http://chineseinput.net/에서 pinyin(병음)방식으로 중국어를 변환할 수 있습니다.
변환된 중국어를 복사하여 사용하시면 됩니다.
Hai-Zhong Yu,Ming-Hui Liu,Xue-Yang Wang,Xin Yang,Wan-Ling Wang,Lei Geng,Dong Yu,Xue-Lan Liu,Gui-Ying Liu,Jia-Ping Xu 한국응용곤충학회 2016 Journal of Asia-Pacific Entomology Vol.19 No.3
Chitin deacetylase (CDA) is an insect chitin degradation enzyme that catalyzes the deacetylation of chitin to form chitosan. In this study, combination of rapid-amplification of cDNA ends (RACE) technology with Cnaphalocrocis medinalis transcriptome database analysis revealed the presence of at least five C. medinalis CDAs (CmCDAs), which were CmCDA1, CmCDA2, CmCDA4, CmCDA5, and CmCDA6. The cDNA sequences of CmCDA1, CmCDA2, and CmCDA4 hadwhole open reading frame (ORF) for further analysis. Phylogenetic analysis indicated that insect CDAs could be categorized into five groups. CmCDAs' structural domain analysis revealed that all three CDAs contained the chitin deacetylase-like catalytic domain. CmCDA1 and CmCDA2 belong to Group I because they both contain the chitin-binding peritrophin-A domain (ChBD), low-density lipoprotein receptor class A domain (LDLa), and chitin deacetylase-like catalytic domain. CmCDA4 only contains ChBD and chitin deacetylase-like catalytic domain thus belongs to Group III. Tissue and developmental stage expression analysis showed that the expression levels of CmCDA1, CmCDA2, and CmCDA4 are significantly higher in the head than other tissues and also significantly higher in adults than in larvae. CmCDA5 had significantly higher expression in the integument than other tissues, suggesting potential roles in the process of degradation of chitin. In contrast, CmCDA5 showed relatively high expression in larvae. In conclusion, this study analyzed the cDNA sequences of three CDA genes and determined their expression and molecular characteristics, which provided a new sequence resource and improved the development of bio-pesticides and the biological pest control and contributed to management of this important agricultural pest.
Hai-Zhong Yu a,Yu-Ling Huang,Ning-Yan Li,Yan-Xin Xie,Cheng-Hua Zhou,Zhan-Jun Lu 한국응용곤충학회 2019 Journal of Asia-Pacific Entomology Vol.22 No.4
Cathepsins belong to a group of mammalian papain-like cysteine proteases that play an important role in the insect immune response. In the present study, we identified two cathepsin genes from the Diaphorina citri genome database, cathepsin-L (DcCath-L) and cathepsin-O (DcCath-O). DcCath-L encodes a DcCath-L protein consisting of 348 amino acid residues, and DcCath-O encodes a DcCath-O protein consisting of 329 amino acid residues. DcCaths contain two conserved domains, the Inhibitor_I29 and Pept_C1 domains. Phylogenetic tree analysis revealed that DcCath-L and DcCath-O were divided into two different groups: Cathepsin-L and Cathepsin-O. Reverse transcription quantitative polymerase chain reaction analysis showed that both DcCath-O and DCCath-L were highly expressed in the midgut, while lower expression was observed in other tissues. Developmental stage expression analysis suggested that DcCath-O was mainly expressed in third instar nymph and adult, and DcCath-L was highly expressed in first and fourth instar nymph. Following exposure to two different heat-killed bacteria (Escherichia coli and Staphylococcus aureus) and Candidatus Liberibacter asiaticus, the expression of DcCath-O and DcCath-L was significantly increased and showed differential expression patterns at different time points. In addition, silencing of DcCath-L obvious affected the gene expression of members of the Toll pathway, while knock down of DcCath-L has no significantly influence. Overall, these data provide valuable information for further functional studies of D. citri cathepsins.
Yang Hui-Hui,Jiang Hui-Ling,Tao Jia-Hao,Zhang Chen-Yu,Xiong Jian-Bing,Yang Jin-Tong,Liu Yu-Biao,Zhong Wen-Jing,Guan Xin-Xin,Duan Jia-Xi,Zhang Yan-Feng,Liu Shao-Kun,Jiang Jian-Xin,Zhou Yong,Guan Cha-Xi 생화학분자생물학회 2022 Experimental and molecular medicine Vol.54 No.-
Necroptosis is the major cause of death in alveolar epithelial cells (AECs) during acute lung injury (ALI). Here, we report a previously unrecognized mechanism for necroptosis. We found an accumulation of mitochondrial citrate (citratemt) in lipopolysaccharide (LPS)-treated AECs because of the downregulation of Idh3α and citrate carrier (CIC, also known as Slc25a1). shRNA- or inhibitor–mediated inhibition of Idh3α and Slc25a1 induced citratemt accumulation and necroptosis in vitro. Mice with AEC-specific Idh3α and Slc25a1 deficiency exhibited exacerbated lung injury and AEC necroptosis. Interestingly, the overexpression of Idh3α and Slc25a1 decreased citratemt levels and rescued AECs from necroptosis. Mechanistically, citratemt accumulation induced mitochondrial fission and excessive mitophagy in AECs. Furthermore, citratemt directly interacted with FUN14 domain-containing protein 1 (FUNDC1) and promoted the interaction of FUNDC1 with dynamin-related protein 1 (DRP1), leading to excessive mitophagy-mediated necroptosis and thereby initiating and promoting ALI. Importantly, necroptosis induced by citratemt accumulation was inhibited in FUNDC1-knockout AECs. We show that citratemt accumulation is a novel target for protection against ALI involving necroptosis.
