[유해물질분과] 환경호르몬 물질(스티렌 트라이머)를 분해하는 미생물 분리 및 특성파악

고명진,류인재,강창민,정선용 한국폐기물자원순환학회 2003 한국폐기물자원순환학회 학술대회 Vol.2003 No.1

한국인 소아 및 청소년에서 IGF-I 유전자형의 분석과 cytosine-adenine(CA) repeat 유전자 다형성이 혈중 IGF-I 농도에 미치는 영향

고명진,황태규,이정녀,정우영 대한소아청소년과학회 2006 Clinical and Experimental Pediatrics (CEP) Vol.49 No.12

Purpose : The aim of the present study was to investigate the role of polymorphic cytosine adenine (CA) repeat of the IGF-I gene in the age-related alterations of serum IGF-I levels in healthy children. Methods : Two hundred and forty three normal healthy children (136 boys; 107 girls) aged between 7 and 15 years were enrolled in the present study. The primers were designed to cover the promoter regions containing the polymorphic CA repeat. Data were analyzed using GeneMapper software, version 3.7. All analyses were performed using MEDCALC software packages. Results: Deletion of 2 bp (G, A) following 3' of CA repeat were observed in all Korean children. The CA repeat sequences ranged from 17 to 23, and 19 CA repeat were the most common with an alleles frequency of 39.3 percent. Considering genotypes, 63.8 percent of subjects were homozygote or heterozygote for 19 CA repeat (192 bp allele), suggesting that this is wild type allele from which all other alleles originated in Korean children. Homozygote for 19 CA repeat were 14.7 percent, heterozygote for 19 CA repeat was 49.1 percent and 19 CA noncarriers totalled 36.2 percent. In 19 CA repeat noncarriers, the mean height, weight and serum IGF-I level were lower compared with those of 19 CA homozygous carriers, but statistically not significant. Correlations between serum IGF-I level and age according to the IGF-I genotypes revealed statistically significant relationships in the all groups, in the 19 CA repeat carrier group and, even in the noncarrier group. Conclusions : There were no significant differences of the mean height, weight and serum IGF-I levels among three different genotype groups. Also, there were no significantly different correlations between 19 CA repeat polymorphisms and serum IGF-I levels, according to genotype. Our results suggest that the IGF-I 19 CA repeat gene polymorphism is not associated with circulating IGF-I levels in healthy children. 목 적 : IGF-I 프로모터 CA repeat 유전자 다형성이 혈청 IGF-I농도에 미치는 영향에 대해서는 일치되지 않은 결과들이 보고되어 있다. 저자들은 한국인 소아 및 청소년을 대상으로 IGF-I 유전자형의 분석을 실시하였고, CA repeat 유전자 다형성이 혈청 IGF-I 농도에 미치는 영향을 조사하였다. 방 법 : 신장 계측에 의해 1998년에 제작된 한국소아 표준 신장표에 의거하여 나이에 따른 평균 신장 2표준편차 안에 속하는 소아 및 청소년 243명을 대상으로 하였다. 유전자형의 분석은 유전자 염기서열분석을 실시하였다. CA repeat 회수에 따른 대립유전자의 분포를 조사하였고, 이를 바탕으로 유전자형을 분류하였다. 결 과 : 한국인 소아 및 청소년에서는 IGF-I 프로모터 CA repeat 3' end 부위에 2 bp 크기의 결손이 있었다. CA repeat의 분포는 17부터 23까지였으며, 19 repeat가 39.3%의 빈도로 가장 높았다. 유전자형을 살펴보면 한국인 소아의 63.8%가 19 CA repeat를 하나 이상 가지고 있어서, 이 유전자형이 야생형으로 생각된다. 유전자형은 36명(14.8%)은 19 CA repeat(192 bp allele) homozygous, 119명(49.0%)은 heterozygous, 88명은(36.2%) 19 CA repeat noncarrier 였다. 유전자형에 따른 키, 체중, BMI는 세군 모두에서 유의한 차이가 없었다. 유전자형에 따른 혈청 IGF-I 농도도 19 CA noncarrier군에서 526.70±177.67 ng/mL로, 19 CA homozygous군 570.06±207.91 ng/ mL에 비해 낮았으나 세군 사이에 유의한 차이가 없었다. 유전자형과 나이에 따른 혈청 IGF-I 농도와의 상관관계를 분석하였을때 19 CA homozygote 군(r=0.7181; P<0.0001), heterozygote 군(r=0.5506; P<0.0001) 그리고 19 CA noncarrier군 모두에서 유의한 양의 상관관계를 보였다(r=0.5155; P<0.0001). 결 론 : 한국인 소아 및 청소년에서 19 CA repeat 3' end 부위에 2 bp 크기의 G, A 뉴클레오타이드의 결손이 관찰되었다. IGF-I 유전자의 CA repeat 분포는 17부터 23까지였으며, 19 repeat의 빈도가 39.3%로 가장 높았다. 키, 체중, BMI 그리고 혈청 IGF-I 농도는 유전자형에 따라 유의한 차이가 없었다. 그리고 IGF-I 유전자형과 나이에 따른 혈청 IGF-I 농도 사이에는 유전자형에 관계없이 모든 군에서 유의한 양의 상관관계를 나타내었다. 그러므로 건강한 소아 및 청소년에서는 IGF-I 유전자 다형성이 혈청 IGF-I 농도에 영향을 미치지 않는다.

