The complete chloroplast genome of two Syzygium (Myrtaceae) species and comparative analysis with other related species

Zhang Mengjie,Yang Xiuyao,Zhu Ling,Xi Dengxian,Cai Hanbing,Yin Tuo,Zhang Hanyao 한국식물생명공학회 2023 Plant biotechnology reports Vol.17 No.5

Syzygium, a vital genus under Myrtaceae, is aesthetically pleasing and resistant and is used for urban greenery. However, with intensive research in recent years, an increasing number of Syzygium plants have been explored for their medicinal value. Analysing the Syzygium chloroplast (cp) genome can help assess its phylogenetic relationships and provide a theoretical basis for the development and application of Syzygium plants. In this study, we first sequenced the complete cp genomes of Syzygium grijsii and Syzygium rehderianum to investigate their phylogenetic relationships in Myrtaceae. In the copy region and reverse repeat region, 129 genes were detected, including 84 protein-coding genes, 37 tRNA genes, and eight rRNA genes. Preference analysis showed that the eight Syzygium species preferred codons ending in A/U and contained abundant repetitive sequences and SSR sites. The ycf1, rpoC2, matK and ndhB genes had the most RNA editable sites and more genes with Ka/Ks < 1, indicating that species evolution was mainly subject to purifying selection. Comparative analysis of the cp genome showed that the eight species boundaries of Syzygium were similar, but the variability of the LSC and SSC regions was higher than that of the IR region, with 280 sites with Pi > 0.015, showing a high level of nucleotide diversity. Phylogenetic analysis showed that Syzygium grijsii was closely related to Syzygium jambos, and S. rehderianum was close to Plinia aureana. The cp genome and phylogeny of S. grijsii and S. rehderianum provide a theoretical basis for Syzygium species identification and exploitation.

Critical effects of long non-coding RNA on fibrosis diseases

Yue Zhang,Gang Luo,Yi Zhang,Mengjie Zhang,Jian Zhou,Weiwu Gao,Xiuyun Xuan,Xia Yang,Di Yang,Zhiqiang Tian,Bing Ni,Jun Tang 생화학분자생물학회 2018 Experimental and molecular medicine Vol.50 No.-

The expression or dysfunction of long non-coding RNAs (lncRNAs) is closely related to various hereditary diseases, autoimmune diseases, metabolic diseases and tumors. LncRNAs were also recently recognized as functional regulators of fibrosis, which is a secondary process in many of these diseases and a primary pathology in fibrosis diseases. We review the latest findings on lncRNAs in fibrosis diseases of the liver, myocardium, kidney, lung and peritoneum. We also discuss the potential of diseaserelated lncRNAs as therapeutic targets for the clinical treatment of human fibrosis diseases.

Wrinkled rGO Sheets-Wrapped Carbon Fibers with High Tensile Strength and Excellent Electrochemical Stability as Anodes for Structural Li-Ion Battery

Huagen Li,Shubin Wang,Mengjie Feng,Jiping Yang,Boming Zhang 성균관대학교(자연과학캠퍼스) 성균나노과학기술원 2018 NANO Vol.13 No.08

Herein, we report a hierarchical structure formed by wrinkled reduced graphene oxide (rGO) sheets-wrapped carbon fiber via a facile and efficient electrostatic self-assembly method and subsequent annealing treatment. For this material, the Weibull scale parameter is 4.77 GPa. After 100 cycles, the rGO@CF retains 91% of its second charge capacity at 50mA· g -1, corresponding to a capacity fading of only 0.09% per cycle. Thus, this structural anode material exhibits enhanced capacity, high initial Coulombic efficiency and high tensile strength. Meanwhile, the carbon fiber and interweaved rGO sheets together form the whole conductive networks to provide multichannel highways for charge transfer (lithium-ion diffusion and electron transport) during discharge–charge processes, promising excellent electrochemical performance of this structural anode material.

Construction of rough and porous surface of hydrophobic PTFE powderembedded PVDF hollow fiber composite membrane for accelerated water mass transfer of membrane distillation

Wenying Shi,Tengfei Li,Mengjie Fan,Hongbin Li,Haixia Zhang,Xiaohong Qin 한국공업화학회 2022 Journal of Industrial and Engineering Chemistry Vol.108 No.-

The construction of rough and porous hydrophobic membrane surface is expected to overcome the obstaclesincluding low permeate water flux and membrane pore wetting which greatly restrict the developmentof membrane distillation (MD) technology. In this study, a rough and porouspolytetrafluoroethylene (PTFE) powder-embedded polyvinylidene fluoride (PVDF) hydrophobic coatinglayer was compounded on the outer surface of PVDF hollow fiber support membrane by the dilute solutioncoating-phase inversion method. PVDF hollow fiber support membrane was fabricated by the dry-jetwet-spinning technique. PTFE powder was directly incorporated in PVDF dilute coating solution andembedded in the porous PVDF coating layer after the nonsolvent induced phase separation (NIPS). Thevariations of membrane morphology, surface chemical compositions, hydrophobicity and wetting resistancewere investigated by scanning electron microscope (SEM), Attenuated total reflection-Fouriertransform infrared spectroscopy (ATR-FTIR), dynamic water contact angle (WCA) and liquid entry pressure(LEP) analysis. Membrane separation performance including desalination and ionic dyes removalproperties was evaluated by VMD experiment. Compared with PVDF hollow fiber support membrane,both the surface hydrophobicity and the water permeability of the PTFE powder-embedded PVDF hollowfiber composite membrane (HFC) had obvious improvement. The surface WCA and permeate water fluxincreased from 92.6 and 11.3 kg/m2.h for PVDF support membrane to 133.6 and 26.8 kg/m2.h for thePTFE powder-embedded HFC membrane meanwhile NaCl rejection can be maintained above 99.9%(3.5 wt% NaCl aqueous solution at 50 C and permeate pressure at 31.3 kPa). The separation performanceof HFC membrane can remain stable without obvious pore wetting during the continuous MD operationfor 36 h. Different from the desalination, porous hollow fiber membrane would adsorb two differentcharged dyes, Congo red (CR) and methylene blue (MB) to a certain extent, resulting in the decrease ofmembrane flux and the change of permeate water quality. Finally, the MD separation mechanism of inorganicsalt, anionic dye and cationic dye by PTFE powder-embedded HFC membrane was proposed.

