저분자 수용성 닭가슴살 분말의 PDCAAS 분석을 통한 단백질 품질평가

이진아(Jin A Lee),김민주(Min Ju Kim),신미래(Mi-Rae Shin),노성수(Seong-Soo Roh),이중복(Jung-Bok Lee),서예희(Ye hei Seo),최현규(Hyun Gyu Choi),박해진(Hae-Jin Park) 한국식품영양과학회 2022 한국식품영양과학회지 Vol.51 No.5

본 연구는 수용성 저분자 가수분해 닭가슴살 파우더를 개발하여 섭취에 용이하게 하고 이의 조단백 함량, 아미노산 조성, 펩타이드 함량 비교 및 단백질 질(PER, NPR 및 PDCAAS 등) 분석을 통해 영양학적으로도 우수한 단백질 급원으로의 유용성을 증명하고자 하였다. LWCP의 중량 평균 분자량은 675로 ISP 2,448 및 WPI 9,626에 비교하여 월등히 작게 나타났다. 필수아미노산의 함량은 단백질 건강기능식품의 아미노산 스코어 환산을 위한 기준 필수아미노산 조성표(건강기능식품의 기준 및 규격 고시 제2021-25호)를 기준으로 필수아미노산 전 항목에서 기준 필수아미노산 조성함량을 초과하였다. LWCP는 in vitro 단백질 소화흡수율의 경우 99~98%로 대부분 소화 흡수되었으며, 단백질 질 평가를 위하여 실시한 단백질 효율의 경우 비교군인 ISP군보다 유의적으로 높게 나타났고 순단백질 이용률 및 단백질 순 소화율은 비교군 간 유의적 차이가 나타나지 않았다. 아미노산 점수의 경우 LWCP는 트립토판으로 1.33, ISP는 메티오닌과 시스테인의 합으로 1.14, WPI의 경우 히스티딘 1.03, 카제인의 경우 메티오닌과 시스테인의 합으로 1.01로 측정되어 모두 1 이상을 기록하였으며 LWCP의 아미노산 점수가 가장 높은 것으로 나타났다. 따라서 PDCAAS는 LWCP가 128.8%로 모든 식이 비교군과 비교하여 유의적으로 높은 값을 나타내었다. LWCP의 기능성 펩타이드로 측정한 안세린과 카르노신의 경우 각각 21.06 mg/g, 5.46 mg/g으로 나타났으며, ISP 및 WPI는 검출되지 않았다. 결론적으로 LWCP는 저분자 단백질로써 ISP 또는 WPI 군과의 in vitro 및 in vivo 단백질 소화흡수율에서 비교우위에 있어 단백질 질적인 측면뿐만 아니라 생리 기능성 측면에서도 우수하다고 판단되므로 고부가가치 신규 단백질 소재로써의 활용이 기대된다. This study was conducted to evaluate the protein quality of low-molecular weight water-soluble chicken breast powder (LWCP) by the protein digestibility corrected amino acid score (PDCAAS), which is a combination of the chemical score of the limiting amino acid multiplied by the true digestibility of the protein. The LWCP was hydrolyzed using by foodpro<SUP>®</SUP> alkaline protease for 4 hours at 55°C and was then further hydrolyzed with a combination of three enzymes (prozyme 2000P, bromelain, and papain) for 2 hours. Gel permeation chromatography (GPC) was used to measure the molecular weight distribution of the LWCP, isolated soy protein (ISP), and whey protein isolate (WPI). The protein quality of the LWCP was evaluated and compared with vegetable protein (ISP) and two animal proteins (WPI and casein). The protein quality was evaluated using a rodent bioassay of the protein digestibility and the amino acid composition of the LWCP was determined via high-pressure liquid chromatography (HPLC). Test diets were supplied for five days after an adaptation period of 4 days, to an animal model comprising 21∼28 days old SD rats with an average body weight of 70∼80 g. During the balance period, the nitrogen contents in the feces were assayed. As a result of estimating the weighted average molecular weight using GPC, the LWCP was observed to have a smaller value (Mw 675) than those of the ISP (Mw 995) and WPI (Mw 6,666). The protein efficiency ratio and net protein ratio of the LWCP were 2.9 and 4.9, respectively. The PDCAAS value of the LWCP was 128.8% which was significantly higher than those of the ISP and WPI. These results suggest that LWCP appears to be a promising protein source with good biological values and digestibility.

