감초 추출물이 면역 응답에 미치는 영향

심호기,박무희,최청,배만종 한국식품영양학회 1997 韓國食品營養學會誌 Vol.10 No.4

감초에서 분리한 각각의 추출물(GHE, GME, GAE)을 BALB/c mice에 투여하여 탐식작용, 용혈 반형성, 용혈소 역가 측정 및 rosette 형성 실험을 통해 감초가 면역응답에 미치는 영향을 조사하였다. 1. 탐식능 측정에서 복강침출세포(PEC)와 비장세포(spleen cells)는 CON군에 비하여 각각의 약물투여군이 Candida parapsilosis에 대한 효과가 다소 높은 경향을 나타내었지만, 유의성은 없었다. 2. 용혈반 형성 및 용혈소 역가측정에서는 CON군에 비하여 GHE, GME 투여군 및 GAE 투여 군이 높게 나타났다. 3. Rosette형성에 대한 측정에서는 CON군에 비하여 GME, GAE 투여군이 높은 유의성을 나타내었다. This study was conduced to investigate on immune response of the hot water extract(PHE), 50% methanol extract(PME) and acetone extract(PAE) from Glycyrrhiza glabra. The experiment was carried out by phagocytosis, plaque forming cell(PEC), hemolysin titration(HY) and rosette forming cell(RFC) assay by using BALB/c mice. The results obtained from this study are as follow ; The effects of Glycyrrhiza glabra extracts on phygocytosis was tended to beslight increase in GME and GAE groups compared to the control group, but not significant. In the experiment of PFC and HY, the results of experiment groups which was given each samples were significantly higher than the control group. The result of rosette forming cell in GME and GAE groups were significantly higher than control group.

감초 추출물이 면역응답에 미치는 영향

심호기,백반석,황성원,배만종 경산대학교 생명자원개발연구소 1996 생명자원과 산업 Vol.1 No.-

감초에서 분리한 각각의 추출물(HWE, ME, AE)을 BALB/c mouse에 투여하여 탐식작용, 용혈반형성, 용혈소 역가특정 및 Rosette형성 실험을 통해 감초가 면역응답에 미치는 영향을 조사하였다. 1. 탐식능 측정에서 복강침출세포(PEC)와 비장세포(spleen cell)는 CON군에 비하여 가각의 약물투여군이 Candida parapsilosis에 대한 효과가 약간 높은 경향을 나타냈지만 유의성은 없었다. 2. 용혈반 형성 및 용혈소 역가측정에서는 CON군에 비하여 HWE, ME, AE투여 군이 유의성을 나타냈다. 3. Rosette형성에 대한 측정에서는 CON군에 비하여 ME, AE투여 군이 높은 유의성을 나타냈다. 본 실험 결과에서 감초 추출물들은 Candida paraprapsilosis에 대하여 약간의 탐식증진효과를 나타냈으며, 항체생성세포의 용혈반 시험 및 용혈소 역가측정과 Rosette형성측정에서의 유의성을 나타낸 것은 감초가 세포성 면역을 주도하는 T세포를 활성화시킬 뿐만 아니라, 체액성 면역을 주도하는 B세포를 활성화시킨다는 것을 확인 할 수 있다. The inverstigation of effecting immune response on BALB/c mouse was experimented with hot water extract(HWE), 50% methanol extract(ME) and aaceton extract(AE) 〔which was extracted from Glycyrrhiza glabra〕. The experiment was carried out by phagocytosis, hemolytic plaque assay, hemolysin titration and rosette assay. Peritoneal exudative cells and spleen cells in phagocytosis ability; the experimental group has shown a slight increase in Candida parapsilosis(HWE, ME & AE). The experiment shows no relevant correlation. The experiment of hemolytic plaque assay and hemolysin titration was treated by HWE, ME and AE. The experimental group was treated by ME and AE through the measurement of rosette assay. The experimental group has given significant increase compared with the control. As the result, the extracts of Glycyrrhiza glabra (HWE, ME and AE) has effedted slightly to Cnadida parapsilosis in phagocytosis. The reason of effecting the immunocyte in hemolysin titration, rosette assay and hemolytic plaque assay ; Glycyrrhiza glabra has activated the T-cell which controlled cell mediated immunity. Same with B-cell which could also control humoral immunity.

