VDT작업별 정신사회적 스트레스와 근골격계 장애에 관한 연구

백남종,강종두,주영수,배인근,권호장,박종만,조수헌,김돈규,김재용,최홍렬 大韓産業醫學會 1998 대한직업환경의학회지 Vol.10 No.4

It has been hypothesized that jobs that have both high psychological demands and low decision latitude("job strain") can lead to musculoskeletal disorder. The objective of this study was to test whether job strain was correlated with the presence of work-related musculoskeletal disorder, especially myofascial pain syndrome(MPS). Information on demographic factors, confounders such as household load and taking care of children or not, and scores for decision latitude, job demand, and social support was obtained by self-administered questionnaire, which had been developed in Korean language, by adopting NIOSH instrument and Extended Karasek Model(16 items). All subjects were also examined by rehabilitation medicine specialists for musculoskeletal disorders. Subjects(n=370) could be categorized into 4 groups, these were, housewives(n=89), shipyard CAD workers(n=89), general female workers(n=79; nurses, insurance counselors, public officials, clerks, etc), and telephone directory assistance operators(n=113). Results from univariate analyses indicated that all demographic factors, all confounders, scores for decision latitude and social support were not associated with the risk of musculoskeletal disorder. However, score for job demand was higher in musculoskeletal disorder cases than others. In subgroup analysis, this association was convinced again, in telephone directory assistance operators. Job strain model showed that the group of telephone directory assistance operators was high-stain group, and OR of musculo skeletal disorder was 2.446(95% C. I. : 1.174, 5.096), when comparing this with the low strain group. In conclusion, job strain is a risk factor for work-related musculoskeletal disorder.

식중독균 항원(Salmonella typhimurium)에 의하여 생성된 계란항체(IgY) 특성과 항균 효과

백반석,한준표,배만종 동아시아식생활학회 1999 동아시아식생활학회지 Vol.9 No.2

This study was carried out to get a industrial information about a possibility of IgY antibody production, antimicrobial activity and properties of IgY antibody in egg yolk. The residual antibody activities of IgY were 91.5% after heating for 30min at 60℃. At the same time, these activities, were 73.2% after heating 15min at 65℃ and decreased vapidly at 70℃ and little antibody activity was left after heating for 15min at 80℃. When the prepared IgY was incubated at various pH ranges from 7 to 2 for 5hr at 37℃, the antibody activity was stable from pH 7 to 4 and remained to 69.8% at pH 3.

EFG와 IGF-I의 첨가배양이 돼지 미성숙 난포란의 체외성숙과 배발달에 미치는 영향

백준종,한만희,박병권,서길응,이규승 충남대학교 농업과학연구소 2007 농업과학연구 Vol.34 No.1

The present study was carried out to examine the effect of EGF and IGF-I in vitro maturation (IVM) of porcine oocytes and development of porcine IVM/IVF embryos. The results were summarized as follows : 1. The rates of nuclear maturation, penetrated oocytes, pronuclear formation, polyspermic oocytes and mean numbers of the penetrated sperm were not different in NCSU-23 maturation medium with 0, 1. 5 and 10 ng/ml EGF and IGF-I (P>0.05). 2. The rates of blastocyst formation at day 7 after in vitro fertilization in 0, 1, 5 and 10 ng/ml EGF groups were 11.2±1.5%, 15.0±8.3%, 16.8±2.8% and 21.4±2.0%, also 0, 1, 5 and 10 ng/ml IGF- I groups were 11.2±1.5%, 15.0±8.3%. 16.8±2.8% and 21.4±2.0%, respectively. In the total cells case, EGF groups were 22.8±3.7, 25.7±5.5, 26.0±4.2 and 35.1±4.7, also IGF-I groups were 21.5±3.7, 25.2±2.8, 26.2±2.9 and 33.2±3.6, respectively. Both 10 ng/ml EGF group and 10 ng/ml IGF-I group were significantly higher than those of other treatment groups (P<0.05). 3. The rates of blastocyst formation at day 7 in the NCSU23 culture medium of porcine IVF-produced embryos with 0, 1, 5, and 10 ng/ml EGF groups were 14.0±1.7%, 16.2±1.4%, 16.9±1.2% and 23.1±1.6%, also 0, 1, 5, 10 ng/ml IGF-I groups were 13.6±1.7, 15.7±4.5. 16.0±0.2 and 25.0±0.8, respectively. And in the total cells case, EGF grups were 21.8±2.9, 25.2+2.8, 39.7±2.7 and 46.2±3.6, also IGF-I groups were 20.7±2.9, 26.2±2.9, 24.6±2.4 and 46.1±3.5, respectively. Both 10 ng/ml EGF group and 10 ng/ml IGF-I group were significantly higher than those of any other treatment groups (P<0.05). In conclusion, these results suggested that the addition of 10 ng/ml EGF and IGF-I were effective on the blastocyst formation and total cells of blastocysts.

