불소 함유 양성고분자의 분리와 특성

차미선,소원욱,박인준,이수복 한국공업화학회 2004 응용화학 Vol.8 No.1

We describe the synthesis and characterization of amphoteric poly(acrylate), consisting of the F-marcromer and a 2-(dimethyl amino)ethyl methacrylate (2-DMAEMS) The amphoteric polymer were prepared by marcromonomer technique and radical copolymerization. After copolymerization, the resultant rection solution was purified by silica column. In this study, separation of resultant reaction sloution was purified by silica column. In this study, separation of amphoteric poly(acrylate)s and characterization by InfraRed Spectroscopy, Gel Permeation Charomatography. The IR traces showed that the amphoteric poly(acrylate) was separated successfully polymr of p(2-DMAEMA). The amphoteric poly(acrylate) have number average molecular weights(Mw) about 38,000 by GPC.

불소함유 그라프트 공중합체의 표면특성

차미선,소원욱,박인준,김동권,이수복,ShiYuan, Cheng 한국공업화학회 2003 응용화학 Vol.7 No.2

The graft copolymer, consisting of a flurorinated macromer(F-macromer) and a 2-(dimethyl amino)ethyl methacrylate, were prepared by marcromonomer technique and radical copolymerization. In this study, the surface propeties of the graft copolymer and its additive effect on the surface energy of polymer film were compared with the random copolymer of poly(perfluoroalkyl ethyl acrylate)-r-poly(2-dimethylamino ethyl methacrylate)(PFA-r-P(2-DMAEMA)). The contact angles of the surface modified by the graft copolymer is large than that of random copolymer with similar FA monomer content, and surface free energy of the substrate containing 0.01wt% of the graft copolymer is similar with that of 0.1wt% random copolymer surface. That is, The low-energy modification effect of the graft copolymer is better than that of the random copolymer.

원유의 미생물적 유화

차미선,손홍주,이건,고명선,이상준,이종근 부산대학교 환경문제연구소 1996 環境硏究報 Vol.14 No.1

Microorganisms producing bioemulsifier were isolated from the sea water in Pusan costal aera. The isolated strain which had the highest emulsification activity and stability was identified as the genus Acinetobacter from the results of morphological, cultural and biochemical tests and named Acinetobacter sp. EL-C6 for convinience. The compositions of optimum medium for emulsification of crude oil by Acinetobacter sp. EL-C6 were crude oil 2%, NH_4NO_3 0.2%, K_2HPO_4 0.01%, MgSO_4·7H_2O 1%, CaCl_2·2H_2O 0.1%, NaCl 3% and the initial pH 7.5. The cultivation for emulsification of crude oil was carried out in 500m1 shaking flask containing 100m1 of the optimum medium at 30℃. The highest emulsification was observed after 5 days. The utilization on the various hydrocarbon of the Acinetobacter sp. EL-C6 was showed that the utilization of n-alkane compounds were better than that of aromatic compounds. Among the petroleum compounds, by Acinetobacter sp. EL-06.

서울시내 국민학교의 비만아동에 관한 연구

차미선 한국초등교육학회 1988 초등교육연구 Vol.2 No.1

The purpose of the study is to find out factors which are related to children's obesity. The subjects of the study are mothers of 790 5th and 6th obesiey and normal children from elementary school in Seoul, Korea. The study was conducted in March and April, 1985 by the method of questionaire. The results of the study are as follow : 1. Mothers have misunderstanding about physical health of child. a. Mothers think that obese child is healthy child. b. Mothers provide children food without limits as an expression of affection. 2. Obese children have tendency for overeat compare to normal childrens. a. The foods which children generally like are rice, instant noodles, ice-cream, and soft drinks. b. The obese children eat more grain (rice and instant noodles) compare to normal children. 3. Generally children are lack of physical exercise. Mothers have tendency to limit their children to play at home because of danger of acaidence, and lack of safe playgrounds.

