발생중인 생쥐 치아 및 치주조직에서 치주인대-특이 단백질; PDLs22의 발현

박중원,박병기,김상목,김병옥,박주철,Park, Jung-Won,Park, Byung-Ki,Kim, Sang-Mok,Kim, Byung-Ock,Park, Joo-Cheol 대한치주과학회 2002 Journal of Periodontal & Implant Science Vol.32 No.1

The periodontal ligament(PDL) is a unique tissue that is crucial for tooth function. However, little is known of the molecular mechanisms controlling PDL function. PDL-specific protein;PDLs22 had been previously identified as a novel protein isolated from cultured human PDL fibroblasts using subtraction hybridization between human gingival fibroblasts and PDL fibroblasts. The aim of this study was to examine the expression pattern and tissue localization of PDLs22 protein in embryonic and various postnatal stages of developing mouse using immunohistochemical staining. Embryos (E18) and postnatal (P1, P4, P5, P15, P18) were decapitated and the heads were fixed overnight in a freshly prepared solution of 4% paraformaldehyde. Some specimens were decalcified for $2{\sim}4$ weeks in a solution containing 10% of the disodium salt of ethylenediamine-tetraacetic acid (EDTA). Next, tissues were dehydrated, embedded in paraffin and sectioned serially at $6{\mu}m$ in thickness. Polyclonal antiserum raised against PDLs22 peptides, ISNKYLVKRQSRD, were made. The localization of PDLs22 in tissues was detected by polyclonal antibody against PDLs22 by means of immunohistochemical staining. The results were as follows; 1. Expression of PDLs22 protein was not detected in the tooth germ of bud and cap stage. 2. At the late bell stage and root formation stage, strong expression of PDLs22 protein was observed in developing tooth follicle, osteoblast-like cells, and subodontoblastic cells in the tooth pulp, but not in gingival fibroblasts, ameloblasts and odontoblasts of tooth germ 3. In erupted tooth, PDLs22 protein was intensely expressed in PDL and osteoblast-like cells of alveolar bone, but not in gingival fibroblasts, mature osteocytes and adjacent salivary glands. 4. In the developing alveolar bone and mid-palatal suture, expression of PDLs22 protein was seen in undifferentiated mesenchymal cells and osteoblast-like cells of developing mid-palatal suture, but not in mature osteocytes and chondrocytes. These results suggest that PDLs22 protein may play an important role in the differentiation of undifferentiated mesenchymal cells in the bone marrow and PDL cells, which can differentiate into multiple cell types including osteoblasts, cementoblasts, and PDL fibroblasts. However, more researches should be performed to gain a better understanding of the exact function of PDLs22 protein which related to the PDL cell differentiation.

국내 분리 토끼출혈병 바이러스(RHDVa)를 감염시킨 토끼 간장에서의 경시적인 초미세구조 변화와 apoptosis

박중원,전지은,박은정,김한,이명헌,황의경,김재훈,이중복,우계형,Park, Jung-Won,Chun, Ji-Eun,Bak, Eun-Jung,Kim, Han,Lee, Myeong-Heon,Hwang, Eui-Kyung,Kim, Jae-Hoon,Lee, Chung-Bok,Woo, Gye-Hyeong 대한수의학회 2013 大韓獸醫學會誌 Vol.53 No.1

In this study, to understand the pathogenesis of new rabbit hemorrhagic disease virus (RHDVa) serotype, we carried out to administrate RHDVa to rabbits, and to examine sequential electron microscopic changes and relationship between pathogenesis and apoptosis. TUNEL-positive cells began to be observed from 24 hours after inoculation (HAI) and the number of positive cells was slightly increased with the course of time. Whereas marked increase of positive cells was seen in the liver from the rabbits died acutely. Typical viral particles with cup-like projections and a diameter of 30~40 nm were detected in homogenized liver samples and tissues at 36 and 48, and 48 HAI, respectively. Ultrastructurally, glycogen deposition was observed from the first stage of hepatocellular degeneration by RHDVa infection and then, swelling and disruption of cristae of mitochondria by viral particles, swelling of smooth endoplasmic reticulum, vacuoles and vesicles were detected. Condensation, margination and fragmentation of chromatin were observed in degenerative hepatocytes at 36 and 48 HAI, indicating apoptotic bodies. These data offer that hepatocytic apoptosis by RHDV infection could be closely related with mitochondrial impairment in the hepatocytes.