식중독균 항원(Salmonella typhimurium)에 의한 계란항체(IgY) 생산성과 항체특성 및 항균효과

백경연,손규목,배만종,백반석,한준표 경산대학교 생명자원개발연구소 1999 생명자원과 산업 Vol.3 No.-

본 연구는 식중독균을 항원으로 하여 산란계에 면역시킨 후 계란 항체(IgY)의 생산 가능성을 검토하고, 그 항체의 특성과 항균성을 조사하고자 실시하였다. 그 방법으로는 항원으로 사용된 Salmonella typhimurium(ATCC 14028) 등을 incomplete Freund's adjuvant와 동량으로 혼합하여 면역시킨 후 10주간 생산된 계란을 수집하여, 계란항체(IgY)의 분리 및 식중독균 항원에 대한 항체의 특성, 항균성, 생화학적 특성을 측정하였다. S. typhimurium 항원에 면역된 산란계의 계란항체(IgY)생산 및 증가를 측정한 결과, 산란계가 이들 항원에 면역반응을 일으켜 2주 후 혈청과 난황에서 각각 normal군에 비하여 뚜렷한 항체가 생성능을 보였으며, 혈청내의 IgG생성에 비하여 계란항체(IgY)의 생성이 다소 낮은 것으로 나타났다. 혈청 및 난황내의 단백질 함량은 normol군에 비하여 약간 높은 경향을 나타내어 산란계의 혈청 및 난황내의 단백질이 이들 항원에 의하여 증가하는 것으로 나타났다. DEAE-Sephacel column을 통과한 fraction은 두 개의 peak를 나타내었다. 각 fraction의 IgY농도를 ELISA법으로 확인한 결과 두 번째 peak에서 대부분 측정되었다. 분리 정제된 IgY의 분자량을 SDS-polyacrylamide 전기영동법으로 측정한 결과, heavy chain 72∼75KD, light chain 30∼40KD정도였다. Anti-S. typhimurium 항체의 온도에 대한 활성은 60℃에서 30분 가열시 91.5%, 65℃에서 15분 가열시 각각 73.2%의 활성도를 나타내었으며, 80℃에서 15분간 가열시는 거의 활성이 없는 것으로 나타났다. pH에 대한 안정성은 pH 7부터 pH 4까지는각각 98.7%의 활성도를 유지하다가 pH 3부터 급격히 감소하여, pH 2에서는 9.6%로 거의 활성도를 나타내지 않았다. 항균활성을 응집가, 생균수, disc diffusion susceptibility test 등으로 측정한 결과 anti-S. typhimurium 항체의 항균활성도는 매우 미약하게 나타났다. 이상의 결과에서, 본 실험에서 사용된 IgY는 항원에 대한 특이성 및 생화학적 특성 등을 고려해 볼 때 앞으로 식중독균 제거 및 예방 치료뿐만 아니라 식품산업 소재, 각종 질병예방을 위한 의약품 소재, 연구용 kit와 진단용 kit 및 축산분야 등 여러 분야에 응용될 수 있으리라 기대된다. This study was carried out to investigated to get a industrial information from discussion about productive a possibility if IgY antibody, antimicrobial activity and properties of IgY antibody in egg yolk. Immunoglobulin Y in egg yolk and immunoglobulin G in serum were obtained from gens which were immunized with Salmonella typhimurium(ATCC14028). These antibody titers were evaluated in serum and egg yolk by ELISA. After the initial immunization the anti-Salmonella typhimurium IgY antibody level gradually were decreased from firth week to tenth week. On the other hand, the antibody level in the serum were increased from the first week, reaching its peak in the sixth week. The residual antibody activities of IgY were 91.5% after heating for 30min at 60℃. At the same time, were 73.2% after heating 15min at 65℃ and decreased vapidly at 70℃ and little antibody activity was left after heating for 15min at 80℃. When the prepared IgY was stable from pH 7 to 4 and remained to 69.8% at pH 3, respectively.

