더러브렛 경주마의 유전능력평가에서 주파기록에 대한 동기군의 조성

도경탁,박경도 경상대학교 농업생명과학연구원 2019 농업생명과학연구 Vol.53 No.4

본 연구는 2012년 1월부터 2019년 1월까지 과천경마공원에서 경주한 더러브렛, 6,762두의 주파기록, 총 82,796개를 이용하여 동기군의 구성을 조사하기 위하여 수행되었다. 모든 경주거리에서 우승마의 주 파기록이 1초 감소하면 해당 경주의 평균 주파기록은 0.89초에서 0.99초 감소하였다. 주파기록에 대한 동기군의 효과는 총변이의 약 41%를 차지하였으며, 참가경주의 효과는 일괄적으로 모든 경주거리에서 가장 크게 나타났다. 동기군을 포함한 모형(Ⅰ)과 우승마 기록을 포함한 모형(Ⅱ)에서 추정된 주파기록에 대한 유전분산은 각각 0.4760과 0.4148, 유전력과 반복력은 각각 0.26, 0.47과 0.22, 0.40이었다. 모형 Ⅱ에서 추정된 유전분산과 유전력은 모형 1에 비하여 각각 13%와 15% 감소하는 것으로 나타났으며, 우승마의 주파기록을 환경효과로 모형에 포함하는 것보다는 동기군을 포함하는 것이 주파기록을 량하는데 있어서 더 유리할 것으로 판단되었다. This study was undertaken to investigate concatenation of the contemporary groups influencing finish time of Thoroughbreds, using a total of 82,796 records of 6,762 heads that raced at Gwacheon racecourse from January, 2012 to January, 2019. In all distances, when winner’s finish time decreased by one second, the average finish time of individual race in corresponding distances decreased by 0.89-0.99 seconds. The effects of contemporary groups accounted for approximately 41% of the total variation, and the effect of the individual race was the biggest in all distance. The estimates of the genetic variances for the finish time estimated from model I in which contemporary group effects were included and from model II in which winner’s time effects were included were 0.4760 and 0.4148, respectively. The estimates for the heritability and repeatability from model I were 0.26 and 0.47, respectively and those from model II were 0.22 and 0.40, respectively. The estimates for genetic variance and heritability from Model II were lower than those from the model I by 13% and 15%, respectively, and it would be more advantageous for the improvement to include contemporary group effects than to include the winner’s time effect as the environmental effects in the model.

한우에서 모색관련 유전자 변이에 관한 연구

도경탁 ( K. T. Do ),신희영 ( H. Y. Shin ),이종혁 ( J. H. Lee ),김내수 ( N. S. Kim ),박응우 ( E. W. Park ),윤두학 ( D. H. Yoon ),김관석 ( K. S. Kim ) 한국동물자원과학회 2007 한국축산학회지 Vol.49 No.6

An Approach to Identify Single Nucleotide Polymorphisms in the Period Circadian Clock 3 (PER3) Gene and Proposed Functional Associations with Exercise Training in a Thoroughbred Horse