Cardiac arrest after sugammadex administration in a patient with variant angina -a case report-

고명진,김용한,강은수,이병철,이수정,정재욱 대한마취통증의학회 2016 Korean Journal of Anesthesiology Vol.69 No.5

A 76-year-old man with no notable medical history was scheduled for a robot-assisted radical prostatectomy. After the operation, he was given sugammadex. Two minutes later, ventricular premature contraction bigeminy began, followed by cardiac arrest. Cardiac arrest occurred three times and cardiopulmonary resuscitation was done. The patient recovered after the third cardiopulmonary resuscitation and was transferred to the intensive care unit. Coronary angiography was done on postoperative day 1. The patient was diagnosed with variant angina and discharged uneventfully on postoperative day 8.

Characteristics of Pt thin films on WC substrate fabricated by ion beam-assisted DC magnetron sputtering for a lens glass mold

고명진,박순섭 한양대학교 세라믹연구소 2016 Journal of Ceramic Processing Research Vol.17 No.1

Pt thin films with a Cr or Ti interlayer were deposited onto a tungsten carbide (WC) substrate by ion beam-assisted directcurrent (DC) magnetron sputtering. The percentage composition of Cr and Ti underneath the Pt films varied and the completethin film characteristics were used to categorize specimens. Microstructure and surface analysis of the specimens wereconducted by scanning electron microscopy (SEM), X-ray diffractometry and atomic force microscopy. Mechanical propertiessuch as hardness and adhesion strength of Pt thin films were also examined. Interlayers of pure Ti or Cr were formed withthicknesses of 40 nm and 50 nm, respectively. The growth rates of both Cr and Ti thin films were similar when exposed to thesame deposition conditions. Analysis of the specimen SEM images revealed that all Pt thin films investigated, irrespective oftarget composition, had anisotropic grains with a dense columnar structure. Hardness and adhesion strength values of the Cr/Pt thin films were higher than those of the Ti/Pt thin films.

건물 외벽체 현장 열저항과 열관류율 평가를 위한 평균법의 수렴성 지표 분석

고명진,문지훈,김용식 한국생활환경학회 2019 한국생활환경학회지 Vol.26 No.4

This study analyzed the convergence of the in-situ thermal resistance (R-value) and thermal transmittance (U-value) obtained by the average method using criteria of ISO 9869-1 and ASTM C1155-95. Onsite measurement was conducted for an northwest-facing opaque exterior wall of a 30-year-old apartment house over a period of three weeks during winter. The in-situ R-value and U-value obtained at the end of the test are 51.1% smaller and 113.0% larger than the theoretical values calculated according to the ISO 6946. These results show that identifying the in-situ thermal performance of exterior walls is important for building energy efficiency. When using criteria of ASTM C1155- 95, the minimum measurement duration was 3 days, but the results of this period showed a deviation of approximately 10% from the values at the end of the test. On the other hand, the convergence conditions of ISO 9869-1 were satisfied continually from 17 days after the start of measurement. Therefore, the findings show that the convergence criteria of ASTM C1155-95 can reduce the test duration but it is possible to derive the results with lower confidence level, whereas the convergence criteria of ISO 9869-1 increases the test duration but can produce a more reliable results.

