사업신탁의 현황 및 활성화 방안

한지유 사단법인 한국신탁학회 2022 신탁연구 Vol.4 No.2

The complete revision of Korea trust law in 2011 led to a change of the scope of possible trust to "property," not "property right". Accordingly, not only a part of business, an intellectual property right, security right and including debts as well as positive properties can be considered trust fund. And plus, securitization of beneficiaries’ interests of a trust and a trust where the trustee’s personal liability is limited and others have been accepted and the trust system can be used as the types of corporate organization, it is expected to utilize business trust. Concerning the meaning of 'business trust', there has no been the regulation of definition, in the current laws but a general interpretation of it is given, according to requirements, through precedents and other laws. Whether business trust includes the civil trust or only commercial trust is determined by the definition of business trust. Business trust has the function to Insolvency protection and limited liability, plus, operating enterprises as a form of business organization. The recent years have witnessed the active use of business trust that replaces or complements the existing company systems. The patterns of the use of business trust can be investigated as follows. For business trust, there is a case where a part of business itself would be done as trust fund, and another case where business would be indirectly done through corporate control. There are, also, the detailed types of a trust in which another person becomes a beneficiary and the declaration of trust. The case where business would be indirectly done through corporate control is done, in the terms of corporate organization act corresponds to the case where a trustee owns company stocks and governs the company, in the position of stockholder. Therefore, it is important to clarifying the meaning of stock and a stockholder's right. If we can accept the separation between stock's economic interests and voting rights, the dual class voting right and voting trust that has recently been at issue can be allowed. In the case of Singapore, the Business Trust Act was enacted as part of institutional preparations to become a global financial advanced country. This country is recognized as a leading country in business trusts as it is currently used in various company forms due to various operating methods and flexible dividend systems. Meanwhile in the case of Korea, the existing「Business Trust Act」was incorporated into 「FINANCIAL INVESTMENT SERVICES AND CAPITAL MARKETS ACT」(hereinafter refers to 'Capital Markets Act') which was constituted as No. 8635 of the legislation, on Aug. 03, 2007. Accordingly, if the revision of the Trust Act and the provisions of the Capital Markets Act are inconsistent, existing legal restrictions are maintained by the Capital Markets Act or it is difficult to prepare unified guidelines for the business trust system, making it inconvenient on using the system. In order to vitalize business Trust, this study is going to consider legislative and institutional improvement methods about Trust Act and Capital Market Act. The legislative improvement methods about Trust Act would be developed by ① separating Business Trust Act from Capital Markets Act. ② the explicit standard of detailed classification between Trust and Business Trust is to be set. ③ The exception provision of Article 36 of Trust Act that bans a trustee from becoming a sole beneficiary is to be accepted. The legislative improvement measures about Capital Markets Act would be pursued by ① allowing the exception provision of Clause 1 of Article 112 of Capital Market Act that prohibits the exercising of voting rights when a trustee exceeds 15% of owned stocks, ② Exceptions to Clause 1 of Article 3 of Capital Markets Act that prohibits issuing beneficiary certificates of 'managerial trust' are allowed. and ③ by allowing to issue beneficiary certificates by business trust and applying re...

세포배양육 연구개발 산업계 동향

한지유(Jiyou Han),신재관(Jaekwan Shin),지현근(Hyeon-Gun Jee) 한국축산식품학회 2021 축산식품과학과 산업 Vol.10 No.1

Recent Advances for Enhancing Drug Metabolizing Functions of Hepatocyte-like Cells Derived from Human Pluripotent Stem Cells

김종훈,한지유 한양대학교 의과대학 2015 Hanyang Medical Reviews Vol.35 No.4

Hepatocyte-like cells (HLCs) derived from human pluripotent stem cells are a promising cell source for drug screening and toxicity tests. Thus, various hepatic differentiating protocols have been developed, leading to a hepatic differentiation efficiency of approximately 90%. However, HLC drug metabolizing ability remains very low compared to human primary hepatocytes. In order to overcome this problem, several alternative methods, such as, co-culture, three-dimensional (3D) culture, bioreactor, nanochip-based, etc., have been developed, but optimization to produce fully functional HLCs is ongoing. Recently, our group reported that repeated exposure of HLCs to xenobiotics can improve the expression of hepatic metabolizing enzymes such as cytochrome P450s (CYPs) and glutathione S-transferases (GSTs). These data suggest that we should develop strategies for differentiating cells into mature HLCs by more closely mimicking in vivo fetal and postnatal liver development. Here, we review the current development of alternative methods for enhancing the drug metabolizing functions of HLCs derived from human embryonic stem cells, human-induced pluripotent stem cells, and mesenchymal stem cells as used for drug screening and toxicity tests.

