Applications of microwave system on surface modification reaction

문효영,서현정,김양선 한국공업화학회 2011 한국공업화학회 연구논문 초록집 Vol.2011 No.-

교육실습에서의 수학 수업이 초등예비교사의 수학에 관한 신념에 미치는 영향

문효영,권성룡 한국초등수학교육학회 2010 한국초등수학교육학회지 Vol.14 No.2

The purpose of this study was to investigate and analyze the effect of mathematics teaching experience during the teaching practicum on pre-service elementary teachers' beliefs about mathematics. The conclusions drawn from the entire research were, as follow:First, it can be said that mathematics teaching experience during the teaching practicum has a significant effect on the change of pre-service elementary teachers' beliefs about mathematics. Specifically, the teaching experience during the teaching practicum has statistically significantly negative effects(p=.05) on pre-service elementary teachers' beliefs about the teaching mathematics. Second, the factors which help pre-service elementary teachers the most in preparing for mathematics classes are collaborating teachers in charge of supervising them, the teacher's guidebook and materials acquired from the Internet. Third, pre-service elementary teachers are well aware of the importance of understanding students and emphasize concrete manipulative activities, but experience lots of failures due to difficulty of drawing students' attention. Fourth, collaborating teachers do not play a significant role in helping pre-service elementary teachers develop and change their beliefs about mathematics positively. The advise given by collaborating teachers to pre-service elementary teachers is mostly about simple techniques of managing the classroom. So, collaborating teachers do not affect significantly and positively on the change of pre-service elementary teachers' beliefs. Fifth, regardless of their belief tendency, pre-service elementary teachers teach more confidently and feel more satisfactory when they prepared for classes more thoughtfully and understanded students more deeply. 대개의 수학교실에서 교사는 자신의 수학 신념에 바탕을 두고 학생들에게 수학을 어떻게 가르칠 것인지를 결정하게 된다. 교사의 수학 신념은 과거의 다양한 수학에 대한 경험으로부터 만들어지기에 초등예비교사의 수학 신념은 교육실습을 통해 유의미한 변화가 있을 것이라 예상할 수 있다. 따라서 초등예비교사들의 수학에 관한 신념이 교육실습 전․후로 어떻게 변화되어 가는지 분석해 보고, 나아가 이들의 수학에 관한 신념 변화에 영향을 미치는 요인을 찾아보았다. 초등예비교사들을 대상으로 한 교육실습 전과 후의 설문조사 및 소수 대상자들을 교육실습 과정에서 관찰조사 한 결과, 교육실습은 예비교사들의 수학 신념 변화에 영향을 미치고 있으며, 그들이 지닌 신념에 따라 수업의 방향이 달라지고, 지도교사나 수업에서의 성공과 실패 요인 등이 신념 변화에 큰 영향을 미침을 알 수 있었다. 이 연구는 위의 결과를 바탕으로 교육실습 과정에서 초등예비교사들의 수학 신념 변화에 대한 논의 및 제언을 담고 있다.

COVID-19의 진단 기술 및 전망

문효영 한국생물공학회 2020 BT NEWS Vol.27 No.2

물건 들기와 내리기 수행 시 신발 뒤굽 조건이 요부와 둔부 신전근 활성도에 미치는 영향

김아람,문효영,이승비,박현주 한국신경재활학회 2012 한국신경근육재활학회지 Vol.2 No.1

Solid-phase Synthesis of Folate–Chlorin Conjugates for Selective Photodynamic Therapy and the Effect of Linker Variation

이선민,안영덕,문효영,고병숙,김태영,서지원,김민곤 대한화학회 2016 Bulletin of the Korean Chemical Society Vol.37 No.12

Here, we evaluated the effect of linkers conjugating a chlorin-based photosensitizer, 2-[1-hexyloxyethyl]-2-devinyl pyropheophorbide-a (HPPH), and folic acid to target cancer cells. Folate–HPPH conjugates with peptide, polyethylene glycol (PEG), peptoid, or 7-aminoheptanoic acid (AHA) linkers were synthesized on a solid-phase and evaluated for photodynamic therapy (PDT) efficacy. Compared to free HPPH, the folate-linker-HPPHs were effectively internalized into tumor cells and exhibited superior PDT effect upon irradiation. Tumor-targeting PDT efficacy was influenced by linker variation, with in vitro photocytotoxicities of peptide > PEG > AHA > peptoid, paralleling water solubility of the linkers. Thus, linker choice significantly affects targeted PDT efficacy providing useful information for linker design.

