Induction of erythroleukaemia by F - MuLV and Purification of Erythropoietin

김정곤 한국유전학회 1986 Genes & Genomics Vol.8 No.1

Friend Murine Leukemia Virus (F-MuLV) is affective against 2-day old new borns in NFS/n Swiss mice. Infected babies suffer from splenomegaly and anemia at the age of 4-5 weeks beyond which the condition worsens and is fatal. However, the spleen size can be reduced by hypertransfusion for 8 weeks. If left alone after this treatment, the spleen enlarges and becomes palpable again. This time the spleen cells are transplantable and inflict the same disease if injected to normal adults. While these leukemic cells were considered to be hormone-independent, addition of erythropoietin (EPO) was found to be essential for maximum growth in culture. Influence of EPO on the in vivo growth of leukemic cells was indicated by our findings that administration of the hormone caused a more severe leukemia and rapid death while, transfusion of red blood cells, which lowers endogenous EPO, led to increased survival of leukemic mice. It is suggested that hormone-associated therapy may be efficacious in the treatment of this and perhaps, other leukemias. Several erythroleukemic cell lines released factors which stimulated erythropoiesis in vivo and in vitro. A simple five step scheme has been devised which allows purification of this erythropoietic activity to apparent homogeneity. The methods employed included lectin affinity chromatography (wheat germ agglutinin), gel filteration (ultro gel ACA 44), ion exchange, hydroxylapatite, and high performance liquid chromatography, Following polyacrylamide gel electrophoresis, biological activity was recovered in an area corresponding to a molecular weight of 35,000 daltons. Silver staining of a polyacrylamide gel after electrophoresis of our most purified preparation revealed a single band of 35,000 daltons.

Morphological Oogenesis in the otu1 Mutant of Drosophila melanogaster Meigen (Diptera: Drosophilidae)

김정곤,정계준 한국곤충학회 2002 Entomological Research Vol.32 No.3

The sex-linked, recessive otu1 mutant of Drosophila melanogaster was studied cytologically to reveal the effect of the otu gene mutation on oogenesis. Consequently, the germarium of the otu1 mutant was found to be larger as compared to the normal fertile one and contains many undifferentiated tumor cells resembling cystocytes. Each ovary consists of three types of ovarioles; ovarioles with only a germarium, ovarioles with a germarium and one tumor chamber, and ovarioles with a germarium and two tumor chambers. In the otu1 germarium, the mesodermal follicle cells invade the clusters of different number of tumor cells at the posterior region of the germarium in order to make the tumor chamber and the dividing frequency of the tumor cells in the .rst tumor chamber was six times more than in the second tumor chamber. Follicle cells surrounding the tumor chamber are morphologically normal, but the number of the follicle cells becomes less as the tumor chambers move down contrary to the fertile one. The otu1 mutant female never lays the egg and the formation of the vitelline membrane is incomplete. The otu1 mutant tumor cells also showed characteristics of certain rapidly dividing cells and its nucleus is lobulated.

국가위기관리기본지침 개정과 국가위기관리 매뉴얼 개선

김정곤,정종수,김태환,김희철 한국재난정보학회 2018 한국재난정보학회 학술대회 Vol.2018 No.11

최근 정부는 국가위기관리기본지침을 개정하고 국가 재난안전 관리 체계를 강화하기 위한 세부적인 작 업을 진행하고 있다. 주요 내용으로는 청와대의 컨트롤타워 역할 정립, 비상대응 기구 간 권한과 책임의 명확화, 위기경보 발령체계 개선 등으로 다양한 내용을 포함하고 있다. 한편 이러한 내용을 실무에 반영하 기 위해서는 국가 위기관리 매뉴얼(표준, 실무, 행동)의 개정을 통해 가능하다. 본 논문에서는 개정된 국가위기관리기본지침의 개정 주요내용과 매뉴얼 개정방향에 대한 소개 및 개정 내용에 대한 검토를 통해 재난현장에서 행동매뉴얼의 작동성 및 효율성 개선 효과에 대하여 고찰하였다. 이번 국가위기관리기본지침 개정을 통해 각 기관의 재난관리 임무와 책임을 명확히 한것은 매뉴얼의 작동 성 강화와 현장 업무의 효율성의 문제를 상당부분 개선 할 것으로 기대된다. 그러나 매뉴얼 간의 논리적 연결 관계에 대한 불명확성이 여전히 존재하고. 상위기관의 재난현장 지휘에 대한 의사결정 관여 수준에 대해서는 현장지휘에 대한 혼선을 주지 않는 수준 결정을 위한 검토가 필요할 것으로 사료된다.