디자인·공예 융합 교육 프로그램 사례연구 -국민대학교 「팀팀Class」를 중심으로-

박중원,Park, Jung-won 한국디지털정책학회 2021 디지털융복합연구 Vol.19 No.8

융합이라는 시대의 흐름과 더불어 상상력과 창조성에 기반을 둔 디자인·공예의 본질적 속성의 효과적 결합을 위해서는 학문적, 방법적 측면에서 다양한 시도가 필요하다. 본 연구는 2020년 2학기에 실행된 국민대학교 팀팀Class의 디자인·공예 융합 교육 프로그램의 운영 사례를 분석하는 것이다. 이를 위해 기존 문헌연구를 통한 디자인·공예 융합 교육 관련 중요 항목을 5가지(대상, 방법, 운영, 공간, 소통)로 구분해 분석하였다. 이를 통해 디자인·공예 융합 교육 프로그램에 대한 경험과 문제점, 그리고 향후 디자인·공예 융합 교육 프로그램의 운영에 적용 가능한 해결방안 제시에 본 연구의 목적이 있다. 본 연구에서는 국민대학교 팀팀Class 디자인·공예 융합 교육 프로그램으로 사례연구 대상을 한정하였으나 향후 지속적인 연구를 통해 향후 다가올 코로나(COVID-19) 이후 시대의 새로운 환경에 적용 가능한 디자인·공예 융합 교육 프로그램을 위해 후속 연구가 진행되어야 할 것이다. The tendency of the current times require education to focus on convergence, and the same applies to the essence of ceramics and design base imagination and creativity. For effective integration, a wide range of experimentations is required both in terms of academic and methodic approaches. This study analyzes the [TeamTeam Class] curriculum, converging ceramics with design initiated in the second semester (autumn semester) of 2020. Through reference materials on ceramics and design convergence education, it was possible to classify the following 5 categories: Subject, Method, Management, space and communication. The aim of the study is to find resolutions to overcome existing issues and problems in search of more effective methods. Although this study is based on convergence education, [TeamTeam Class] at Kookmin University, I hope to extend it further to also consider education after COVID-19.

새로운 국내 분리 토끼출혈병바이러스(RHDVa)를 감염시킨 토끼에서의 경시적인 병리학적 변화와 조직 내 바이러스 항원 분포

박중원,전지은,양동군,박은정,김한,이명헌,황의경,이중복,우계형,Park, Jung-Won,Chun, Ji-Eun,Yang, Dong-Kun,Bak, Eun-Jung,Kim, Han,Lee, Myeong-Heon,Hwang, Eui-Kyung,Lee, Chung-Bok,Woo, Gye-Hyeong 대한수의학회 2012 大韓獸醫學會誌 Vol.52 No.2

Rabbit hemorrhagic disease is a highly acute and fatal viral disease caused by rabbit hemorrhagic disease virus (RHDV). Since first outbreak in Korea 1987, RHDV has been continually affected in the country, but the pattern of outbreak seem to be changed. In this study, to understand the pathogenesis of the new RHDVa serotype, we therefore carried out to inoculate RHDVa to rabbits, and to examine the sequential histopathologic changes and viral distribution. Macroscopically, various sized dark red or white spots or appearance were observed in the liver, lung, kidney uterus and ureter. In euhanized rabbits, significant pathologic findings such as infiltration of heterophils and mononuclear cells were observed at 24 hours after inoculation (HAI), and these were sequentially extended periportal to centrilobular area. However, in dead rabbits, severe hepatic degeneration and/or necrosis with relatively weak inflammatory responses were observed. RHDV antigens began to detect in liver, spleen, and lung from 12 HAI by PCR. Immunohistochemically, RHDV positive cells were seen in only liver from 24 HAI, and the degree of immunogen reactivity was stronger in dead rabbits than in euthanized ones. In conclusion, RHDVa caused the subacute or chronic infection accompanying low mortality and moderate to severe inflammatory reaction in rabbits, suggesting the possibility that RHD could become endemic.

