인간포배기 배아의 효과적인 유리화 동결법의 개발을 위한 연구

이상민,이주희,이상원,이승민,윤산현,임진호,박흠대,이성구,Lee, Sang-Min,Lee, Ju-Hee,Lee, Sang-Won,Lee, Seoung-Min,Yoon, San-Hyun,Lim, Jin-Ho,Park, Huem-Dai,Lee, Seong-Goo 대한생식의학회 2003 Clinical and Experimental Reproductive Medicine Vol.30 No.3

Objective: The purpose of this study was to evaluate the survival rate of vitrified blastocyst according to the freezing vessels, equilibration time in cryoprotectant and artificial dehydration method. Methods: Human blastocysts were vitrified after loading onto the plastic straw, open-pulled straw (OPS), electron microscopy grid (EM grid) for 1.5 min or 3 min. They also were directly plunged into LN2 within 30sec. For artificial shrinkage of blastocysts, 36 gauge fine needle was pushed at the cellular junction of the trophectoderm into the blstocoele cavity until it shrank without damage of inner cell mass. Results: The survival rate of vitrified blastocysts on plastic straw, OPS, EM grid as freezing vessels were 26.7, 13.0 and 60.5%, respectively. The survival rate of EM grid was significantly higher than that of plastic straw and OPS (p<0.05). For 1.5 min equilibrium, the survival rates of early blastocyst (EB), middle blastocyst (MB) and late blastocyst (LB) were 64.4, 81.0, and 20.0% respectively. For 3 min equilibrium, the survival rates of EB, MB, and LB were 69.9, 50.0 and 57.5% respectively. The survival rates of EB and MB were significantly higher than that of LB in 1.5 min equilibrium group (p<0.05), however, the significance was not observed in 3 min equilibrium groups. In cytoplasmic shrinkage before vitrification, the survival rates of EB, MB and LB were 92.9, 100 and 75.9% respectively. The survival rate of MB was significantly higher than that of LB (p<0.05). The survival rates of vitrified blastocysts by artificial dehydration and slow-frozen blastocysts were not significantly different as 88.9 and 66.7%, respectively. Conclusion: This study showed that the vitrification of human blastocysts using EM grid and artificial dehydration is an effective method. Therefore, these methods would be an useful techniques for blastocyst cryopreservation.

헤어리베치(Vice villosa Roth) 품종별 국내 월동성, Biomass 및 무기태 질소 함량비교

이상민,이연,이용환,성좌경,윤홍배,최현석,Lee, Sang-Min,Lee, Youn,Lee, Yong-Hwan,Sung, Jwa-Kyung,Yun, Hong-Bae,Choi, Hyun-Sug 한국유기농업학회 2013 韓國有機農業學會誌 Vol.21 No.1

헤어리베치 8종의 품종별 국내 적응성 검증을 위해 월동률과 biomass, 그리고 토양 중 무기태질소를 조사하였다. 겨울철 월동 후 생존율은 Hungvillosa와 Ostsaat 품종에서 좋았고, Minnie, Oregon common과 TTF1에서 낮았다. 생존율은 biomass에도 영향을 주어서 Hungvillosa와 Ostsaat 품종에서 가장 높았다. 헤어리베치 품종 간 무기성분 농도는 별다른 차이가 관찰되지 않았다. 토양 중 무기태질소 농도는 질소환원능이 좋았던, Hungvillosa와 Ostsaat가 예초 후 10여일 후에 크게 증가시켰다. This study was conducted to evaluate overwintering of hairy vetch (Vicia villosa Roth), biomass, and inorganic N concentration in soil to verify the adaptability of eight cultivars of hairy vetch into country. Winter survival rate was higher for the hairy vetch cultivars, such as Hungvillosa or Ostsaat, than those of Minnie, Oregon common, and TTF1, which affected amount of biomass of each hairy vetch, with higher biomass observed in Hungvillosa or Ostsaat cultivar. There were no significant difference for the mineral nutrients of each hairy vetch cultivar. Inorganic N concentration in soil was increased at 10 days after mowing by application of Hungvillosa and Ostsaat cultivars that had highly produced N content from the raw materials.