실무교육을 중심으로 한 건축설계 교육방향에 관한 연구 ( Education Development in Architectural Design )

김정곤 대한건축학회 2002 建築 Vol.46 No.4

ESG경영선도 종합환경전문공기업 대구환경공단 경영혁신 성과 및 계획

김정곤 한국행정학회 2022 한국행정학회 학술발표논문집 Vol.2022 No.1

우치무라 간조(內村鑑三)의 양명학 이해

김정곤 한국양명학회 2013 한국양명학회 학술대회 논문집 Vol.2013 No.5

An Empirical Study on Effects of Korea’s Cultural Exports

김정곤,안세영 한국무역학회 2012 Journal of Korea trade Vol.16 No.2

This paper aims to find how Korea’s cultural exports, a measure of cultural proximity, influence its total exports. Centered on consumption goods, detailed product-by-product analyses are also conducted. Empirical analyses using the gravity model show that a 100% increase of Korea’s cultural exports has the effect of increasing total exports by 3.9-4.7%; the export increasing impact of reproducible cultural goods is 3.8-6.1%. The one-year lagged cultural exports also significantly affect the current total exports. Although the overall positive impact of cultural exports is stronger to those of nondurable consumer goods, exports of some durable goods are also positively influenced by them. Meanwhile, the positive impact of cultural exports is larger in higher-income nations. In the case of higher-income countries, the export-increasing effect is larger on durable consumer goods, but, in the case of lower-income countries, larger on nondurable consumer goods. Asian countries are more positively affected by Korea’s cultural exports than those in the other regions. From the viewpoint of trade policy, Korea should take greater interest in cultural exports to higher-income countries in order to increase their positive impact.

전통시장 화재 발생 및 확대 위험요인에 관한 연구

김정곤,박창일,정재욱,김성곤,Kim, Jung-Gon,Park, Chang-Il,Jung, Jae-Wook,Kim, Seong-Gon 한국재난정보학회 2021 한국재난정보학회 논문집 Vol.17 No.1

Purpose: Traditional markets often have irregular space utilization patterns because the spaces are created and divided as time passed. Internally, there is high risk of fire due to problems such as aging facilities and high-density of stores and externally, there is high risk of fire spread since it is often adjacent to deteriorated residential and commercial facilities. Method: In this paper, on-site investigations were carried out to check fire risk factors and fire spread risk, and fire occurrence and expansion risk factors were investigated for traditional markets in Hwanghak-dong and Dong-daemun by using large-scale fire data from existing traditional markets. Result: As a result of the analysis, there are likely to be various problems such as high fire load and lack of safety awareness due to aging facilities and high-density of stores. In particular, it is necessary to prepare countermeasures because deteriorated residential facilities with narrow alleys around traditional markets have high fire spread. It is situation that while traditional markets mainly are managing for fire and disaster centering on the merchant association, the surrounding residential areas are not properly managed. Conclusion: It is necessary to manage deteriorated residential facilities with traditional markets, also to be linked early warning system and information to evacuate rapidly in case of fire there. 연구목적: 전통시장은 시간적인 차이를 두고 필요에 의하여 공간이 생성 및 구획되어 불규칙적인 공간 활용 패턴을 갖는 경우가 많다. 그 영향으로 내적으로는 시설노후화, 점포의 과밀화 등과 같은 문제로 화재발생 위험성이 높고, 외적으로는 노후 주거 및 상업 시설과 인접한 경우가 많아 화재확산의 위험성이 높다. 연구방법: 본 논문에서는 기존 전통시장 대형화재 자료를 활용하여 황학동과 동대문 일대의 전통시장을 대상으로 화재 위험요인 및 화재 확산 위험성에 대하여 현장조사를 실시하고 화재 발생과 확대 위험요인에 대해 검토하였다. 연구결과: 분석결과, 노후화시설과 점포과밀화로 인한 높은 화재하중 및 안전의식 부재 등과 같은 다양한 문제점이 존재하는 것으로 파악되었다. 특히, 전통시장주변의 좁은 골목과 함께 발달한 노후주거시설은 화재확산 위험성이 매우커서 대책마련이 필요하다. 전통시장은 상인회를 중심으로 화재 및 재난관리를 하는 반면 주변 주거지역은 제대로 관리되고 있지 못하였다. 결론: 전통시장과 함께 주변의 노후주거시설을 함께 관리할 필요가 있으며, 전통시장과 노후주거시설에서 발생한 화재에 대하여 신속한 대피가 가능하도록 조기경보체계와 정보연계가 필요하다.

Two newly recorded genera Tolongia and Yamatolygus of the mirine plant bugs (Hemiptera: Heteroptera: Miridae) from the Korean Peninsula

김정곤,WonGun Kim,정성훈 국립중앙과학관 2019 Journal of Asia-Pacific Biodiversity Vol.12 No.1

Two genera of the plant bug in the subfamily Mirinae (Hemiptera: Heteroptera: Miridae) are newlyreported from the Korean Peninsula with discoveries of Tolongia pilosa Yasunaga, 1991 and Yamatolygusinsulanus Yasunaga, 1994. The male genital structure of T. pilosa is remarked. Female genitalia ofY. insulanus is described for the first time. Morphological information such as diagnoses is also provided.

都評議使司의 機能

金玎坤 진주교육대학교 1968 論文集 Vol.2 No.1