Kyoung-Tag Do(도경탁),Byung-Wook Cho(조병욱) 한국생명과학회 2015 생명과학회지 Vol.25 No.11

주기성 시계 유전자 3(period circadian clock gene 3, PER3)는 포유류에서 생물학적 주기 타이밍 시스템의 역할을 수행 한다. 이 유전자는 규칙적인 운동 체계에 의해 근육에서 전사 개시 되는 것으로 알려져 있다. 인간과 마우스에서는 본 유전자에 대해 잘 알려져 있지만, 주기 및 연주기 동안 낮의 길이에 영향을 많이 받는 말에서 운동연관 연구는 존재하지 않는다. 운동 시 근육의 기능에 중요한 역할을 하는 PER3 유전자에 대해 대표적인 경주마인 국내산 더러브렛 품종의 운동 전과 운동 후 유전자 발현을 분석하기 위해 본 연구를 수행하였다. 그 결과, 골격근에서 PER3 유전자의 발현은 운동 전에 비해 운동 후에 유의적으로 증가하는 것으로 나타났다. 또한, 인실리코상에서 4개의 비동의성 단일 염기 변이(non-synonymous single nucleotide polymorphism, nsSNP) 분석과 이러한 nsSNP의 단백질 구조 및 기능 분석 결과, 전체 자유 에너지와 RMSD 값은 돌연변이의 원인이 될 수 있음으로 나타났다. 이 중, nsSNP–s395916798 (G72R)은 구조적 기능적 측면에서 중요한 잔기의 안정화 효과와 연관된 것을 알 수 있었다. 본 연구는 운동에 따라 더러브렛 골격근 내 PER3 발현 차이는 운동이라는 표현형에 대표될 수 있음을 확인하였다. 또한, SNP의 조합을 활용하여 운동 후 경주마의 조기 회복의 평가 지표로써 유용한 바이오마커가 될 수 있음을 시사한다. The period circadian clock gene 3 (PER3) plays a role in the mammalian circadian clocksystem. A regular exercise regime may affect the PER3 transcription in skeletal muscle. Although the effects of day length on circadian and circannual processes are well established in humans and mice, the influence of exercise on these processes in the horse has not been investigated. The present study investigated the expression of the PER3 gene following exercise in a thoroughbred breed of Korean horse. In addition, a comprehensive in silico nonsynonymous single nucleotide polymorphism (nsSNP) analysis of the horse PER3 gene and predicted effects of nsSNPs on proteins were examined. The expression of PER3 in skeletal muscle was significantly upregulated after exercise. Four nsSNPs were functionally annotated and analyzed by computational prediction. The total free energy and RMSD values of PER3 gene showed causative mutations. The results showed that nsSNP s395916798 (G72R) was associated with residues that have stabilizing effects on structure and function of PER3 gene. This study documented role of PER3 gene in phenotypic adaptation related to exercise in skeletal muscle. Further, the SNPs in PER3 could serve as useful biomarkers of early recovery after exercise in racehorses.

Draft genome sequence of Streptococcus sp. strain NM isolated from head and neck cancer patients

김영석,도경탁,박수제,Kim, Young Suk,Do, Kyoung-Tag,Park, Soo-Je The Microbiological Society of Korea 2019 미생물학회지 Vol.55 No.1

Firmicutes에 속하는 Streptococcus sp. strain NM을 두경부암 환자로부터 분리하였다. 본 연구에서는 약1.90 Mb의 크기와 39.3%의 평균 G+C 함량을 가진 NM 균주의 비완전한 유전체를 보고한다. 유전체는 1,845개의 코딩서열, 12개의 리보솜 RNA 및 58개의 전사 RNA유전자를 포함하였다. 본 유전체로 부터, 항생제내성, 용혈 및 방어시스템과 관련된 유전자들이 확인되었다. Streptococcus sp. strain NM belonging to Firmicutes was isolated from head and neck cancer patients. Here, we report the draft genome sequence of strain NM with a size of approximately 1.90 Mbp and a mean G+C content of 39.3%. The draft genome included 1,845 coding sequences, and 12 ribosomal RNA and 58 transfer RNA genes. In the draft genome, genes involved in the antimicrobial resistance, hemolysis and defense system have been identified.

C/EBPβ 와 C/EBPδ 유전자들과 더러브렛의 운동 역할에 관한 분자생물학적 분석

김정안,도경탁 강원대학교 동물생명과학연구소(구 강원대학교 동물자원공동연구소) 2018 동물자원연구 Vol.29 No.2

As a transcription factor, the CCAAT/enhancer binding protein (C/EBP) induces the expression of specific genes by interacting with their promoters. C/EBP can trigger to express other genes, because it can open chromatin structure after binding to DNA, then lead to another transcription factors. In this study, we investigated the features and post-exercise expression patterns of two genes in the C/EBP family, C/EBPβ and C/EBPδ. The basic region leucine zipper motif (bZIP) is a crucial region of C/EBP, and analysis of synonymous and non-synonymous substitution ratios revealed that bZIP regions were well conserved in mammalian species. Quantitative real-time RT-PCR data showed that C/EBPβ and C/EBPδ expression increased significantly in the muscle after exercise, but did not do so in the blood. We therefore propose that exercise-induced increased expression of C/EBPβ and C/EBPδ may stimulate gene expression in physiological pathways in muscle cells.

