Plant regeneration from callus of Iris odaesanensis Y. N. Lee native to Korea via organogenesis

Bae, Kee-Hwa,Yoo, Kyoung-Hwa,Lee, Mi-Hyun,Jeong, Jae-Hun,Choi, Yong-Eui,Yoon, Eui-Soo The Korean Society of Plant Biotechnology 2013 식물생명공학회지 Vol.40 No.3

Iris odaesanensis Y. N. Lee. is an important endangered and native plant belonging to the family Iridaceae in Korea. This study describes a method for rapid micropropagation of this species via from leaf, rhizome and root explants derived calli. Leaf, rhizome and root explants were cultured on Murashige and Skoog (MS) medium supplemented with 2,4-dichlorophenoxy acetic acid (2,4-D) for callus induction. Rhizome explants yielded calli at a frequency of 72% when cultured at 1.0 mg/l 2,4-D. Calli were maintained at 1.0 mg/l 2,4-D. These calli were transferred to MS medium supplemented with 0, 0.5, 1.0, and 2.0 mg/l 2,4-D in combination with 0, 0.5, 1.0, and 3.0 mg/l BA for adventitious shoot induction. The highest number of adventitious shoot (228.9 per petri-dish) were formed at 1.0 mg/l 2,4-D and 1.0 mg/l BA. WPM medium was the best to convert calli into plantlets, where up to 98.2% of calli were regenerated into plantlets. This in vitro propagation protocol should be useful for conservation of this endangered plant.

전측 시개전핵의 하행성 통각 조절계에 대한 하올리브 수준의 복측 연수의 관여

이배환,홍승길,안창일,손진훈,김기석 한국심리학회 한국심리학회지 생물 및 생리 Vol.6 No.1

본 연구는 전측 시개전핵의 자극으로 인한 하행성 통각 억제 체계에 하올리브 수준의 복측 연수가 관여하는가를 알아보기 위해 수행되었다. 펜토바비탈로 마취된 쥐의 꼬리튀기 반사를 사용하여 전측 시개전핵을 전기자극하고 복측 연수를 전기손상시키거나 리도카인을 주입한 결과 전측 시개전핵의 자극으로 유발된 무통 효과가 억제되었다. 반대로 복측 연수를 전기자극하거나 글루타메이트를 주입하여 세포체를 활성화시키면 마찬가지로 무통이 발생하였다. 이 결과는 전측 시개전핵의 하행성 통각 억제 체계에 하올리브 수준의 복측 연수가 관여한다는 것을 시사한다. The present study was conducted to determine whether the ventral medulla (VM) at the level of the inferior olive (IO) is involved in descending pain inhibition system including the anterior pretectal nucleus (APTN). Pain sensitivity was assessed using tail-flick test to radiant heat in the rat anaesthetized with pentobarbital. Electrolytic lesions of the VM or microinjections of lidocaine into the VM inhibited the analgesic effects of stimulating the APTN. Electrical stimulation of the VM or microinjections of glutamate into the VM produced analgesic effects similar to those of stimulating the APTN. These results suggest that the VM including the IO is involved in a descending antinociceptive pathway originating in the APTN.

열대지방사람들의 땀샘의 밀도와 기능

이정범,배준상,민영기,양훈모 순천향의학연구소 2004 Journal of Soonchunhyang Medical Science Vol.10 No.1

Tropical inhabitants are able to tolerate heat through permanent residence in the tropics. To clarify the peripheral mechanisms of the suppressed thermal sweating in tropical natives, sweating responses to acetylcholine (ACh), a primary transmitter of the sudomotor innervation, were compared between healthy Korean and African males. ACh was iontophoretically administered on the forearm. Directly activated and axon reflex-mediated sweat responses were evaluated by quantitative sudomotor axon reflex test (QSART). The sweating of axon reflex (nicotinic receptor) onset-time was 0.42 min shorter (P<0.01) and the sweat volumes (muscarinic receptors) were 75% (P<0.01) and the ACh sweat volumes of axon reflex (nicotinic receptor) 79% higher (P<0.01) in the Koreans than the Africans. Iodine-impregnated paper method revealed that sweat gland density was 45.1% higher (P<0.01) and sweat gland output per single gland was 18.4% larger (P<0.001) in the Koreans compared to the Africans. These results indicate that suppressed thermal sweating in Africans is, at least in part, attributed to the suppressed glandular sensitivity to ACh through both recruitment of sweat glands and sweat output per each gland.

미세혈관 문합술을 이용한 절단된 귀 재접합 치험례:1례보고

김정헌,이현택,박배근,김동철,황성진 대한성형외과학회 1992 Archives of Plastic Surgery Vol.19 No.3

The auricle is frequently exposed to trauma due to its protruding anatomical position and it is sometimes severed completely. Many kinds of techniques such as composite graft or cartilage preservation methods for the reattachment or reconstruction of and amputated ear have been tried. But their indications are limited and results are not satisfactory. For the complete survival of ear cartilage and skin of the amputated part, microvascular replantation is known as the best procedure. But cases of successful replantations have been rare until now, because of technical difficulties due to the small size of auricular vessels and uncertain vascular anatomy. In 1980, Pennington et al. reported the first successful microvasclar replantation of a totally avulsed ear using vein graft. After that about 10 cases of world wide successful micro replantation of servered ears were reported. Here, we report a case of a successful replantation of a completely amputated lower half of auricle including the ear lobule due to a human bite. Replantation was done by direct anastomosis of transected peripheral branches of the posterior auricular artery and vein without vein graft. We again emphasize that, in spite of the technical difficulties and long operative time, successful replantation of an amputated ear with microvascular surgery is superior to any other methods of reattachment or reconstruction.

