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홍수종,심정연,조유숙,박재경,우빈,문희범 대한천식알레르기학회 1997 천식 및 알레르기 Vol.17 No.3
To investigate the imbalance of the cytokine production profile of T cells from atopic asthmatics, we measured concentrations of IL - 4, IL - 5 and IFN-r by ELISA method in the culture supernatants of peripheral blood mononuclear cells(PBMCs) and Dermato- phagoides pteronyssinus(Der p) 1 stimulated PBMCs from Der p sensitized atopic asthmatics, Der p sensitized.healthy atopics, non atopic asthmatics and healthy non atopics. The suppressive effect of IFN-r on cytokine production of Der p 1 stimulated PBMCs was also examined. The PBMCs from atopics showed higher IL 4 and IL 5 production in response to PHA +TPA and higher IFN-r production in response to Der p lq compared with non atopics. The Der p 1 stimulated PBMCs from atopics showed a tendency of increased IL 5 production in response to Der p 1 and higher IL 4 and IL 5 production in response to PHA+TPA compared with non atopics. IL 5 production of Der p 1 stimulated PBMCs from atopics was suppressed by IFN - r It is suggested t,hat an imbalance in IL 4, IL 5 and IFN -r production is a feature of the atopic state. The TH characteristics of allergen stimulated PBMCs could be regulated by IFN -r .
천식 환자에서 분리된 Dermatophagoides pteronyssinus 항원 - 특이 T세포 클론의 특성
홍수종,오순환,조영주,유빈,고윤석,문희범 대한천식알레르기학회 1995 천식 및 알레르기 Vol.15 No.1
T cells play a central role in IgE synthesis and airway inflammation in asthmatic patients. In order to characterize antigen specific T cell clones, we established Dermatophagoides pteronyssinus(D.p) specific and purified protein de- rivatives(PPD) -specific T cell clones, and investigated their cytokine production profile in response to the antigenic and nonspecific stimulation. We established six D.p-specific T cell clones from the peripheral blood of two atopic asthmatics and seven PPD-specific T cell clones from one PPD sensitized ayrnptomatic individual and two pulmonary tuberculosis pa- tients using limiting dilution method. We analyzed the ability of T cell clones to produce IL 4, IFNy and their IL 2R expres- sion upon stimulation with D.p, PPD or PHA plus TPA. Only one D.p-specific T cell clone produced IL 4 upon stimulation with D.p, whereas three clones produced both IL 4 and IFNy upon stimulation with PHA plus TPA, Four PPD specific T cell clones produced IFNy upon stimulation with PPD, five clones produced IFNy upon stimulation with PHA plus TPA, but none of them produced IL-4 regardless of stimulation. Two of five D.p-specific T cell clones and one of four PPD-specific T cell clones showed more than 200 % increass in IL 2R expression upon stimulation with each antigen. These data showed that some of D.p-specific T cell clones were able to produce both IL 4 and IFNy after nonspecific stimulation, but PPD specific T cell clones produced only IFNy regardless of stimulation. This suggests that D. p-specific T cell clones have the ability to produce TH, type cytokines depending on the stimulation.
Interleukin 4와 hydrocortosone에 의한 아토피 환자 말초혈액 단핵구의 IgE 생산조절
조영주,홍수종,문희범 대한천식알레르기학회 1997 천식 및 알레르기 Vol.17 No.4
Glucocorticoid hormones have been identified as one of the B cell activating signals necessary in IgE synthesis in the presence interleukin 4(IL-4). One question to be addressed in IgE synthesis is whether there is difference between atopics and non-atopics. In the present study, we aimed at looking the different effects of interleukin 4(IL-4) and hydrocortisone(HC) in IgE synthesis by peripheral blood monounclerar cells (PBMCs) from 12 atopic patients and 6 non-atopic centrols. PBMCs were cultured with IL-4 and/or HC for 14 days, and net IgE production was measured in the supernatant. Significant spon- taneous IgE production by PBMCs was oberserved only in atopics. IL-4 increased net IgE synthesis by PBMCs from both atopics and non-atopics by similar amounts, whereas HC had that effect only in some atopics who showed high spontaneous IgE production. HC acted synergically with IL-4 in a narrow range of concentration which is individually different. This effect was more remarkable in subjects with low total serum IgE levels. These data suggest that atopic patients may have larger numbers of B cells committed to produce IgE, and that the effect of HC on IgE synthesis in vitro may be due to the priming effect of IL-4 in vivo.