유기인계 및 카바메이트계 농약 신속 검출을 위한 역 Y자 스트립의 개발

이정은,김솔아,박현진,문효영,심원보 한국식품위생안전성학회 2021 한국식품위생안전성학회지 Vol.36 No.1

본 연구에서는 농약의 음성시료에서는 acetylcholinesterase와 acetylthiocholine을 반응시켜 +전하와 -전하를 가지는 thiocholine으로 분해되어 금나노입자를 응집시켜 역 Y자 스트립상에서 청자색의 반응선(띠)을 형성하고 양성 시료에서는 생성시키지 않는 원리를 이용한 신속 농약 검출법을 개발 하였다. 개발한 분석법은 유기인계 농약 말라옥손과 카바메이트계 농약 카보퓨란을 각각 10 ng/mL 수준까지 검출이 가능한 것으로 확인되었으며, 2종의 유기인계와 카바메이트계 농약(EPN, dichlorvos)에 대해 추가적으로 검출 한계를 확인한 결과에서도 10 ng/mL 수준까지 모두 검출 가능함을 확인 할 수 있었다. 그러나 3종의 트리아진 계열의 농약과 각 1종의 피레스로이드, 카복사마이드, 페닐아마이드 및 유기염소 계열의 농약에 대해서는 반응성이 없는 것으로 확인되어 유기인계와 카바메이트계 농약 분석에 적용이 가능한 것으로 확인되었다. 마지막으로, 임의로 오염시킨 농산물 시료를 대상으로 분석법의 회수율을 확인한 결과, 말라옥손에 대해서 96.4에서100.7%, 카보퓨란은 81에서112.7%의 회수율이 확인되어 본 연구에서 개발한 역 Y자 스트립을 농약 검출법으로 이용한다면 농산물과 농업환경 중 존재하는 유기인계 및 카바메이트계 잔류농약을 신속하게 검출할 수 있을 것으로 판단된다. The inverted Y-shaped strip detection method based on acetylcholinesterase (AChE) was developed for the rapid detection of organophosphorus and carbamate pesticides. The inactivation of AChE by organophosphorus and carbamate pesticides has been well known. The AChE catalyzes acetylthiocholine into thiocholine having (-) and (+) charges, and the (+) charge results in aggregation of gold nanoparticle (GNP). Malaoxon and carbofuran were used as standard organophosphorus and carbamate for the development of the inverted Y-shaped strip, respectively. In order to optimize the method, various angles of the Y-shaped strip, different types of nitrocellulose membrane, and concentration of AChE were tested as key parameters. The detection limit of the method was 10 ng/mL for both malaoxon and carbofuran pesticides. No cross-reaction was observed to other pesticides such as atrazine, cyanazine, simazine, bifenthrin, boscalid, metalaxyl, and chlorobenzilate. Recoveries from lettuce spiked when known concentrations of malaoxon and carbofuran were found ranging from 96.4 to 100.7% and 81 to 112.7%, respectively. This study suggests that the inverted Y-shaped strip method based on AChE may be a useful tool for the sensitive, specific, rapid detection of organophosphorus and carbamate pesticides in agricultural products.

금 나노 입자 응집 원리를 이용한 유기인계와 카바메이트계 비색-신속 농약검출법 개발

김효인,이정은,김솔아,문효영,조성래,심원보 한국식품위생안전성학회 2019 한국식품위생안전성학회지 Vol.34 No.3

A colorimetric rapid detection method based on acetylcholinesterase (AChE) was developed for the analysis of organophosphorus (OP) and carbamate (CB) pesticides. The AChE catalyzes acetylthiocholine into thiocholine having (-) and (+) charges, and the (+) charge results in gold nanoparticle (GNP) aggregation. The in-activation of AChE by OP and CB has been well known. In order to optimize the colorimetric method, optimal dilution times of commercial serum containing AChE, diameter of GNP, and concentration of acetylthiocholine were tested as a key parameter. The colorimetric detection limits of the method were 7.5 ng/mL for both dimethyl amine and carbofuran pesticides in 60% ethanol. No cross-reaction to other chemicals, such as aflatoxin B1 and ochratoxin A, which can be contaminated with pesticides in agricultural products, was observed. Recoveries from lettuce, sesame leaf, and cabbage lettuce spiked with known concentrations of dimethyl amine and carbofuran were found to be ranged from 83.85 to 133.16%. These results indicated that the colorimetric rapid method based on AChE can be a useful tool for the sensitive, specific, rapid, and accurate detection of OP and CB pesticides in fresh vegetables.

신선 고추의 수확, 선별 및 유통 단계에서의 미생물 모니터링 및미생물 오염 저감화 방법 모색

김솔아,이정은,박현진,이상대,문효영,심원보 한국식품위생안전성학회 2020 한국식품위생안전성학회지 Vol.35 No.1

This study determined the microbial contamination levels of hot pepper at harvest, sorting, and distribution stages to suggest a way of reducing the microbial contamination of hot peppers by changing work gloves used throughout these processes. According to the monitoring results, the contamination levels of total aerobic bacteria (TAB) were found in the following order: soil (5.3±0.9 log CFU/g), hot pepper (4.2±0.9 log CFU/g), gloves (4.2±0.6 log CFU/g), baskets (4.1±0.7 log CFU/ g), clippers (3.9±0.6 log CFU/ g) and water (3.2±1.1 log CFU/g) at harvest stage. The contamination level of coliforms were found in the following order: soil (2.2±0.9 log CFU/g), hot pepper (2.2±0.3 log CFU/g), gloves (2.1±0.6 log CFU/ g), clippers (2.0±0.21 log CFU/ g) and baskets (1.9±1.1 log CFU/ g) at harvest stage. TAB on hot pepper at the harvest stage was reduced from 4.2±0.9 log CFU/g to cold storage 3.8±0.2 log CFU/g and room temperature storage 2.6±0.3 log CFU/g, respectively. By the replacement of work gloves and lower distribution temperature, TAB levels of the peppers were significantly reduced compared to those without replacement and distributed at room temperature. In addition, the utilization of plasma was effective on reducing microbial contamination of hot pepper. These results demonstrated that appropriate replacement of gloves at the harvest stages, using plasma in the distribution stage, and refrigerated distribution conditions, which are simple and easy to practice in the field, are effective to reduce microbial contamination on hot peppers.