9-12세 정상 아동에서 종골 골밀도와 체성분의 연관성

신은경,김기숙,김희영,이인숙,정효지,조성일,Shin, Eun-Kyung,Kim, Ki-Suk,Kim, Hee-Young,Lee, In-Sook,Joung, Hyo-Jee,Cho, Sung-Il 대한예방의학회 2004 예방의학회지 Vol.37 No.1

Objectives : This cross-sectional study aimed to quantify the relationship between the bone mineral density at the os calcis and the body mass composition in healthy children. Methods : The areal bone mineral density was measured at the os calcis with peripheral dual energy X-ray absorptiometry. The fat free mass, fat mass and percentage fat mass were measured using bioelectric impedance, in 237 Korean children, aged 9 to 12 years. The sexual maturity was determined by self assessment, using standardized series of the 5 Tanner stage drawings, accompanied by explanatory text. Results : From multiple linear regression models, adjusted for age, sexual maturity and height, the fat free mass was found to be the best predictor of the calcaneal bone mineral density in both sexes. About 15 and 20% variabilities were found in the calcaneal bone mineral densities of the boys and girls, respectively, which can be explained by the fat free mass. After weight adjustment, the percentage fat mass was negatively associated with the calcaneal bone mineral density in both sexes. Conclusions : The findings of this study suggest that the fat free mass, among the body compositions, is the major determinant of bone mineral density at the os calcis in Korean children aged 9 to 12 years. Obesity, defined as the percentage fat mass, is assumed to have a negative effect on the calcaneal bone density in children of the same weight.

KS 시설관리 표준규격을 바탕으로 한 대학시설 FM 서비스 발전방향

신은경,김유진,김준하,Shin, Eun-Young,Kim, Yu-Jin,Kim, Jun-Ha 한국교육시설학회 2015 敎育施設 Vol.22 No.1

Over the years, great attention has been shown to the function of the campus facilities that should be well-equipped, operated and maintained to better support university's two main goals: providing education and supporting research. Properly managing campus facilities is the initial step to be prepared to meet the rapidly changing global educational standard. However, so far, no definitive answer has been given to the question of what service level/scope is appropriate for the effective campus facility management (FM) in Korea. Therefore, at the outset, it is imperative to establish the framework for the standardized campus FM services. The main focus of this research is to provide comprehensive understanding on the campus FM by establishing the framework in the form of FM performance indicator (PI). As the first step, FM standards set by Korean Agency for Technology and Standards (KATS) has been thoroughly reviewed and analyzed to establish the framework for FM standards applicable to campus facility management. Secondly, extensive literature review on the development of performance indicator (PI) for the educational facilities has been conducted to classify PI types and complement FM_KS. Finally, specific guideline and PIs developed by APPA which is globally well-known association in the field of FM for higher education facilities has been added to the combined PI which is the mixed form of FM_KS and PIs from the previous literature. This research provides campus facility managers and FM outsourcing service providers with framework in the form of PI to support future directions of effective campus FM services.