레지오넬라 폐렴의 진단용 바이오마커의 발굴: A/J 마우스 감염 모델에서 Legionella pneumophila의 독력 유전자들의 발현양상 분석

김승민,심희선,김희남,심호기,윤영경,김정연,박윤선,박대원,손장욱,김민자 대한감염학회 2010 Infection and Chemotherapy Vol.42 No.1

Background: Legionella pneumophila is the causative agent of Legionnaires’ disease, a severe form of pneumonia. After L. pneumophila is inhaled through contaminated aerosols, it is phagocytized by alveolar macrophages, multiplies in a specialized phagosome approximately 10 h postinfection, and eventually leads to the death of host cells. Currently available diagnostic tests for Legionella pneumonia have some limitations. This study was conducted to find diagnostic biomarkers for Legionella pneumonia using virulence gene expression profiling in a murine experimental model. Materials and Methods: A/J mice were intranasally inoculated with L. pneumophila serogroup 1, and lungs were harvested 4, 8, 24, and 48 h postinfection. The strain grown in buffered yeast extract broth was used as reference samples. Cy-dye labeled cDNA samples were prepared with total RNA from lungs or broth culture, and hybridized on the oligo-microarray slide containing 2,895 genes of L. pneumophila serogroup 1. Virulence gene expression patterns were analyzed using a MIDAS software from TIGR (www. tigr.org). Results: Among a total of 332 virulence genes examined, 17 genes including sidA, lepB, the genes related to flagella assembly (fliR and fliP), LPS lipid A biosynthesis, and the enhanced entry protein EnhA were up-regulated at all four time points. We further confirmed by quantitative real-time reverse transcription PCR that the expression of fliP gene was highly expressed in lung tissue as well as in bronchoalveolar lavage fluids from the mouse infected with L. pneumophila serogroup 1. Conclusions: Through gene expression analysis of L. pneumophila in a mouse model, several candidate biomarkers for diagnosing Legionnaires’ disease could be identified. Background: Legionella pneumophila is the causative agent of Legionnaires’ disease, a severe form of pneumonia. After L. pneumophila is inhaled through contaminated aerosols, it is phagocytized by alveolar macrophages, multiplies in a specialized phagosome approximately 10 h postinfection, and eventually leads to the death of host cells. Currently available diagnostic tests for Legionella pneumonia have some limitations. This study was conducted to find diagnostic biomarkers for Legionella pneumonia using virulence gene expression profiling in a murine experimental model. Materials and Methods: A/J mice were intranasally inoculated with L. pneumophila serogroup 1, and lungs were harvested 4, 8, 24, and 48 h postinfection. The strain grown in buffered yeast extract broth was used as reference samples. Cy-dye labeled cDNA samples were prepared with total RNA from lungs or broth culture, and hybridized on the oligo-microarray slide containing 2,895 genes of L. pneumophila serogroup 1. Virulence gene expression patterns were analyzed using a MIDAS software from TIGR (www. tigr.org). Results: Among a total of 332 virulence genes examined, 17 genes including sidA, lepB, the genes related to flagella assembly (fliR and fliP), LPS lipid A biosynthesis, and the enhanced entry protein EnhA were up-regulated at all four time points. We further confirmed by quantitative real-time reverse transcription PCR that the expression of fliP gene was highly expressed in lung tissue as well as in bronchoalveolar lavage fluids from the mouse infected with L. pneumophila serogroup 1. Conclusions: Through gene expression analysis of L. pneumophila in a mouse model, several candidate biomarkers for diagnosing Legionnaires’ disease could be identified.

레지오넬라 폐렴의 진단용 바이오마커의 발굴 : A/J 마우스 감염 모델에서 Legionella pneumophila의 독력 유전자들의 발현양상 분석