수용성 금속가공유 폐액의 생물학적 처리

차미선,한창민,박근태,조순자,손홍주,이상준 한국생명과학회 2003 생명과학회지 Vol.13 No.6

P. aeruginosa EMS1에 의한 수용성 절삭유의 생물학적 처리를 위한 환경요인과 최적조건을 검토한 결과는 다음과 같다. 절삭유속에 함유된 각종 성분을 이용한 P aeruginosa EMSl의 생육 및 절삭유 분해 가능성을 검토하기 위하여 증류수에 절삭유를 1% 농도로 첨가하여 멸균한 후, P aeruginosa EMSl를 접종하여 균체 생육도 및 COD 제거율을 조사한 결과, 균주의 생육도는 극히 미약하고 약 12%의 COD 제거능을 가지고 있었다. 이것은 균주의 생육과 효율적인 COD제거능을 위하여 절삭유 속에 함유된 성분 외의 다른 일반적인 배지성분이 필요함을 시사한다. P. aeruginosa EMS1에 의한 1% 수용성 절삭유의 COD 제거율과 균주 생육도 향상을 위한 최적 배지조성은 0.3% NH_4NO_3,\; 0.05%\; K_2HPO_4,\; 0.04% KH_2PO_4,\; 0.05%\; MgSO_4.7H_2O,\; 0.03%\; CaCl_2.2H_2O,\; 0.04%\; FeSO_4.7H_2O$이었고, 최적배양 조건은 pH 7,$30^{\circ}C$, 50m$\ell$ / 300m$\ell$flask이었다. 이 조건에서 배양 4일 후, 87%의 COD가 제거되었다. 최적배지에서 COD 제거에 미치는 절삭유 농도의 영향을 조사한 결과, 절삭유 농도가 낮을수록 relative COD(%)가 낮았으며, 균체 생육도가 높음을 알 수 있었다. 따라서 본 연구의 결과를 바탕으로 하여 현재 폐절삭유의 처리를 위해 시행되고 있는 물리화학적 처리 시스템의 후속 공정으로 생물학적 처리시스템을 구축하고, 표준 균주로서 P. aeruginesa EMS1이 사용될 경우, 잔여 절삭유의 완전 분해가 가능할 것으로 사료된다. The present investigation was conducted to determine the chemical oxygen demand (COD) removal efficiency by Pseudomonas aeruginosa EMS1. The COD removal efficiency in the medium containing 1% metal working fluid (MWF) was 12% after cultivation of 4 days. The composition of optimum medium for the COD removal efficiency of 1% MWF by P. aeruginosa EMS1 were NH$_4$Cl 0.3%,$ K_2HPO_4\; 0.05%,\; KH_2PO_4\; 0.04%,\; MgSO_4.7H_2O\; 0.05%,\; CaCl_2.2H_2O 0.03%$ and $FeSO_4.7H_2O$ 0.04% at initial pH 7.0 and $30^{\circ}C$. Under this condition, the highest the COD removal efficiency was observed after 4 days.

Positive Effects of Bisphosphonates on Osteogenic Differentiation in Patient-Derived Mesenchymal Stem Cells for the Treatment of Osteoporosis

차미선,이경미,이재협 한국조직공학과 재생의학회 2018 조직공학과 재생의학 Vol.15 No.4

BACKGROUND: Recent evidence from in vitro and in vivo studies indicates that bisphosphonates may promote osteoblastic bone formation and potently inhibit osteoclast activity. However, little is known about the potential effect of bisphosphonates on the recruitment of osteoblastic precursors from patient-derived bone marrow stromal cells due to difficulties in accessing human bone marrow from healthy and disease subjects. METHODS: In this study, we evaluated the potential of using FDA-approved and clinically utilized bisphosphonates such as alendronate, ibandronate, and zoledronate to enhance the development of bone forming osteoblasts from osteoporosis patient- and healthy-person derived hBMSCs (op-MSCs and hp-MSCs, respectively). hBMSCs were obtained from postmenopausal women without endocrine diseases or receiving hormone replacement therapy. Cells were treated with or without a bisphosphonate (alendronate, ibandronate, and zoledronate) and analyzed over 21 days of culture. RESULTS: hBMSC from osteoporosis-patient with bisphosphonates treatment demonstrated a significant increase in Alizarin red staining after 7 days compared to that from healthy-person. Calcium contents and alkaline phosphatase (ALP) enzyme activity also demonstrated an increased propensity in hMSCs from osteoporosis-patient compared to those from healthy-person, although there were inter-individual variations.Gene expression levels varied among different donors. There were no significant differences in the effect on the osteoblastic differentiation of hBMSCs among alendronate, ibandronate, and zoledronate. Statistical significance in the osteoblastic differentiation of hBMSCs between the positive control group cultured in osteogenic mediumalone and groups cultured in osteogenic mediumsupplemented with bisphosphonate was not shown either.These results might be due to various cell types of hBMSCs from individual clinical patients and concentrations of bisphosphonate used. CONCLUSION: Our study using a clinically relevant in vitro model suggests that bisphosphonate treatment is more effective for patients with osteoporosis than its preventive effect for healthy person. In addition, patient-specific responses to bisphosphonates should be considered rather than bisphosphonate type prior to prescription. Further investigations are needed to determine how bisphosphonates influence hBMSCs function to mediate bone quality and turnover in osteoporotic patients. Such studies can generate novel approaches to treat age-related osteoporotic bone loss.

