Development of Molecular Markers for Bentazone-Resistant Wheat (Triticum aestivum L.) Induced by Gamma-ray Irradiation

김재윤,서용원,장철성,D. S. Kim,김대연,J. B. Kim,K-R Kim 한국물리학회 2007 THE JOURNAL OF THE KOREAN PHYSICAL SOCIETY Vol.50 No.I

Wheat mutants generated from a cultivar ``Geumgangmil"by using a $^{60}$Co source (gamma-ray) were developed for bentazone (3-isopropyl-2,1,3-benzo-thiadiazin-4-one-2,2-dioxide) resistance. Resistant M$_2$ plants were selected from seedlings and adult plants consecutively. Bentazone-resistant wheat plants were able to survive at up to 10 times (1,600 ppm) the commercially recommended concentration. The four plants with the highest bentazone resistance were selected. An AFLP (amplified fragment length polymorphism) analysis was applied to identify molecular markers for bentazone-resistant wheat mutants. Twelve polymorphic products, which were unique to bentazone-resistant plants, were identified, cloned, and sequenced. Among the twelve polymorphic bands, eight Sequence-tagged Sites (STS) primer sets were designed. Only one STS primer set (HRMW-08) was converted a ``Bentazone-resistant wheat-specific" STS marker. The wheat mutants and markers that we obtained could be employed in a cross-breeding program incorporated with marker assisted selection (MAS) in the early stages of population segregation.

직접 산화법에 의한 경사기능 재료의 제조에 관한 연구

김재윤,김경식,김석윤 ( J . Y . Kim,K . S . Kim,S . Y . Kim ) 한국열처리공학회 1996 熱處理工學會誌 Vol.9 No.4

The direct reaction method has been used for the fabrication of Al-Mg/Al₂O₃functionally gradient materials. It was found that the reaction layer of the Al-Mg/Al₂O₃powder compact at 900℃ under air atmosphere led to the formation of reaction layers with varying ceramic phase contents. As the results of experiments by using the TGA system, the characteristics and growth behavior of the reaction layers were affected by the reaction temperature, the gas flow rate, the Mg contents and the Al₂O₃contents.

국내 밀 43개 품종에 대한 아그로박테리움 형질전환 효율성 검정

김재윤,이건희,이하늘,현도윤,Jae Yoon Kim,Geon Hee Lee,Ha Neul Lee,Do Yoon Hyun 국립한국농수산대학교 교육개발센터 2024 현장농업연구지 = Journal of practical agricultural resear Vol.25 No.4