상전이를 이용한 기공성 urushi membrane

고명진,노서현,황승상,원종옥 한국공업화학회 2019 한국공업화학회 연구논문 초록집 Vol.2019 No.1

높은 내구성과 높은 내열성을 갖는 친환경 소재인 urushiol이 Fe³⁺ 이온 존재 하에 urushi network 고분자를 형성하는 특성을 이용해 아세톤 용매가 존재하는 상태에서 고분자화하여 urushi organogel을 최초로 제조하였다. 본 연구에서는 urushi organogel을 제조하고, 이를 물속에서 상전이(phase inversion)를 통해 asymmetric한 membrane을 제조하였다. urushiol과 Fe³⁺ 이온간의 고분자화 mechanism을 분석하기 위하여 IR과 Raman을 통해 urushiol의 catechol기에서 aromatic ether, Fe-urushiol 배위결합을 갖는 것을 확인하였다. 얻어진 urushi membrane의 형태 및 특성을 각각 SEM, porosity 분석을 통해 고찰하였다.

천연 옻을 이용한 다중 결합된 유기젤 합성과 특성 연구

고명진,정지윤,황승상,원종옥 한국공업화학회 2019 한국공업화학회 연구논문 초록집 Vol.2019 No.0

Urushiol은 카테콜기의 산화반응에 이은 고분자화 반응을 통해 내구성, 내열성이 높은 가교구조를 한 urushi network 고분자를 형성한다. 본 연구에서는 urushiol의 다양한 기능기를 이용하여 물리화학적 결합을 유도, urushi 유기젤을 합성하였다. Urushiol 농도, 반응 조건 및 반응 시간에 따른 젤 형성 조건을 확립하고, Rheometer를 통해 modulus를 측정하였다. 젤 형성시 일어나는 화학 반응은 Raman spectrometer를 측정하여 확인하였고, SEM을 통해 유기젤의 형태를 관찰하여, 유기젤 제조 조건과 특성과의 상관관계를 고찰하였다