대식세포의 식세포활동과 화학주성에 대한 인삼분획물의 영향

신은경,김세창,Shin, Eun-Kyoung,Kim, Sei-Chang 배재대학교 자연과학연구소 1996 自然科學論文集 Vol.8 No.2

쥐의 복강과 폐포 대식세포를 분리하고 배양하여 효모에 대한 식세포활동 및 화학주성을 관찰하였다. 식세포활동이 일어나는 양상을 wright-Giemsa로 염색하여 사진으로 확인하였다. 복강 대식세포의 경우 diol saponin 처리군에 의해 약 48%까지 식세포활동이 증가되었고 total saponin 처리군에 의해 약 35%까지 식세포활동이 감소되었다. 폐포 대식세포의 경우 모든 인삼분획물 처리군에 의해 최고 50%까지 섭식이 증가되었다. 화학주성은 복강 대식세포의 경우 인삼분획물 처리군 모두 약 17%까지, 폐포 대식세포에서는 diol saponin만 약 16%까지 이동되었다. actin의 증감을 SDS-PAGE로 확인해 보았으나 변화가 없었다. The phagocytosis and chemotaxis of murine macrophages after treated with saponin fractions are investigated. Phagocytic appearance against yeast was photographed by dying with Wright-Giemsa. Phagocytic activity of peritoneal macrophage was invreased in diol saponin treatment by 48% and was decreased in total saponin treatment by 35%. The ingestion of alveolar macrophage was increased by 50% maximally. Peritoneal chemotactic activity was shown in 17% increases and only diol saponin had effect in alveolar macrophage by 16%. According to SDS-PAGE method the contents of actin did not show any alterations.

초경전 여아에서 운동의 기계적 스트레인과 칼슘섭취량에 따른 발꿈치뼈 골밀도의 분포

신은경,김기숙,김희영,이인숙,정효지,조성일,Shin, Eun-Kyung,Kim, Ki-Suk,Kim, Hee-Young,Lee, In-Sook,Joung, Hyo-Jee,Cho, Sung-Il 대한예방의학회 2005 예방의학회지 Vol.38 No.3

Objectives : The effects of exercise on bone density have been found to be inconsistent in previous studies. We conducted a cross-sectional study in premenarcheal girls to test two hypotheses to explain these inconsistencies. Firstly,'the intensity of mechanical strain, in terms of the ground reaction force(GRF), has more important effects on the bone mass at a weight-bearing site', and secondly, 'calcium intake modifies the bone response to exercise'. Methods : The areal bone mineral density was measured at the Os calcis, using peripheral dual energy X-ray absorptiometry, in 91 premenarcheal girls aged between 9 and 12 years. The intensity of mechanical strain of exercise was assessed by a self-report questionnaire and scored by the GRF as multiples of body weight, irrespective of the frequency and duration of exercise. The energy and calcium intake were calculated from the 24-hour dietary recall. An analysis of covariance(ANCOVA) was used to determine the interaction and main effects of exercise and calcium on the bone density, after adjusting for age, weight, height and energy intake. Results : The difference in the bone density between moderate and low impact exercise was more pronounced in the high than low calcium intake group. The bone density for moderate impact exercise and high calcium intake was significantly higher than that for low impact exercise (p=0.046) and low calcium intake, after adjusting for age, weight, height and energy intake. Conclusions : Our study suggests that the bone density at a weight-bearing site is positively related to the intensity of mechanical loading exercise, and the calcium intake may modify the bone response to exercise at the loaded site in premenarcheal girls.

Escherichia coli 리보핵산 내부분해효소 RNase E의 돌연변이체 선별 및 특성분석

신은경,고하영,김영민,주세진,이강석,Shin, Eun-Kyoung,Go, Ha-Young,Kim, Young-Min,Ju, Se-Jin,Lee, Kang-Seok 한국미생물학회 2007 미생물학회지 Vol.43 No.4

