings were extracted with 80% aqueous MeOH, and the concentrated extract was partitioned in succession with EtOAc, n-BuOH, and H_2O. From the EtOAc fraction, five megastigmane sesquiterpenes were isolated through repeated silica gel and ODS column chromatography. According to the results of spectroscopic data, such as NMR, MS, and IR, the chemical structures of the isolated compounds were determined as (3S,5R,8R)-3,5-dihydroxymegastigma-6,7-dien-9-one (1), (S)-dehydrovomifoliol (2), (6R,7E,9R)-9-hydroxy-4,7-megastigmadien-3-one (3), (3S,5R,6S,7E)-3,5,6-trihydroxy-7-megastigmen-9-one (4), (6R,9R)-9-hydroxy-4-megastigmen-3-one (5). Compounds 2 through 5 were isolated for the first time from silkworm droppings. GC/MS analysis indicated silkworm powder contained compound 3, and mulberry leaves contained compound 4. Compounds 1 and 5 increased the expression of heme oxygenase-1 and SIRT1 in HepG2 and HEK239 cells, respectively. Heme oxygenase-1 is considered to be an antioxidant enzyme that catabolizes heme to carbon monoxide, free iron and biliverdin, while SIRT1 is the mammalian homologue of the yeast silent information regulator (Sir)-2, which are involved in the suppression of inflammatory mediators or factors that may be used to improve atopy-related symptoms.
Inhibitory Effects of Actinidiamide from <i>Actinidia polygama</i> on Allergy and Inflammation
BANG, Myun-Ho,CHAE, In-Gyeong,LEE, Eun-Ju,BAEK, Nam-In,BAEK, Yoon-Su,LEE, Dae-Young,LEE, In-Seon,LEE, Sam-Pin,YANG, Seun-Ah Japan Society for Bioscience, Biotechnology, and A 2012 Bioscience, Biotechnology, and Biochemistry Vol.76 No.2
<P><I>Actinidia polygama</I> Max. was subjected to supercritical fluid extraction (SFE), and the resulting ethanol extract of marc (SFEM) was subjected to sequential fractionation with various solvents. Each extract and fraction was assayed for anti-inflammatory effect. The ethyl acetate fraction (EtOAc) contained the highest level (70.8% inhibition) of anti-inflammatory activity. In order to identify the active constituents, the EtOAc fraction was further fractionated by silica gel and ODS column chromatography. By activity-guided fractionation, an active ceramide was identified as the anti-inflammatory component, and its structure was determined by NMR and MS analysis. The novel ceramide was named actinidiamide, and was found significantly to inhibit nitric oxide (NO) production (30.6% inhibition at 1 μg/mL) in lipopolysaccaride (LPS)-stimulated RAW 264.7 cells and β-hexosaminidase release (91.8% inhibition at 1 μg/mL) in IgE-sensitized RBL-2H3 cells. Thus the presence of actinidiamide conveys allergy and inflammation treatment ability to <I>A. polygama</I>.</P>
Phytol, SSADH Inhibitory Diterpenoid of Lactuca sativa
Bang, Myun-Ho,Choi, Soo-Young,Jang, Tae-O,Kim, Sang-Kook,Kwon, Oh-Shin,Kang, Tae-Cheon,Won, Moo-Ho,Park, Jin-Seu,Baek, Nam-In The Pharmaceutical Society of Korea 2002 Archives of Pharmacal Research Vol.25 No.5
The succinic semialdehyde dehydrogenase (SSADH) inhibitory component was isolated from the EtOAc fraction of Lactuca sativa through repeated column chromatography; then, it was identified as phytol, a diterpenoid, based on the interpretation of several spectral data. Incubation of SSADH with the phytol results in a time-dependent loss of enzymatic activity, suggesting that enzyme modification is irreversible. The inactivation followed pseudo-first-order kinetics with the second-rate order constant of $6.15{\times}10^{-2}mM^{-1}min^{-1}.$ Complete protection from inactivation was afforded by the coenzyme $NAD^{+}$, whereas substrate succinic semialdehyde failed to prevent the inactivation of the enzyme; therefore, it seems likely that phytol covalently binds at or near the active site of the enzyme. It is postulated that the phytol is able to elevate the neurotransmitter GABA levels in central nervous system through its inhibitory action on one of the GABA degradative enzymes, SSADH.
A new miroestrol glycoside from the roots of Pueraria mirifica
Bang, Myun-Ho,Lee, Do-Gyeong,Baek, Yoon-Su,Cho, Jin-Gyeong,Han, Min-woo,Choi, Kyoung-Sook,Chung, Dae-Kyun,Ko, Sung-kwon,Oh, Chang-Hwan,Cho, Soo-Yeul,Chai, Kap-Yong,Kim, Jin-Ho,Baek, Nam-In Springer-Verlag 2013 Chemistry of natural compounds Vol.49 No.3
13-Hydroxy-9Z,11E,15E-octadecatrienoic Acid from the Leaves of Cucurbita moschata
Bang, Myun-Ho,Han, Jae-Taek,Kim, Hae-Yeong,Park, Young-Doo,Park, Chang-Ho,Lee, Kang-Ro,Baek, Nam-In The Pharmaceutical Society of Korea 2002 Archives of Pharmacal Research Vol.25 No.4
A new unsaturated hydroxy fatty acid was isolated from the leaves of Cucurbita moschata through repeated silica gel column chromatography and chemical methods. The structure of the new fatty acid was determined as 13-hydroxy-9, 11, 15-octadecatrienoic acid on the basis of several spectral data including 2D-NMR. The stererostructures of double bonds were determined to be 9Z, 11 E and 15E by coupling patterns of related proton signals in the $^1H-NMR$ and NOESY experiments.