시간경로 유전자 발현자료에서 패턴일치지수와 적응 최근접 이웃을 활용한 결측값 대치법

신혜서,김동재 한국통계학회 2020 응용통계연구 Vol.33 No.3

Time course gene expression data is a large amount of data observed over time in microarray experiments. This data can also simultaneously identify the level of gene expression. However, the experiment process is complex, resulting in frequent missing values due to various causes. In this paper, we propose a pattern consistency index adaptive nearest neighbors as a method of missing value imputation. This method combines the adaptive nearest neighbors (ANN) method that reflects local characteristics and the pattern consistency index that considers consistent degree for gene expression between observations over time points. We conducted a Monte Carlo simulation study to evaluate the usefulness of proposed the pattern consistency index adaptive nearest neighbors (PANN) method for two yeast time course data. 시간경로 유전자 발현 자료는 마이크로어레이 실험을 시간에 따라 관측한 대용량의 자료로 유전자 발현 수준을 동시에 파악할 수 있다. 하지만 실험 과정이 복잡하여 다양한 원인들에 의해 결측값이 자주 발생한다. 본 논문에서는 시간경로 유전자 발현 자료에 대한 결측값을 추정하는 방법으로 패턴 적응 최근접 이웃(pattern consistency index adaptive nearest neighbors; PANN) 방법을 제안하였다. 이 방법은 국소적 특징을 반영하는 적응 최근접 이웃(adaptive nearest neighbors; ANN) 방법과 관측 시점간 유전자 발현의 일치 정도를 고려하는 패턴일치지수를 결합시킨 것이다. 제안한 PANN 방법의 효능을 평가하기 위하여 두 가지의 실제 시간경로 자료들을 사용하여 몬테카를로 모의실험(Monte Carlo simulation study)을 시행하였다.

장수풍뎅이 유충(Allomyrina dichotoma Larva) 저온 추출물의 과산화수소로 유도된 산화 스트레스에 대한 H9c2 Cell 사멸 보호 효과

신혜지(Hye-Ji Shin),김화선(Hwa-Seon Kim),홍준표(Jun-Pyo Hong),변의홍(Eui-Hong Byun) 한국식품영양과학회 2023 한국식품영양과학회지 Vol.52 No.10

제로에너지수준의 그린리모델링을 위한 단열공사의 공사비 예측 모델 개발

신혜리,이승언,홍은빈,장예은,임지연 한국건축친환경설비학회 2021 한국건축친환경설비학회 논문집 Vol.15 No.6

This study conducted a cost prediction model that minimizes project risk and enables rational decision-making by predicting additional construction costs for insulation work. The essential factors for building energy efficiency have to evaluate in the early stages of the green remodeling project. Criteria for each building’s semi-performance for green remodeling of zero energy level defined, detailed classification items and standards established, and conditions of five groups categorized according to the semi-performance degree. The prediction model consists of three stages: 1) existing building information, 2) insulation material composition, and 3) construction cost derivation. The construction cost consists of 21 insulation items according to the legal standards of existing buildings, calculates material costs, labor costs, and cost. This prediction model constructed a more substantial construction cost calculation model from the perspective of construction and construction costs is possible to present an optimal construction cost scenario according to options such as semi-performance, structure, and region during green remodeling.

돼지 난자의 체외성숙에서 합성배양액에 첨가된 과당이 난자의 성숙 및 단위발생 배아의 체외발육에 미치는 영향

신혜지,김민지,이주형,이승태,박춘근,현상환,이은송 한국수정란이식학회 2017 한국동물생명공학회지 Vol.32 No.3

The objective of this study was to determine the effect of fructose that was supplemented to a chemically defined in Vitro maturation (IVM) medium on oocyte maturation and embryonic development after parthenogenesis in pigs. The base medium for in Vitro maturation (IVM) was porcine zygote medium (PZM) that was supplemented with 0.05% (w/v) polyvinyl alcohol (PVA) or 10% (v/v) porcine follicular fluid (pFF). In the first experiment, when immature pig oocytes were matured in a chemically defined medium that was supplemented with 5.5 mM glucose or with 1.5, 3.0 and 5.5 mM fructose, 3.0 mM fructose resulted in a higher nuclear maturation (91.5%) than 1.5 and 5.5 mM fructose (81.9 and 81.9%, respectively) but showed a similar result with 5.5 mM glucose (94.2%). However, there was no significant differences among groups in the embryo cleavage (89.4-92.4%), blastocyst formation (37.5-41.1%), and mean cell number of blastocyst (30.8-34.2 cells). Fructose at the concentration of 3.0 mM (1.08 pixels/oocyte) resulted in a higher intra-oocyte glutathione (GSH) content than 1.5 and 5.5 mM fructose (1.00 and 0.87 pixels/oocytes, respectively) while the cumulus cell expansion was not influenced. In the second experiment, effect of individual and combined supplementation of a chemically defined maturation medium with 5.5 mM glucose or 3.0 mM fructose was examined. No significant effect was found in the nuclear maturation (86.3-92.6%). Embryo cleavage was significantly increased by the combined supplementation with glucose and fructose (95.2%) compared to that with 3.0 mM fructose only (85.7%) while blastocyst formation (37.3-42.8%) and embryonic cell number (33.3-34.1 cells) were not altered. Effect of supplementation of pFF-containing medium with glucose and fructose + glucose was examined in the third experiment. No significant effect by the supplementation with glucose and fructose or glucose alone was observed in the nuclear maturation of oocytes (90.7-94.1%) and blastocyst formation (51.0-56.5%). Our results demonstrate that 3.0 mM fructose was comparable to 5.5 mM glucose in supporting in Vitro oocyte maturation and embryonic development after parthenogenesis and could be used as an alternative energy source to glucose for in Vitro maturation of pig oocytes.

