Fenofibrate Increases Radiosensitivity in Head and Neck Squamous Cell Carcinoma via Inducing G2/M Arrest and Apoptosis

Liu, Jia,Ge, Yang-Yang,Zhu, Hong-Cheng,Yang, Xi,Cai, Jing,Zhang, Chi,Lu, Jing,Zhan, Liang-Liang,Qin, Qin,Yang, Yan,Yang, Yue-Hua,Zhang, Hao,Chen, Xiao-Chen,Liu, Zhe-Ming,Ma, Jian-Xin,Cheng, Hong-Yan,S Asian Pacific Journal of Cancer Prevention 2014 Asian Pacific journal of cancer prevention Vol.15 No.16

Radiation therapy is an important treatment for head and neck squamous cell carcinoma (HNSCC). However, how to promote radiation sensitivity in HNSCC remains a challenge. This study aimed to investigate the radiosensitizing effects of fenofibrate on HNSCC and explore the underlying mechanisms. HNSCC cell lines CNE-2 and KB were subjected to ionizing radiation (IR), in the presence or absence of fenofibrate treatment. Cell growth and survival, apoptosis and cell cycle were evaluated. In addition, CNE-2 cells were xenografted into nude mice and subjected to IR and/or fenofibrate treatment. The expression of cyclinB and CDK1 was detected by Western blotting. Our results showed that fenofibrate efficiently radiosensitized HNSCC cells and xenografts in mice, and induced apoptosis and G2/M arrest via reducing the activity of the CDK1/cyclinB1 kinase complex. These data suggest that fenofibrate could be a promising radiosensitizer for HNSCC radiotherapy.

중국 중학생이 지각한 부모 및 교사의 자율성지지, 기본심리욕구, 사회정서역량 간의 관계 연구

( Yang Yue ),신태섭 ( Tae Seob Shin ) 인하대학교 교육연구소 2021 교육문화연구 Vol.27 No.1

본 연구의 목적은 자기결정성이론에 기반하여 부모 및 교사의 자율성 지지가 기본심리욕구를 매개로 중국 청소년의 사회정서역량에 미치는 영향을 살펴보는 데 있다. 이를 위해 중국 산동성 쯔보시 중학교 3학년 434명의 데이터를 사용하여 부모 및 교사의 자율성 지지와 중학생의 사회정서역량 간의 관계에서 중학생의 기본심리욕구의 매개효과를 확인하였다. 본 연구의 주요 결과는 다음과 같다. 첫째, 부모의 자율성 지지가 교사의 자율성 지지보다 중학생의 기본심리욕구 및 사회정서역량에 더 큰 영향을 미치는 것으로 나타났다. 둘째, 중학생의 기본심리욕구가 충족될수록 중학생의 사회정서역량 수준이 높아지는 것으로 나타났다. 셋째, 중학생이 지각한 부모 및 교사의 자율성 지지가 많을수록 중학생의 사회정서역량 수준이 높아졌다. 특히 중학생이 지각한 부모의 자율성 지지는 교사의 자율성 지지보다 중학생의 사회정서역량에 더 직접적인 영향을 주었다. 넷째, 부모와 교사의 자율성 지지는 중학생의 기본심리욕구를 충족시켜 사회정서역량에 긍정적 영향을 미쳤다. 이때 역시 부모의 자율성 지지는 교사의 자율성 지지보다 중학생의 기본심리욕구를 더 충족시키고 사회정서역량에 보다 큰 영향을 주는 것으로 나타났다. 연구 결과는 중학생의 사회·정서적 역량을 개발하기 위해 교사뿐만 아니라 부모의 역할이 필수적이고, 특히 중국에서 부모의 사회정서학습에 대한 인식 개선과 부모 교육 확대의 필요를 시사한다. Purpose of this study was intended to examine the effect of parent and teacher's autonomy support on adolescent's social and emotional competencies, focusing on the mediating effect of basic psychological needs based on self-determination theory. Data of 434 students in Zibo city in Shandong Province were used in the analysis of a structural equation modeling. The main results of this study are as follows. First, the more basic psychological needs of middle school students they were met, the higher level of social and emotional competencies of middle school students have. Second, the more parents and teachers' autonomy supported middle school students perceived, the higher level of social and emotional competencies of middle school students they had. In particular, parental autonomy support perceived by students had a more direct impact on the social and emotional competencies. Lastly, parents' autonomy support stimulated middle school students' basic psychological needs more than teachers' autonomy support, and has a greater influence on social and emotional competencies of middle school students. In conclusion, in order to develop the social and emotional competencies of middle school students, the role of parents as well as teachers is essential, suggesting the need to raise awareness of parent education in China and to expand and implement parent education at national level.

