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당귀용회환(當歸龍?丸)의 glutamate에 의한 청신경세포(聽神經細胞) 손상(損傷) 보호효과(保護效果)
유동희,박래길,소홍섭,이기남,정명수,Yu, Dong-Hee,Park, Rae-Gil,So, Hong-Seob,Lee, Ki-Nam,Chong, Myong-Soo 대한예방한의학회 2012 대한예방한의학회지 Vol.16 No.2
Objective : The water extract of Danguiyonghoihwan (DGYHW) has been traditionally used in treatment of tinnitus in Oriental Medicine. However, little is known about the mechanism by which DGYHW rescues auditory neuronal cells from injury damages. Therefore, in this study I effort to elucidate the mechanism of the cytoprotective effect of the DGYHW extract on glutamate-induced auditory sensorineuronal cell death. Methods : I determined the elevated cell viability by DGYHW extract on glutamate-induced auditory sensorineuronal cell death. Glutamate induced neuronal damage in oranotypic explant culture also, glutamate decreased cell viability on VOT-33 cells but pretreatment with DGYHW inhibited cell death. Results : One of the main mediator of glutamate-induced cytotoxicity was known to generation of reactive oxygen species (ROS). Pretreatment with DGYHW inhibited this ROS generation from glutamated-stimulated VOT-33 cells. Also, I identified that the ROS-induced DCF-DA green fluorescence is reduced by DGYHW pretreatment. The critical markers of apoptotic cell death were cleavages of procaspase-3 protease protein. So I checked the expression level and cleavage of procaspase-3 protease protein. Glutamate-treated VOT-33 cells were shown to have cleavage of procaspase-3 protease proteins and following reduction of expression of these proteins. But I found that pre-treatment with DGYHW protects glutamate-induced changes of biochemical marker protein, caspase-3. Conclusion : These findings indicated that DGYHW may prevent cell death from glutamate induced VOT-33 cell death by inhibiting the ROS generation and modulation of protein expressions in procaspase-3, catalase and Bcl-2.
12주간의 항산화제 복합투여가 VO2max의 변화 및 항산화 관련 유전자 발현에 미치는 효과
정원훈 ( Won Hun Jeong ),소홍섭 ( Hong Seob So ),박찬희 ( Chn Ny Park ),김용규 ( Yong Kyu Kim ),한상완 ( Sang Wan Han ),은희관 ( Hee Gowan Eun ),송제호 ( Je Ho Song ),박래길 ( Rae Kil Park ) 한국운동영양학회 2004 Physical Activity and Nutrition (Phys Act Nutr) Vol.8 No.2
This study was to investigate the effect of antioxidant supplements, vitamin C and E on VO2max and anti-oxidant related gene expressions in college handball players. Fourteen subjects were divided into two groups, the antixoidant supplement group (ASG) and the placebo supplement group (PSG). The ASG group had 12 weeks of vitamin C and E supplementations with high intensity training, while the PSG group had placebo tablets with the same training intensity and duration that the ASG group did. The VO,max values after 12 weeks in the ASG and PSG groups were 64.43 ± 1.27 ml/kg/min and 62.14 ± 1.35 ml/kg/min, respectively. The ASG group compared to the PSG group had significantly higher VO₂max (p<0.01). The RT-PCR analysis data showed that the PSG group compared to the ASG group had significantly higher mRNA. expression levels of the anti-oxidant related genes such as SOD-1, GPx-4, GSSG-R, CAT and GSH-S at sixth week. Taken together, these results suggest that antixoidant supplements such as vitamin C and E may have some beneficial effects to enhance endurance capacity through the up-regulation of the antioxidant system.