WAVE, VMS, SMS를 이용한 통합 사고 전파 시스템의 설계 및 구현

이진서(Jin-Seo Lee),김지훈(Ji-Hun Kim),박준우(Jun-Woo Park),김영덕(Young-Duk Kim) 대한전기학회 2020 정보 및 제어 심포지엄 논문집 Vol.2020 No.10

사군자탕(四君子湯)의 항염증 효능 연구

이진아,하혜경,정다영,이호영,이남헌,이준경,서창섭,김정훈,이미영,신현규,Lee, Jin-Ah,Ha, Hye-Kyung,Jung, Da-Young,Lee, Ho-Young,Lee, Nam-Hun,Lee, Jun-Kyoung,Seo, Chang-Seob,Kim, Jung-Hoon,Lee, Mee-Young,Shin, Hyeun-Kyoo 대한한의학방제학회 2010 大韓韓醫學方劑學會誌 Vol.18 No.1

Objectives : To provide the information of efficacy for Sagunja-tang (Sijunzi-tang; SG), it was evaluated the anti-inflammatory effect. SG, a widely used herbal formula in tranditional Korean medicine, has been used to treat for the Boki-invigorating. In many studies, plant-derived anti-inflammatory efficacies have been investigated for their potential inhibitory effects on lipopolysaccharide (LPS)-stimulated macrophages. This study was performed to examine the anti-inflammatory effects of SG extract on LPS-stimulated RAW 264.7 cells. Methods : The productions of nitric oxide (NO), prostaglandin (PG)$E_2$, interleukin (IL)-6 and tumor necrosis factor (TNF)-$\alpha$ were examined in a macrophage cell line, RAW 264.7 cells, in the presence of the SG extract. RAW 264.7 cells were incubated with LPS $1\;{\mu}g/mL$ and SG extract for 18 hours. The anti-inflammatory activity of SG was investigated by carrageenin-induced paw edema in rats. The paw volume was measured at 0, 2 and 4 hours following carrageenin-induced paw edema in rats. Results : SG extract showed inhibitory effect on $PGE_2$, IL-6 and TNF-$\alpha$ by LPS-stimulated RAW 264.7 cells. But SG extract was not inhibitory effect on NO by LPS-stimulated RAW 264.7 cells. And administration of SG extract (1 g/kg) showed a reduction in carrageenin-induced paw edema on rats. Conclusions : These results suggest that SG extract has anti-inflammatory activities in vitro and in vivo models.

사물 인터넷 환경에서의 그룹 사용자를 위한 그룹 구성 정보 기반 서비스 추천 방법

이진서(Jin-Seo Lee),고인영(In-Young Ko) Korean Institute of Information Scientists and Eng 2016 정보과학회논문지 Vol.43 No.7

Recommender systems can be used to assist users in selecting required services for their tasks in Internet of Things (IoT) environments in which diverse services can be provided by utilizing IoT devices. Traditional research on recommendation mainly focuses on predicting preferences of individual users. However, in IoT environments, not only individual users but also groups of users can access services in the environments. In this study, we analyzed user groups preferences on services and developed service recommendation approach for new groups that do not have a history of accessing IoT-services in a certain place. Our approach extends the traditional user-based collaborative filtering by considering the similarity between user groups based on their member organization. We conducted experiments with a real-world dataset collected from IoT testbed environments. The results demonstrate that the proposed approach is effective to recommend services to new user groups in IoT environments.

BIM기반 협업를 위한 협업시스템에서의 BCF 활용방안

이진강(Lee, Jin-Gang),이현수(Lee, Hyun-Soo),박문서(Park, Moon-Seo) 대한건축학회 2015 대한건축학회 학술발표대회 논문집 Vol.35 No.1

포스터 발표 : 최근 6년간 종합병원에 입원한 급성신우신염 환자의 임상적 양상과 원인균에 대한 고찰

이진서 ( Lee Jin Seo ),조성태 ( Jo Seong Tae ),오국환 ( O Gug Hwan ),윤종우 ( Yun Jong U ),김근호 ( Kim Geun Ho ),전로원 ( Jeon Lo Won ),김형직 ( Kim Hyeong Jig ),채동완 ( Chae Dong Wan ),노정우 ( No Jeong U ) 대한신장학회 2002 춘계학술대회 초록집 Vol.21 No.1

군 병원 환자와 간호사가 지각하는 시기별 간호요구도 : 정형외과 하지수술환자를 중심으로

이진이 ( Lee Jin-yi ),한자경 ( Han Ja-kyoung ),이문선 ( Lee Moon-sun ),박가현 ( Park Ga-hyeon ),서건강 ( Seo Geon-gang ) 국군간호사관학교 군진간호연구소 2017 군진간호연구 Vol.35 No.1

Purpose : This study was designed to identify the nursing needs of patients and nurses, to examine the difference of nursing needs between patients and nurses in military hospital. Methods : This study followed a cross-sectional survey design. The convenience sampling was used to recruit the participants form four military hospitals. The self-reported questionnaire was then used to survey nursing needs. Finally, t-test, ANOVA was used to analyze the responses of 150 patients and 99 nurses. Results : Nurses` nursing needs scores (4.07±0.45) were higher than patients` nursing needs scores (3.92±0.61). At the postoperation state, there is different nursing needs between patients and nurses (t=-3.190, p=.002). Conclusion : This study suggested further research about nursing needs between patients and nurses in military hospital, and adequate nursing intervention at the preoperation, postoperation and discharge state. It is necessary to develop a tool to examine nursing needs reflecting the military base. The key findings of this study can be a strategy of quality improvement.