기재 및 Hardcoat제에 따른 광경화 오염방지제의 코팅표면특성 관찰

차미선,김세미,주민혁,정종국 한국공업화학회 2015 한국공업화학회 연구논문 초록집 Vol.2015 No.0

본 실험에 사용된 광경화 오염방지제는 HFPO(Hexafluoropropylene oxide) 음이온 중합에 의해 합성된 말단 관능기를 갖는 PFPE(Perfluoropolyether)를 원료로 사용하였으며, 헥사메틸렌 디이소시아네이트 사이클릭트라이머 및 광반응성기를 갖는 단량체를 반응시켜, 과불소 폴리에테르 변성화합물을 합성하였다. 합성된 변성화합물과 상용적으로 사용하고 있는 hardcoat제를 혼합하여 다양한 기재 표면에 코팅하여 표면특성을 관찰하였다. 사용된 기재는 PET, PMMA, slideglass를 사용하였으며, 접촉각, 코팅경도, 지우개 내마모성 등을 측정하였다. 코팅상태 및 표면특성은 hardcoat제와의 상용성에 크게 영향을 받는 것으로 나타났다.

국내 박물관 보존환경에서의 세균 분포 실태조사

차미선,윤수정,이나은,권영숙,이상준 부산대학교 문화유산보존연구소 2005 한국전통복식연구소 학술심포지움 Vol.- No.-

Three-dimensional printed polylactic acid scaffold integrated with BMP-2 laden hydrogel for precise bone regeneration

차미선,Yuan Zhe Jin,Jin Wook Park,Kyung Mee Lee,Shi Huan Han,Byung Sun Choi,Jae Hyup Lee 한국생체재료학회 2021 생체재료학회지 Vol.25 No.4

Background: Critical bone defects remain challenges for clinicians, which cannot heal spontaneously and require medical intervention. Following the development of three-dimensional (3D) printing technology is widely used in bone tissue engineering for its outstanding customizability. The 3D printed scaffolds were usually accompanied with growth factors, such as bone morphometric protein 2 (BMP-2), whose effects have been widely investigated on bone regeneration. We previously fabricated and investigated the effect of a polylactic acid (PLA) cage/Biogel scaffold as a carrier of BMP-2. In this study, we furtherly investigated the effect of another shape of PLA cage/Biogel scaffold as a carrier of BMP-2 in a rat calvaria defect model and an ectopic ossification (EO) model. Method: The PLA scaffold was printed with a basic commercial 3D printer, and the PLA scaffold was combined with gelatin and alginate-based Biogel and BMP-2 to induce bone regeneration. The experimental groups were divided into PLA scaffold, PLA scaffold with Biogel, PLA scaffold filled with BMP-2, and PLA scaffold with Biogel and BMP-2 and were tested both in vitro and in vivo. One-way ANOVA with Bonferroni post-hoc analysis was used to determine whether statistically significant difference exists between groups. Result: The in vitro results showed the cage/Biogel scaffold released BMP-2 with an initial burst release and followed by a sustained slow-release pattern. The released BMP-2 maintained its osteoinductivity for at least 14 days. The in vivo results showed the cage/Biogel/BMP-2 group had the highest bone regeneration in the rat calvarial defect model and EO model. Especially, the bone regenerated more regularly in the EO model at the implanted sites, which indicated the cage/Biogel had an outstanding ability to control the shape of regenerated bone. Conclusion: In conclusion, the 3D printed PLA cage/Biogel scaffold system was proved to be a proper carrier for BMP2 that induced significant bone regeneration and induced bone formation following the designed shape.

PFPE 합성 및 정제

차미선,김세미,주민혁,정종국 한국공업화학회 2014 한국공업화학회 연구논문 초록집 Vol.2014 No.1

PFPE(Perfluoropolyether)는 slip성 및 광학적 특성이 우수한 소재로 다양한 산업용도로 사용되고 있다. PFPE 합성물은 반응모노머로 HFPO(Hexafluoropropylene oxide), 촉매로 CsF(Caesium fluoride), 용매인 TG(Tetraethylene glycol dimethyl ether)를 사용하여 합성할 수 있다. 본 연구에서는 HFPO의 투입속도와 반응온도조건을 변화시켜 다양한 분자량 분포를 갖는 PFPE를 합성하였으며, 합성여부는 GC(Gas chromatography), GPC(Gel permeation chromatography), NMR(Nuclear magnetic resonance), IR(Infrared spectroscopy) 분석 등을 통해 확인하였다. 이렇게 합성된 PFPE-COF의 COF 작용기를 에스테르화 반응, 알코올 반응하여 광경화형 지문방지제의 원료가 되는 PFPE-OH를 합성하였다. 그러나 에스테르화 반응 시 반응 용매 중 포함된 수분 등의 영향으로 산(-COOH)등의 불순물이 일부 발생하면서 최종 생성물의 반응성, 표면특성 등에 영향을 주는 것으로 나타났다. 따라서 합성 중 생성되는 이러한 불순물을 제거하기 위해 흡착제를 사용하였으며, 제거여부는 GC분석을 통해 확인하였다.