문수영,임민규,홍수지,한민제,송상훈,임경수,유경상,장인진,이지원,강형진,송정한,전용범 대한진단검사의학회 2014 Annals of Laboratory Medicine Vol.34 No.1
Background: Busulfan, an alkylating agent administered prior to hematopoietic stem cell transplantation, has a narrow therapeutic range and wide variability in metabolism. We developed a liquid chromatography-tandem mass spectrometry (LC-MS/MS) method for rapid and accurate quantification of plasma busulfan. Methods: Busulfan was separated and detected using an LC system containing a C18 column equipped with MS/MS. The sample was eluted with a mobile phase gradient for a total run time of 10 min. Plasma busulfan concentration was quantified against a 6-point standard curve in a multiple reaction monitoring mode at mass-to-charge ( m/z ) 264.1 > 151.1. Precision, recovery, matrix effect, linearity, detection capability, carryover, and sta- bility were evaluated. The range of plasma busulfan concentration was obtained by ana- lyzing samples from 9 children receiving busulfan. Results: The coefficients of variation of within-run and within-laboratory precision were all below 5%. Recoveries were all within the range of 100-105%. Linearity was verified from 0 to 5,000 ng/mL. Limit of detection and limit of quantification were 1.56 and 25 ng/mL, respectively. Carryover rate was within allowable limits. Plasma busulfan concentration was stable for 2 weeks at -20°C and -80°C, but decreased by 25% when the plasma was stored for 24 hr at room temperature, and by <5% in 24 hr at 4°C. The plasma busulfan concentrations were between 347 ng/mL and 5,076 ng/mL. Conclusions: Our method using LC-MS/MS enables highly accurate, reproducible, and rapid busulfan monitoring with minimal sample preparation. The method may also enable safe and proper dosage.
집먼지진드기 항원 - 특이 T - 세포 클론에서 추가 자극에 따른 IL - 4 , IL - 5 , IFN - r mRNA 발현양상의 변화
조상헌(Sang Heon Cho),홍수종(Soo Jong Hong),김윤근(Yoon Keun Kim),박재경(Jae Kyung Park),심정연(Jung Yeon Shim),문희범(Hee Bom Moon),민경업(Kyoun Up Min),김유영(You Young Kim) 대한천식알레르기학회 1998 천식 및 알레르기 Vol.18 No.4
Background: It is known that immunotherapy promotes the development of allergen-specific Thl-like lymphocytes whose products are effective in inhibiting clinical response of sensitized atopic patients to allergen exposure. At the single cell level in short term culture, however, IL-4 and IL-5 are co-expressed, while IL-4 and IFN-y are exclusively expressed. Objective. IL-4, IL-5, and IFN-y mRNA were measured in Der pI-specific T-cell clones (TCCs) to evaluate whether expression of cytokine in allergen-specific TCC is fixed regardless of stimuli. Methods: Seven Der pI-specific TCCs were made from two asthmatics sensitive to D. pteronyssinus. IL-4, IL-5, and IFN-y mRNA were measured by RT-PCR in these TCCs after antigen-specific (Der pI) and nonspecific (PHA + TPA) stimuli. Results: IL-4 and IL-5 mRNA were expressed in four and six of seven TCCs, but IFN-y mRNA was not expressed in any TCCs after Der pI-specific stimuli. Meanwhile, after the stimulus of TPA plus PHA, IFN-y mRNA as well as IL-4 and IL-5 mRNA were expressed in four of seven TCCs, and in one TCC, only IFN-y mRNA was expressed without expression of IL-4 mRNA. Conclusion: The expression of cytokine may be variable in allergen-specific TCC according to the type and amount of stimuli.