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이병오 강원대학교 동물생명과학연구소(구 강원대학교 동물자원공동연구소) 2005 동물자원연구 Vol.16 No.-
The Korean livestock industry has grown rapidly. Also the relative importance of livestock production in the total agricultural products has also increased very much. However, there are lots of problems to solve from now because of changing circumstances around Korea, such as increasing imports of low price livestock products, decreasing growth rate of domestic food consumption, and food safety problems. New directions for the animal resource industry are summarized as follows : Firstly, the business field of livestock industry must be enlarged to the animal resource area. Secondly, food safety and sustainable system are very important sectors to develop the animal resource industry in the future. Thirdly, new cooperative systems in the production field, such as integration, support system for calf raising and manure recycling are necessary. Fourthly, promotion and marketing strategies of brand products must be improved.
동물복지의 동향과 인증제도의 정착을 위한 방안 모색(총설)
안규철,송영한,박근규 강원대학교 동물생명과학연구소(구 강원대학교 동물자원공동연구소) 2014 동물자원연구 Vol.25 No.2
Farm animal welfare means how a farm animal is living with the proper conditions. In modern society, farm animal welfare is essential for sustainable development and humane production of livestock. The initial concept of animal welfare was started from England based on five freedoms, and the animal welfare guideline of OIE (Office International des Epizooties) had become an international standard for animal welfare. OIE’s guideline dealing with transportation, disease prevention and appropriate veterinary treatment, shelter, management and nutrition, humane handling and humane slaughter of farm animal. For the settlement of farm animal welfare, it is necessary to introduce the animal welfare certification standards considering domestic situation, based on the guidelines of OIE standards. The purpose of this review was to; firstly, evaluate global status of farm animal welfare and secondly, suggest several plans for settlement of farm animal welfare in South Korea.
HNF4α 유전자 내 SNP가 한국 재래계의 성장에 미치는 효과 분석
최소영,양송이,홍민욱,손시환,정동기,홍영호,이성진 강원대학교 동물생명과학연구소(구 강원대학교 동물자원공동연구소) 2016 동물자원연구 Vol.27 No.2
The HNF4α (hepatocyte nuclear factor 4, alpha) is a hepatic transcription factor related to the lipid metabolism and regulation of insulin secretion in humans. The current study about commercial broiler reported that the A543G single nucleotide polymorphism (SNP) within HNF4α gene has an effect on fat deposition and wing yield in the chicken. This study was performed to investigate the association between the SNP within HNF4α gene and growth trait and to verify the applicability as a molecular marker for the improving the performance in Korean native chickens (KNCs). A total of 764 KNCs was collected from the livestock farm of Gyeongnam National University of Science and Technology in Korea and genotyped by PCR-RFLP. The body weight measured at birth, 2, 4, 6, 8, 10, 12, 14, 15, 16, 18, 20, 24, 28, 32, 36, and 40 weeks of age for the association analysis between chicken growth and the SNP genotype. Statistical analysis of the SNP with phenotypic traits was performed using SAS program. The KNC strain was classified by the genotypes of the A543G SNP. The frequencies of three genotypes were 0.47 (AA), 0.43 (AG) and 0.10 (GG), respectively. The SNP of HNF4α has highly significant association (p<0.001) with all-round growth in KNCs. These results suggest that the A543G SNP within HNF4α gene could be a genetic marker for the breeding in Korean native chickens.
소의 정원세포에서 Matrix Metalloproteinase-9(MMP-9) 발현 양상 구명
곽태일,이원영,정하연,이승윤,송혁 강원대학교 동물생명과학연구소(구 강원대학교 동물자원공동연구소) 2016 동물자원연구 Vol.27 No.2
Identification of specific marker proteins in cells is useful for isolating cells and determining their cellular characteristics and functions. Based on our previous study showing that matrix metalloproteinase 9 (MMP-9) can be used as a marker for porcine spermatogonia, the expression pattern of MMP-9 was determined in both pre- (5-month old) and post-pubertal (11–month old) bovine testes. Histological analysis revealed that spermatogonia were located near the basement membrane in both testes, while spermatozoa were not detected in the 5-month old pre-pubertal bovine testes and epididymides. Mature spermatozoa were observed in the 11-month bovine testes and epididymides, and MMP-9 expression in 11-month old bovine testes was lower than 5-month old testes, according to reverse transcription-PCR and real-time-PCR data. To determine the specific expression sites of MMP-9 in the bovine testes, immunohistochemistry was performed. Expression of MMP-9 was observed in cells near the basement membrane of seminiferous tubules in both 5- and 11-month old testes. Furthermore, MMP-9 positive cells expressed protein gene product 9.5 (PGP9.5) and deleted in azoospermia (DAZL) that are already known as bovine spermatogonial stem cells markers. In the present study, MMP-9 expression was identified in both pre- and post-pubertal bovine spermatogonia expressing PGP9.5 and DAZL, and located near the basement membrane of seminiferous tubules. Thus, MMP-9 can be used as a marker for bovine spermatogonia, and may provide useful platforms for understanding the interaction between germ cells and extracellular matrix during spermatogenesis in the seminiferous tubules.