Critical effects of long non-coding RNA on fibrosis diseases

Yue Zhang,Gang Luo,Yi Zhang,Mengjie Zhang,Jian Zhou,Weiwu Gao,Xiuyun Xuan,Xia Yang,Di Yang,Zhiqiang Tian,Bing Ni,Jun Tang 생화학분자생물학회 2018 Experimental and molecular medicine Vol.50 No.-

The expression or dysfunction of long non-coding RNAs (lncRNAs) is closely related to various hereditary diseases, autoimmune diseases, metabolic diseases and tumors. LncRNAs were also recently recognized as functional regulators of fibrosis, which is a secondary process in many of these diseases and a primary pathology in fibrosis diseases. We review the latest findings on lncRNAs in fibrosis diseases of the liver, myocardium, kidney, lung and peritoneum. We also discuss the potential of diseaserelated lncRNAs as therapeutic targets for the clinical treatment of human fibrosis diseases.

Metabonomics study on the hot syndrome of traditional Chinese medicine by rapid resolution liquid chromatography combined with quadrupole time-of-flight tandem mass spectrometry

Yang Wang,Li M,Yi Sun,Liman Yang,Hao Yue,Shuying Liu 대한약학회 2014 Archives of Pharmacal Research Vol.37 No.7

The hot syndrome refers to any feverish conditionsduring a pathological development, a sub-healthphenomenon, and is a potential risk for human health. Themetabonomics study on the hot syndrome may provideinsight into understanding of its pathology and play a rolein the prevention and treatment of its related diseases. Inthis paper, the rats were dosed with the hot syndromeprescription, ginseng and water. The corresponding urinesamples were identified by rapid resolution liquid chromatographycombined with quadrupole time-of-flight tandemmass spectrometry. More than 1,000 metaboliccompounds from different urine samples could be furtherdifferentiated by principal component analysis. As a result,the rat body temperature and weight were recognized as thehot syndrome related factors. Some specific metaboliteshave been discovered as a pattern of the potential biomarkers for the hot syndrome. The results showed thatginseng cannot cause the hot syndrome in a reasonabledose, but the hot syndrome prescription can. It is suggestedthat ginseng cannot be used only as a tradition Chinesemedicine but also as a nutrient. The work showed metabonomicsmethod is a valuable tool in studying mechanismof the hot syndrome.

A Novel Phase Locked Loop for Grid-Connected Converters under Non-Ideal Grid Conditions

Yang, Long-Yue,Wang, Chong-Lin,Liu, Jian-Hua,Jia, Chen-Xi The Korean Institute of Power Electronics 2015 JOURNAL OF POWER ELECTRONICS Vol.15 No.1

Grid synchronization is one of the key techniques for the grid-connected power converters used in distributed power generation systems. In order to achieve fast and accurate grid synchronization, a new phase locked loop (PLL) is proposed on the basis of the complex filter matrixes (CFM) orthogonal signal generator (OSG) crossing-decoupling method. By combining first-order complex filters with relation matrixes of positive and negative sequence voltage components, the OSG is designed to extract specific frequency orthogonal signals. Then, the OSG mathematical model is built in the frequency-domain and time-domain to analyze the spectral characteristics. Moreover, a crossing-decoupling method is suggested to decouple the fundamental voltage. From the eigenvalue analysis point of view, the stability and dynamic performance of the new PLL method is evaluated. Meanwhile, the digital implementation method is also provided. Finally, the effectiveness of the proposed method is verified by experiments under unbalanced and distorted grid voltage conditions.

