생쥐 난자 및 초기배아에서 Leptin 수용체 발현 및 Leptin에 의한 Mitogen Activated protein Kinase 활성의 조절 및 난자의 성숙 조절

강병문,한현주,서혜영,홍석호,계명찬,Kang, Byung-Moon,Han, Hyun-Joo,Seo, Hye-Young,Hong, Suk-Ho,Gye, Myung-Chan 대한생식의학회 2001 Clinical and Experimental Reproductive Medicine Vol.28 No.2

Objective: To verify the expression of leptin receptor (OB-R) in oocytes and preimplantation embryos, the involvement of mitogen activated protein kinase (MAPK or Erk1/2) in the leptin signaling, and effect of leptin on the oocyte maturation in mice. Method: RT-PCR analysis of OB-R was conducted in germinal vesicle (GV)-intact and MII stage oocytes, and 1, 2, 8-cell embryos and blastocysts. Germinal vesicle breakdown (GVB), polar body extrusion, monitored in the presence or absence of leptin ($1{\mu}M$). Following the leptin treatment, temporal changes in MAPK activity were verified by immunoprecipitation and in vitro kinase assay in MII oocytes. Results: The expression of OB-R mRNA was found in GV and MII oocyte but not in the embryos. MAPK activity of the MII oocytes was significantly increased by brief incubation in the HTF supplemented with leptin ($1{\mu}M$). Priming of PD098059, a MEK inhibitor to leptin treatment attenuated the activation of MAPK by leptin in MII oocytes. Following 24 hrs of culture of the GV oocytes, leptin significant increased the GVB and 1 st polar body extrusion. Conclusion: This result suggested that functional interaction between leptin and OB-R resulted in potentiation of MAPK (Erk1/2) activity in MII oocytes through MEK activation and that leptin might be a local regulator of meiotic maturation of the mouse oocytes.

난관복원술전에 실시한 불임검사의 임신율 예측지표로서의 유용성에 관한 연구

강병문,Kang, Byung-Moon 대한생식의학회 1993 Clinical and Experimental Reproductive Medicine Vol.20 No.1

Since the successful advent of IVF-ET, reproductive surgeons have been forced to compare pregnancy outcomes of surgical procedures for tubal infertility with those of IVF-ET. The current study was designed in an effort to determine the usefulness of pre-operative infertility work-up, especially diagnostic laparoscopy, in predicting the pregnancy rate and in assessing the reversal feasibility. 109 patients who underwent a microsurgical tubal reversal were reviewed. The patients were followed over one year. The total intrauterine pregnancy, spontaneous abortion, and ectopic pregnancy rates were 66.4%, 3.7%, and 7.3%, respectively. The result showed that the use of loupe might increase the chance of the ectopic pregnancy. I found that the final tubal length, especially the longest tubal length, the site of reanastomosis, and the methods of sterilization affected the pregnancy rates. A 85% intrauterine pregnancy rate was achieved in the patients with good preoperative assessments(Group II-1). There was only 10% incidence of intrauterine pregnancy in the patients with bad assessments(Group II-2). It is concluded that the pre-operative infertility work-up, especially diagnostic laparoscopy, is a good diagnostic tool in assessing the reversal feasibility.

흰쥐 자궁 상피와 내막에서 기원한 세포주의 체외배양

강병문,이석원,채희동,강은희,추형식,김정훈,장윤석,남주현,Kang, Byung-Moon,Lee, Suk-Won,Chae, Hee-Dong,Kang, Eun-Hee,Chu, Hyung-Sik,Kim, Chung-Hoon,Chang, Yoon-Seok,Nam, Joo-Hyun 대한생식의학회 1999 Clinical and Experimental Reproductive Medicine Vol.26 No.1

Since the blastocyst is broken and spreads out on a flat plastic culture dish (two dimensional culture) during in vitro development, it has been difficult to study the implantation process. It also has been difficult to analyse the interactions between endometrial epithelial and stromal cells because of the lack of a long-term in vitro model which can stimulate in vivo characteristics, as these cells eventually fail to proliferate or cease to express differentiated functions. Recently nontransformed cell lines, CUE-P and CUS-V2, derived from rat endometrial epithelium and stroma were reported. In this study, morphology of CUE-P and CUS-V2 was examined and oxytocin gene expression by CUE-P cells was demonstrated by RT-PCR. The CUE-P cells have a cuboidal morphology and CUS-V2 cells resemble fibroblast and exhibit a spindle-like morphology. In RT-PCR, same size of PCR products of oxytocin gene at hypothalamus, uterus and CUE-P cells were demonstrated. These results showed three dimensional culture system could be made by using the new cell lines.

