수입 냉동새우에서 검출된 YHV3와 IHHNV의 계통학 및 병원성 분석

백은진,정예진,정민아,박지연,김광일,Baek, Eun Jin,Joeng, Ye Jin,Jeong, Min A,Park, Ji Yeon,Kim, Kwang Il 한국어병학회 2022 한국어병학회지 Vol.35 No.1

Yellow head virus (YHV), Infectious hypodermal and hematopoietic necrosis (IHHNV), Taura syndrome virus (TSV), and Infectious myositis virus (IMNV) cause serious mortality to Penaeidae shrimp in the aquaculture. In this study, YHV, IHHNV, TSV, and IMNV were surveyed from imported frozen shrimps between 2019 and 2020 via molecular diagnostic assay. Among 10 shrimp groups, YHV (n=1) and IHHNV (n=4) were detected by RT-PCR and PCR, respectively. From the phylogenetic analysis based on the partial ORF 1b region of YHV, YHV was classified into YHV genotype 3 (YHV3). And IHHNVs (n=2) detected from Litopenaeus vannamei belong to infectious IHHNV type 2. Although IHHNVs (n=2) identified from Penaeus monodon showed PCR positive results (MG 831F/R primer set), the sequences of ORF 2 and 3 were not amplified, suggesting that those samples might possess type A IHHNV related sequence of P. monodon. Furthermore, in the challenge test, even though PCR-detected isolates (YHV3/type A IHHNV related sequence or infectious IHHNV type 2) were not induced mortality to L. vannamei, viral genes were amplified suggesting that the viruses in the frozen shrimp could be non-pathogenic particles which are not enough to induce mortality.

역사학습의 목적과 역사교사의 역사교육 목적에 대한 중고등학생들의 인식

白銀珍(Baek, Eun-Jin) 歷史敎育硏究會 2015 역사교육 Vol.133 No.-

There are two main agents in history classes: teacher and students. This research is about the students’ perception, especially perception of teacher’s purpose of the teaching history. The research is carried out by in-depth interviews with students. Students are also asked to say their own understanding on the purpose of learning history, in order to discover how they find a meaning in their history classes. 15 students are interviewed; 6 of them are middle school students, and the others are high school students in Seoul or Gyeonggi-do Province. The following are the observations drawn from this research. First, students’ understanding of learning history is more likely based on personal context or experience than that of teachers. Second, even though some students have partial understanding of their teacher’s purpose, this doesn’t always mean they sympathize their teacher’s view. Third, with their own basis, some students hold different views on the goals of teaching history set by their teacher, or on the content or teaching methods based on them. Fourth, no correlation is found between their understanding of the teachers’ objective and their study achievement. Understanding these purposes set by students and their perception of their teacher’s purpose will be helpful to building more communicative history class.

Differences of Growth Characteristics in Turmeric Cultivated Using Mother and Finger Seed Rhizomes

Eun Jin Baek(백은진),Hee Jeong Jeong(정희정),Seok Ha Yoon(윤석하),Kwan Su Kim(김관수) 한국약용작물학회 2017 한국약용작물학회 학술대회논문집 Vol.2017 No.2

역사교사의 역사교육 목적에 관한 사례 연구

白銀珍(Baek, Eun-Jin) 역사교육연구회 2014 역사교육 Vol.131 No.-

The discourse about the purpose of the history education has rarely been seen since Independence of Korea in 1945. It is partly because the top-down approach, which is divorced from the actual classroom experience. Also, there has rarely been research on what teachers and students actually consider as the purpose of history education and what they really teach and learn. It is a natural consequence of the stance that regards teachers as neutral deliverer of historical facts. Defying this trend, this article deals with the purposes of teaching history actually set by teacher, who is the primary agents of the education, and the practices to achieve them. A history teacher in middle school was selected for this research. In depth interviews on his perceptions on purposes of teaching history were conducted, and their classroom practices were observed to find realizations or distortions of their expectations. His views are commonly based on the belief that the history education would be beneficial to real life of the students. And he addressed the purposes of his teaching in the test class. He included all units of the text book in their annual curriculum, and continued to apply and use single method and text book. Therefore, history teachers’ education institutes should provide programs which allow history teachers to make a choice of the contents and methods of teaching that suit their own intention. Through this, they should learn how to reflect their intention to their teaching and how to self-examine it.