Biomechanical Evaluation of 2 Endoscopic Spine Surgery Methods for Treating Lumbar Disc Herniation: A Finite Element Study

Yang Zou,Shuo Ji,Hui Wen Yang,Tao Ma,Yue Kun Fang,Zhi Cheng Wang,Miao Miao Liu,Ping Hui Zhou,Zheng Qi Bao,Chang Chun Zhang,Yu Chen Ye 대한척추신경외과학회 2024 Neurospine Vol.21 No.1

Objective: This study aimed to evaluate the effects of 2 endoscopic spine surgeries on the biomechanical properties of normal and osteoporotic spines. Methods: Based on computed tomography images of a healthy adult volunteer, 6 finite element models were created. After validating the normal intact model, a concentrated force of 400 N and a moment of 7.5 Nm were exerted on the upper surface of L3 to simulate 6 physiological activities of the spine. Five types of indices were used to assess the biomechanical properties of the 6 models, range of motion (ROM), maximum displacement value, intervertebral disc stress, maximum stress value, and articular protrusion stress, and by combining them with finite element stress cloud. Results: In normal and osteoporotic spines, there was no meaningful change in ROM or disc stress in the 2 surgical models for the 6 motion states. Model N1 (osteoporotic percutaneous transforaminal endoscopic discectomy model) showed a decrease in maximum displacement value of 20.28% in right lateral bending. Model M2 (unilateral biportal endoscopic model) increased maximum displacement values of 16.88% and 17.82% during left and right lateral bending, respectively. The maximum stress value of L4–5 increased by 11.72% for model M2 during left rotation. In addition, using the same surgical approach, ROM, maximum displacement values, disc stress, and maximum stress values were more significant in the osteoporotic model than in the normal model. Conclusion: In both normal and osteoporotic spines, both surgical approaches were less disruptive to the physiologic structure of the spine. Furthermore, using the same endoscopic spine surgery, normal spine biomechanical properties are superior to osteoporotic spines.

Bridge widening with composite steel-concrete girders: application and analysis of live load distribution

Yang, Yue,Zhang, Xiaoguang,Fan, Jiansheng,Bai, Yu Techno-Press 2015 Advances in concrete construction Vol.3 No.4

A bridge widening technology using steel-concrete composite system was developed and is presented in this paper. The widened superstructure system consists of a newly built composite steel-concrete girder with concrete deck and steel diaphragms attached to the existing concrete girders. This method has been applied in several bridge widening projects in China, and one of those projects is presented in detail. Due to the higher stiffness-to-weight ratio and the rapid erection of composite girders, this widening method reveals benefits in both mechanical performance and construction. As only a few methods for the design of bridges with different types of girders are recommended in current design codes, a more accurate analytical method of estimating live load distribution on girder bridges was developed. In the analytical model, the effects of span length, girder pacing, diaphragms, concrete decks were considered, as well as the torsional and flexural stiffness of both composite box girders and concrete T girders. The study shows that the AASHTO LRFD specification procedures and the analytical models proposed in this paper closely approximate the live load distribution factors determined by finite element analysis. A parametric study was also conducted using the finite element method to evaluate the potential load carrying capacities of the existing concrete girders after widening.

GC–MS analysis of chemical constituents and determination of the total antioxidant capacity of adult powder of Periplaneta americana

Yang Zhen,Xie Jiqin,Huang Feiyun,Yang Yu,Zhang Xiuyue,Yue Bisong 한국곤충학회 2022 Entomological Research Vol.52 No.2

Edible insects are alternative sources of high protein. Southeast Asia, South America, Africa, and Europe have recorded edible insects and, especially in Europe and America, there are factories that produce insects as food. In some areas of China, there has also been the habit of eating insects since ancient times. Periplaneta americana is an insect with the homology of medicine and food. In recent years, its medicinal and nutritional functions have attracted extensive attention and research. Its adult powder has been certified as a health product. It not only contains a variety of proteins but also is rich in fatty acids. The composition and antioxidant function of adult powder extract was analyzed in this study. Using adult P. americana powder as the raw material, it was extracted with n-Hexane, dichloromethane, and ethyl acetate, respectively. Gas chromatography–mass spectrometry (GC–MS) removed a total of 60 compounds, and many active components were extracted from P. americana powder for the first time. The parts and relative content of each extracted sample were obtained, and the total antioxidant capacity (T-AOC) of each extracted piece was determined, among which the antioxidant activity of ethyl acetate was the highest.

