Efficacy of Intracytoplasmic Sperm Injection for Leukocytospermia

강희규,--,--,--,--,--,--,--,-- THE KOREAN SOCIETY FOR BIOMEDICAL LABORATORY SCIEN 2004 Journal of biomedical laboratory sciences Vol.10 No.1

White blood cells (WBCs) are present in most human ejaculates, but abnormally high concentration of seminal leukocytes map reflect an underlying pathological condition. The World Health Organization (WHO) has defined leukocytospermia as status of more than 10^(6) WBC/mL of semen. The purpose of this study was firstly, to compare the outcomes between conventional IVF and ICSl in leukocytospermia, and secondly, to investigate whether ICSI may be an alternation treatment for patients with leukocytospermia. Total 346 cycles of conventional IVF and ICSI candidates underwent IVF cycles at Eulji Hospital Infertility Clinic. Semen Parameters including concentration, motility, morphology of spermatozoa and concentration of leukocytes were assessed from the raw ejaculates. There was no difference in sperm concentration, motility and morphology. The rates of fertilization and good embryo development from ICSI were significantly higher than those from conventional IVF in leukocytospermia (60.4% & 32.5% respectively for ICSI group and 44.4% & 28.5%, respectively for IVF group, P<0.001). The pregnancy rate after ICSI was also higher than that from conventional IVF (34.0% vs 29.1%. P<0.05). These results indicate that the presence of seminal leukocyes (>1×10^(6) WBC/mL of semen) is adversely related with fertilization, embryo development and pregnancy rate. Therfore the measurement of seminal leukocytes in routine semen analysis appears to be of prognostic value with regard to male fertilizing potential. In conclusion. it is suggested that ICSI is an alternative choice of treatment for patients with leukocytospermia.

시비방법별 벼 재배에 따른 전과정평가 방법을 적용한 환경영향 평가

신중두,임동규,김건엽,박문희,고문환,엄기철 한국환경농학회 2003 한국환경농학회지 Vol.22 No.1

Consoli가 설명한 것처럼 전과정평가의 개념 및 일반적인 정의에 따르면, 전과정평가는농업생산에 관련된 환경영향을 평가하는데 부합하는 방법이라고 생각되며, 특별히 전과정 평가방법인 Eco-indicator 95는 농업 체재에 대한 환경영향을 분석하는데 적절한 기법인 것으로 입정되었다. Eco-indicator 95 method를 이용하여 지구온난화 및 수계 부영양화와 관련된 시비체계에 따른 벼 재배에 대해 비교할만한 분석체계를 이루었다. 그렇지만 본 연구에서 전화정평가 항목에 기록된 모든 관련 정보가 환경 영향에 고려되지 않았기 때문에 Eco-indicator 95 method를 농업생산 체계에 적용할 때 몇 가지 난제에 부닥친다. Eco-indicator 95 method에 토양 및 자원의 이용과 같은 몇 몇 중요한 환경적인 문제가 포함되지 않았으며, 이 방법의 다른 문제는 환경 평가를 위하여 현장 정밀연구가 수행되지 않았다는 것이다. 그렇지만 본 연구에서 얻어진 생태지표 지수를 이용하여 벼 재배에 따른 시비방법 간의 차이점을 나타내기에 충분하다고 여겨지며, 가장 높은 생태지표 지수를 나타낸 화학비료를 시용한 구에서 가장 큰 환경영향이 관측되었고, 이러한 차이점은 주로 화학비료 투입에 의한 토양중의 높은 인산 함량의 축적 때문인 것으로 여겨진다. 분석한 시비체재는 특별히 지구온난화 보다는 수계의 부영양화의 환경적인 문제에 기여되는 것으로 나타났으므로 질소 시용비율 및 시용기술 이외에 액비 시용에 따른 양분 용탈이 수계 환경에 명확히 영향을 미치는 것으로 나타났다. The suitability of the Life Cycle Assessment (LCA) methodology to analyze'the environmental impact of rice cultivation with different fertilizing systems is investigated. The first part of an LCA is an inventory of parameters used and emissions released due to the system under investigation. In the following step, the Life Cycle Impact Assessment, the inventory data were analyzed and aggregated in order to finally get one index representing the total environmental burden. For the Life Cycle Impact Assessment (LCIA) the Eco-indicator 95 method has been chosen because this is well documented and regularly applied impact assessment method. The resulting index is called Ec -Indicator value. The higher the Eco-indicator value the stronger is the total environmental impact of an analyzed few system. The rice field experiment conducted in middle parts of korea was chosen as an example for the life cycle impact analysis. In this experiment the treatments were consisted of none fertilizer plot (NF), standard fertilizer plot (SF) applied chemical fertilizers based on soil chemical analysis before rice transplan ing, and efflux fertilized plot (EF) applied with pig wastes fermented as the same rates of SF plot as basis on total nitrogen content. The obtained Eco-indicator values were clearly different among the treatments in the rice trial. The total Eco-Indicator values for SF and EF have been observed 58 and 38% relative to the NF, respectively. For all the treatments the environmental effects of eutrophication contributed most to the total Eco-indicator value. The results appeared that the LCA methodology is basically suitable to assess the environmental impact associated with different fertilizer applications for rice cultivation. A comparative analysis of the fertilizing system's contribution to global warming and eutrophication is possible.