배아밀도와 배양액 용량이 착상전후의 생쥐배아의 체외 성장에 미치는 영향

강병문,전용필,김지영,김정희,이지윤,채희동,김정훈,장윤석,목정은,Kang, Byung-Moon,Cheon, Yong-Pil,Kim, Ji-Young,Kim, Jeong-Hee,Lee, Ji-Yun,Chae, Hee-Dong,Kim, Chung-Hoon,Chang, Yoon-Seok,Mok, Jung-Eun 대한생식의학회 1997 Clinical and Experimental Reproductive Medicine Vol.24 No.3

The effects of embryo number and incubation volume on the development of mouse embryos were evaluated. The growth rate of two-cell mouse embryos to attached blastocyst stage and the growth rate of blastocysts to early somite stage were assessed after culture in different incubation volumes and embryo densities. Embryos were collected from ICR female mice superovulated with pregnant mare serum gonadotropin and human chorionic gonadotropin and mated by ICR males. In experiment 1, groups of one, five, ten, twenty 2-cell embryos were cultured in 10-, 50-, 500-, 1000-${\mu}l$ drops of BWW media under mineral oil at $37^{\circ}C$ in a humidified atmosphere of 5% $CO_{2}$ and 95% air. As the incubation volume decreased, significantly (p<0.05) higher rates of embryos reached morular and blastocyst stage on day 3 and 4 culture, respectively. In experiment 2, groups of one, five, ten, twenty blastocysts were cultured in 1- and 2-ml volumes of CMRL 1066 media under same condition as in experiment 1. However the reverse was the result. Decreasing the number of embryos incubated per volume from 1 to 20 significantly (p<0.05) increased the number of blastocysts reaching the late egg cylinder (LEC) and early somite (ES) stage on day 6 and 8 culture, respectively, regardless of incubation volume. Blastocysts cultured in 2ml had higher (p<0.05) development rates to LEC and ES stage on day 6 and 8 culture, respectively, than embryos cultured in 1ml. Our results suggest that the effects of embryo number and incubation volume on the development of mouse embryos are stage specific and the shifting point was between hatching and EEC stage.

Extracellular Matrix 배지에서 생쥐 배아의 발생 및 아폽토시스

강병문,손인표,정병목,최규완,계명찬,Kang, Byung-Moon,Sohn, In-Pyo,Chung, Byung-Mok,Choi, Kyoo-Wan,Gye, Myung-Chan 대한생식의학회 2000 Clinical and Experimental Reproductive Medicine Vol.27 No.3

Objective: To verify the effect of Matrigel, a ECM complex from Engelbreth-Holm-Swarm (EHS) mouse sarcoma on the preimplantation development and apoptosis of mouse fertilized eggs. Method: Late pronucleus stage eggs were cultured through the blastocyst stage in the presence of Matrigel (0.5%, v/v). Characteristics of apoptosis and cell number assesed by Hoecst staining and TUNEL labeling at the blastocyst stage, respectively. Results: Morphological development, number of cells per embryo was significantly increased but rate and number of TUNEL positive nuclei of the embryo were decreased in the presence of Matrigel. Conclusion: This result suggested that at low concentration of Matrigel improves both viability and morphological development in the preimplantation mouse embryos.

구원의 서정과 성경적 상담의 통합에 대한 이론적 고찰

강병문(Kang, Byung-Moon) 한국개혁신학회 2010 개혁논총 Vol.16 No.-

성도들의 삶이 예수그리스도의 구속의 완성 즉 죽으심과 부활의 역사적 구속적 사실을 삶 가운데 적용하는 구원의 적용이라면, 성경적 상담은 당연히 구원의 적용을 도와 성도로 하여금 하나님의 총체적 은혜가 무엇이며, 그리스도 중심의 하나님과 함께 하는 삶이 어떤 순서와 내용으로 진행되며, 그것에 대해서 우리가 어떤 분명한 이해를 가지고 주님을 닮는 성숙한 삶을 살아갈 것인가를 알게 하는 통로가 되어야할 것이다. 그런고로 먼저 개혁주의 입장에서의 공통성이 있지만 다양한 구원의 서정을 여러 측면에서 파악하고 구원의 서정과 연결되는 다양한 성경적 파라다임을 가진 성경적 상담의 제이론들을 총체적으로 연결 시켜 살펴보아 신학적 측면에서의 신앙 성숙에 따른 원리와 내용을 상세히 알뿐아니라 그것에 따른 인간적 성숙내지는 예수그리스도를 닮아가는 성화의 이론과의 상관 관계를 밝혀 전인적 인간의 구원에 따른 성도의 그리스도 안에서의 전인적 발달 과정을 총체적으로 이해하려 시도하였다. As the Biblical counseling theories actually are application of salvation which applies the accomplished work of redemption, that is, the redemptive historical acts of death and resurrection of Jesus Christ to everyday lives of the saints, they need to know clearly what the total grace God provided in Christ is, and what the order and contents of lives living together with God are in following the order of salvation applied for the mature Christian living. Following results are found through this article. First of all, there are several ways to explain the way of salvation(ordosalutis) even in the reformed tradition, which has to be sought and studied, even though they have the common ground as a reformed tradition. Secondly, In Christ the whole person development, the person which is made in the image of God is tried to be understood in a whole manner according to the way of salvation. Finally, the relationship between the ordo salutis and the Biblical counseling are searched and discussed. In the light of the ordo salutis in reformed theological tradition, Biblical counselings are analyzed and reinterpreted.