Complete genome sequence of Herbaspirillum sp. meg3 isolated from soil

김예은,도경탁,운노 타쯔야,박수제,Kim, Ye-Eun,Do, Kyoung-Tag,Unno, Tatsuya,Park, Soo-Je The Microbiological Society of Korea 2017 미생물학회지 Vol.53 No.4

Betaproteobacteria에 속하는 Herbaspirillum sp. meg3을 제주도 토양으로부터 분리하였다. 본 연구에서는 대략 5.47 Mb의 크기와 57.1%의 평균 G + C 함량을 가진 meg3 균주의 완전한 유전체를 보고한다. 유전체는 4,816개의 코딩 서열, 9개의 리보솜 RNA 및 51개의 전사 RNA 유전자가 존재하며, 두 개의 불완전한 프로파지 영역이 발견되었다. 또한 유전체 분석 결과는 meg3 균주가 방향족 화합물에 대한 분해능을 가지고 있음을 제시하고 있다. Herbaspirillum sp. meg3 belonging to Betaproteobacteria was isolated from soil in Jeju island. Here, we report the complete genome sequence of strain meg3 with a size of approximately 5.47 Mb and a mean G + C content of 57.1%. The genome included 4,816 coding sequences, and 9 ribosomal RNA and 51 transfer RNA genes. In the genome, two incomplete prophage regions have been identified. Also, we propose that strain meg3 has a potential capability for aromatic-compounds degradation based on the result of genome analysis.

국내 승용마의 체형상관에 따른 품종별 비교 분석

오운용,도경탁,조병욱,박경도,김성훈,이학교,신영수,조영석,Oh, Woon-Yong,Do, Kyoung-Tag,Cho, Byung-Wook,Park, Kyung-Do,Kim, Sung-Hoon,Lee, Hak-Kyo,Shin, Young-Soo,Cho, Young-Seuk 한국데이터정보과학회 2011 한국데이터정보과학회지 Vol.22 No.3

'말산업육성법' 제정에 따라 국내 승마산업의 저변확대를 위해 자질이 우수한 국내산 승용마 생산 및 개량에 대한 연구가 절실히 필요한 실정이다. 따라서 본 논문에서는 국내에서 승용마로 활용되고 있는 3품종 (웜블러드, 더러브렛, 제주산마) 32두에 대해 12항목의 체형을 측정하여, 측정자료를 바탕으로 판별분석을 실시한 결과 81.3%가 정확하게 분류되는 것을 확인 할 수 있었다. 본 연구의 결과는 향후 체구성 분석을 통한 말의 유형 (경주마, 승용마, 재활치료마, 역용마, 비육마) 및 승용마 외모 심사를 판단하는 모형 개발에 기초자료로 활용될 것이며 이는 향후 3D 영상촬영측정치를 활용한 한국형 승용마 생산 및 개량화 연구에 활용될 것으로 사료된다. There are increasing demands for the producing and breeding new domestic riding horses for the vitalizations of horse riding industry in Korea, according as 'Horse Industry Support Act' became. In this study, we were to develop the functional relation through the conformation comparison & body composition analysis. 76 heads of 5 breeds utilized for riding horses in Korea were used and their body measurements on 12 items were measured and cluster analysis was conducted to determine the correlation relation among them. The measurements were standardized that (height, croup height, pelvis length), and (hip width, width of pelvis) were highly correlated. In these results of the decision tree, we confirmed to classify the breed type determination by their body measurements (hip height, hip width, head length, croup height). This result can be used as basic data for the development of horse type determination (racing, riding, Riding for the Disabled, Working, or fattening) through the analysis of body composition, and be utilized as the basic data for the producing and breeding new domestic riding horses through the 3D Stereosocpic image system analyze.

단일염기다형성 정보를 이용한 국내 홀스타인 젖소의 유효집단 크기 추정

조광현,도경탁,박경도,Cho, Kwang-Hyun,Do, Kyoung-Tag,Park, Kyung-Do 한국데이터정보과학회 2017 한국데이터정보과학회지 Vol.28 No.3