건물부문 화석연료 전환을 위한 서울지역 아파트의 도시가스를 이용한 취사에너지 사용 특성 분석

신혜리,김유민 한국에너지기후변화학회 2023 에너지기후변화학회지 Vol.18 No.2

To achieve carbon neutrality in the building sector by 2050, a reduction of direct emissions is necessary, and they are generated by the combustion of fuel. In the building sector, it involves the direct combustion of gases such as briquettes, heating oil, LPG, and city gas, and is mainly used for heating, hot water supply, and cooking. While the proportion of energy used for heating and hot water is expected to decrease by 2050, but that used for cooking is expected to increase relatively. Accordingly, this study analyzed cooking energy consumption using city gas for apartment complexes in Seoul and presented trends in cooking city gas consumption according to building area and year. In addition, by presenting the characteristics through analysis of each local government, we attempted to provide basic data that can set reduction goals according to the characteristics of each local government.

건물에너지 공공검진 서비스를 위한 생애주기 지원 체계 설계

신혜리(Shin, Hyery),김유민(Kim, Yumin),이승언(Lee, Seung-Eon) 대한건축학회 2022 대한건축학회 학술발표대회 논문집 Vol.42 No.2

According to the The Republic of Korea’s Enhanced Update 2030 NDC announcement in October 2021, In the building sector alone, a reduction of 32.8%(17.1 million tons, direct sector) by 2030 is required. Energy efficiency of existing buildings amounting to about 7.2 million buildings is essential to achieve greenhouse gas reduction in the building sector. In this study, support system in the medical field was benchmarked to design a Building Energy Public check-up service tailored to the life cycle for buildings nationwide. The service is expected to contribute to reducing greenhouse gas emissions in the building sector and reducing the cost of building energy efficiency through preventive support for building energy efficiency.

장수풍뎅이 유충(Allomyrina dichotoma Larvae) 저온 추출물의 MAPKs 및 NF-κB 신호전달을 통한 대식세포 내 면역 증진 효과

신혜지(Hye-Ji Shin),김화선(Hwa-Seon Kim),변의홍(Eui-Hong Byun) 한국식품영양과학회 2024 한국식품영양과학회지 Vol.53 No.3

본 연구는 5°C에서 추출한 장수풍뎅이 유충 저온 추출물(ADL)의 면역 활성을 평가하고, 면역 증강 관련 기능성 소재로서의 활용 가능성을 평가하고자 대표적인 대식세포로 알려진 RAW264.7 cell을 활용하여 면역 활성을 측정하였다. 이에 앞서 우수한 단백질 및 총 폴리페놀 함량(TPC)을 나타내는 추출 온도를 알아내고자 BCA assay 및 TPC 측정법을 진행하였다. 5°C 및 75°C에서 추출한 ADL에 대해 평가한 결과, 5°C ADL이 75°C ADL 대비 뛰어난 단백질 및 TPC를 나타내었다. Cell viability에 대하여 측정한 결과, 모든 농도에서 세포독성이 나타나지 않았으며, 5°C ADL이 75°C ADL 대비 높은 세포 생존율을 나타내어 추후 실험에서 5°C ADL로 고정하여 진행하였다. 이후 사이토카인(IL-6, IL-1β) 분비능 및 면역 활성 신호전달 경로를 측정하였으며, 농도별(100, 200 μg/mL) 5°C ADL의 처리에 따라 두 사이토카인(IL-6, IL-1β)의 분비능이 농도 의존적으로 증가하는 것을 확인하였다. 더불어 5°C ADL의 처리가 MAPKs family의 인산화 및 NF-κB 전사 기전을 통해 대식세포 내 면역 활성 증가를 유도하는 것을 확인할 수 있었다. 이러한 연구 결과를 통하여 장수풍뎅이 유충 저온 추출물이 면역활성을 증강시키는 기능성 소재로서의 가능성을 확인하였지만, 이와 관련된 정확한 생리활성을 나타내는 유효성분을 명확히 규명할 수 없으므로 후속 연구가 필요한 실정이다. Allomyrina dichotoma larvae are a promising alternative protein resources for foods. Although nutrients are abundant in A. dichotoma larvae and exhibit various physiological functions, nothing is known of the immune activities of A. dichotoma larvae extracts at low temperatures (5°C). This study investigated the immune-enhancing activity of A. dichotoma larvae extract (ADL) at low-temperatures in RAW264.7 cells, a reprensentative macrophage cell line. ADL (5°C) was not cytotoxic at 100 or 200 μg/mL and increased interleukin-6, interleukin-1β production at both concentrations. Furthermore, these immunomodulatory effects were mediated by the phosphorylations of MAPKs (p38, JNK, and ERK) and the translocation of NK-ĸB. Consequently, the study shows that ADL at low-temperature can be used as an immunity-enhancing ingredient.

