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조승학 신학지남사 1931 신학지남 Vol.13 No.6

Possibility of CTX-M-14 Gene Transfer from Shigella sonnei to a Commensal Escherichia coli Strain of the Gastroenteritis Microbiome

조승학,한순영,강연호 질병관리본부 2014 Osong Public Health and Research Persptectives Vol.5 No.3

Objectives: To investigated whether the CTX-M-14 gene could be transferred from a clinical Shigella sonnei strain to commensal Escherichia coli strain in the gastroenteritis microbiome. Methods: E. coli strains were isolated from 30 stool samples of S. sonnei infected students in a gastroenteritis outbreak in 2004 and were characterized by antibiotic resistance analysis, in vitro conjugation and in vivo transfer of CTX-M-14 gene and molecular assays. Results: One strain of Escherichia coli that had high levels of resistance to cefotaxime was isolated from a patient infected with S. sonnei. Isoelectric focusing showed that the E. coli and S. sonnei strains produced a b-lactamase with an isoelectric point of 8.1. Moreover, polymerase chain reaction analysis indicated that both strains possessed the same DNA sequences for CTX-M-14. The results of in vitro and in vivo conjugation showed that the efficiency of CTX-M-14 transfer from S. sonnei to E. coli was similar to CTX-M-14 transfer between E. coli strains. Conclusion: The data suggest that the acquisition of the extended-spectrum blactamases gene by pathogenic bacteria in the human intestinal tract to commensal microbiome bacteria can cause serious infectious diseases.

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조승학 신학지남사 1930 신학지남 Vol.12 No.2

A Case of a Shiga Toxin Producing Escherichia Coli

조승학,이혜경,김중범,박용배,박미선,채현석 연세대학교의과대학 2011 Yonsei medical journal Vol.52 No.6

We encountered a patient with hemolytic uremic syndrome (HUS) with persistent isolation of shiga toxin-producing Escherichia coli (STEC) for 3 weeks despite of having no clinical symptoms. STEC has been recognized as an important food-borne pathogen that causes severe diseases such as HUS. We characterized this STEC strain via a polymerase chain reaction, reverse-passive latex agglutination and the slide agglutination method. In this STEC strain, stx2 (shiga toxin), eaeA, tir, iha (adherence genes), espADB (type III secretion genes), and hlyA, ehxA, clyA (hemolysin genes) were present. The O antigen of the strain was non-typable.

Preliminary study of Korean orthodontic residents’ current concepts and knowledge of cleft lip and palate management

조일식,신효근,백승학 대한치과교정학회 2012 대한치과교정학회지 Vol.42 No.3

Objective: A national survey was conducted to assess orthodontic residents’current concepts and knowledge of cleft lip and palate (CLP) management in Korea. Methods: A questionnaire consisting of 7 categories and 36 question items was distributed to 16 senior chief residents of orthodontic department at 11 dental university hospitals and 5 medical university hospitals in Korea. All respondents completed the questionnaires and returned them. Results: All of the respondents reported that they belonged to an interdisciplinary team. Nineteen percent indicated that they use presurgical infant orthopedic (PSIO)appliances. The percentage of respondents who reported they were ‘unsure’about the methods about for cleft repair operation method was relatively high. Eighty-six percent reported that the orthodontic treatment was started at the deciduous or mixed dentition. Various answers were given regarding the amount of maxillary expansion for alveolar bone graft and the estimates of spontaneous or forced eruption of the upper canine. Sixty-seven percent reported use of a rapid maxillary expansion appliance as an anchorage device for maxillary protraction with a facemask. Th ere was consensus among respondents regarding daily wearing time, duration of treatment, and amount of orthopedic force. Various estimates were given for the relapse percentage after maxillary advancement distraction osteogenesis (MADO). Most respondents did not have suffi cient experience with MADO. Conclusions: Th ese fi ndings suggest that education about the concepts and methods of PSIO and surgical repair, consensus regarding orthodontic management protocols, and additional MADO experience are needed in order to improve the quality of CLP management in Korean orthodontic residents.

구순구개열 및 두개안면기형 환자의 진료 및 교육현황에 관하여 치과교정과 전공의를 대상으로 한 기초설문 조사 결과

서유진,조일식,백승학 대한구순구개열학회 2012 대한구순구개열학회지 Vol.15 No.1

A national wide survey was conducted to assess a present condition in management of cleft and craniofacial anomaly patients and training program of orthodontic residents in Korea. A questionnaire consisting of four categories and 19 question items was distributed to 131 residents of department of orthodontics of eleven dental university hospitals and nine medical university hospitals. The results were as follows:(1) 77.1% of residents are participating in treatment of cleft and craniofacial anomaly patients.(2) Only 47.3% of residents are willing to treat cleft and craniofacial anomaly patients in their future practice.(3) 64.9% of residents responded that they are currently treating one to ten cleft and craniofacial anomaly patients per resident.(4) Most university hospitals offer training programs focusing on embryopathogenesis, growth, and treatment, but training programs about speech and hearing, genetics, and psychosocial development are inadequate.(5) 37.4% of residents are willing to participate in fellowship program for cleft and craniofacial anomaly after finishing the training. Based on the results of this survey, the residents need motivation regarding treatment of cleft and craniofacial anomaly patients, and the educational programs need to be reinforced and reconstructed so that standardization among hospitals can be achieved.