Fetal hematopoietic stem cells express MFG-E8 during mouse embryogenesis

이재훈,최병일,박서영,안수연,한지유,김종훈 생화학분자생물학회 2015 Experimental and molecular medicine Vol.47 No.-

the milk fat globule-EGF-factor 8 protein (MFG-E8) has been identified in various tissues, where it has an important role in intercellular interactions, cellular migration, and neovascularization. Previous studies showed that MFG-E8 is expressed in different cell types under normal and pathophysiological conditions, but its expression in hematopoietic stem cells (HSCs) during hematopoiesis has not been reported. In the present study, we investigated MFG-E8 expression in multiple hematopoietic tissues at different stages of mouse embryogenesis. Using immunohistochemistry, we showed that MFG-E8 was specifically expressed in CD34+ HSCs at all hematopoietic sites, including the yolk sac, aorta-gonad-mesonephros region, placenta and fetal liver, during embryogenesis. Fluorescence-activated cell sorting and polymerase chain reaction analyses demonstrated that CD34+ cells, purified from the fetal liver, expressed additional HSC markers, c-Kit and Sca-1, and that these CD34+ cells, but not CD34−cells, highly expressed MFG-E8. We also found that MFG-E8 was not expressed in HSCs in adult mouse bone marrow, and that its expression was confined to F4/80+ macrophages. Together, this study demonstrates, for the first time, that MFG-8 is expressed in fetal HSC populations, and that MFG-E8 may have a role in embryonic hematopoiesis.

리튬2차전지에서 SiO2 sol로부터 제조된 SiOx/graphite anode의 충방전 특성

문대일,이재호,오윤경,한지유,김지수,박경세,선호정,심중표 한국공업화학회 2011 한국공업화학회 연구논문 초록집 Vol.2011 No.1

리튬2처전지에서 용액공정을 통한 SiOx/Graphite 복합전극의 제조와 충방전 특성 분석

문대일,이재호,오윤경,한지유,김지수,박경세,선호정,심중표 한국공업화학회 2011 한국공업화학회 연구논문 초록집 Vol.2011 No.1

Biochemical and Morphological Effects of Hypoxic Environment on Human Embryonic Stem Cells in Long-Term Culture and Differentiating Embryoid Bodies

Hee-Joung Lim,한지유,Dong-Hun Woo,Sung-Eun Kim,김슬기,강희규,김종훈 한국분자세포생물학회 2011 Molecules and cells Vol.31 No.2

The mammalian reproductive tract is known to contain 1.5-5.3% oxygen (O_2), but human embryonic stem cells (hESCs) derived from preimplantation embryos are typically cultured under 21% O_2 tension. The aim of this study was to investigate the effects of O_2 tension on the long-term culture of hESCs and on cell-fate determination during early differentiation. hESCs and embryoid bodies (EBs) were grown under different O2 tensions (3, 12, and 21% O_2). The expression of markers associated with pluripotency, embryonic germ layers, and hypoxia was analyzed using RT-PCR, immunostaining, and Western blotting. Proliferation, apoptosis, and chromosomal aberrations were examined using BrdU incorporation, caspase-3 immunostaining, and karyotype analysis, respectively. Structural and morphological changes of EBs under different O_2 tensions were comparatively examined using azan- and hematoxylin-eosin staining, and scanning and transmission electron microscopy. Mild hypoxia (12% O_2) increased the number of cells expressing Oct4/Nanog and reduced BrdU incorporation and aneuploidy. The percentage of cells positive for active caspase-3, which was high during normoxia (21% O_2), gradually decreased when hESCs were continuously cultured under mild hypoxia. EBs subjected to hypoxia (3% O_2) exhibited well-differentiated microvilli on their surface, secreted high levels of collagen, and showed enhanced differentiation into primitive endoderm. These changes were associated with increased expression of Foxa2, Sox17, AFP, and GATA4 on the EB periphery. Our data suggest that mild hypoxia facilitates the slow mitotic division of hESCs in long-term culture and reduces the frequency of chromosomal abnormalities and apoptosis. In addition, hypoxia promotes the differentiation of EBs into extraembryonic endoderm.