대장균의 필수적인 리보핵산 내부분해효소인 RNase E는세포내에서 여러 RNA의 분해와 가공과정에서 중요한 역할을 하며, 이 단백질의 효소활성부위를 포함하는 N-말단부위의 498 아미노산(N-Rne)만의 발현으로도 세포의 생장을 가능하게 한다. 이러한 RNase E의 특성을 활용하여 다양한 표현형을 가지는 N-Rne 돌연변이체들을 분리, 동정할 수 있는 효율적인 유전학적 시스템을 개발하였다. 이 시스템을 이용하여 얻어진 효소활성부위 돌연변이체들을 표현형으로 분류하여 분석한 결과, S1 도메인의 6번째 아미노산의 치환(I6T)을 가진 변이체는 야생형 N-Rne의 기능을 대체하지 못하였고, Small 도메인의 488번째 아미노산의 치환(R488C)을 가진 변이체는 야생형 N-Rne의 발현양보다 현저히 작게 발현시켜도 세포의 생장을 정상적으로 가능하게 하였다. 또한 DNase I 도메 인의 305번째 아미노산의 치환(N305D)을 가진 변이체는 야생형 N-Rne의 발현양보다 과발현시켰을 때만 세포의 생장을 가능하게 하였다. 각각의 아미노산 치환을 포함하는 N-Rne를 한정적으로 과발현시켰을 때의 ColEl-타입 플라스미드의 복제 수에 대한 영향을 측정한 결과, 돌연변이체 N-Rne의 세포생장에 대한 영향은 이 변이체들의 세포 내 효소활성 정도에 기인하는 것으로 밝혀졌다. 이러한 실험결과는 이 연구에서 개발한 유전학적 시스템을 이용하여 다양한 표현형을 가진 RNase E 변이체를 선별할 수 있으며, 이 변이체들의 특성을 분석함으로써 RNase E가 RNA의 안정성을 조절하는데 있어서 각각의 세부 도메인의 역할을 규명할 수 있으리라는 것을 시사한다. RNase E is an essential Escherichia coli endoribonuclease that plays a major role in the decay and processing of a large fraction of RNAs in the cell and expression of N-terminal domain consisted of 1-498 amino acids (N-Rne) is sufficient to support normal cellular growth. By utilizing these properties of RNase E, we developed a genetic system to screen for amino acid substitutions in the catalytic domain of the protein (N-Rne) that lead to various phenotypes. Using this system, we identified three kinds of mutants. A mutant N-Rne containing amino acid substitution in the S1 domain (I6T) of the protein was not able to support survival of E. coli cells, and another mutant N-Rne with amino acid substitution at the position 488 (R488C) in the small domain enabled N-Rne to have an elevated ribonucleolytic activity, while amino acid substitution in the DNase I domain (N305D) only enabled N-Rne to support survival of E. roli cells when the mutant N-Rne was over-expressed. Analysis of copy number of ColEl-type plasmid revealed that effects of amino acid substitution on the ability of N-Rne to support cellular growth stemmed from their differential effects on the ribonucleolytic activity of N-Rne in the cell. These results imply that the genetic system developed in this study can be used to isolate mutant RNase E with various phenotypes, which would help to unveil a functional role of each subdomain of the protein in the regulation of RNA stability in E. coli.

대식세포의 화학주성과 세포내 칼슘과 Actin의 증가에 미치는 인삼사포닌 성분의 영향

신은경,김세창,Shin, Eun-Kyoung,Kim, Sei-Chang 배재대학교 자연과학연구소 1998 自然科學論文集 Vol.10 No.1

본 실험은 대식세포 화학주성과 세포내 칼슘과 F-actin 증가에 대한 인삼사포닌 분획의 영향을 알아보기 위하여 수행되었다. 여러 가지 인삼사포닌 분획을 처리한 복강 대식세포는 대조군에 비해 화학주성이 28.4-71% 증가하였다. 세포내에 유리된 칼슘의 양은 65%까지 증가하였으며, NBD- phallacidin을 처리한 세포에서 F-actin의 양은 10% 증가하였다. 칼슘이나 PMA로 활성화시키고 사포닌 분획을 처리하였을 때, F-actin의 양은 현저하게 증가하였으며 이러한 현상은 2분까지 지속되었다. 이러한 결과로 보아 인삼사포닌 분획이 chemoattractant로 작용할 수 있을 것으로 생각된다. In the present study, We have tested the potential effects of ginseng saponin fractions on macrophage chemotaxis and intracellular calcium and F-actin mobilization. Peritoneal macrophages treated with various ginseng saponin fractions showed 28.4% to 71% of increasement of chemotaxis as compared with untreated cells. The activity of intracelluar calcium mobilization was increased up to 65% by treatment with saponins, and F-actin content also increased 10% in the cells loaded with NBD-phallacidin. When the cells were activated with calcium of PMA and treated with saponin fractions, the intracelluar F-actin content increased significantly and prolonged for 2 minutes. These results suggest that ginseng saponin fractions might be a chemoattractants.