Acetobacter orientalis MAK88 균주를 이용한 양파 식초의 발효 최적화

이진아 ( Jin-a Lee ),이설희 ( Sulhee Lee ),박영서 ( Young-seo Park ) 한국산업식품공학회 2017 산업 식품공학 Vol.21 No.4

양파를 이용하여 식초를 제조하기 위하여 막걸리로부터 분리된 내산성과 내알코올성이 우수하고 산생성능이 높은 Aectoacter orientalis MAK88를 사용하였다. 양파 추출액에 A. orientalis MAK88의 종배양액을 접종하여 초산 발효를 실시하였을 경우, 발효 최적 조건을 조사하였다. 앙파 추출액 중의 초기 에탄올 농도는 5% (v/v)가 최적이었으며, 발효 144시간에서의 산도는 4.31%이었다. 초기 초산 농도는 1% (v/v)가 최적으로 발효 144시간에서의 최종 산도는 5.32%였다. 최적 발효온도는 28℃로 확인되었다. 양파 착즙액은 통 양파를 121℃에서 15분간 가열한 뒤 가압 착즙후 여과한 다음 121℃에서 15분간 살균하여 제조한 것이 발효에 가장 적합하였으며, 희석하지 않은 착즙 원액을 사용하는 것이 가장 좋았다. Acetic acid bacteria strains were isolated from a variety of fermented foods and fallen fruits. Among them, the strain MAK88, whose acetic acid fermentation ability, acid-tolerance, and alcohol-tolerance were high, was selected and identified as Acetobacter orientalis. A seed culture of A. orientalis MAK88 was inoculated into onion juice, and the optimum conditions of acetic acid fermentation was investigated. The optimum initial concentration of ethanol in onion juice was 5% (v/v) and in that condition, acidity was 4.31% at 144 h of fermentation. The optimum initial concentration of acetic acid was 1% and the final acidity was 5.32%. The optimum fermentation temperature was determined to be 28℃. The most appropriate preparation method of onion juice was to heat the onion at 121℃ for 15 min and produce juice with pressure followed by filtering, and then sterilization at 121℃ for 15 min. Prepared onion juice was used for fermentation without dilution.

CEO의 비전설정 행위와 조직성과 간 조직시민행동의 매개효과 분석

이진서(Jin-Seo Lee),이홍배(Hong-Bae Lee) 한국산업경제학회 2013 산업경제연구 Vol.26 No.6

본 연구는 CEO의 비전설정 행위와 성과 간에 있어서 조직시민행동의 매개효과를 분석하기 위해 구조방정식의 구조모형을 설정하였다. 그리고 O"Connell et al.(2011)이 제시한 비전 설정의 유형을 토대로 다섯 가지 유형을 검토하였으며, 분산분석과 Duncan 다중비교분석을 통하여 세 가지 유형으로 재분류하고, 재분류된 유형에 따라 비전공유 정도 차이와, 비전설정-조직시민행동-성과의 구조모형에 대한 조절효과를 분석하였다. 분석결과, 비전설정과 성과 간에 조직시민행동의 매개효과를 확인하였다. 그리고 비전설정 수준에 따라 세 가지 유형으로 분류된 비전설정 유형의 분산분석과 Duncan 다중비교분석을 통하여 비전공유 차이를 확인하였으며, 비전설정-조직시민행동-성과의 구조모형에 있어서 비전설정 유형의 조절효과도 확인하였다. 비전설정 초기부터 조직구성원들과의 참여를 통하여 비전을 공유함으로써 단기적인 재무성과 이상의 역할 외 행동인 조직시민행동을 통해 조직의 순응적 태도는 말할 것도 없고, 개인적인 이타적 행동을 강화하고, 조직을 위한 혁신적 행동까지 이끌어 낼 수 있다는 점에서 매우 시사점이 크다고 판단된다. The purposes of this study is to analyze the effect of the vision creation on the OCB(Organizational Citizenship Behavior) and the performance, and to review the mediating effect of the OCB on the vision creation and the performance, and also to analyze the moderating effect of the three visioning type on the relation among the vision creation, OCB and the performance. We formulate the structural equation model(SEM) for analyzing the relations among the three construct or latent variables(the vision creation, OCB, performance). And we analysis the mediating effect of the OCB, and the moderating effect of the three visioning type re-classified by the ANOVA analysis. The empirical results are as follows. First, the vision creation has the statistically significant positive relation about the employee performance/finance performance through the mediating effect of the OCB. Second, we recognized the moderating effect of the three visioning type among the vision creation, OCB, and performance.

뷰티업소 선택에 있어 SNS 광고속성이 행동의도에 미치는 영향

이진서(Lee, Jin Seo),모정희(Mo, Jeong Hee) 한국디자인문화학회 2022 한국디자인문화학회지 Vol.28 No.2