Identification of Antioxidative Constituents from Polygonum aviculare using LC-MS Coupled with DPPH Assay

신혜지,정하연,박병덕,이기용 한국생약학회 2016 Natural Product Sciences Vol.22 No.1

A method for simultaneously identifying antioxidative compounds was developed using time-based LC-MS coupled with DPPH assay regardless of the time consuming process. The methanolic extract of Polygonum aviculare (Polygonaceae) showed significant DPPH radical scavenging activity. Time-based DPPH assay for simultaneous identification of active compounds from the extracts of P. aviculare was used. Major peaks of ethyl acetate fraction of P. aviculare showed high DPPH radical scavenging activity. A simple phenolic compound (1) and six flavonoids (2-7) were isolated from the ethyl acetate fraction of P. aviculare by silica gel and sephadex LH-20 column chromatography. The structures of seven compounds were determined to be protocatechuic acid (1), catechin (2), myricitrin (3), epicatechin-3-O-gallate (4), avicularin (5), quercitrin (6), and juglanin (7) based on the analysis of the 1H-NMR, 13C-NMR and ESI-MS data. All compounds exhibited significant antioxidant activity on DPPH assay and active compounds were well correlated with predicted one.

지역사회고령친화성이 노인의성공적 노화에 미치는 영향: 베이비부머와 노인 세대의 비교를 중심으로

신혜리,이혁준,임진섭 경인행정학회 2018 한국정책연구 Vol.18 No.4

This purpose of this study is to examine the age-friendly community affects successful aging, by comparison between baby boomer group and aged group. Using age-friendly and aged survey data in ◌◌◌◌◌ city’s which performed from ◌◌◌◌◌ University age-friendly lab, and analyzed PSM(Propensity Score Matching), Frequency, T-test and Multi-regression. The result showed that aged group get high score age-friendly score and successful aging score compared with baby boomer group. This study result suggest that development of age integration’s view is important to make a age-friendly community and successful aging. Also, Baby-boomer’s need to special preparation for later life program who different from previous old generation 본 연구는 지역사회고령친화성이 노인의 성공적 노화에 미치는 영향을 검증하는 것이며 동시에 이에 따른 베이비부머와 노인 세대의 집단별 차이를 살펴보고자 한다. 이를 위해 ◌◌대학교 ◌◌◌◌◌◌연구소에서 수행한 ◌◌◌◌◌고령친화성 및 거주노인 실태조사(2017)자료를 이용하였으며, 분석방법으로는 성향점수매칭, 빈도분석 및 T-test, 그리고 다중회귀분석을 실시하였다. 본 연구의 분석결과, 노인 집단이 베이비부머에 비해 고령친화성 정도 및 성공적 노화 정도가 모두 높게 나타났음을 확인하였다. 이러한 연구결과를 통해 본 연구에서는 첫째, 전 세대를 아우를 수 있는 연령통합적 관점에서의 고령친화적 지역사회 개발을 제안하였다. 둘째, 이전 노인세대와는 특성이 전혀 다른 베이비부머를 위한 별도의 노후준비 또는 노후생활 프로그램의 개발 및 제공을 제안하였다.

과부담 의료비 지출이 미충족 의료경험에 미치는 영향

신혜리(Shin Hye Ri),임예직(Lim Ye Gick),한기명(Han Ki Myung) 한국지역사회학회 2014 지역사회연구 Vol.22 No.3

This study examines the relations between medical expenditure and unmet needs for health care by investigating the moderating effect of the private health insurance. Logistic analysis was executed using Korea Welfare Panel Study (KOWEPS) to investigate the relations and influences among variables. The results of this study can be summarized as follows: First, medical expenditure is commonly positively associated with unmet needs for health care. Second, private health insurance is negatively associated with unmet needs for health care. Finally, private health insurance moderates in between the relation of medical expenditure and unmet needs for health care. This study has an important policy implication on expansion of public health policy.