Experimental study on solidification of uranium tailings by microbial grouting combined with electroosmosis

Deng Jinxiang,Li Mengjie,Tian Yakun,Wu Lingling,Hu Lin,Zhang Zhijun,Zheng Huaimiao 한국원자력학회 2023 Nuclear Engineering and Technology Vol.55 No.12

The present microbial reinforcement of rock and soil exhibits limitations, such as uneven reinforcement effectiveness and low calcium carbonate generation rate, resulting in limited solidification strength. This study introduces electroosmosis as a standard microbial grouting reinforcement technique and investigates its solidification effects on microbial-reinforced uranium tailings. The most effective electroosmosis effect on uranium tailings occurs under a potential gradient of 1.25 V/cm. The findings indicate that a weak electric field can effectively promote microbial growth and biological activity and accelerate bacterial metabolism. The largest calcium carbonate production occurred under the gradient of 0.5 V/cm, featuring a good crystal combination and the best cementation effect. Staged electroosmosis and electrode conversion efficiently drive the migration of anions and cations. Under electroosmosis, the cohesion of uranium tailings reinforced by microorganisms increased by 37.3% and 64.8% compared to those reinforced by common microorganisms and undisturbed uranium tailings, respectively. The internal friction angle is also improved, significantly enhancing the uniformity of reinforcement and a denser and stronger microscopic structure. This research demonstrates that MICP technology enhances the solidification effects and uniformity of uranium tailings, providing a novel approach to maintaining the safety and stability of uranium tailings dams

An ABA triblock copolymer strategy for intrinsically stretchable semiconductors

Peng, Rui,Pang, Bo,Hu, Daqing,Chen, Mengjie,Zhang, Guobing,Wang, Xianghua,Lu, Hongbo,Cho, Kilwon,Qiu, Longzhen The Royal Society of Chemistry 2015 Journal of Materials Chemistry C Vol.3 No.15

<▼1><P>A novel semiconductor–rubber–semiconductor triblock copolymer has been designed and prepared according to the principle of thermoplastic elastomers (TPEs). It behaves as a TPE and exhibits good electrical properties.</P></▼1><▼2><P>A novel semiconductor–rubber–semiconductor (P3HT–PMA–P3HT) triblock copolymer has been designed and prepared according to the principle of thermoplastic elastomers. It behaves as a thermoplastic elastomer with a Young's modulus (<I>E</I>) of 6 MPa for an elongation at break of 140% and exhibits good electrical properties with a carrier mobility of 9 × 10<SUP>−4</SUP> cm<SUP>2</SUP> V<SUP>−1</SUP> s<SUP>−1</SUP>. This novel semiconductor may play an important role in low-cost and large-area stretchable electronics.</P></▼2>

The miR-145-5p/CD36 pathway mediates PCB2-induced apoptosis in MCF-7 cells

Yuan Yuan,Caihua Xue,Qiang Wu,Mengjie Wang,Jiahua Liu,Longfei Zhang,Qianwen Xing,Jingyan Liang,Hua Wu,Zhi Chen 한국유전학회 2021 Genes & Genomics Vol.43 No.2

Background Procyanidin B2 (PCB2) can increase the levels of anti-infammatory and immune mediators. Objectives However, its molecular mechanism in human breast cancer remains unclear. This study aimed to investigate the antitumor efect of PCB2 on MCF-7 cells and to examine the underlying mechanism. Methods The fow cytometry and EdU incorporation assays were measured the PCB2-induced BMECs. The expression levels of infammatory factors and immune response genes were upregulated in MCF-7 cells, high-throughput sequencing was used to detect diferentially expressed genes in blank and PCB2-treated MCF-7 cells. Results The results showed that PCB2 induced the apoptosis of MCF-7 cells. CD36 profles were afected in MCF-7 cells. Additionally, prediction software identifed a miR-145-5p binding site in the CD36 sequence. Luciferase reporter assays and Western blot analysis were used to verify the regulatory relationships between the diferentially expressed miRNA miR145-5p and CD36. MiR-145-5p and its key target (CD36) constitute a potential miRNA-mRNA regulatory pair. Functional studies in MCF-7 cells revealed that CD36 promotes but miR-145-5p inhibits apoptosis. Conclusion Overall, these data suggest that miR-145-5p inhibits the enhancing efect of PCB2 on CD36 expression by binding CD36 and subsequently regulating apoptosis, the immune response and anti-infammatory pathways. These results provide theoretical and experimental support for the treatment of breast cancer.