녹색건축 전문가 자격제도 개발 연구

이상민,이승민,Lee, Sang Min,Lee, Seung Min 한국교육녹색환경연구원 2014 교육·녹색환경연구 Vol.13 No.1

In an effort to resolve environmental pollution problems, developed countries enforce green building certification systems. The same is true in Korea. However compared professional accreditation system to certification systems did not systematically in Korea. Therefore, in accordance with development of green building related systems and technology the requirement for foster green building professionals is urgent. Accordingly this study is purposed to analyze and propose that green building professional accreditation system development and operation of the direction. Through data analysis domestic and international green building professional accreditation system for draw a development plan. And then conduct an expert Delphi survey reflected in the survey results to development plan.

대식세포주에서 인슐린이 $I{\kappa}B/NF-{\kappa}B$ 경로 활성화에 미치는 영향

이상민,장연실,이춘택,김영환,한성구,심영수,유철규,Lee, Sang-Min,Jang, Yeon-Sil,Lee, Choon-Taek,Kim, Young-Whan,Han, Sung-Koo,Shim, Young-Soo,Yoo, Chul-Gyu 대한결핵및호흡기학회 2010 Tuberculosis and Respiratory Diseases Vol.68 No.3

Background: Sepsis still has a high mortality rate despite adequate supportive care. Newer therapeutic modalities have been developed but they have generally ended in failure. Recently, insulin was reported to have an anti-inflammatory effect by inhibiting the $I{\kappa}B/NF-{\kappa}B$ pathway, and may have therapeutic potential in sepsis. However, the precise mechanism of the anti-inflammatory effect of insulin is unclear. This study examined the role of insulin in activating $I{\kappa}B/NF-{\kappa}B$ in macrophage. Methods: Raw 264.7 cells, a murine macrophage cell line, were used in this experiment. Western blotting using $I{\kappa}B$ Ab and phosphor-specific $I{\kappa}B$ Ab was performed to evaluate the degradation and phosphorylation of $I{\kappa}B$ cells. For the $I{\kappa}B$ Kinase (IKK) activity, an immune complex kinase assay was performed. The level of interleukin-6 (IL-6) was measured by ELISA to determine the level of proinflammatory cytokine. Results: $I{\kappa}B{\alpha}$ degradation began 30 min after lipopolysaccharide (LPS) treatment. However, an insulin pretreatment suppressed the $I{\kappa}B{\alpha}$ degradation caused by the LPS treatment. The phosphorylation of $I{\kappa}B{\alpha}$ and IKK activity was also inhibited by the insulin pretreatment. Finally, the insulin pretreatment showed a tendency to suppress the induction of IL-6 by LPS. Conclusion: Insulin might have an anti-inflammatory effect though partial inhibition of the $I{\kappa}B/NF{\kappa}B$ pathway in macrophage cell lines.

대장직장 종양에 대한 내시경 점막하 절제술의 연대순 임상 결과와 학습 곡선

이상민 ( Sang Min Lee ),김은수 ( Eun Soo Kim ),박경식 ( Kyung Sik Park ),조광범 ( Kwang Bum Cho ),김동춘 ( Dong Choon Kim ),강유진 ( Yu Jin Kang ),이유진 ( Yoo Jin Lee ),이정민 ( Jung Min Lee ),최은성 ( Eun Sung Choi ),최재혁 ( Ja 대한소화기학회 2014 대한소화기학회지 Vol.64 No.4

Background/Aims: Endoscopic submucosal dissection (ESD) is an effective procedure for en-bloc curative resection of the colorectal tumor. As it requires high technical skills and experience in therapeutic endoscopy, it is important to understand learning curve of ESD technique. The aim of this study was to retrospectively describe the clinical results of ESD and to evaluate learning curve for the ESD of colorectal tumors. Methods: A total of 90 patients with 90 colorectal neoplasms, who had undergone ESD at a tertiary referral hospital from July 2009 to December 2012, were enrolled. The ESD was performed by a single endoscopist. All ESD cases were divided into three periods: first, cases 1-30; second, cases 31-60; and third, cases 61-90. Results: The en-bloc resection rates in third period (100%) was significantly higher than that of the first (93.3%) and second period (80%) (p=0.025). The perforation rate in third period (0%) also significantly decreased compared with that of the first (13.3%) and second period (20%) (p=0.032). To calibrate the difference of tumor size among periods, proficiency was calculated, as the procedure time per specimen area (min/cm2). The proficiency in third period (4.3) was significantly shorter than that of the first (16.8) and second period (10.2) (p=0.004). Conclusions: The learning curve of colorectal ESD in our study shows that at least 60 cases of ESD have to be conducted to acquire sufficient skill of degree without perforation. (Korean J Gastroenterol 2014;64:198-205)