The Expression of Carnosine and Its Effect on the Antioxidant Capacity of Longissimus dorsi Muscle in Finishing Pigs Exposed to Constant Heat Stress

Yang, Peige,Hao, Yue,Feng, Jinghai,Lin, Hai,Feng, Yuejin,Wu, Xin,Yang, Xin,Gu, Xianhong Asian Australasian Association of Animal Productio 2014 Animal Bioscience Vol.27 No.12

The objective of this study was to assess the effects of constant high ambient temperatures on meat quality, antioxidant capacity, and carnosine expression in longissimus dorsi muscle of finishing pigs. Castrated 24 male DLY (crossbreeds between Landrace${\times}$Yorkshire sows and Duroc boars) pigs were allocated to one of three treatments: constant ambient temperature at $22^{\circ}C$ and ad libitum feeding (CON, n = 8); constant high ambient temperature at $30^{\circ}C$ and ad libitum feeding (H30, n = 8); and constant ambient temperature at $22^{\circ}C$ and pair-fed with H30 (PF, n = 8). Meat quality, malondialdehyde (MDA) content, antioxidant capacity, carnosine content, and carnosine synthetase (CARNS1) mRNA expression in longissimus dorsi muscle were measured after three weeks. The results revealed that H30 had lower $pH_{24h}$, redness at 45 min, and yellowness at 24 h post-mortem (p<0.05), and higher drip loss at 48 h and lightness at 24 h post-mortem (p<0.01). Constant heat stress disrupted the pro-oxidant/antioxidant balance in longissimus dorsi muscle with higher MDA content (p<0.01) and lower antioxidant capacity (p<0.01). Carnosine content and CARNS1 mRNA expression in longissimus dorsi muscle of H30 pigs were significantly decreased (p<0.01) after three weeks at $30^{\circ}C$. In conclusion, constant high ambient temperatures affect meat quality and antioxidant capacity negatively, and the reduction of muscle carnosine content is one of the probable reasons.

Siderophore-producing rhizobacteria reduce heavy metal-induced oxidative stress in Panax ginseng Meyer

Yue Huo,Jong Pyo Kang,Jong Chan Ahn,Yeon Ju Kim,Chun Hong Piao,Dong Uk Yang,Deok Chun Yang 고려인삼학회 2021 Journal of Ginseng Research Vol.45 No.2

Background: Panax ginseng is one of the most important medicinal plants and is usually harvested after 5 to 6 years of cultivation in Korea. Heavy metal (HM) exposure is a type of abiotic stress that can induce oxidative stress and decrease the quality of the ginseng crop. Siderophore-producing rhizobacteria (SPR) may be capable of bioremediating HM contamination. Methods: Several isolates from ginseng rhizosphere were evaluated by in vitro screening of their plant growth-promoting traits and HM resistance. Subsequently, in planta (pot tests) and in vitro (medium tests) were designed to investigate the SPR ability to reduce oxidative stress and enhance HM resistance in P. ginseng inoculated with the SPR candidate. Results: In vitro tests revealed that the siderophore-producing Mesorhizobium panacihumi DCY119<SUP>T</SUP> had higher HM resistance than the other tested isolates and was selected as the SPR candidate. In the planta experiments, 2-year-old ginseng seedlings exposed to 25 mL (500 mM) Fe solution had lower biomass and higher reactive oxygen species level than control seedlings. In contrast, seedlings treated with 10<SUP>8</SUP> CFU/mL DCY119<SUP>T</SUP> for 10 minutes had higher biomass and higher levels of antioxidant genes and nonenzymatic antioxidant chemicals than untreated seedlings. When Fe concentration in the medium was increased, DCY119<SUP>T</SUP> can produce siderophores and scavenge reactive oxygen species to reduce Fe toxicity in addition to providing indole-3-acetic acid to promote seedling growth, thereby conferring inoculated ginseng with HM resistance. Conclusions: It was confirmed that SPR DCY119<SUP>T</SUP> can potentially be used for bioremediation of HM contamination.