식중독균 항원(Escherichia coli)에 의한 계란항체(IgY)생산성과 항체특성

백반석,배만종 경산대학교 생명자원개발연구소 2000 생명자원과 산업 Vol.4 No.-

식중독 균인 Escherichia coli를 항원으로 하여 산란계에 면역시켜 계란 항체(IgY)의 생산성과 항체의 특성을 조사하고자 실시하였다. 최초 면역후 형청내의 IgG는 1주부터 생성되기 시작하였으나, 난황내의 IgY는 2주부터 생성되기 시작하였다. 이것은 모체인 산란계에서 먼저 항체가 생성된 후 계란의 난황에 이행되어 농축되는 것으로 IgY의 생성과정을 확인하는 결과였다. 또한 혈청 및 난황내의 단백질 함량도 일반 대조군에 비해 다소 높은 경향을 보여 면역단백질의 증가를 뒷받침하였다. 본 실험에서 생산된 IgY는 65℃까지는 다소 안정된 경향을 나타내었으나, 70℃에서는 활성이 급격히 떨어지는 것으로 나타났다. pH에 대한 안정성은 비교적 안정하여 pH4에서도 90% 이상의 활성을 유지하였다. 이러한 결과로 볼 때 계란을 이용한 항체의 생산은 대량생산이 가능할 뿐만 아니라, 항원에 대한 특이성을 이용한 진단 kit제조, 의약품등 다양한 분야에 응용될 수 있으리라 기대된다. This study was investigated to make industrial base from discussion about production possible of IgY antibody, properties of IgY antibody in egg yolk. Immunoglobulin Y in egg yolk and immungoglobulin G in serum were obtained from hens which were immunized with Escherichia coli(ATCC 11775). The antibody titer were evaluate in serum and egg yolk by ELISA. After the initial immunization, the anti-Escherichia coli IgY antibody level in egg yolk increased from the two week, reaching its peak in the fifth week. On the other hand, the antibody level in the serum began to increase from the first week, reaching its peak in the eighth week. The residual antibody activities of IgY after heating at 60℃ for 30min and at 65℃ for 15min were 92%, 70% respectively, and decreased sharply at 70℃ and little antibody activity was left after heating at 80℃ for 15min. When the IgY preparation was incubated at various pHs ranging from 7 to 2 at 37℃ for 5hr. With result that the antibody acivity was stable from pH 5 to 4 and remained to 99% and 90% respectively.

초등학교 체육교육과정운영의 준비성에 관한 연구

윤만형,백종수 한국스포츠리서치 2003 한국 스포츠 리서치 Vol.14 No.3

The purpose of this study was to investigate 'the readiness for curriculum management'. The population of this study was all 3-6 grade classroom teachers and physical education specialists of 115 elementary schools in Kwang-ju city. In considering the results of this study totally, the problems of elementary school physical education were summarized as follows that physical education curriculum management were not executed in normally, physical education teaching was not efficient in respect of teaching quality, and physical education teaching environment was very poor so that it might impede the activation of physical education. In order to solve these causes and present methods for improvement of physical education, teacher training, institutional improvement, financial investment are considered. To begin with, in respect of teacher training, teacher education programs should be developed to enhance the expertism on physical education and establish value perception on it. Next, in respect of institutional improvement, for teacher to have concern to physical education, to enhance the efforts of teachers, and to be source of supervisor teaching the school teacher and student teacher(preparing teacher), the institutional mechanism should be made and physical education specialist system should be improved. Last, in respect of financial investment, a resolute investment should be made to improve the physical education environment.

식중독균 항원(Salmonella typhimurium)에 의한 계란항체(IgY) 생산성과 분리 정제

한준표,백반석,배만종 동아시아식생활학회 1999 동아시아식생활학회지 Vol.9 No.2

This study was carried out to get a industrial information about a possibility of IgY antibody production, antimicrobial activity and properties of IgY antibody in egg yolk. After the initial immunization the anti-Salmonella typhimurium IgY antibody level gradually were decreased from firth week to tenth week. On the other hand, the antibody level in the serum were increased from the first week, reaching its peak in the sixth week. Molecular weights of IgY were estimated approximately 72~75KD in a heavy chain and 30~40KD in a light chain by electrophoresis.