Hwanggeumchal sorghum extract enhances BMP7 and GH signaling through the activation of Jak2/STAT5B in MC3T3-E1 osteoblastic cells

JOUNG, YOUN HEE,LIM, EUN JOUNG,DARVIN, PRAMOD,JANG, JU WOONG,PARK, KYUNG DO,LEE, HAK KYO,KIM, HEUI SOO,CHO, BYUNG WOOK,PARK, TAEKYU,CHUNG, SUMI,PARK, JONG HWAN,YANG, YOUNG MOK Spandidos Publications 2013 MOLECULAR MEDICINE REPORTS Vol.8 No.3

N-glycoproteomic analysis of human follicular fluid during natural and stimulated cycles in patients undergoing in vitro fertilization

Lim, Hee-Joung,Seok, Ae Eun,Han, Jiyou,Lee, Jiyeong,Lee, Sungeun,Kang, Hee-Gyoo,Cha, Byung Heun,Yang, Yunseok The Korean Society for Reproductive Medicine 2017 Clinical and Experimental Reproductive Medicine Vol.44 No.2

Objective: Hyperstimulation methods are broadly used for in vitro fertilization (IVF) in patients with infertility; however, the side effects associated with these therapies, such as ovarian hyperstimulation syndrome (OHSS), have not been well studied. N-glycoproteomes are subproteomes used for the remote sensing of ovarian stimulation in follicular growth. Glycoproteomic variation in human follicular fluid (hFF) has not been evaluated. In this study, we aimed to identify and quantify the glycoproteomes and N-glycoproteins (N-GPs) in natural and stimulated hFF using label-free nano-liquid chromatography/electrospray ionization-quad time-of-flight mass spectrometry. Methods: For profiling of the total proteome and glycoproteome, pooled protein samples from natural and stimulated hFF samples were selectively isolated using hydrazide chemistry to obtain the total proteomes and glycoproteomes. N-GPs were validated by the consensus sequence N-X-S/T (92.2% specificity for the N-glycomotif at p<0.05). All data were compared between natural versus hyperstimulated hFF samples. Results: We detected 41 and 44 N-GPs in the natural and stimulated hFF samples, respectively. Importantly, we identified 11 N-GPs with greater than two-fold upregulation in stimulated hFF samples compared to natural hFF samples. We also validated the novel N-GPs thyroxine-binding globulin, vitamin D-binding protein, and complement proteins C3 and C9. Conclusion: We identified and classified N-GPs in hFF to improve our understanding of follicular physiology in patients requiring assisted reproduction. Our results provided important insights into the prevention of hyperstimulation side effects, such as OHSS.