심내막염 환자의 동종이식편 치환술 -1례 보고-

박중원,박국양,김웅한,Park, Jung-Won,Park, Guk-Yang,Kim, Ung-Han 대한흉부심장혈관외과학회 1997 Journal of Chest Surgery (J Chest Surg) Vol.30 No.8

Prosthetic valve endocarditis(PVE), although uncommon, is associated with significant mortality if the infection spreads into the paravavular structures with later abscess formation. However, combined antibiotic and surgical treatment is often successful. Accurate diagnosis by on echocardiography, effective myocardial protection during operation and increased surgical experience have improved the short-term and long-term outcomes for patients with PVE. A 35-year-old male had a history of replacement of aortic and mitral valve, and tricuspid annuloplasty on August 1994, was admitted due to sudden onset of aphasia, leftward deviation of both eyeballs and spiking fever and diagnosed of having PVE by echocardiography. Reoperation was done after 6weeks of antibiotic treatment. On the operative field, we could notice circumferential vegetation along aortic valve annulus, paravalvular leakage and abscess pocket. The mitral valve amlulus was healthy. The patient underwent redo aortic valve replacement using cryopreserved aortic homograft after radical debridement of infected issue. During the follow up of 7 months period the homograft was well functioning without recurrence of symptoms. 인공판막심내막염은 드물지만 합병증으로 판막주위가 감염으로 파괴되고 농양이 형성되는 경우가 있어 높 은 사망율을 가져온다. 그러나 적절한 항생제의 사용, 심장초음파 검사에 기초를 둔 조기 진단,수술시 효과적인 심근보호. 그리고 수술경험의 축적으로 인공판막심내막염에 있어서 보다좋은 장단기 결과를 얻을 수 있다. 35세 남자 환자가 94년 8월에 대동맥판막, 승모판막대치술과 함께 삼첨판륜성형술을 받은후 특별한 증상 없이 지내다가 갑자기 시작된 실어증, 양안구의 좌측편위, 고열로 입원하여 심장초음파 검사를 시행한 결과 인공판막 심내막염 소견을 보여 6주간의 항생제 치료후 수술을 시행하였다. 수술소견상 대동맥 판륜을 따라 증식물 소견과 판막 주위의 누출, 농양주머니가 형성되어 있었고 승모판막은 비교적 건강해 보였다. 수술은 감염된 조직의 전체적인 제거와 냉동저장된 대동맥 동종이식편을 이용하여 재대치 수술을 시행했다. 환자는 수술후 63일째 퇴원했으며 퇴원후 7개월 동안 외래 추적 관찰결과 특별한 이상소견 없이 지내고 있다.

집먼지진드기에 감작된 천식 환자의 말초혈액 단핵구의 항원 자극에 따른 IL - 5와 IL - 10 생성

박중원(Jung Won Park),홍천수(Chein Soo Hong),고시환(Si Hwan Ko),김철우(Cheol Woo Kim) 대한천식알레르기학회 1999 천식 및 알레르기 Vol.19 No.4

Background: IL - 5 has been recognized as a potent proeosinophilic cytokine and IL-10 has been reported as an important anti - inflammatory cytokine in allergic inflammation. But the clin - ical roles of these cytokines in allergic asthma are still unclear. Objectives : We studied the clinical implications of IL - 5 and IL - 10 secretions from stimulated peripheral blood mononuclear cells ( PBMC ) in Dermatophagoides farinae ( DF ) atopic asthmatics ( BA ). Methods : Thirty - six DF sensitized BA and 9 non - atopic BA were enrolled for this study. Twenty - seven out of 36 subjects were challenged by inhalation of DF crude allergen. The isolat - ed PBMCs were cultured for 6 days with DF antigen and the stimulatory index ( SI ) and secre - tions of IL - 5, IFN - y and IL - 10 from PBMC were measured. We analyzed these parameters with clinical parameters. Results : SI (4.2 ± 1.0 vs. 1.3 ± 0.3, p<0.05) and secretions of IL - 5 ( 19.9 vs. 1.7 g/L, p < 0.001 ) and IL-10 ( 185.5 vs. 34.3 g/L, p(0.05 ) from the atopic BA were significantly higher than those of non-atopic BA, but the secretion of IFN - r was not different between the two groups ( 56.6 vs. 47.3 ug/L ). No significant difference in secretions of IL - 5, IL - 10, IFN - r and SI of PBMC was found between responder and non - responder of DF inhalation challenge test. Among respo- nders to antigen challenge test ( n = 17 ), the production of IL - 5 correlated with the productions of IL - 10 (r = 0.773, p < 0.01 ) and methacholine PC20 ( r = 0.503, p < 0.05 ). Production of IL - 5 from the PBMC of atopic mild intermittent BA ( n = 10 ) was higher than that of atopic per'sistent BA ( n = 27 ) ( p < 0.01 ), but no difference in IL - 10, IFN - r and SI was found between the two groups. Conclusions : Allergen specific productions of IL - 5 and IL - 10 from the PBMC may be specific for atopic subjects and secretion of IL - 5 from the stimulated PBMC may contribute to the path - ogenesis of atopic BA. The severity of BA may be more influenced by other factors.