Importance-Performance Analysis와 Borich 요구도 분석 방법을 활용한 임상간호실무 교육 요구도: 일개 상급종합병원 일반병동 간호사 대상

이미라(Lee, Mira),김지영(Kim, Jiyoung),김보연(Kim, Boyeon),박유연(Park, Yooyun),한지유(Han, Jiyoo),이승희(Lee, Seunghee),이현주(Lee, Hyunju) 한국간호교육학회 2023 한국간호교육학회지 Vol.29 No.2

Purpose: This study’s aim was to confirm the importance and performance of the clinical practice of nurses working in the general wards of a tertiary hospital and to analyze their educational needs. Methods: The study employed a descriptive research design, and a self-reported questionnaire was developed and used for nurses in a tertiary hospital. Data were collected from July 22 to July 29, 2022, analyzed by an independent t-test, paired t-test, and one-way ANOVA for the importance and performance of each clinical nursing practice according to general characteristics, and then a post hoc verification was performed by Scheffé’s test. An Importance-Performance Analysis and Borich needs assessment model were used to analyze clinical nursing education needs. Results: Clinical nursing practice performance showed a significant difference according to length of total clinical career, time working in current department, and preceptor experience. According to the results of the Importance-Performance Analysis, neurological evaluation, and nursing intervention, artificial respirator and high-flow oxygen inhalation nursing, chemotherapy, emergency nursing, and cardiopulmonary resuscitation were identified as belonging to the “concentrate here” quadrant. All these items ranked in the top 10 in the Borich needs assessment model. Conclusion: Based on these results, the current education system should be reviewed, and short and long term education strategies based on educational needs should be established to strengthen the competence of nurses.

암세포 Bcl-2 family 유전자 군의 DNA 메틸화 연구

강영섭 ( Young Suep Kang ),이선영 ( Sun Young Lee ),정상근 ( Sang Gun Jung ),한지유 ( Ji You Han ),고정재 ( Jeong Jae Ko ),배지현 ( Jee Hyeon Bae ),나영정 ( Young Junh Na ),이찬 ( Chan Lee ),목정은 ( Jung Un Mock ),김승조 ( Sung 대한산부인과학회 2007 Obstetrics & Gynecology Science Vol.50 No.7

목적: 본 연구는 암세포주와 난소암 조직에서 세포예정사의 핵심 조절 단백질인 Bcl-2 family 유전자의 DNA 메틸화 여부를 암과의 관련성을 밝히는데 그 목적이 있다. 연구 방법: 자궁경부암 세포주인 HeLa, CaSki 그리고 만성골수성 혈액암 세포주인 K562 세포에서 genomic DNA를 추출하여 sodium bisulfite를 통한 cytosine 염기를 uracil로 치환하였다. 치환된 염기서열은 methylation과 unmethylation을 특이적으로 확인할 수 있는 primer를 이용하여 MSP (Methylation Specific PCR)을 수행한 후 암세포에서 Bcl-2 family 유전자의 DNA 메틸화 여부를 판별하였다. 결과: 각각의 세포주에서 antiapoptotic Bcl-2 family 유전자 군인 Mcl-1과 Bcl-2 유전자는 DNA 메틸화가 이루어지지 않은 것으로 관찰되었으며, proapoptotic Bcl-2 family 구성원인 Harakiri 유전자는 DNA 메틸화가 이루어진 것을 확인하였다. 반면, 다른 proapoptotic Bcl-2 family 유전자인 Noxa 유전자는 DNA 메틸화가 이루어지지 않은 것으로 관찰되었다. 또한 난소암 조직에서의 DNA 메틸화 여부를 살펴본 결과 Mcl-1과 Noxa는 세포주에서와 같은 결과를 보였고, Harakiri 유전자의 경우 hypomethylation으로 관찰되었다. 결론: 본 연구는 암세포주와 난소암 조직에서 proapoptotic Bcl-2 family 유전자인 Harakiri와 Noxa의 경우, 유전자 특이적인 DNA의 메틸화가 나타났으며 antiapoptotic 유전자는 DNA의 메틸화가 나타나지 않았다. 이로써 Bcl-2 유전자군과 암세포의 세포사멸을 억제하는 기작을 DNA의 메틸화를 통한 특이 유전자 발현 억제에 의해서 이루어질 수 있다고 사료된다. Objective: Promoter methylation of Bcl-2 family genes in cancer cells were studied to verify possible correlation between DNA methylation pattern of Bcl-2 family members and cancer. Methods: The genomic DNAs were extracted from different cancer cell lines, HeLa, CaSki and K562, and ovarian cancer tissue from patients. The cytosine residues were converted to uracil by sodium bisulfite treatment. MSP (methylation specific PCR) was performed to determine the methylation status of Bcl-2, Mcl-1, Noxa, and Harakiri promoters. Using primers that distinguish methylated DNA from unmethylated DNA after bisulfite modification of DNA, MSP was conducted to observe the methylation pattern of Bcl-2 family genes in different cancer cells. Results: The promoter regions of Bcl-2 family genes including Mcl-1, Bcl-2, and Noxa were not methylated in cancer cells, whereas the proapoptotic Bcl-2 family gene Harakiri was detected as methylated in the cancer cell lines and hypomethylated in the ovarian cancer tissue. Conclusion: The present study demonstrated the differential methylation profiles of Bcl-2 family genes in cancerous cells, which suggests a possible connection between the methylation pattern of some of Bcl-2 family genes and ovarian cancer.