마우스에서 Capillary hepatica 감염에 의한 간섬유증의 병리학적 연구

신은경,한정희,Shin, Eun-kyung,Han, Jeong-hee 대한수의학회 1998 大韓獸醫學會誌 Vol.38 No.1

This study was performed to investigate the pathogenesis of hepatic granuloma and hepatic fibrosis induced in mice infected with Capillaria(C) hepatica and treated cyclophosphamide. The results were as grossly well-defined yellowish white spots and small nodules at the surface of the liver were scattered. Histopathologically, there were numerous granulomas composed of eggs and fragments of C hepatica surrounded by heavy infiltration of inflammatory cells. Severe fibrosis was observed around granulomas. Pathological lesions of group infected with C. hepatica and then injected with cyclophosphamide were most severe than those of other groups. Therefore this study suggested that hepatic fibrosis induced by C hepatica in mice would be useful for animal model of hepatic fibrosis in human.

단기간 영양교육 캠프가 비만아동의 식품과 영양소 섭취에 미치는 영향

신은경(Eun-Kyung Shin),이진화(Jin-Hwa Lee),이연경(Yeon-Kyung Lee),이혜성(Hye-Sung Lee) 한국식품영양과학회 2005 한국식품영양과학회지 Vol.34 No.7

본 연구는 단기간의 영양교육캠프가 비만아동의 식품과 영양소 섭취에 미치는 영향을 조사하고자 하였다. 연구대상은 경상북도 구미시에 거주하는 비만도 130%이상 비만아동 30명이었으며, 이들과 학부모를 대상으로 여름과 겨울방학을 이용하여 2회의 영양교육 캠프를 실시하였다. 식품 및 영양소 섭취상태는 영양교육 캠프 전ㆍ중ㆍ후의 3회에 걸쳐 3일간 식품기록법을 이용하여 조사되었다. 평균연령은 남녀 각각 10.2, 10.0세였다. 평균 체중은 남녀 각각 51.0±9.1 ㎏, 52.7±10.4 ㎏이었고, 평균 신장은 남녀 각각 144.7±6.8 ㎝, 142.2±8.7 ㎝였으며, BMI는 남아가 24.3±4.5, 여아가 26.2±5.4였다. 일일 평균 식품 총섭취량은 캠프 전 1646.64±1403.6 g, 캠프 중 1438.6±1130.6 g, 캠프 후 1412.66±881.43g으로 차츰 감소하였다. 이중에서 식물성 식품군으로부터 74.6~66.7%를 섭취하였고, 동물성 식품군으로부터 25.4~33.3%를 섭취하였다. 섭취량이 가장 높았던 식품군은 곡류였으며, 그 다음이 과실류, 우유류, 채소류, 음료류, 육류, 난류, 두류 순이었다. 곡류, 두류의 섭취량은 영양교육 캠프전에 비해 캠프 중과 후에 유의하게 낮았고, 난류의 섭취는 캠프 중에 감소하였다가 캠프 후에 다시 증가하였으며 어패류의 섭취량은 캠프 중과 후에 유의하게 높아졌다. 영양교육 캠프 후 섭취량이 유의하게 변화한 것은 에너지와 당질 및 비타민 C였다. 에너지 섭취량은 캠프 전에 RDA의 105.9%에서 캠프 후에 RDA의 91.6%로서 유의하게 낮아졌으며 당질의 섭취량도 캠프 전(323.02±236.83 g/d)에 비해 캠프 후(282.57±143.41 g/d)에 유의하게 감소하였다. 비타민 C의 섭취는 캠프 전(108%RDA)에 비해 캠프 후(166%RDA)에 유의하게 증가하였다. 비만아동들이 RDA에 비해 낮게 섭취한 영양소는 칼슘과 비타민 B2로 나타났다. 3대 열량 영양소로부터의 에너지 섭취비율에서 지방 에너지 섭취율은 경우기간에 따라 유의한 차이가 없었으나, 단백질 에너지 섭취율은 영양교육 캠프 후 유의하게 높아졌고, 당질 에너지 비율은 유의하게 낮아졌다. 동물성과 식물성 급원별 일일 주요영양소(단백질, 지방, 칼슘, 철) 섭취량은 영양교육 캠프의 영향을 받지 않았다. 칼슘은 동물성ㆍ식물성 급원의 섭취 비율이 60:40, 철분은 20:80으로 나타났다. 곡류와 우유류의 에너지 섭취율은 캠프 전에 비해 캠프 중과 후에 유의하게 감소하였고, 난류의 에너지 섭취율은 캠프 중에 유의하게 낮았다. 