본 연구는 국내 밀 43 품종에 대한 A. tumefaciens 형질 전환 효율을 검정하기 위해 GUS staining 분석을 수행한 것으로서 대부분의 밀 형질전환 연구가 특정 품종에 국한되어 있기 때문에 국내 장려 밀 품종에 대한 형질전환 효율에 영향을 미치는 조건에 대한 검정이 필요하다. 국내 밀 품종 중 32개에서 1개 이상의 조직에 염색된 신호가 관찰되었으며 4개 품종에서는 염색된 신호가 관찰되지 않았고 6개 품종에서 과도한 A. tumefaciens 성장이 관찰되었고, 7개 품종은 미성숙배의 크기 등의 이유로 GUS staining 분석이 불가능하여 추가 분석에서 제외하였다. 국내 밀 품종별 A. tumefaciens 감염 효율 비교 결과, 백립계 8개 품종 및 적립계 28개 품종에서는 평균적으로 유의한 차이가 없었다. 특히 조농밀, 조품밀, 새올밀, 조중밀, 새금강밀 등 적립계 품종에서 90% 이상의 높은 감염 효율이 관찰되었고, 전체 품종 중 22개는 50% 이상의 효율을 나타내었다. 본 연구를 통하여 조농밀 및 새올밀은 미성숙배에서 전체 조직에서 강한 GUS 발현으로 형질전환에 적합한 품종으로 나타났으며 금강 품종은 백립계에서 상대적으로 높은 발현을 보여 A. tumefaciens을 이용한 형질전환에 적합한 것으로 확인되었다. 추가적인 연구를 통해 품종의 조직배양 효율을 검정하고, 안정적인 GUS 발현 효율을 조사하는 것이 필요하며, 이를 통해 밀 형질전환에 이용 가능한 품종을 더욱 정밀하게 선발할 수 있을 것으로 판단된다. 해당 연구 결과는 국내 밀품종의 형질전환 효율을 높이기 위한 기초 자료로 활용 가능할 것이다. Agrobacterium-mediated transformation (AMT) is a method that allows for the stable integration of DNA fragments into the plant genome. Transgenic plants generated through AMT typically exhibit a lower copy number of the transgene compared to those induced by particle bombardment. Furthermore, AMT offers a straightforward and efficient approach for generating transgenic plants. While the transformation efficiency of wheat is comparatively lower than that of other monocot plants such as Rice (Oryza sativa L.) and Maize (Zea mays L.), the cultivars 'Bobwhites' and 'Fielder' are commonly employed for wheat transformation. To date, there have been no reported instances of successful development of transgenic plants using Korean wheat varieties through AMT. This study aims to assess the transformation efficiency of 43 Korean wheat cultivars using the GUS assay, with the goal of identifying suitable Korean wheat cultivars for AMT. The pCAMBIA1301 vector, carrying the β-glucuronidase (GUS) gene, was incorporated into Agrobacterium strain EH105. Following the inoculation of Agrobacterium into immature embryos, GUS assays were conducted 'Saeol', 'Jopum', and 'Jonong' showed 100% (the number of embryos showing GUS spots/the number of embryos used for AMT) among 43 cultivars. In addition, cultivars with more than 70% were 'Saekeumgang', 'Jojung', 'Tapdong', 'Anbaek', 'Dabun', 'Sugang', 'Keumgang', 'Jeokjung', 'Seodun', 'Joeun', 'Dajung', and 'Baekjung'. It seems that the 15 cultivars above showed the possibility of using AMT. On the other hand, 'Yeonbaek', 'Goso', 'Baekgang', and 'Johan' showed less than 20% and GUS spots were not observed in 'Gru', 'Gobun', 'Milseong', and 'Shinmichal-1'. This study explores transient GUS expression in Korean wheat cultivars seven days after AMT. The observed initial high efficiency of transient transformation suggests the potential for subsequent stable transformation efficiency. Korean wheat cultivars demonstrating elevated transient transformation efficiency could serve as promising candidates for the development of stable transgenic wheat.

선박용 주기관 냉각열을 이용한 조수기 개발

김재윤,송치성,Kim, Jae-Yun,Song, Chi-Seong 재료연구소 2001 機械와 材料 Vol.13 No.3

객원기자코너-디지털 시대 왜 독서해야 하는가

김재윤,Kim, Jae-Yun 대한인쇄문화협회 2003 프린팅코리아 Vol.18 No.-

원적외선이 흰쥐의 생존율에 미치는 영향

김재윤,박승규,김진상,박래준,Kim, Jae-Yoon,Park, Seung-Kyu,Kim, Jin-Sang,Park, Rae-Joon 대한물리치료학회 2001 대한물리치료학회지 Vol.13 No.3

IR classified by wavelength three parts NIR, MIR. FIR. There is FIR which is radiated from healthy human body the wave length is 8-14m. The Sun's ray is composed of Infared(49%), Visible light(40%) and Ultra violet(11%), however the ray getting to the earth is FIR(60%), IR(20%), and UV(20%). Human beings has utilized FIR already from time immemorial. Hershel found out Infrared for the first time, in the Industrial Revolution the Infrared and FIR had been begun to use making products. FIR with low temperature can deeply penetrate on the human body composed things without troublesome, since FIR has effectively operated on the human body at low temperature (35-40 $^{\circ}$C). In this study, we experimented in the specific temperature FlR radiation intensity. water consumption rate, feed consumption rate. survival rate and mean of weight balance with FlR radiation instrument. According to the results, the FlR radiation to the mice assisted to increase the survival rate.

기초형식에 따른 홍예교의 역학적 특성분석

김재윤,차근창,한철희,박영신,김호수 한국구조물진단유지관리학회 2011 한국구조물진단학회 학술발표회논문집 Vol.2011 No.9

The stone masonry arch bridges have the behavior characteristics of arch structure. For this reason, the reasonable analysis method is reguired for the structural behavior of stone masonry arch bridges. Therefore, this study performs the mechanical characteristics analysis according to the foundation types of stone masonry arch bridges through the finite element modelling.