Clinical significance of HIF-2α immunostaining area in radioresistant cervical cancer (초)

김민규 ( Min Kyu Kim ),( Tae Joong Kim ),( You Young Lee ),( Chel Hun Choi ),( Jeong-Won Lee ),( Duk Soo Bae1 ),( Je Ho Lee ),( Byoung Gie Kim ) 대한산부인과학회 2010 대한산부인과학회 학술대회 Vol.96 No.-

Hepatoprotective and Antioxidative Activities of <i>Cornus officinalis</i> against Acetaminophen-Induced Hepatotoxicity in Mice

Lee, Nam-Hun,Seo, Chang-Seob,Lee, Ho-young,Jung, Da-Young,Lee, Jun-Kyung,Lee, Jin-Ah,Song, Kye Yong,Shin, Hyeun-kyoo,Lee, Mee-Young,Seo, Young Bae,Kim, Hokyoung,Ha, Hyekyung Hindawi Publishing Corporation 2012 Evidence-based Complementary and Alternative Medic Vol.2012 No.-

<P>The fruit of <I>Cornus officinalis </I>Sieb. et Zucc. is commonly prescribed in Asian countries as a tonic formula. In this study, the hepatoprotective effect of ethanolic extracts of the fruit of <I>C. officinalis</I> (ECO) was investigated in a mouse model of acetaminophen- (APAP-) induced liver injury. Pretreatment of mice with ECO (100, 250, and 500 mg/kg for 7 days) significantly prevented the APAP (200 mg/kg) induced hepatic damage as indicated by the serum marker enzymes (AST, ALT, and LDH). Parallel to these changes, ECO treatment also prevented APAP-induced oxidative stress in the mice liver by inhibiting lipid peroxidation (MDA) and restoring the levels of antioxidant enzymes (SOD, CAT, and HO-1) and glutathione. Liver injury and collagen accumulation were assessed using histological studies by hematoxylin and eosin staining. Our results indicate that ECO can prevent hepatic injuries associated with APAP-induced hepatotoxicity by preventing or alleviating oxidative stress.</P>

DNA barcoding techniques for avian influenza virus surveillance in migratory bird habitats.

Lee, Dong-Hun,Lee, Hyun-Jeong,Lee, Youn-Jeong,Kang, Hyun-Mi,Jeong, Ok-Mi,Kim, Min-Chul,Kwon, Ji-Sun,Kwon, Jun-Hun,Kim, Chang-Bae,Lee, Joong-Bok,Park, Seung-Yong,Choi, In-Soo,Song, Chang-Seon [Wildlife Disease Association] 2010 JOURNAL OF WILDLIFE DISEASES Vol.46 No.2

<P>Avian influenza virus (AIV) circulates among free-ranging, wild birds. We optimized and validated a DNA barcoding technique for AIV isolation and host-species identification using fecal samples from wild birds. DNA barcoding was optimized using tissue and fecal samples from known bird species, and the method was shown to distinguish 26 bird species. Subsequently, fecal samples (n=743) collected from wild waterfowl habitats confirmed the findings from the laboratory tests. All identified AIV-positive hosts (n=35) were members of the order Anseriformes. We successfully applied the DNA barcoding technique to AIV surveillance and examined AIV epidemiology and host ecology in these wild waterfowl populations. This methodology may be useful in the design of AIV surveillance strategies.</P>

運動選手의 身體構成 評價를 위한 體密度, 體脂肪率 推定式의 交叉妥當化 檢證

강상조,육현철,정현택,원신희,이승국,이병두,이미영,안효작,김기범,김광배,신범철,전승훈 韓國體育大學校附屬 體育科學硏究所 1995 韓國體育大學校附屬 體育科學硏究所論文集 Vol.14 No.1