단백질의 경우 곡류와 감자류, 채소류, 어패류로부터의 섭취율이 캠프 후에 유의하게 증가하였고, 지방은 곡류, 우유류로부터의 섭취율이 캠프 후 유의하게 감소하였다. 칼슘의 경우 감자류와 우유류로부터의 섭취율이 캠프 중과 후에 유의하게 증가하였고, 난류로부터 유의하게 감소하였다. 철의 경우 감자류와 어패류로부터 섭취율이 캠프 중과 후에 유의하게 증가하였고, 곡류로부터의 섭취율이 캠프 중에 유의하게 낮았다. 비타민 A의 경우 해조류와 난류로부터의 섭취율이 캠프 후에 유의하게 증가하였고, 채소류로부터의 섭취율이 캠프 중에 유의하게 높았고 비타민 B₁은 채소류와 어패류로부터의 섭취율이 캠프 중에 유의하게 높은 것으로 나타났다. 조섬유의 경우 곡류로부터 얻은 양이 캠프 중과 후에 유의하게 감소하였으며, 콜레스테롤의 경우 난류로부터 얻은 양이 캠프 중에 유의하게 낮았다. 이상의 결과로부터 2주일간의 하계영양교육캠프와 3일간의 동계영양교육캠프 후 비만아동들은 식품 총섭취량과 곡류식품의 유의한 감소와 어패류 섭취량의 유의한 증가를 보였다. 영양소 섭취면에서는 열량과 탄수화물 섭취량의 유의한 감소와 단백질과 비타민 C 섭취량의 유의한 증가와 칼슘과 철분의 섭취 증가 양상을 보였다. 열량섭취에서는 당질 에너지 섭취율의 감소와 단백질 에너지 섭취율의 증가를 보였다. 따라서 영양교육 캠프의 실시가 비만아동들의 전반적인 식품과 영양소 섭취상태의 변화에 긍정적인 효과를 미친 것으로 나타났다. The purpose of this study was to evaluate the effect of short-term nutrition education camp on food and nutrient intakes of obese children. The subjects of the study were 30 obese children in Gumi city who have obesity index over 130%. The food consumption of the subjects was surveyed for three days before the camp, for eleven days during the first camp and for three days after the second camp, by food record method. The mean intakes of energy and carbohydrates in obese children were significantly decreased during and after the nutrition education camp, and that of vitamin C was significantly increased after the camp. The mean daily total food intake of the subjects was gradually decreased from 1646.4 g before the camp to 1438.6 g during the camp and 1412.66 g after the camp. Energy and fat intake ratios from grains and milks were significantly decreased after the camp compared with that before the camp, and the energy intake ratio from grains, milks and eggs was significantly low during the camp. The protein intake ratios from grains, potatoes, vegetables and fishes were significantly increased after the camp. The calcium intake ratios from potatoes and milks were significantly increased during and after the camp, and the ratio from eggs was significantly decreased after the camp. The iron intake ratios from potatoes and fishes were significantly increased during and after the camp, and the ratio from grains was significantly low during the camp. The results of the study demonstrated that the short-term nutrition education program positively affected the overall food consumption and nutrients intakes of obese children.