올리브유의 잔류농약 모니터링 및 노출량 조사

손미희,김재관,이유진,김지은,백은진,김병태,이성남,박명기,박용배,Mi-Hui Son,Jae-Kwan Kim,You-Jin Lee,Ji-Eun Kim,Eun-Jin Baek,Byeong-Tae Kim,Seong-Nam Lee,Myoung-Ki Park,Yong-Bae Park 한국식품위생안전성학회 2023 한국식품위생안전성학회지 Vol.38 No.4

A total of 100 commercially available olive oil products were analyzed for 179 pesticide residues using gas chromatography-tandem mass spectrometry (GC/MS/MS). The olive oil samples were mixed with organic solvents, centrifuged and frozen to remove fat, and pesticide residues were analyzed using the "quick, easy, cheap, effective, rugged, and safe" (QuEChERS) method. The determination coefficient (R²) of the analysis method used in this study was ≥0.998. The detection limit of the method ranged 0.004-0.006 mg/kg and its quantitative limit ranged 0.012-0.017 mg/kg. The recovery rate (n=5) measured at the level ranging 0.01-0.02, 0.1, and 0.5 mg/kg ranged 66.8-119.5%. The relative standard deviation (RSD) was determined to be ≤5.7%, confirming that this method was suitable for the "Guidelines for Standard Procedures for Preparing Food Test Methods". The results showed that a total of 151 pesticides (including difenoconazole, deltamethrin, oxyfluorfen, kresoxim-methyl, phosmet, pyrimethanil, tebuconazole, and trifloxystrobin) were detected in 64 of the 100 olive oil products. The detection range of these pesticide residues was 0.01-0.30 mg/kg. The percentage acceptable daily intake (%ADI) of the pesticides calculated using ADI and estimated daily intake (EDI) was 0.0001-0.1346, indicating that the detected pesticides were present at safe levels. This study provides basic data for securing the safety of olive oil products by monitoring pesticide residues in commercially available oilve oil products. Collectively, the analysis method used in this study can be used as a method to analyze residual pesticides in edible oils.

경기서부지역 로컬푸드 잔류농약 실태조사

손미희,김재관,이유진,김지은,백은진,김병태,이성남,박명기,박용배,Mi-Hui Son,Jae-Kwan Kim,You-Jin Lee,Ji-Eun Kim,Eun-Jin Baek,Byeong-Tae Kim,Seong-Nam Lee,Myoung-Ki Park,Yong-Bae Park 한국식품위생안전성학회 2023 한국식품위생안전성학회지 Vol.38 No.6