객원기자코너-출판.인쇄.잡지정책의 비전과 과제

김재윤,Kim, Jae-Yun 대한인쇄문화협회 2004 프린팅코리아 Vol.20 No.-

고강도 ADI 의 자동차용 캠사프트 개발

김재윤,강동명,안상욱,김광배,강인찬 ( Jae Yun Kim,Dong Myoung Kang,Sang Uk Ahn,Kwang Bae Kim,In Chan Kang ) 한국주조공학회 1991 한국주조공학회지 Vol.11 No.3

N/A The present study was conducted to estimate the fatigue crack growth rate of the Mo-Ni alloyed ductile cast iron for the influence of austempering temperature and time. The fatigue crack growth rate was affected by retained austenite. Retained austenite volume were higher with the ductile cast irons which were austempered at 360℃ than austempered at 260℃, and the fatigue crack growth rate of the former was lower than that of the latter. Also, the fatigue growth rate of the former was the lowest at austempering for 1hour and that of the latter was the lowest at austempering for 3hours.

한반도 동북지역 뇌문토기 변천과정

김재윤,Kim, Jae-youn 국립문화재연구원 2007 헤리티지:역사와 과학 Vol.40 No.-

필자는 동북지역 신석기시대에서 청동기시대로의 이행과정을 구체적으로 살펴보기 위해서 뇌문토기를 고찰하였다. 뇌문토기는 현재까지 서북지역 신석기 후기 편년의 근간이 되는 것으로, 동북지역 뇌문토기와의 비교연구를 통해 동-서 지역의 병행관계 설정이 가능한 자료이다. 따라서 서북과 동북의 자료를 모두 검토대상으로 하였다. 동북지역의 뇌문토기 계보와 변천과정을 구체적으로 알아보기 위해서 뇌문토기의 속성을 추출하고 편년작업을 실시하였다. 각 지역의 뇌문토기를 살펴본 결과, 동북지역 뇌문토기는 압록강을 중심으로 한 서북지역의 영향을 강하게 받은 것으로 생각된다. 그 중 서북지역은 3기로 나누어지는데, 속성분석에 따르면 서북지역 2기에 해당하는 뇌문토기가 동북지역에 영향을 준 것으로 판단된다. 동북지역 뇌문토기를 두만강 유역에서 초현한다. 전체적으로 4기로 나누어지는데 2기에 계열 분화되며 3기가 되면 남연해주 내륙으로 확산된다. 남부 연해주에는 뇌문토기 4기, 신석기 말기까지 존재하는 것으로 보인다. This paper is aimed to study the late Neolithic Age in North Korea in order to closely examine a transition process into the Bronze Age in the Korean peninsula. Thus, the pottery with lightning design was selected as target data. Since the pottery with lightning design is fundamental data that North Korea's archeologists have used for chronological recordings of the late Neolithic Age in the northwest region, the parallel relationship between the eastern and the western region was established with comparison of pottery with lightning design in the northeastern region. The examination focuses on data that cover the target region of the pottery with lightning design of the adjacent Southern Primorskii region including the counterpart of the northwestern and the northeastern region. As a result, some attribute analyses revealed that the pottery with lightning design was affected by the counterpart of the northwestern region near the Yalu River. Prior to genealogical and chronological recordings of the pottery with lightning design in the northeastern region, the pottery with lightning design in the northwestern region was recorded chronologically in order to examine its specific development process. Consequently, in the second period of the Neolithic Age, the pottery with lightning design in the northwestern region was assumed to have an impact on the counterpart in the northeastern region. The classification of the pottery with lightning design in the northeastern region shall be based on 4-period development. According to this classification, the pottery, which was found in the Tumen river basin, was thought to belong to the first period. The pottery went through genealogy differentiation in the second period and when entering into the third period, the pottery spread to inland of south Primorskii. The pottery was assumed to exist in the southern Primorskii region until the tip end (the fourth period) of the Neolithic Age. It is assumed that considering the fact that climate change led to the agriculture movement, Zaisanovka culture, i.e. the late Ne Neolithic Age, moved to the southern Primorskii region along the Tumen River basin.