녹비작물을 이용한 윤작이 잡초 제어에 미치는 영향

이상민,이병모,이연,이용환,성좌경,윤홍배,최현석,Lee, Sang-Min,Lee, Byung-Mo,Lee, Youn,Lee, Yong-Hwan,Sung, Jwa-Kyung,Yun, Hong-Bae,Choi, Hyun-Sug 한국유기농업학회 2012 韓國有機農業學會誌 Vol.20 No.2

본 시험은 유기농업 파 및 고추재배를 위하여 호밀과 헤어리베치를 겨울에 윤작하여 잡초 발생량 억제에 미치는 효과를 검토하기 위하여 수행되었다. 파 재배구에서 호밀을 토양에 환원하지 않은 처리구에 비해 호밀을 환원하였을 경우 잡초발생량을 52% 경감시켰다. 고추 재배구에서는 그러한 효과가 뚜렷하게 관찰되지 않았다. 파 재배시 어저귀, 참방동사니, 바랭이와 같은 초종은 호밀뿌리 분비물에 의해 발아가 억제되는 경향이었으나 헤어리베치는 그러한 발생억제 효과가 크지 않았다. 파 재배시 호밀 환원은 잡초발생량을 경감시켰고 잡초의 종 다양성 지수를 증가시켰다. This study was conducted to evaluate the effects of crop rotation on the weed occurrence in green onion and red pepper cultivation. Rye and hairy vetch were annually seeded for winter season. The reduction of weed germination was pronounced by about 50% in green onion field, but was not significant in red pepper field through rye cropping. Among weed species, the germination of Abutilon theophrasti, Cyperus iria and Digitaria ciliaris was distinctly inhibited by exudate of rye root, but not by that of hairy vetch in vitro experiment. The return of rye biomass into onion field decreased amount of weed biomass but increased index of weed diversity.

건강식생활 유형 및 건강증진 동기가 힐링체험 욕구에 미치는 영향

이상민,윤지영,정희선,Lee, Sang-Min,Yoon, Jiyoung,Jeong, Hee Sun 한국식품조리과학회 2015 한국식품조리과학회지 Vol.31 No.4

The purpose of this study was to examine the effects of a lifestyle consisting of a health oriented diet and personal motivation toward health improvement on an individual's desire to participate in condolence or "healing" -workshops and programs. In order to examine the influence of the "health diet" and motivation, the "health diet" lifestyle was classified into nutrition pursuit factors, psychological factors, food safety factors, and health conscious consumption factors. Personal motivation was categorized into perceived benefits, environmental factors and the individual's desire for happiness. Empirical analysis showed that nutrition pursuit factors had an impact on perceived benefits and the desire for happiness. Psychological aspect factors had a significant influence on perceived benefits, environmental factors and the desire for happiness. However, food safety factors and health conscious consumption factors did not have an effect on individual motivation to improve health. Regarding personal motivation toward health improvement on the desire for "healing", perceived benefits and environmental factors had significant influence. However, the individual's desire for happiness did not influence the demand for "healing". Regarding the effect of a "health diet" on the desire for "healing", only the psychological aspects of a "health diet" had any significant effects on the desire for "healing". The results from this study can help local organizations or service companies that provide "healing" programs with developing effective marketing strategies that could lead to greater customer satisfaction. Especially, the significant influence of psychological aspects of a "health diet" on the demand for "healing" is noteworthy in that this could play a key role in establishing a more effective marketing strategy to attract increasing numbers of consumers using health conscious diets.