한 · 난 배색이미지의 연령별 색채감성 평가에 관한 연구

신은경(Shin, Eun-Gyeong),박수빈(Park, Soobeen) 대한건축학회 2017 대한건축학회논문집 Vol.33 No.10

This study compared emotional responses to color combinations in the 60s group to those in the younger groups(20s, 40s) in order to develop simple and accurate measuring tools. With increasing elderly population, aging vision is a factor that considerably impacts color visualization. The environment surrounding us stimulates our vision not only through visualizing one color separately but also through visualizing color combinations composed of more than two colors. This study focused on subjects’ emotional responses to a long wavelength color (R) and short wavelength color (PB) combinations. Twelve living room images were constructed using a computer graphic program. The rooms were two monochromatic color combinations, two analogous color combinations and two complementary color combinations in 200 lx and 500 lx. The images were presented through a 23-inch LED monitor. The subjects responded to the sensitivity vocabulary by semantic differential (SD) scales, while watching the images in a room with the natural light blocked. A total of 90 questionnaires were analyzed by SPSS 18.0. Major findings as follows: 1) When comparing each of the color images by SD scale responses, 60s showed significant differences compared to 20s and 40s in color emotion. 2) When comparing in 500 lx color combination images, there was no significant differences between each group. This study found the characteristics of people’s emotional responses to color combinations. This study will provide one basic data on color psychology research and directions of color design by analyzing the color emotion difference of color combinations according to age.

『往五天竺國傳』에 대한 비교문학적 연구

辛恩卿(Shin Eunkyung) 한국언어문학회 2008 한국언어문학 Vol.66 No.-

  This article intends to illuminate the characteristics and significance of the Wangochonchukkukchon, the records of experiences of travel to Chonchuk --the old name of India-- by Hyecho(704-780), a Buddhist monk of Silla. As Hyecho"s travel to India and its records go, there are two notable points which will be focused on in this article. One is that Hyecho"s travel was done at the age of twenty which was young age compared to other monks" cases, and the other is that the Wangochonchukkukchon is differentiated from other travel literatures by Buddhists monks in including some poems within the text.<BR>  Unlike other studies on the Wangochonchukkukchon which have mainly been established around bibliographical approach, this article is distinctive in employing a comparative method which covers other similar texts by Buddhist monks such as the Pulgukki by Peophyun, the Taedang seoyuk kubeop koseungchon and the Namhaegigwinae peopchon by Uijeong, the Taedang seoyukki by Hyunjang, and the Iptang kubeop sunnye hanggi by Japanese monk Yennin.<BR>  When we classify travel literature, according to the purpose of the travel such as pilgrimage, sightseeing, and business trip, the Wangochonchukkukchon falls into the category of pilgrimage travel literature. Since Chonchuk was conceived of to be the sacred place by Buddhists which Buddha had been born, achieved Enlightenment, and preached on the four holy truth--birth, aging, illness, death--, it can be characterized by a form of pilgrimage to visit holy places and worship the Buddhist relics in India, It, however, was a risky attempt fraught with a quantity of difficulties and dangers to make a trip to India in the 8th century. The experience of pilgrimage to India, which signified an accomplishment of a holy mission, brought a tremendous changes to Hyecho both in inner and in outer life. On one hand he became to form his self identity as a real Buddhist monk, and on the other hand he could consolidate his religious standing in the Tang period. In other words, it can be said that the pilgrimage to India functioned the rite of passage to him.<BR>  As for the writing style of the Wangochonchukkukchon, it can be categorized as ‘poem-inserted type’ among various types of the mixed-style texts: those which prose and verse are mixed in a text. This article considers this characteristic of the Wangochonchukkukchon to be one influenced by Uijeong"s travel literature.