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이영자,김소희,김진숙,한정아,서해점,임효정,최수영,Lee Young Ja,Kim So Hee,Kim Jin-Sook,Han Jeong A,Seo Hae Jeom,Lim Hyo Jeong,Choi Soo Young The Korean Society of Food Hygiene and Safety 2005 한국식품위생안전성학회지 Vol.20 No.3
역상 컬럼을 이용하여 건강기능식품 중 클로렐라 및 스피루리나제품에 함유되어 있는 엽록소 a, b,페오포르바이드 a 및 $\beta$-카로틴의 HPLC동시분석법을 확립하였으며, 첨가농도 $50\;\mug/ml$에서 엽록소 a, b, 페오포르바이드 a 및 $\beta$-카로틴에 대한 회수율시험결과, 각각 2.8, 6.0, 10.6 및 $10.4\%$의 상대표준편차와 70.3, 71.6, 60.1 및 $90.5\%$의 회수율을 각각 나타냈다. 이때 검출한계는 $0.1\sim1.0\;\mug/ml$, 정량한계는 $0.2\sim2.0\;\mug/ml$이었으며 검량선 상관계수도 0.995 이상의 직선성을 보여주었다. 국내유통 클로렐라 및 스피루리나제품에 대한 엽록소 a, b,페오포르바이드 a및 $\beta$-카로틴의 함유량을 분석한 결과 엽록소 a $121.g\sim543$, 엽록소 b $0.6\sim160.0$, 페오포르바이드 a 및 P-카로틴 $383.6\sim1713.7mg/ml$ 수준으로 나타났다. 엽록소 b의 함유량은 클로렐라제품에서 평균 374.0 mg/100 g 으로 스피루리나제품의 평균 10.5 mg/100 g 보다 30배 이상 함유하고 있는 것으로 확인되었다. 그러나 $\beta$-카로틴의 함유량은 스피루리나제품이 평균 1335.4 mg/100 g 로 클로렐라제품의 평균 495.0 mg/100 g 보다 평균 함유량에서 2.7배 높은 것으로 나타났다. 국내 건강기능식품공전 중 클로렐라 및 스피루리나제품의 엽록소 a b, 및 페오포르바이드 항목의 규격검사를 본 연구의 동시분석법으로 개정함으로써 각 성분 함량의 정량, 분석시간의 단축 및 비용절감 둥 시험방법을 크게 개선할 수 있을 것으로 기대된다. A simple and sensitive analysis method based on reverse phase (RP) HPLC with UV detector was developed for simultaneous determination of chlorophyll a and b, pheophorbide a and $\beta-Carotene$ in Chlorella and Spirulina products. For added concentration $(50\;\mug/ml)$ of chlorophyll a and b, pheophorbide a and $\beta-Carotene$, recoveries of those were 70.3, 71.6, 60.1 and $90.5\%$, respectively, with relative standard deviations of 2.8,6.0, 10.6 and $10.4\%$. Limit of detection and quantification had ranges of $0.1\sim1.0\;\mug/ml$ and $0.2\sim2.0\;\mug/ml$, respectively. Calibration curve was linear with correlation coefficient of 0.995 for chlorophyll a and b, pheophorbide a and $\beta-Carotene$. Results of simultaneous determination in Chlorella and Spirulina products were showed ranges of $121.g\sim543.0\;\mug/ml$ for chlorophyll a,$0.6\sim160.0\;\mug/ml$ for chlorophyll b, $19.2\sim60.3\;\mug/ml$ for pheophorbide a and $383.6\sim1713.7\;\mug/ml$ for $\beta-Carotene$, respectively. Chlorophyll b contents in Chlorella products were detected above 30 times level to those in Spirulina products. $\beta-Carotene$ contents in Spirulina products were detected 2.7 times level to those in Chlorella products.
Asobara japonica의 벗초파리 발육단계에 따른 기생특성 및 살충효과
윤승환,이진구,장재은,한정아,김상우,김아영,서홍렬,이영순,Yun, Seung-Hwan,Lee, Jingu,Jang, Jae Eun,Han, Jeong-A,Kim, Sang-Woo,Kim, A-Young,Seo, Hong-Yul,Lee, Young Soon 한국응용곤충학회 2021 한국응용곤충학회지 Vol.60 No.3
벗초파리기생벌인 A. japonica의 벗초파리 유충의 발육단계에 따른 기생특성과 기생당한 유충과 번데기에서 형태적인 차이를 조사하였다. 또한 A. japonica의 우화기간이 벗초파리 우화일수 보다 더 소요되는 것을 확인하였다. A. japonica는 벗초파리 유충에 효과적인 기생 및 살충 효과를 나타내었으며 벗초파리 방제를 위한 천적으로 활용이 가능할 것으로 보인다. We confirmed the parasitic characteristics of Asobara japonica according to the stage of development of Drosophila suzukii and observed the morphological differences between parasitized and unparasitized D. suzukii larvae and pupae. It was also confirmed that emergence period of A. japonica was longer than that of D. suzukii. A. japonica showed an effective parasitic and insecticidal effect on D. suzukii larvae, and it is expected that A. japonica could be used as a natural enemy for the control of D. suzukii.