한국 폐경 여성에서 운동이 골밀도에 미치는 영향

강병문 ( Byung Moon Kang ),김미란 ( Mee Ran Kim ),윤병구 ( Byung Koo Yoon ),이병석 ( Byung Seok Lee ),정혜원 ( Hye Won Chung ),최훈 ( Hoon Choi ),박형무 ( Hyoung Moo Park ),김정구 ( Jung Gu Kim ) 대한폐경학회 2007 대한폐경학회지 Vol.13 No.2

연구목적: 한국 폐경 여성에서 운동이 골다공증이나 골감소증의 위험도에 미치는 영향에 대해서 조사하였다. 연구재료 및 방법: 폐경 여성 1,105명을 대상으로 이중 에너지 X선 흡수 계측기를 이용하여 요추와 대퇴골의 골밀도를 측정하였으며 대상 폐경여성들의 나이, 월경력, 신장, 체중 등을 조사하였다. 대상자에게 운동에 관하여 자가 기입식으로 설문지를 조사하였다. 결과: 하루에 걷는 거리가 1 km 이상인 군과 미만인 군으로 구분하여 비교한 결과 요추와 대퇴부의 골다공증 및 골감소증 위험도가 덜 걷는 군에서 30% 정도 증가하였다. 하루에 오르는 계단의 수가 20개 이상인 군과 미만인 군으로 나누어 비교한 결과 통계학적인 의의는 없었지만 요추와 대퇴부의 골다공증 및 골감소증 위험도가 계단수가 적은 군에서 증가하는 경향을 보였다. 일주일간에 운동시간이 2시간 이상인 군과 1시간 미만인 군으로 구분하여 비교한 결과 요추와 대퇴부의 골다공증 및 골감소증 위험도가 운동 시간이 적은 군에서 40∼50% 정도 증가하였다. 나머지 설문 문항중에서는 유의한 결과가 없었다. 결론: 운동은 골밀도에 긍정적인 영향을 주어 골소실 방지나 골량의 유지에 도움이 되는 것으로 보이며 그 효과는 운동강도나 크거나 운동시간이 길수록 더욱 높아지는 것으로 사료된다. Objectives: To determine relationship between exercise and bone mineral density of lumbar and femur sites in postmenopausal women. Methods: A cross-sectional study in 1105 Korean menopausal women, mean+SD age 55.8+7.0 years, all healthy and free of medications affecting bones, including estrogen. We explored physical activity and bone mineral density in postmenopausal women Bone mineral density (BMD) of lumbar and femur regions and body composition were measured by dual X-ray absorptiometry. Types, duration and other variable measures of exercise were assessed by questionnaire. Results: A group of postmenopausal women with more than 1 km daily walking showed 30% less risk of osteopenia or osteoporosis than women with less than 1km. A group of postmenopausal women with more than 20 step climbing showed tendency of less risk of osteopenia or osteoporosis than women with less than 20. A group of postmenopausal women doing exercise more than 2hours/week showed 40-50% less risk of osteopenia or osteoporosis than women doing exercise less than 1hour/week. Other variable measures of exercise did not show any statistical difference. Conclusion: The results support the notion that higher intensity and longer duration of exercise is more effective for bone sparing than lower intensity and shorter duration of exercise.

한국 의사들의 WHI 이해와 여의사들의 호르몬치료 순응도

강병문 ( Byung Moon Kang ),이향아 ( Hyang Ah Lee ),이사라 ( Sa Ra Lee ),김미란 ( Mee Ran Kim ),박형무 ( Hyoung Moo Park ),윤병구 ( Byung Koo Yoon ),이병석 ( Byung Seok Lee ),정혜원 ( Hye Won Chung ),조수현 ( Soo Hyun Cho ),최훈 ( 대한폐경학회 2005 대한폐경학회지 Vol.11 No.3

폐경여성에서 승마추출물과 St. John`s wort 복합제의 고용량 치료법에 따른 유효성과 부작용에 관한 연구

강병문 ( Byung Moon Kang ),이사라 ( Sa Ra Lee ),구윤희 ( Yun Hee Goo ),김성훈 ( Sung Hoon Kim ),채희동 ( Hee Dong Chae ),김정훈 ( Chung Hoon Kim ) 대한폐경학회 2006 대한폐경학회지 Vol.12 No.4

폐경증상과 보완대안 요법

강병문 ( Byung Moon Kang ) 대한폐경학회 2007 대한폐경학회지 Vol.13 No.3

Many postmenopausal women are seeking to use complementary and alternative therapy for management of menopausal symptoms because the Women`s Health Initiative influenced many women to discontinue hormone therapy. The purpose of this article is to assess the effectiveness of complementary and alternative therapy in the management of menopausal symptoms. This review is based on the randomized controlled trials and meta-analysis comparing a complementary and alternative therapy with placebo or control for treatment of menopausal symptoms. Studies of phytoestrogens and other biologically based agents showed mixed results. Studies using mind-body, energy, manipulative and body based therapies and whole medical systems showed little benefits in treating menopausal symptoms. Data are insufficient to support the effectiveness of any complementary and alternative therapy in this review for the management of menopausal symptoms.