Proposal of Statistical Sampling Plans for Bacillus cereus in Korean Fermented Soybean Pastes

김중범,김철우,조승학,노완섭,김왕준 한국식품과학회 2015 Food Science and Biotechnology Vol.24 No.2

Bacillus cereus contamination is one of the major problems for food safety in Korean fermented soybean pastes. A one-sample inspection is not sufficient to determine the overall level of contamination. The aims of this study were to propose new statistical sampling plans for B. cereus in Korean fermented soybean pastes. The acceptable probability over 95% is considered suitable for sampling plan. We suggest statistical sampling plans for B. cereus A (n=5, c=2, m=3, and M=4), B (n=5, c=0, and m=4), or C (n=5, c=1, m=4, and M=5) in Korean fermented soybean pastes based on the B. cereus standard in current Korean Food Code and the median and mean value from the 150 pastes estimated in this study. The statistical sampling plans for B. cereus proposed in this study will provide an advanced food regulation and permit harmonization with international food standards.

한국인 하악전돌증환자의 유전적 영향과 유전율에 대한 분석

김영호(Young Ho Kim),조한영(Han Young Cho),백채환(Chae Hwan Baek),이아영(Ah-Young Lee),김군종(Gunjong Kim),김휘영(Whi Young Kim),서영주(Young Ju Suh),백승학(Seung-Hak Baek),홍종락(Jongrak Hong),팽준영(Jun-Young Paeng) 대한구강악안면외과학회 2010 대한구강악안면외과학회지 Vol.36 No.6

Introduction: This study examined the genetic influence of mandibular prognathism epidemiologically in Korean families. Materials and Methods: Over a 5-year period from 2005 to 2009, a questionnaire with a pedigree chart was given to 100 (male 51, female 49) probands with skeletal Class III mandibular prognathism, who had undergone orthognathic surgery in Samsung Medical Center. Results: The average age of the probands was 22.1. The average SNA, SNB and ANB angles of the probands were 81.2􀆆, 84.1􀆆and -2.9􀆆, respectively. A total of 2729 (male 1,354, female 1,375) family members were examined, and the affected ratio of the families was 3.5% with no significant difference between genders. 45% of families had at least one member with a Class III malocclusion other than the proband. The affected ratio of the first-degree relatives (10.9%) was significantly higher than those of the second-degree (3.3%) and third-degree (1.9%) relatives. The affected ratio of the total relatives from the male probands (4.2%) was significantly higher than that of the female probands (2.8%). Heritability (h2, Falconer’method) was estimated to be 29.8% (0.298±0.059) in first-degree relatives. Conclusion: These results showed the significant influence of mandibular prognathism with relatively low heritability in first-degree relatives in Korean families of probands, who had undergone orthognathic surgery to correct a skeletal Class III malocclusion.

Molecular characterization and antibiotic resistance of Staphylococcus aureus strains isolated from patients with diarrhea in Korea between the years 2007 and 2022

박영석,박준영,김중범,조승학 한국식품과학회 2024 Food Science and Biotechnology Vol.33 No.8

To investigate the molecular characteristics and antibiotic resistance of Staphylococcus aureus isolates from patients with diarrhea in Korea, 327 S. aureus strains were collected between 2007 and 2022. The presence of staphylococcal enterotoxin (SE) and toxic shock syndrome toxin-1 (TSST-1) genes in S. aureus isolates was determined by PCR. The highest expression of the TSST-1 gene was found in the GIMNO type (43.1% of GIMNO type). GIMNO type (Type I) refers to each staphylococcal enterotoxin (SE) gene gene (initials of genes): G = seg; I = sei; M = selm; N = seln; O = selo. Moreover, Type I isolates showed a significantly higher resistance to most antibiotics. A total of 195 GIMNO-type S. aureus strains were analyzed using multilocus sequence typing (MLST), and 18 unique sequence types (STs) were identified. The most frequent sequence type was ST72 (36.9%), followed by ST5 (22.1%) and ST30 (16.9%). Interestingly, ST72 strains showed a higher prevalence of MRSA than the other STs. In conclusion, our results were the first reported for S. aureus strains in Korea, which significantly expanded S. aureus genotype information for the surveillance of pathogenic S. aureus and may provide important epidemiological information to resolve several infectious diseases caused by S. aureus.

Record-Playback 기술 기반의 GUI 테스트 케이스 자동 생성

이정규(JungGyuew Lee),김현수(HyeonSoo Kim),국승학(SeunGhak Kuk),조대완(DaeWan Cho) 한국정보과학회 2007 한국정보과학회 학술발표논문집 Vol.34 No.1B

오늘날 GUI가 소프트웨어의 성공에 중요한 영향을 미침으로써 GUI에 대한 테스트가 요구된다. 반면에 소프트웨어의 GUI 테스트는 많은 노력과 시간이 소비된다. 이러한 자원의 소비를 줄이기 위해 GUI 테스트를 자동으로 수행하는 것이 필요하다. 본 논문에서는 GUI 자동 테스트를 수행하기 위해 극복해야 할 문제와 GUI 자동 테스트에 적용되는 기술에 대해 논하고, Record-Playback 기술을 이용하여 GUI 테스트케이스 자동 생성 방법을 제시한다.