Sucrose 발효 및 비발효 Vibrio vulnificus 균주의 생물학적 및 유전학적 성상 비교
김신무(Shin-Moo Kim),소향아(Hyang-Ah So),송계민(Kye-Min Song),이영엽(Young-Youp Lee),임채원(Chae-Won Lim),이재형(Jae-Hyung Lee),소홍섭(Hong-Seob So),김진경(Jin-Kyung Kim),박래길(Rae-kil Park),박석돈(Seok-Don Park) 대한미생물학회 2006 Journal of Bacteriology and Virology Vol.36 No.4
Gliotoxin에 의해 유발된 아폽토시스에 대한 Protein Kinase C (PKC) 전처리의 영향
허정무(Jung-Mu Her),오재민(Jay-Min Ohl),박래길(Rae-Kil Park),소홍섭(Hong-Seob SO),문연자(Yeun-Ja Mun),최민규(Min-Kyu Chai),이갑상(Gab-Sang Lee),정연태(Yeun-Tai Chung),박옥규(Ock-Kyu Park) 대한체질인류학회 2000 해부·생물인류학 (Anat Biol Anthropol) Vol.13 No.1
Gliotoxin은 Penicillium, Aspergillus, Gliocladium 및 Thermoascus와 같은 곰팡이들로부터 생성되는 대사산물중 하나로, 공통적으로 epipolythiodioxopiperazine (ETP)계 구조를 가지고 있다. 이 ETP계 구조에 의해 질병이나 면역조정기능과 같은 다양한 생리적 효과를 나타낸다. 심근세포인 H9c2세포에 gliotoxin을 처리한 결과 caspase-3의 활성에 의해 DNA fragmentation 을 유발시켜 세포의 손상을 유도하였다 Phorboll2-myristate 13-acetate(PMA)를 시간별로 처리한 경과, gliotoxin을 처리하기 전에 PMA를 15분전에 처리한 경우에는 caspase-3의 활성을 억제시켜 세포의 손상을 억제시켰으나, PMA를 gliotoxin과 동시에 처리하거나 후처리한 경우에서는 세포의 손상을 억제시키지 못했다. 따라서 ,PMA 천처리에 의해서 protein kinase C (PKC)가 활성화되며, 이 활성화된 PKC가 caspas-3의 활성을 차단하므로서, 아폽토시스를 억제한다는 것을 알 수 있었다.
당지질로 유도한 염증반응에서 Piceatannol의 항염증 기전 연구
조한진(Han Jin Cho),심재훈(Jae-Hoon Shim),소홍섭(Hong-Seob So),윤정한(Jung Han Yoon) 한국식품영양과학회 2012 한국식품영양과학회지 Vol.41 No.9
본 연구에서는 염증반응을 조절하는 다양한 신호전달체계를 중심으로 분자생물학적 방법을 통해 piceatannol의 항염증 기전을 규명하였다. LPS로 염증반응을 유도한 Raw 264.7 대식세포에서 piceatannol은 iNOS의 발현 억제를 통해 NO의 생성을 감소시키고 염증성 사이토카인(TNF-α, IL-6, IL-1β)의 생성을 감소시켰다. 염증반응을 조절하는 신호전달체계 중 piceatannol은 LPS에 의해 유도된 IκB의 분해와 p65의 핵으로의 이동을 억제하고, LPS에 의해 유도된 SAPK/JNK의 인산화를 억제하였다. 또한 piceatannol은 LPS와 IL-6(LPS에 의해 증가됨)에 의한 STAT3의 활성화를 억제하였다. 뿐만 아니라 piceatannol은 Nrf2의 핵 내 축적을 야기하고 ARE의 transcriptional activity를 증가시켜 HO-1의 발현을 증가시켰다. 본 연구의 결과, piceatannol은 NF-κB와 AP-1, STAT3 신호전달의 억제를 통해, 그리고 HO-1의 발현 증가를 통해 항염증 효과를 나타내었다(Fig. 8). 3,4,3",5"-Tetrahydroxy-trans-stilbene (piceatannol) is a derivative of resveratrol with a variety of biological activities, including anti-inflammatory, anti-proliferative, and anti-cancer activities. We assessed the mechanisms by which piceatannol inhibits inflammatory responses using lipopolysaccharide (LPS)-treated Raw264.7 murine macrophages. Piceatannol (0~10 μmol/L) decreased LPS-induced release of nitric oxide, tumor necrosis factor (TNF)-α, interleukin (IL)-6, IL-1β, and inhibited LPS-induced protein expression of inducible nitric oxide synthase (iNOS). Activation of nuclear factor-kappaB (NF-κB), activator protein (AP)-1, and signal transducer and activator of transcription 3 (STAT3) are crucial steps during an inflammatory response. Piceatannol prevented LPS-induced degradation of inhibitor of κB (IκB), translocation of p65 to the nucleus, and phosphorylation of stress-activated protein kinase/c-Jun NH2-terminal kinase (SAPK/JNK). Additionally, piceatannol inhibited LPS-induced phosphorylation of STAT3 and IL-6-induced translocation of STAT3 to the nucleus. Furthermore, piceatannol increased the protein and mRNA levels of hemeoxygenase (HO)-1, the rate-limiting enzyme of heme catabolism that plays a critical role in mediating antioxidant and anti-inflammatory effects. Piceatannol further induced antioxidant response elements (ARE)-driven luciferase activity in Raw264.7 cells transfected with an ARE-luciferase reporter construct containing the enhancer 2 and minimal promoter region of HO-1. These results suggest that piceatannol exerts anti-inflammatory effects via the down-regulation of iNOS expression and up-regulation of HO-1 expression.