10,000 Ton Brake Bending Press 구조 건전성에 관한 평가

박중원(Jung-Won Park),이현민(Hyun-Min Lee),구태완(Tae-Wan Ku),김정(Jeong Kim),강범수(Beom-Soo Kang) 대한기계학회 2008 대한기계학회 춘추학술대회 Vol.2008 No.11

Thick pipes have been used in fields of ocean and industrial plants, and pipe arrangement of oil and water pipelines. Mostly, roll bending process has been used in manufacture of the pipes. Recently, however, studies on the pipe forming processes using brake press have been performed. Normally, the brake press has high aspect ratio, so structural integrity evaluation should be conducted. In this study, finite element analysis of structural integrity for the brake bending press was carried out for thick pipe forming process.

대한간학회지 1999년 추계학술대회 초록집 : 간에서의 아포토시스: 현재와 전망

박중원 ( Park Jung Won ) 대한간학회 1999 Clinical and Molecular Hepatology(대한간학회지) Vol.5 No.3(S)

가토 각막 표층 뮤신의 Lectin 염색에 의한 성분 분석

박중원(Jung Won Park),김응권(Eung Kweon Kim),김수진(Su Jin Kim),강신정(Shin Jeong Kang),김형래(Hyung Lae Kim),임지현(Ji Hyun Lim) 대한안과학회 1999 대한안과학회지 Vol.40 No.7

호흡기 알레르기 환자에서 광촉매플라스마 정전기 흡착식 공기정화기의 효과

박중원(Jung Won Park),고시환(Si Hwan Ko),윤영연(Yeong Yeon Yun),김철우(Cheol Woo Kim),홍천수(Chein Soo Hong),이기영(Ki Young Lee),홍영기(Young Ki Hong),이성화(Sung Hwa Lee) 대한천식알레르기학회 1999 천식 및 알레르기 Vol.19 No.1

Objective: To evaluate the clinical efficacy of a new air cleaning device (LG Electronics, Korea) equipped with electrostatic precipitator (EP) and photocatalytic plasma filter (PCP) in 24 respiratory allergic subjects. Material and method: Air cleaning conditioner and air cleaner were placed in living room and bedroom. Both EP and PCP (EP/PCP) filters were used for the first 4 weeks and for another 4 weeks, PCP without EP filter (PCP) was used. We measured symptom scores of asthma and rhinitis, peak expiratory flow rate (PEFR), serum eosinophil cataionic prote in? in ECP, Dermatophagoides farinae (DF) specific IgE and IgG in sera, concentrations of major allergens of DF in floor dust of living room, bedroom and in airborne dust. Results: Both asthma and rhinitis symptom scores were significantly improved not only with EP/PCP filters but also with PCP filter. The morning PEFR was significantly improved with EP/PCP filters, but not with PCP filter. Frequency of salbutamol inhalation by air cleaner with EP/PCP filter tended to de crease(p=0.051), with no significant difference in serum ECP concentration. DF specific IgE significantly decreased at the 8th week with no differences in specific IgG. However, the mean Der f I levels in floor dust of bedroom (1, 128 vs. 374 ng/gm dust, p<0.01) and living room (1,516 vs 812 ng/gm dust, p<0.01) decreased, Der f1 in airborne dust measured only in 8 out of 22 subjects, and they decreased significantly with the trial of EP/PCP filter (172.2 pg/4.5m vs. 62.1 pg/4.5m, p<0.01). Conclusion: These results suggest that the EP/PCP air cleaner may be an effective tool for environmental control in respiratory allergic subjects.