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산란계의 연령과 면역주기에 따른 난황 중의 Streptococcus mutans 특이 항체 함량
성기승,김영붕,노정해,이남형,한찬규,손동화 한국축산학회 1999 한국축산학회지 Vol.41 No.5
Streptococcus mutans-specific IgY content change, laying rate, egg weight and weight change were measured for 17-week and 30-week old hens. Vaccinations with Streptococcus mutans were made two times(eight week interval), three times(four week interval) and five times(two week interval), respectively. It was observed that the laying rate of vaccinated hens was likely lower than that of non-vaccinated group. No effect on body weight by vaccination was found out. Egg weight did not show a certain tendency by vaccination. Anti-S. mutans IgY started to be detected two weeks after the lst vaccination for 30-week old hens. It was not detected for non-vaccinated group. The antibody activity was consistently detected after 8 weeks from the last vaccination. The measurement of total IgY and S. mutans-specific IgY in the egges from vaccinated hens revealed that IgY tended to increase with the number of vaccination. S. mutans-specific IgY content of five-time vaccinated l7-week hens was much higher than that of 30-week old hens. To obtain steady amount of specific IgY, multiple vaccination with two week interval was recommended.
난황 중의 항-Salmonella gallinarum 특이 항체 생성 및 가공 특성
노정해,김미현,김영붕,성기승,이남형,Rho, J.H.,Kim, M.H.,Kim, Y.B.,Sung, K.S.,Lee, N.H. 한국축산학회 2005 한국축산학회지 Vol.47 No.4
Immunization of layers against Salmonella gallinarum(S.G.) which causes fowl typhoid resulted in production of anti-S.G. IgY rich eggs. Water soluble fraction was obtained from egg yolk using various gum solutions such as 0.1%(Sigma C-3889) λ-carrageenan; 1% and 2% cold water soluble carrageenan; 1% and 2% hot water soluble carrageenan; and 1% cold water soluble carrageenan with 1% hot water soluble carrageenan. Among them, λ-carrageenan 0.1% treatment showed a high recovery rate, possessing high IgY contents. In the range of pH 5-9, more than 70 percent of IgY was existent. Moreover, Anti-S.G. IgY was relatively heat-stable. This study revealed that immunoglobulin against fowl typhoid could be produced successfully by layers and the IgY was sustainable to further processing due to its pH and heat stability. IgY is promising to be utilized for prevention and treatment of fowl typhoid in industrial scale. 가금티푸스 유발균인 Salmonella gallinarum 대항하는 항체의 함량이 높은 계란을 생산하기 위해 면역처리 후 산란계의 난황에서 항체를 분리하여 총 IgY와 특수 IgY의 함량의 변화와 그 특성을 조사하였다. 먼저 난황으로부터 항체의 수율과 경제적인 측면을 비교하기 위하여 난황 분리된 난황 25 g을 λ-카라기난(Sigma C-3889) 0.1%, 냉수 가용성(MSC #10387) 1%, 2%, 90℃ 가용성 (MSC #10386) 1%, 2% 및 냉수 가용성(0.1%) : 90℃ 가용성(0.1%) = 1 : 1 1%, 2%로 사용하여 분리하는 하였을 때 λ-카라기난 0.1%, 냉수 가용성 1% 비교적 높은 회수율과 total IgY, specific IgY 함량을 나타내었지만 λ-카라기난 0.1%가 더 나은 것으로 나타났다. Specific IgY는 마지막 백신 접종 후 44주간까지 꾸준히 검출되고 있는 것으로 나타났다. 산도 변화에 의한 total IgY의 잔존 함량을 보면 pH 5-9 범위에서 70% 이상 잔존하며, specific IgY도 pH 4-10에서 74% 이상 존재하여 산에 대하여 대체로 안정적임을 알 수 있었다. 열처리 후 조항체 용액을 total IgY, specific IgY 역가 높게 유지되는 것을 관찰 할 수 있었다. 이로써 난황으로부터의 면역 항체 생산의 가능성이 확인되었고 이러한 IgY는 추출과 가공처리 등에 의해 비교적 안정적인 것으로 나타나 IgY를 이용한 가금티푸스의 예방 또는 치료용의 대량 생산 가능성이 높은 것으로 사료되었다.
梁仁淑,成老貞,崔明周,兪映旭,李垠定,崔江姬,李光子,孫仁子 한국병원약사회 1988 병원약사회지 Vol.5 No.2
It is considered as the important roles of pharmacists to review prescriptions before starting dispensing. We have analyzed the details of telephone or written inquiries on the prescriptions collected from January, 1986 to December, 1986 for one year. Frequency of inquiries was about 1.1% or 410 prescriptions per month, (4925 out of 453,358 prescriptions from inpatients and outpatients). The details of the inquiries were dosage (28.6%), drug name(27.1%), strength(13.2%), insufficient writting of presciption to the rules(8.3%), administration schedule, dosage form, period of medication and so on. As to the writing errors of drug names which are most frequently occur, the causes were also analyzed. As a result of the review we can be concluded that it is desirable to educate doctors about prescription writing to decrease prescription errors.
여러가지 산란계 사료 첨가제가 계란의 IgY 수준과 산란율에 미치는 영향
이남형,노정해,한찬규,성기승 한국축산학회 1999 한국축산학회지 Vol.41 No.2
Breeding tests were performed in order to obtain high IgY eggs from laying hens by producing 5 specially formulated feeds(supplemented with 4% garlic powder, 2% or 4% Kelp meal, 2% sea tangle powder or Se(0.5 ppm) + Vit. E(300% NRC standard). Supplemients tended to decrease laying rates. The result of average IgY contents in the whole experimental period showed that the 4% kelp meal treated group had much higher IgY value than the other groups. Especially, the result of the last week revealed that only the 4% kelp meal treated group and 2% sea tangle treated group had higher values than the control group. The IgY value of the 4% kelp treated group was higher than that of the control group by 10%, and the increase was presumed to be manifested by extending the experimental period. To increase the total IgY content, another breeding test was performed with three supplements(2% ginger, 0.5% cinnamon, or 2% mint). A high IgY content was detected for cinnamon and mint-added groups without decreasing the of laying rates.