경기 서부지역 로컬푸드 직매장에서 유통되고 있는 농산물 341건을 수거하여 잔류 농약 검사를 실시한 결과 30.8%인 105건에서 잔류농약이 검출되었다. 6건이 잔류허용기준을 초과하여 부적합률은 1.8%로 나타났으며 최근 3년간 평균 부적합률인 1.4%보다 다소 높게 나타났다. 조사대상 농산물 중 과일류와 채소류에서만 잔류농약이 검출된 것으로 나타났으며, 과일류는 34건 중 24건이 검출되어 70.6%, 채소류는 277건중 81건이 검출되어 29.2%의 검출률을 나타내었다. 검출된 농약은 acetamiprid 등 59종으로 총 208회 검출되었으며 검출범위는 0.01-2.38 mg/kg 이었다. 잔류농약이 검출된 105건의 농산물중 62건(59%)에서는 1종의 농약만 검출되었고 43건(41%)에서는 2종 이상의 농약성분이 검출되었다. 특히 복숭아에서는 14종의 농약이 동시에 검출되었으며 농약성분 중 dinotefuran이 21회로 가장 많이 검출되었다. 검출된 농약의 독성을 살펴본 결과 인축독성은 III급(보통독성)이 44회(21.2%), IV급(저독성)이 164회(78.8%) 검출되었고, 어독성은 I급 68회(32.7%), II급 14회(6.7%), III급 126회(60.6%) 검출된 것으로 나타났다. 5회 이상 검출된 다빈도 농약성분에 대한 노출량을 조사한 결과 위해지수는 2.8% 이하로 나타나 식이 섭취량에 따른 잔류농약의 위해도는 미미한 것으로 판단된다. In this study, we detected the presence of residual pesticides in 341 agricultural products collected from local food outlets in western Gyeonggi Province. Residual pesticides were detected in 105 (30.8%) samples. Six samples exceeded the legal limits for residual pesticides, resulting in a non-compliance rate of 1.8%, which was slightly higher than the average non-compliance rate of 1.4% in the last three years. Among the tested agricultural products, only fruits and vegetables were found to have pesticide residues, with 24 of 34 fruits (a detection rate of 70.6%) and 81 of 277 vegetables (a detection rate of 29.2%) testing positive. In total, 59 types of pesticides, including acetamiprid, which was detected 208 times, were detected and had a detection range of 0.01-2.38 mg/kg. Among the 105 agricultural products containing pesticide residues, a single pesticide was detected in 62 samples (59%) and two or more pesticides were detected in 43 samples (41%). In particular, 14 pesticides were detected in the same sample of peaches; dinotefuran was detected 21 times. Upon examining the toxicity of the detected pesticides, Class III pesticides (moderate toxicity) were detected 44 times (21.2%) and Class IV pesticides (low toxicity) were detected 164 times (78.8%). Class I, II, and III pesticides with fish toxicity were detected 68 (32.7%), 14 (6.7%), and 126 times (60.6%), respectively. Upon examining the exposure to high-frequency pesticide components detected five or more times, the hazard index was found to be ≤2.8%. Accordingly, the hazard of residual pesticides based on dietary intake was deemed insignificant.

고추씨 기름의 잔류농약 모니터링

손미희,김재관,이유진,김지은,백은진,김병태,박명기,박용배,Mi-Hui Son,Jae-Kwan Kim,You-Jin Lee,Ji-Eun Kim,Eun-Jin Baek,Byeong-Tae Kim,Myoung-Ki Park,Yong-Bae Park 한국식품위생안전성학회 2023 한국식품위생안전성학회지 Vol.38 No.6