김승민,심희선,김희남,심호기,윤영경,김정연,박윤선,박대원,손장욱,김민자 대한감염학회 2010 감염과 화학요법 Vol.42 No.1

Background: Legionella pneumophila is the causative agent of Legionnaires’ disease, a severe form of pneumonia. After L. pneumophila is inhaled through contaminated aerosols, it is phagocytized by alveolar macrophages, multiplies in a specialized phagosome approximately 10 h postinfection, and eventually leads to the death of host cells. Currently available diagnostic tests for Legionella pneumonia have some limitations. This study was conducted to find diagnostic biomarkers for Legionella pneumonia using virulence gene expression profiling in a murine experimental model. Materials and Methods: A/J mice were intranasally inoculated with L. pneumophila serogroup 1, and lungs were harvested 4, 8, 24, and 48 h postinfection. The strain grown in buffered yeast extract broth was used as reference samples. Cy-dye labeled cDNA samples were prepared with total RNA from lungs or broth culture, and hybridized on the oligo-microarray slide containing 2,895 genes of L. pneumophila serogroup 1. Virulence gene expression patterns were analyzed using a MIDAS software from TIGR (www.tigr.org). Results: Among a total of 332 virulence genes examined, 17 genes including sidA, lepB, the genes related to flagella assembly (fliR and fliP), LPS lipid A biosynthesis, and the enhanced entry protein EnhA were up-regulated at all four time points. We further confirmed by quantitative real-time reverse transcription PCR that the expression of fliP gene was highly expressed in lung tissue as well as in bronchoalveolar lavage fluids from the mouse infected with L. pneumophila serogroup 1. Conclusions: Through gene expression analysis of L. pneumophila in a mouse model, several candidate biomarkers for diagnosing Legionnaires’ disease could be identified.

기능성 식품 자원의 지질, 아미노산 및 식이 섬유의 조성 - 길경, 들깨 종자, 달맞이꽃 종자, 알로에 베라 -

황성원(Sung-Won Hwang),박무희(Moo-Hee Park),심호기(Ho-Ki Shim),배만종(Man-Jong Bae) 한국식품영양과학회 1994 한국식품영양과학회지 Vol.23 No.4

길경, 들깨 종자, 달맞이꽃 종자, 알로에 베라 등의 식이 섬유소, 지방산 조성 및 염용성 단백질의 아미노산을 분석 비교한 결과는 다음과 같다. 식이 섬유로서 neutral detergent fiber(N.D.F), acid detergent fiber(A.D.F), lignin, hemicellulose, cellulose 등의 함량은 달맞이꽃 종자가 cellulose를 제외하고는 많은 함량이었다. 총지질의 지방산조성에서 P/S 비율은 들깨 종자가 6.31로 높았으며, linolenic acid (n-3)는 들깨 종자에 있어 55.47%, linoleic acid (n-6)는 달맞이꽃 종자에 71.88%로 그 함유비가 높았다. 중성지질의 지방산 조성은 총지질의 지방산 조성과 비슷하였다. 당지질의 지방산 조성에서 PUFA의 함유비는 들깨 종자 61.76%, n-6/n-3 비율은 달맞이꽃 종자가 15.19이었다. 인지질의 지방산 조성은 당지질의 지방산과 조성과 비슷하며, 길경의 PUFA의 함량은 62.96%로 약간 증가되었다. 염용성 단백질의 아미노산 조성에서 필수아미노산은 길경이 47 ㏖e%로 높은 함유비를 나타내었으며, E/N 비율은 길경이 0.9, 알로에 베라가 0.66이었다. This study was conducted to investigate the contents of dietary fiber(DF), compositions of fatty acids in lipid fraction and amino acids in salt-soluble protein from the functional food sources such as Platycodi radix, perilla seed, evening primrose seed and aloe vera. The contents of dietary fiber, neutral detergent fiber (N.D.F), acid detergent fiber (A.D.F), lignin, hemicellulose and cellulose in evening primrose seed were higher than those of other samples, except the content of cellulose. The ratio of polyunsaturated/saturated (P/S) fatty acid in total lipids was 6.31 in perilla seed, which was higher than those of other samples. The content of linolenic acid (n-3) in perilla seed was 55.47%. The content of linoleic acid (n-6) in evening primrose seed was 71.88%, which was higher than those of other samples. The fatty acid compositions in neutral lipids were the same as those of total lipids. The PUFA contents of fatty acid in glycolipids were 61.76% in perilla seed. And also, the ratio of n-6/n-3 in evening primrose seed was 15.19. The fatty acid compositions in phospholipids were the same as those of glycolipids. The contents of PUFA in Platycodi radix were 62.96%. The essential amino acid contents of salt-soluble protein were 47mole% in Platycodi radix, which was slightly higher than those other samples. The ratio of essential amino acid / nonessential amino acid (E/N) was 0.9 and 0.66 in Platycodi radix and aloe vera, respectively.