본 연구는 홀스타인 젖소, 923두에 대한 단일염기다형성 (SNP) 42,201개를 이용하여 국내 젖소집단의 유전적 특성 및 유효집단크기를 조사하고자 실시하였다. 염색체별 인접 단일염기다형성간의 평균 연관불평형 ($r^2$)은 0.22로 추정되었으며, 14번 염색체 (0.26)에서 가장 높은 반면, 27번 염색체 (0.17)에서 가장 낮게 나타났다. SNP간의 물리적 거리가 25Kb 미만인 경우에서 $r^2$은 $0.31{\pm}0.33$으로 추정되었으며, SNP간 물리적 거리가 증가할수록 $r^2$은 현저히 감소하였다. SNP간 물리적 거리가 2.5Mb 이상에서의 $r^2$은 0.04로 25Kb 미만인 경우와 비교할 때 0.27 (87.1%) 감소하였다. 국내 홀스타인 젖소의 유효집단크기는 세대수와 비례하여 감소하는 경향을 나타내었으며, 1~5세대에서 110두로 추정되었다. In this study, we investigated the genetic characteristics and the effective population size of domestic dairy cattle using 42,201 SNPs for 923 heads of Holstein cattle. The estimate for the average linkage disequilibrium ($r^2$) among the adjacent SNPs by chromosome was 0.22, and it was highest (0.26) in chromosome 14 and lowest (0.17) in chromosome 27. When the physical distance among SNPs was less than 25Kb, the estimate for the average $r^2$ was $0.31{\pm}0.33$ and it was markedly decreased as the physical distance increased. When the physical distance among SNPs was larger than 25Mb, the estimate for the average $r^2$ was 0.04, and it decreased by 0.27 (87.1%) compared with case of physical distance of less than 25Kb. There was a trend that the effective population size in Holstein dairy cattle decreased over generations and the estimate for the effective population size in the first 5 generations (1~5th generation) was 110 heads.

제주마 보존 및 개량을 위한 연구 동향 및 고찰(총설)

임병휘,김상욱,강다원,박경도,도경탁 강원대학교 동물자원공동연구소 2019 동물자원연구 Vol.30 No.1

Jeju pony, a natural monument no. 347 living in Jeju Island, Korea, has been conserved as a genetic resource and a racing pony. A total of 125 studies written about this breed have been published in various fields, primarily animal science (106), veterinary medicine (13), and others (6), from 1961 to 2018. Specifically, the majority of studies have been published in animal breeding and genetics (84), which are subcategories in the field of animal science. However, most of the research in breeding and genetics for Jeju pony were performed in the early 2000s, and studies using recently developed techniques are inferior to other breeds (thoroughbred etc.). Our current understanding of both the conservation and utilization of the breed is still incomplete. Thus, it is important to use recently developed technologies to improve management and breeding strategies for this pony breed. In this study, we review studies on Jeju ponies in the field of animal breeding and genetics and discuss problems that need to be addressed. Reviewed studies were divided into 6 categories: systematic classification, coat color, body measurement and growth, racing performance, behavior, and effective population size. Finally, we suggest ideas for future research projects that could potentially contribute to the Jeju pony industry. -

노화촉진마우스의 텔로미어 함량 분석

이미랑(Mi-Rang Lee),도경탁(Kyoung-Tag Do),한정주(Jyung-Ju Han),문소현(So-Hyun Moon),강한석(Han-Seok Kang),김선구(Seon-Ku Kim),신택순(Teak-Soon Shin),이홍구(Hong-Goo Lee),황대연(Dae-Yon Hwang),김용균(Yong-Gyun Kim),손시환(Sea-Hwan Soh 한국생명과학회 2009 생명과학회지 Vol.19 No.10

Telomeres, comprised of tandem repeats of TTAGGG sequences, are special nucleoprotein structures that protect and stabilize chromosome ends. These structures form the crux of the telomere concept of aging, senescence and genomic instability. The classic terminal restriction fragment (TRF) analysis to quantify the amount of telomeric DNA is disadvantageous in species containing ultra long telomeres like in mice (100Kb). In this study, we used a more sensitive quantitative fluorescence in situ hybridization (Q FISH) technique to quantify telomeric DNA, and used it as a biological aging marker in mice. 12 litters each of Senescence-Resistant (SAMR1) and ?Prone (SAMP1) known as senescence accelerated mouse strains were purchased from Central Lab, Animal Inc. We quantified the amount of telomeric DNA using telomere specific DNA probes on the two strains of male mice at 8 weeks, 18 weeks and 26 weeks of age. The amount of telomeric DNA correlated with aging and age associated changes in body and organ weight between SAMR1 and SAMP1 strains of mice. These data suggest the usefulness of the amount of telomeric DNA as a biological aging marker in human aging studies.