2022년 8월부터 12월까지 시중에서 유통되는 고추씨기름 4건과 고추맛기름 36건에 대한 잔류농약 실태를 조사 하였다. 총 40건의 시료에 대해 179종의 농약을 모니터링한 결과 39건에서 14종의 농약이 검출되었으며 검출범위는 0.01-2.16 mg/kg이었다. 고추씨기름에선 10종의 농약이 27회 검출되었고 검출범위는 0.02-2.16 mg/kg 이었으며 고추맛기름은 9종의 농약이 94회 검출되었고 검출범위는 0.01-0.80 mg/kg 이었다. 가장 빈번하게 검출된 농약은 tebuconazole, ethion, difenoconazole이었으며 중국산 원재료를 사용한 제품에서 ethion이 다수 검출되었다. 국내 미등록 농약인 ethion은 경기도에서 최근 10년간 검출된 적이 없는 농약성분으로, ethion이 검출시 중국산 제품으로 판단할수 있는 지표물질로 활용이 가능할 것으로 사료된다. 고추는 농약이 많이 사용되는 대표적인 농산물 중 하나로, 고추씨로 전이된 농약이 제거되지 않을 경우 고추씨기름에서 다양한 종류의 농약이 검출될 확률이 매우 높다. 따라서 고추씨기름의 안전성을 보장하기 위해선 지속적인 연구조사가 필요할 것으로 사료된다. The status of residual pesticides was investigated in four pepper seed oil samples and 36 pepper-flavored oil samples oil distributed on the market from August to December 2022. A total of 179 pesticides were monitored in 40 samples, and 14 pesticides were detected in 39 of the samples, with a detection range of 0.01-2.16 mg/kg. In chili seed oil, 10 pesticides were detected 27 times with a range of 0.11-2.16 mg/kg, and in pepper-flavored oil, 9 pesticides were detected 94 times with a range of 0.01-0.80 mg/kg. The most frequently detected pesticides were tebuconazole, ethion, and difenoconazole, with ethion being detected in large concentrations in products using Chinese raw materials. Ethion, an unregistered pesticide in the Republic of Korea, has not been detected in the Gyeonggi-do area in the past 10 years. It is thought that the detection of ethion can be utilized as an indicator of products made in China. Peppers are a representative agricultural product for which many pesticides are used, and if the pesticides transferred to pepper seeds are not removed, the probability of detecting various types of pesticides in pepper seed oil is very high. Therefore, continuous research is needed to ensure the safety of pepper seed oil.

경기도 내 판매되고 있는 포도씨유의 잔류농약 모니터링

손미희,김재관,이유진,김지은,백은진,김병태,박명기,권보연,Mi-Hui Son,Jae-Kwan Kim,You-Jin Lee,Ji-Eun Kim,Eun-Jin Baek,Byeong-Tae Kim,Myoung-Ki Park,Bo-yeon Kwon 한국식품위생안전성학회 2024 한국식품위생안전성학회지 Vol.39 No.2

경기도에서 유통중인 포도씨유 50건을 대상으로 잔류농약 실태를 조사하였다. 50건 중 49건에서 10종의 잔류농약이 161회 검출되었다. 검출된 농약은 boscalid, cyflufenamid, deltamethrin, difenoconazole, fluxapyroxad, fenpyrazamine, kresoxim-methyl, piperonyl butoxide, tebuconazole, trifloxystrobin으로 살충제 2종, 살균제 8종이었으며 살균제인 boscalid가 44회, fluxapyroxad가 35회로 가장 빈번하게 검출되었다. 검출범위는 0.01-1.10 mg/kg으로 모두 포도의 잔류농약 허용 기준 이내로 검출되었다. 검출된 농약의 회수율은 72.6-129.8%이었으며 검출된 농약의 위해성을 알아보기 위해 %ADI를 산출한 결과 0.0028% 이하로 나타나 포도씨유의 잔류농약으로 인한 위해성은 안전한 수준으로 조사되었다. 그러나 본 연구는 시료의 수가 적고 GC/MS/MS로 분석가능한 농약만 조사하였기 때문에 보다 신뢰성 있는 결과를 얻기 위해선 앞으로도 꾸준한 추적 조사가 필요할 것으로 사료된다. Using the freezing removal method, we investigated residual pesticides in 50 grape seed oils distributed in Gyeonggi Province, South Korea. The fat was mixed with acetonitrile and then frozen at ≤-20℃ for 24 h. Fats and oils were removed by separating those in solid state and the extract acetonitrile in liquid state. Ten residual pesticides were detected 161 times in 49 of 50 cases. The detected pesticides were boscalid, cyclufenamide, deltamethrin, difenoconazole, fluxapyroxad, fenpyrazamine, kresoxim-methyl, piperonyl butoxide, tebuconazole, and trifluoxysorbin. Boscalid, a fungicide, was most frequently detected (44 times), followed by fluxapiroxad (35 times). The detection range was 0.01-1.10 mg/kg, which was within the legal limit of residual pesticide for grapes. The recovery rate of the detected pesticides was 72.6-129.8% and the ratio of estimated daily intake/acceptable daily intake was calculated to determine the risk of the detected pesticides, which was <0.0028%. This indicated that the risk caused by pesticide residues in grape seed oil is at a safe level.