감초 추출물이 면역응답에 미치는 영향

심호기,백반석,황성원,배만종 경산대학교 생명자원개발연구소 1996 생명자원과 산업 Vol.1 No.-

감초에서 분리한 각각의 추출물(HWE, ME, AE)을 BALB/c mouse에 투여하여 탐식작용, 용혈반형성, 용혈소 역가특정 및 Rosette형성 실험을 통해 감초가 면역응답에 미치는 영향을 조사하였다. 1. 탐식능 측정에서 복강침출세포(PEC)와 비장세포(spleen cell)는 CON군에 비하여 가각의 약물투여군이 Candida parapsilosis에 대한 효과가 약간 높은 경향을 나타냈지만 유의성은 없었다. 2. 용혈반 형성 및 용혈소 역가측정에서는 CON군에 비하여 HWE, ME, AE투여 군이 유의성을 나타냈다. 3. Rosette형성에 대한 측정에서는 CON군에 비하여 ME, AE투여 군이 높은 유의성을 나타냈다. 본 실험 결과에서 감초 추출물들은 Candida paraprapsilosis에 대하여 약간의 탐식증진효과를 나타냈으며, 항체생성세포의 용혈반 시험 및 용혈소 역가측정과 Rosette형성측정에서의 유의성을 나타낸 것은 감초가 세포성 면역을 주도하는 T세포를 활성화시킬 뿐만 아니라, 체액성 면역을 주도하는 B세포를 활성화시킨다는 것을 확인 할 수 있다. The inverstigation of effecting immune response on BALB/c mouse was experimented with hot water extract(HWE), 50% methanol extract(ME) and aaceton extract(AE) 〔which was extracted from Glycyrrhiza glabra〕. The experiment was carried out by phagocytosis, hemolytic plaque assay, hemolysin titration and rosette assay. Peritoneal exudative cells and spleen cells in phagocytosis ability; the experimental group has shown a slight increase in Candida parapsilosis(HWE, ME & AE). The experiment shows no relevant correlation. The experiment of hemolytic plaque assay and hemolysin titration was treated by HWE, ME and AE. The experimental group was treated by ME and AE through the measurement of rosette assay. The experimental group has given significant increase compared with the control. As the result, the extracts of Glycyrrhiza glabra (HWE, ME and AE) has effedted slightly to Cnadida parapsilosis in phagocytosis. The reason of effecting the immunocyte in hemolysin titration, rosette assay and hemolytic plaque assay ; Glycyrrhiza glabra has activated the T-cell which controlled cell mediated immunity. Same with B-cell which could also control humoral immunity.

풍선 확장술로 치료한 담관결석과 동반된 총담관 막양구조

백성민 ( Sung Min Baek ),김동욱 ( Dong Uk Kim ),장선미 ( Sun Mi Jang ),송병구 ( Byeong Gu Song ),박종만 ( Jong Man Park ),이대성 ( Dae Sung Lee ),김중근 ( Joong Keun Kim ),황경림 ( Kyung Lim Hwang ) 대한췌장담도학회 2014 대한췌담도학회지 Vol.19 No.3

Bile duct web is very rare disease and it`s etiology is controversial. Some webs are occurred in the presence of chronic inflammation, frequently associated with bile duct stone, but others are thought to be congenital. Many patients with bile duct web are asymptomatic, but they sometimes present symptom of biliary obstruction and cholangitis. It can be diagnosed by endoscopic retrograde cholangiopancreatogram, typically appearing as thin and shelf like radiolucent ring. We report a case of the common bile duct web with bile duct stones diagnosed by Endoscopic retrograde cholangiopancreatography (ERCP) in a 65-year-old man. The patient was treated by balloon dilatation successfully.

제주 차귀도 연안해역 미생물 분포 및 이화학적 특성

문영건,김만철,이준백,송춘복,여인규,김종만,박근태,손홍주,최우봉,허문수 한국환경과학회 2006 한국환경과학회지 Vol.15 No.3