The purpose of this study was to evaluate the body fat percent (%Fat) value from nine selected skinfold (SF) equations against a criterion value from hydrostatic weighing (HW) in male althetes.Nine SF equations were cross-validated in four different athletic events (n=73). Criterion percent body fat was determined by HW and computed from body density (BD) for a mixed sample of all event players (T), Swimming (SW: n=14), Taekondo (TA: n=24), Weight lifter (WL: n=l8), and Judo (JU: n=17). Results were analyzed by events using t-test. Analysis included computation of mean different (MD), correlation (r), standard error of estimation (SEE), total error (TE). Skinfolds had MDs ranging from -6.5%∼.19%(T), -3.25%~ -12.51% (SW), -4.98%~ .57% (TA). -4.3%~2.66% (WL), -6.1%~9.17% (JU), correlations (r) from .474~.563 (T), .567~.641(SW), .382-.589 (TA), .772 ~.872 (WL), .772-.821 (JU), SEEs from 3.47% ~ 3.6% (T), 2.68% ~ 2.88% (SW), 3.O2%~3.46% (TA) 1.9%~2.65% (WL), 2.9%~ 3.23%(JU), and TEs .7%~ 6.52%(T), 3.55%~12.51% (SW), 2.59%~5.02% (TA), 1.82%~ 4.5% (WL), 2.22%~ 6.06% (JU). The Kang, Lee, Lee, Cho (1994) and Kang, Lee, Lee (1995) SF equation provided the most valid prediction of HW determined %Fat in WL, demonstrating the highest correlation, lowest SEE and TE, and intercept and slope nearly closed 0.0 and 1.0, respectively.

Structural Characterization of HP1264 Reveals a Novel Fold for the Flavin Mononucleotide Binding Protein

Lee, Ki-Young,Kim, Ji-Hun,Lee, Kyu-Yeon,Lee, Jiyun,Lee, Ingyun,Bae, Ye-Ji,Lee, Bong-Jin American Chemical Society 2013 Biochemistry Vol.52 No.9

<P>Complex I (NADH-quinone oxidoreductase) is an enzyme that catalyzes the initial electron transfer from nicotinamide adenine dinucleotide (NADH) to flavin mononucleotide (FMN) bound at the tip of the hydrophilic domain of complex I. The electron flow into complex I is coupled to the generation of a proton gradient across the membrane that is essential for the synthesis of ATP. However, <I>Helicobacter pylori</I> has an unusual complex I that lacks typical NQO1 and NQO2 subunits, both of which are generally included in the NADH dehydrogenase domain of complex I. Here, we determined the solution structure of HP1264, one of the unusual subunits of complex I from <I>H. pylori</I>, which is located in place of NQO2, by three-dimensional nuclear magnetic resonance (NMR) spectroscopy and revealed that HP1264 can bind to FMN through UV–visible, fluorescence, and NMR titration experiments. This result suggests that FMN-bound HP1264 could be involved in the initial electron transfer step of complex I. In addition, HP1264 is structurally most similar to <I>Escherichia coli</I> TusA, which belongs to the SirA-like superfamily having an IF3-like fold in the SCOP database, implying that HP1264 adopts a novel fold for FMN binding. On the basis of the NMR titration data, we propose the candidate residues Ile32, Met34, Leu58, Trp68, and Val71 of HP1264 for the interaction with FMN. Notably, these residues are not conserved in the FMN binding site of any other flavoproteins with known structure. This study of the relationship between the structure and FMN binding property of HP1264 will contribute to improving our understanding of flavoprotein structure and the electron transfer mechanism of complex I.</P><P><B>Graphic Abstract</B> <IMG SRC='http://pubs.acs.org/appl/literatum/publisher/achs/journals/content/bichaw/2013/bichaw.2013.52.issue-9/bi301714a/production/images/medium/bi-2012-01714a_0007.gif'></P><P><A href='http://pubs.acs.org/doi/suppl/10.1021/bi301714a'>ACS Electronic Supporting Info</A></P>

Molecular Characterization of a Chinese Cabbage cDNA Encoding Thioredoxin - h that is Predominantly Expressed in Flowers

Lee, Sang Yeol,Lee, Kyun Oh,Jang, Ho Hee,Lee, Ji Yeun,Lee, Seung Sik,Jung, Bae Gyo,Chi, Yong Hun,Kang, Soon Suk,Park, Soo Kwon,Lee, Jung Ro,Yoo, Ji Young 생화학분자생물학회 1970 BMB Reports Vol.34 No.4

Even though three isotypes of thioredoxins (-f, -m and -h types) have been identified in a variety of plant cells, there are only a few reports on thioredoxin-h that were recently identified. In this study, a cDNA encoding a h-type of thioredoxin was isolated from a cDNA library of Chinese cabbage, and named here CTrx-h. An open reading frame of the gene contained a polypeptide of 133 amino acids with a conserved active center, WCGPC, which appeared in all of the thioredoxin proteins. A deduced amino acid sequence of the CTrx-h showed the highest sequence identity with those of Arabidopsis thioredoxin-h2 (75.2 %) and thioredoxin-h5 (46.6%) proteins, but it shared a low sequence homology to other isotypes of plant thioredoxinm and thioredoxin-f. The CTrx-h protein that is expressed in E. coli represented not only an insulin reduction activity, but also electron transferring activity from NADPH to thioredoxin-dependent peroxidase. A genomic Southern blot analysis using the cDNA insert of CTrx-h revealed that the gene consisted of a small multigene family in Chinese cabbage genome. On the contrary to other thioredoxin-h proteins that were widely distributed in most tissues of the plant, the CTrx-h was predominantly expressed in flowers. The expression was very low in other tissues. The data of the Northern blot analysis suggests that the CTrx-h may have other functions in flower development or differentiation, in addition to its defensive role.