실천 문제중심의 녹색식생활교육 프로그램이 초등학생의 식생활태도에 미치는 효과

고명진,김영희 한국실과교육학회 2012 한국실과교육학회지 Vol.25 No.2

The purpose of this study was to evaluate and verify the effects of Green Food Education Program based on the practical problem solutions to the dietary attitudes of primary school students. The experimental research design was selected for comparing the two groups' dietary attitudes. The effects of the program was analyzed with SPSSWIN 18.0 statistical program under 5% of significant level. The results of the research were as follows: First, the application of Green Food Education Program based on the practical problem solutions showed the significant differences on the each of the three areas and the whole dietary attitudes between the experimental group and the comparative group. Second, after verifying the scores of dietary attitudes on the experimental group and comparing group, there were significance differences on the three areas : environment, health, and caring. Therefore, the Green Food Education Program based on the practical problem solutions proved the positive effects on the dietary attitudes for the elementary school students. 이 연구는 실천 문제중심의 녹색식생활교육 프로그램이 초등학생의 식생활태도에 미치는 효과를 검증하는 데 그 목적이 있었다. 이를 위해 부천시 원미구 소재의 D초등학교 2학년 2개 학급을실험집단과 비교집단으로 선정하였다. 실험집단에는 학년 수준에 맞게 수정한 실천적 문제중심의수업방법과 녹색식생활의 3대 핵심가치인 환경, 건강, 배려의 내용으로 선정된 8개의 실천 문제를중심으로 하는 녹색식생활교육 프로그램을 구안하여 약 9주간에 걸쳐 적용하였으며, 비교집단에는 학년교육과정에 의한 창의적 체험활동 계획에 의해 일반수업을 실시하였다. 실험설계는 사전-사후 통제집단 설계를 사용하였고, 자료를 SPSSWIN 18.0 통계 프로그램을 이용하여 t-test를 실시하였다. 이 연구를 통하여 밝혀진 결과는 다음과 같다. 첫째, 실천 문제중심의 녹색식생활교육 프로그램을 적용한 실험집단과 적용하지 않은 비교집단의 식생활 태도 점수를 검증한 결과 식생활태도 점수에 유의미한 차이가 있었다. 둘째, 실천 문제중심의 녹색식생활교육 프로그램을 적용한 실험집단의 전후 식생활태도 점수를검증한 결과, 식생활태도 전체 및 환경, 건강, 배려의 녹색식생활태도 영역 모두에서 유의미한 차이가 있었다. 이 연구의 결과를 종합해 보면 실천 문제중심의 녹색식생활교육 프로그램은 녹색식생활을 실천하기 위해 계획하고 노력하려는 의지의 발달로 인해 환경, 건강, 감사와 배려의 식생활태도 영역에 향상을 가져와 식생활태도 전체에 긍정적인 효과를 나타냈다.

An Anatomical Clue for Minimizing Iliac Vein Injury During the Anterolateral Approach at L5–S1 Level: A Cadaveric Study

고명진,박승원,위성현 대한척추신경외과학회 2021 Neurospine Vol.18 No.4

Objective: The injury to the common iliac vein (CIV) seems to be the most important concern during the anterior approach to the spine at L5–S1 level. We investigated the anatomy of the L5–S1 vertebral structures related to the CIV through a cadaveric study to find an anatomical clue for safe dissection of CIV. Methods: Ten cadavers were prepared for this study. After removing the peritoneum and the presacral fascia, the section from the lower part of the L5 to the upper part of the S1 vertebral body was removed with the CIV attached. After decalcification, 2 sections in the vertical and horizontal directions were made for histological study. Results: An adipose tissue layer was present between the intervertebral disc and CIV. The adipose tissue layer in 6 cadavers was thin, and in 3 of these cadavers, the CIV was attached to the vertebral body and the disc. In the other 4 cadavers, the CIV was clearly separated from the vertebral body and the disc by the intervening adipose tissue layer (IATL). Under the microscope, a thin layer surrounding the anterior longitudinal ligament, periosteum, and disc was observed, and we named this structure the ‘perivertebral membrane’. The perivertebral membrane was attached to the CIV when there was no IATL, but a potential space was detected under the membrane. Conclusion: There was a thin membrane, perivertebral membrane, between the CIV and L5–S1 disc. In cases with CIV adhesion to the disc due to the absence of IATL, the CIV may be mobilized indirectly through the perivertebral membrane.

Intrathecal baclofen pump implantation for complex regional pain syndrome in a patient with a spinal cord stimulator: consideration about optimal location of intrathecal catheter tip - A case report -

고명진,이현성,조현지,김성록,전상윤,이상은 대한마취통증의학회 2018 Anesthesia and pain medicine Vol.13 No.2

Intrathecal baclofen (ITB) pump implantation can be used to control dystonia and severe pain associated with complex regional pain syndrome (CRPS) with or without a spinal cord stimulator (SCS). A 45-year-old female patient had gotten an SCS to control the pain of CRPS. However, she suffered from chronic intractable pain in her left ankle and foot despite paresthesia in the entire painful area because the effectiveness of the SCS gradually diminished over time. In a trial of intrathecal drug administration, baclofen was superior to morphine for pain relief, had fewer side effects, and was superior in terms of patient satisfaction. To achieve the greatest degree of pain relief from the ITB pump, the tip of the intrathecal catheter was carefully placed in relation to the SCS. Over a oneyear follow-up period, the patient experienced mild pain without any adverse effects.