Biochemical and morphological effects of hypoxic environment on human embryonic stem cells in long-term culture and differentiating embryoid bodies

Lim, Hee-Joung,Han, Jiyou,Woo, Dong-Hun,Kim, Sung-Eun,Kim, Suel-Kee,Kang, Hee-Gyoo,Kim, Jong-Hoon Springer-Verlag 2011 Molecules and cells Vol.31 No.2

Changes in Body Composition, Insulin, Glucose, Lipid Profiles and Insulin-Like Growth Factor 1 (IGF-1) in a Prader Willi Syndrome (PWS) Patient with Type 2 Diabetes During Long-Term Exercise : A Single Case Study

( Hee Joung Joung ),( Yong Ho Lee ),( In Su Lim ) 한국체육학회 2015 국제스포츠과학 학술대회 Vol.2015 No.1

Purpose: The Purpose of this study was to report changes in body composition, Insulin, Glucose, lipid profiles and IGF-1 in a Prader Willi syndrome (PWS) patient with type 2 diabetes during long-term exercise. In addition, this paper would like to propose the use of exercise as a basis treatment for PWS patients for not only relieving GH side effect but also improving obesity. Method: The subject was a 23-year-old man in a weight (107kg), height (151.1cm) and BMI (47.4kg/m²). He was diagnosed PWS and type 2 diabetes at age 12 years. He has taken medison and injection for type 2 diabetes. Exercise sessions were performed 5 times per week for 24 weeks. Intensity of exercise gradually increased, including the endurance and resistance exercise. Result: Body composition decreased in BMI to 44.4kg/m², weight to 101.3kg, fat mass to 51.1 % and lean mass to 29.6kg. Mean blood glucose concentration decreased from 123mg/dL to 109mg/dL, while insulin values increased from 2.3μU/mL to 9.9 μU/ml. At 15 and 20 week, he was prescribed to stop the insulin injection. Although lipid profiles increased, TG/HDL-C ratio decreased from 3 at risk level to 1.4 at normal level. Leptin level waxed and waned. IGF-1 levels increased from 90μg/L to 170μg/L. Conclusion: This case presents that long-term exercise could have beneficial effects on improvement of abnormal body composition, insulin sensitivity and IGF-1 in a PWS patient with type 2 diabetes. With these findings, although exercise in PWS patients cannot entirely substitute for a GH treatment, it could take the role of relieving side effects of GH treatment and improving obesity. We therefore conclude that long-term exercise in PWS patients with type 2 diabetes and GHD may not only improve risk factors related to obesity but also relieve GH side effects. Thus, we propose that exercise would be used as a basis treatment for PWS patients with or without GH treatment.. ……..

A proteomic study of the tissue repair induced by secretome released from human embryonic stem cell-derived hepatocytes in acute liver injuries

Hee-Joung Lim,Jiyou Han,Yu Jin Jang,Ae Eun Seok,Su Yoen An,Jong-Moon Park,Hoo Keun Lee,Hee-Gyoo Kang,Jong-Hoon Kim 한국발생생물학회 2013 한국발생생물학회 학술발표대회 Vol.2013 No.8

Hepatocytes derived from human embryonic stem cells (hESCs) may be a useful source for the treatment of diseased or injured liver. However, a low survival rate of grafted hepatocytes and immune rejection are still major obstacles to be overcome. We previously showed that secreted proteins (secretome) from hESC-derived hepatocytes had a potential therapeutic power in the tissue repair of injured liver without cell transplantation. The purpose of the present study was to discover key protein(s) in the secretome of hESC-derived hepatocytes using proteomic analysis and to study the tissue repair mechanism which may be operated by the secretomes. Purified indocyanine green+ hepatocytes derived from hESCs displayed multiple hepatic features, including expression of hepatic genes, production of albumin, and glycogen accumulation. The nano-LC/ESI-QTOF-MS analysis identified 365 proteins in the secretome of hESC-derived hepatocytes and the protein functional network analysis was conducted using the MetaCore TM from GeneGO. In addition, 20 tissue regeneration-related transcription factors (TFs) were extrapolated through further proteomic analysis. After intraperitoneal injection, the secretome significantly promoted the liver regeneration in a mouse model of acute liver injury. Protein functional network analysis on the secretome-induced regenerating liver confirmed 20 transcription factors (TFs) which were identified in the ICGhigh cells. The upreguation of these tissue repair-related TFs were validated by qPCR and western blotting on the regenerating liver tissues. These results demonstrate that application of the secretome analysis in combination with the protein functional network mapping would provide a reliable tool to discover new tissue-regenerating proteins as well as to expand our knowledge of the mechanisms of tissue regeneration.