지르코니아 단일구조 전부도재관과 금속도재관의 파절강도 비교

이상민,정희찬,전영찬,Lee, Sang-Min,Jeong, Hee-Chan,Jeon, Young-Chan 대한치과보철학회 2007 대한치과보철학회지 Vol.45 No.1

Purpose: The purpose of this study was to compare the fracture strength of the zirconia monolithic all-ceramic crowns according to the thickness (0.5mm, 0.8mm, 1.1mm) and metal-ceramic crowns (1.0mm, 1.5mm) Material and method: Twelve crowns for each of 3 zirconia crown groups were fabricated using CAD/CAM system (Kavo, Germany) and twelve crowns for each of 2 metal-ceramic crown groups were made by the conventional method. All crowns were luted to the metal dies using resin cement. Half of the specimens were exposed to thermocycling ($5-55^{\circ}C$, 1 Hz) and cyclic loading (300,000 cycles, 50N). Subsequently, all crowns were mounted on the testing jig in a universal testing machine. The load was directed at the center of crown with perpendicular to the long axis of each specimen until catastrophic failure occurred. Analysis of variance and Tukey multiple comparison test (P<.05) were used for statistical analysis of all groups, and paired t-test (P<.05) was followed for statistical comparison between each groups' fracture load before and after cyclic loading. Results: 1. The fracture strength of the zirconia monolithic crowns and the metal-ceramic crown increased as thickness increased (P<.05). 2. The cyclic loading and thermocycling significantly decreased the fracture strength of the zirconia monolithic crowns (P<.05). 3. The standard deviation of fracture strength of the zirconia monolithic crowns was very low. Conclusion: The fracture strength of the zirconia monolithic crowns for the posterior area tends to be higher with thickness increased and 0.8mm or over in thickness is recommended to have similar or over the fracture strength of metal-ceramic crowns.

머신러닝 기법을 활용한 낙동강 중류 지역의 Chl-a 예측 알고리즘 비교 연구(수질인자 및 수량 중심으로)

이상민,박경덕,김일규,Lee, Sang-Min,Park, Kyeong-Deok,Kim, Il-Kyu 대한상하수도학회 2020 상하수도학회지 Vol.34 No.4

In this study, we performed algorithms to predict algae of Chlorophyll-a (Chl-a). Water quality and quantity data of the middle Nakdong River area were used. At first, the correlation analysis between Chl-a and water quality and quantity data was studied. We extracted ten factors of high importance for water quality and quantity data about the two weirs. Algorithms predicted how ten factors affected Chl-a occurrence. We performed algorithms about decision tree, random forest, elastic net, gradient boosting with Python. The root mean square error (RMSE) value was used to evaluate excellent algorithms. The gradient boosting showed 10.55 of RMSE value for the Gangjeonggoryeong (GG) site and 11.43 of RMSE value for the Dalsung (DS) site. The gradient boosting algorithm showed excellent results for GG and DS sites. Prediction value for the four algorithms was also evaluated through the Receiver operating characteristic (ROC) curve and Area under curve (AUC). As a result of the evaluation, the AUC value was 0.877 at GG site and the AUC value was 0.951 at DS site. So the algorithm's ability to interpret seemed to be excellent.

소양인(少陽人) 임신오조(姙娠惡阻) 환자를 형방사백산(荊防瀉白散)으로 치료한 치험례

이상민,배영춘,유관석,주종천,서은희,Lee, Sang-Min,Bae, Young-Chun,Yoo, Gwan-Seok,Joo, Jong-Cheon,Seo, Eun-Hee 사상체질의학회 2004 사상체질의학회지 Vol.16 No.2

1. Objectives Hyperemesis Gravidarum is one of common symptoms clinically and caused by pregnancy. This is defined as vomiting sufficiently pernicious to produce weight loss, dehydration, acid-base imbalance. In severe case, it also leads to neurologic abnormality including confusion, gate disturbance, nystagmus. We have tried researching effect of herbal medicines, Soyangin Hyungbangsabaek-san. 2. Methods To heal the Hyperemesis Gravidarum, we used Soyangin Hyungbangsabaek-san. 3. Results Hyperemesis Gravidarum of a Soyangin patient was treated with a herb-medicine, and change of her subjective symptom & general condition was managed. 4. Conclusions The constitutional treatment with herb-medicine (Soyangin Hyungbangsabaek-san) was efficacious against Hyperemesis Gravidarum. Further study on management of this disease is needed.