이은섭(Eun Seob Yi),김진영(Jin Young Kim),이종형(Jong Hyong Lee),이진구(Jin Goo Lee),한정아(Jeong A Han),강창성(Chang Seong Kang) 한국육종학회 2018 한국육종학회지 Vol.50 No.3
The soybean cultivar ‘Gangpoong’ was developed for soy-paste and tofu. Suwon 223 and SS 00419 were crossed in 1999 and their progenies were selected from F3 to F5 using the pedigree method. Preliminary yield (PYT) and advanced yield (AYT) trials were conducted from 2008 to 2010, and regional yield trials (RYT) were conducted at three locations from 2011 to 2013. In RYT, ‘Gangpoong’ was stable in variable environments. ‘Gangpoong’ was determinate, with white flowers, yellow elongated spherical seeds, and a light brown hilum. The flowering and maturity dates were July 27 and October 8, respectively. The plant height was 67 cm shorter than that of ‘Daewonkong’ (81 cm, standard cultivar). ‘Gangpoong’ had fewer node numbers (16) than that of ‘Daewonkong (18) and had a heaver seed weight (30.1 g/100-seed weight) than that of ‘Daewonkong (26.0 g/100-seed weight)’. ‘Gangpoong’ showed lodging tolerance. The tofu yield of ‘Gangpoong’ was 213%, and the physical characteristics of tofu were similar to those of ‘Daewonkong (208%, standard cultivar)’. The soybean malt scent and fermented soybean yield of ‘Gangpoong’ were 2 and 105%, respectively. The yield in adaptable regions was 3.4 MT/ha, which was 14% higher than that of ‘Daewonkong’. Therefore, ‘Gangpoong’ could be cultivated and used widely for soy-paste and tofu in the near future (Registration number: 5932).
한정아,김종태,Han, Jeong-A,Kim, Jong-Tae 한국광학회 2007 한국광학회지 Vol.18 No.2
A high power LED which is being used in many illumination applications as a new light source was simulated for its physical structure and then its optical properties were analyzed. To obtain accurate results from the designed LED model, properties of the die chip and reflector cup were varied. As a result, a high power LED model which has a radiation pattern of a Lambertian with its viewing angle of approximately $140^{\circ}$ and total included angle of $160^{\circ}$ was designed. 새로운 광원으로 각종 조명장치에 응용이 되는 고출력 LED 광원의 물리적 구조로부터 시뮬레이션을 통하여 광학적 특성을 분석하였다. 설계한 LED 광원이 신뢰할 수 있는 결과를 얻기 위하여 die chip과 reflector cup의 구조적 특성을 변화시켰으며. 그 결과 Lambertian 형태의 배광 분포를 가지며. 반치 폭(Viewing angle)은 $140^{\circ}$, Total Included Angle은 $160^{\circ}$를 갖는 고출력 LED 광원을 설계하였다.
한정아(Jeong A Han),추지은(Ji Eun Choo),손지원(Jee Won Shon),김윤숙(Youn Sook Kim),서수연(Su Yeon Suh),안원근(Won Gun An) 한국생명과학회 2019 생명과학회지 Vol.29 No.2
시스템 약리학적 분석을 통해 대회향(Anisi Stellati Fructus)의 활성성분 스크리닝, 표적유전자 확보 및 관련 질병과의 네트워크를 구축한 후 대회향의 항균작용을 중점적으로 분석하였다. Traditional Chinese Medicine Systems Pharmacology (TCMSP) Database 와 Analysis Platform을 통해 대회향의 잠재적 활성성분 49개를 확보하였으며, 그 중 설정한 조건에 부합하는 9개 활성성분을 스크리닝 하였다. TCMSP Database는 활성성분의 약물 동태학적 특성 및 약물-표적-질병 간의 관련성을 네트워크 수준에서 파악할 수 있는 획기적인 in silico적 접근을 가능하게 해준다. 활성성분과 반응하는 201개의 유전자 정보를 UniProt database를 통해 확인하고, 취합한 유전자들이 관여하는 348개의 생물학적 과정을 David 6.8 Gene Functional Classification Tool에서 확보하였다. Chemokine ligand 2, Interleukin-10, Interleukin-6, Tumor Necrosis Factor를 포함한 총 47개의 유전자가 항균작용에 관여하였고 이들을 표적으로 하는 luteolin, kaempferol, quercetin 등이 대표적 항균 관련 활성성분이었다. 이와 같이 확보된 데이터는 연구 재료 선정에 정확성과 시간, 노력, 비용 절감의 효과를 제공함과 더불어 추후 실험적 증명으로 이어져 감염병의 예방과 치료 전략에 과학적인 근거를 제시할 수 있을 것이다. The purpose of this study was to acquire the active compounds of Anisi stellati fructus (ASF) and to analyze the genes and diseases it targets, focusing on its antibacterial effects using a system pharmacological analysis approach. Active compounds of ASF were obtained through the Traditional Chinese Medicine Systems Pharmacology (TCMSP) Database and Analysis Platform. This contains the pharmacokinetic properties of active compounds and related drug–target–disease networks, which is a breakthrough in silico approach possible at the network level. Gene information of targets was gathered from the UnitProt Database, and gene ontology analysis was performed using the David 6.8 Gene Functional Classification Tool. A total of 201 target genes were collected, which corresponded to the nine screened active compounds, and 47 genes were found to act on biological processes related to antimicrobial activity. The representative active compounds involved in antibacterial action were luteolin, kaempferol, and quercetin. Among their targets, Chemokine ligand2, Interleukin-10, Interleukin-6, and Tumor Necrosis Factor were associated with more than three antimicrobial biological processes. This study has provided accurate evidence while saving time and effort to select future laboratory research materials. The data obtained has provided important data for infection prevention and treatment strategies.