Changes in body composition, blood lipid profile, and growth factor hormone in a patient with Prader- willi syndrome during 24 weeks of complex exercise: a single case study

( Hee Joung Joung ),( In Soo Lim ) 한국운동영양학회 2018 Physical Activity and Nutrition (Phys Act Nutr) Vol.22 No.1

[Purpose] Prader-Willi syndrome (PWS) is a genetic disorder characterized by excessive appetite with progressive obesity and growth hormone (GH) deficiency. Excessive eating causes progressive obesity with increased risk of morbidities and mortality. Although GH treatment has beneficial effects on patients with PWS, adverse events have occurred during GH treatment. Exercise potentially has a positive effect on obesity management. The purpose of this research was to examine the effects of 24-week complex exercise program on changes in body composition, blood lipid profiles, and growth factor hormone levels in a patient with PWS. [Methods] The case study participant was a 23-year-old man with PWS who also had type II diabetes mellitus because of extreme obesity. Complex exercises, including strength and aerobic exercises, were conducted 5 times one week for 60 minutes per session, over 24 weeks. Blood sampling was conducted five times: before and at 8, 16, 20, and 24 weeks after commencement of the exercise program. [Results] Weight, fat mass, triglycerides/ high-density lipoprotein (TG/HDL) ratio, mean blood glucose, and GH decreased after training. Blood insulin and insulin-like growth factor (IGF-1) levels increased after training. At 15 and 20 weeks, insulin injection was discontinued. Insulin levels increased and average blood glucose decreased to normal levels; IGF-1 increased continuously during the 24- week exercise program. [Conclusion] Twenty-four weeks of complex exercises had a positive effect on obesity and diabetes in the patient with PWS. Therefore, long-period complex exercises might be an effective intervention for improvement of metabolic factors in PWS patients.

Biochemical and Morphological Effects of Hypoxic Environment on Human Embryonic Stem Cells in Long-Term Culture and Differentiating Embryoid Bodies

Hee-Joung Lim,한지유,Dong-Hun Woo,Sung-Eun Kim,김슬기,강희규,김종훈 한국분자세포생물학회 2011 Molecules and cells Vol.31 No.2

The mammalian reproductive tract is known to contain 1.5-5.3% oxygen (O_2), but human embryonic stem cells (hESCs) derived from preimplantation embryos are typically cultured under 21% O_2 tension. The aim of this study was to investigate the effects of O_2 tension on the long-term culture of hESCs and on cell-fate determination during early differentiation. hESCs and embryoid bodies (EBs) were grown under different O2 tensions (3, 12, and 21% O_2). The expression of markers associated with pluripotency, embryonic germ layers, and hypoxia was analyzed using RT-PCR, immunostaining, and Western blotting. Proliferation, apoptosis, and chromosomal aberrations were examined using BrdU incorporation, caspase-3 immunostaining, and karyotype analysis, respectively. Structural and morphological changes of EBs under different O_2 tensions were comparatively examined using azan- and hematoxylin-eosin staining, and scanning and transmission electron microscopy. Mild hypoxia (12% O_2) increased the number of cells expressing Oct4/Nanog and reduced BrdU incorporation and aneuploidy. The percentage of cells positive for active caspase-3, which was high during normoxia (21% O_2), gradually decreased when hESCs were continuously cultured under mild hypoxia. EBs subjected to hypoxia (3% O_2) exhibited well-differentiated microvilli on their surface, secreted high levels of collagen, and showed enhanced differentiation into primitive endoderm. These changes were associated with increased expression of Foxa2, Sox17, AFP, and GATA4 on the EB periphery. Our data suggest that mild hypoxia facilitates the slow mitotic division of hESCs in long-term culture and reduces the frequency of chromosomal abnormalities and apoptosis. In addition, hypoxia promotes the differentiation of EBs into extraembryonic endoderm.

Poster Session : Cancer Biology ; Hypoxia up-regulates the insulin-like growth factor-1 expression via STAT5b pathway in human hepatocarcinoma cells

( Youn Hee Joung ),( Eun Joung Lim ),( Mi Sun Kim ),( Ill Min Chung ),( Young Mok Yang ) 한국생화학분자생물학회 (구 한국생화학회) 2007 생화학분자생물학회 춘계학술발표논문집 Vol.2007 No.-