효율적인 Regular Path Query 처리에 관한 연구 조사

이태성 ( Taesunq Lee ),백은진 ( Eun-jin Baek ),황준승 ( Junseunq Hwanq ),김경민 ( Kyounqmin Kim ),한욱신 ( Wook-shin Han ) 한국정보처리학회 2017 한국정보처리학회 학술대회논문집 Vol.24 No.1

본 연구는 regular path query를 효율적으로 처리하는 디스크 기반 시스템을 만들기 위해서는 그래프 데이터를 효율적으로 저장하여야 하며, regular path query 수행 시 발생하는 cost 가 작도록 하는 evaluation algorithm 이 필요하다. 이에 본 연구에서는 그래프데이터 저장 방법을 제안하고, regular path query 수행시 발생하는 cost 및 오버 헤드를 분석 한다.

수입 냉동새우에서 검출된 WSSV의 유전학적 근연관계 조사

최소원 ( So Won Choi ),백은진 ( Eun Jin Baek ),최지영 ( Ji Yeong Choi ),태원준 ( Won Jun Tae ),김형순 ( Hyoung Soon Kim ),박우성 ( Woo Seong Park ),김민재 ( Min Jae Kim ),김광일 ( Kwang Il Kim ) 한국어병학회 2021 한국어병학회지 Vol.34 No.2

본 연구에서는 주요 새우 생산 국가에서 2017년 7월부터 2020년 11월 사이 생산되어 국내로 수입된 냉동 새우(29개 그룹)를 대상으로 흰반점바이러스(WSSV), covert mortality nodavirus (CMNV) 및 decapod iridescent virus 1 (DIV-1)의 검출여부를 조사하였다. 각 바이러스에 대한 nested PCR 결과, WSSV는 9개 그룹(9/29)에서 검출되었으며 CMNV와 DIV-1은 검출되지 않았다. Nested PCR에서 WSSV 양성으로 확인된 시료를 대상으로 WSSV genome variable loci로 알려진 VR 14/15 region에 대해 참조 서열들과 삽입/결손(insertion and deletion) 서열 비교 및 근연관계를 분석하였다. WSSV 양성 시료 중 1개 시료(20-CH-1 isolate, 2020년 10월 중국 생산)에서만 VR 14/15에 대한 PCR amplicon이 생성되었으며 염기서열 분석 결과, 20-CH-1 isolate는 2005년 인도에서 보고된 WSSV-IN-05-01 과 99.84%의 상동성을 보였다. 이는 과거 알려진 바와 같이 새우의 교역을 통한 국가 간 WSSV가 확산되었음을 뒷받침해주는 결과이다. In this study, of the imported shrimps between 2017 and 2020, we investigated white spot syndrome virus (WSSV), covert mortality nodavirus (CMNV) and decapod iridescent virus 1 (DIV-1). Of the imported shrimps (a total of 29 groups), WSSV was detected as 31% (9/29) by nested PCR assay. And CMNV and DIV-1 were not identified in this study. To investigate the genetic relatedness of WSSV identified from imported shrimp, VR 14/15 region showed WSSV genomic variable loci was compared with reference isolates. Among the nine WSSV-positive samples, VR 14/15 region was amplified in only a sample (20-CH-1 isolate, imported from China in 2020). And the 20-CH-1 isolate showed 99.8% identity with WSSV-IN-05-01 which was reported in India in 2005, suggesting that those of WSSV have been spread from India to China. Furthermore, although the pathogenicity of WSSV identified from frozen shrimp was not evaluated, the international trade of diseased frozen shrimps could be led to the potential risk of virus transmission.