Study on the Flavonoids of Lespedeza hedysaroides
Yu-qing Wang,Alamus,Qi-zhong Sun,Hong-xin Wu 한국초지조사료학회 2009 한국초지조사료학회 학술대회논문집 Vol.2009 No.08
Chemical investigation of the aerial parts of Lespedaza hedysaroides resulted in the isolation of 8 flavonoids:orientin (1), isoorientin (2), vitexin (3), isovitexin (4), Isomyricitrin (5), meletin-3-O-β-d-glucoside (6), luteolin-7-O-glucoside (7), 6-Xylopyranosylluteolin (8), The result showed t㏊t Lespedeza hedysaroides was rich in flavonoid glycosides, espically of C-glycosylflavones which have good Pharmacia and bioactive. So Lespedeza hedysaroides was concluded that it has vast prospect for exploitation and utilization.
An Analytical Solution for Voltage Stability Studies Incorporating Wind Power
Yu-Zhang Lin,Li-Bao Shi,Liang-Zhong Yao,Yi-Xin Ni,Shi-Yao Qin,Rui-Ming Wang,Jin-Ping Zhang 대한전기학회 2015 Journal of Electrical Engineering & Technology Vol.10 No.3
Voltage stability is one of the most critical security issues which has not yet been well resolved to date. In this paper, an analytical method called PQ plane analysis with consideration of the reactive power capability of wind turbine generator and the wake effect of wind farm is proposed for voltage stability study. Two voltage stability indices based on the proposed PQ plane analysis method incorporating the uncertainties of load-increasing direction and wind generation are designed and implemented. Cases studies are conducted to investigate the impacts of wind power incorporation with different control modes. Simulation results demonstrate that the constant voltage control based on reactive power capability significantly enhances voltage stability in comparison of the conventional constant power factor control. Some meaningful conclusions are obtained.
Xin Sun,Tao Zhang,Qifei Deng,Qirui Zhou,Xianchao Sun,Enlai Li,Dexin Yu,Caiyun Zhong 한국분자세포생물학회 2018 Molecules and cells Vol.41 No.3
Benzidine, a known carcinogen, is closely associated with the development of bladder cancer (BC). Epithelial–mesenchymal transition (EMT) is a critical pathophysiological process in BC progression. The underlying molecular mechanisms of mitogen-activated protein kinase (MAPK) pathway, especially extracellular regulated protein kinases 5 (ERK5), in regulating benzidine-induced EMT remains unclarified. Hence, two human bladder cell lines, T24 and EJ, were utilized in our study. Briefly, cell migration was assessed by wound healing assay, and cell invasion was determined by Transwell assay. Quantitative PCR and western blot were utilized to determine both gene expressions as well as protein levels of EMT and MAPK, respectively. Small interfering RNA (siRNA) was transfected to further determine ERK5 function. As a result, the migration and invasion abilities were enhanced, epithelial marker ex-pression was decreased while mesenchymal marker expression was increased in human BC cell lines. Meanwhile, benzidine administration led to activation of ERK5 and activator protein 1 (AP-1) proteins, without effective stimulation of the Jun N-terminal kinase (JNK) or p38 pathways. Moreover, Benzidine-induced EMT and ERK5 activation were completely suppressed by XMD8-92 and siRNAs specific to ERK5. Of note, ERK1/2 was acti-vated in benzidine-treated T24 cells, while benzidine-induced EMT could not be reversed by U0126, an ERK1/2 inhibitor, as indicated by further study. Collectively, our findings revealed that ERK5-mediated EMT was critically involved in benzidine-correlated BC progression, indicating the therapeutic significance of ERK5 in benzidine-related BC.
Experimental study on the effect of EC-TMD on the vibration control of plant structure of PSPPs
Xin Feng,Tengfei Zhong,Yu Zhang,Jing Zhou 국제구조공학회 2022 Smart Structures and Systems, An International Jou Vol.29 No.3
A high-frequency vibration control method is proposed in this paper for Pumped Storage Power Plants (PSPPs) using Eddy Current Tuned Mass Damper (EC-TMD), based on which a new type of EC-TMD device is designed. The eddy current damper parameters are optimized by numerical simulation. On this basis, physical simulation model tests are conducted to compare and study the effect of structural performance with and without damping, different control strategies, and different arrangement positions of TMD. The test results show that EC-TMD can effectively reduce the control effect under high-frequency vibration of the plant structure, and after the additional damping device forms EC-TMD, the energy dissipation is further realized due to the intervention of eddy current damping, and the control effect is subsequently improved. The Multi-Tuned Mass Damper (MTMD) control strategy broadens the tuning band to improve the robustness of the system, and the vibration advantage is more obvious. Also, some suggestions are made for the placement of the dampers to promote their application.
( Xin Liu ),( Ying Dong ),( Jingquan Wang ),( Long Li ),( Zhenmin-zhong ),( Yun-pan Li ),( Shao-jun Chen ),( Yu-cai Fu ),( Wen-can Xu ),( Chi-ju Wei ) 한국미생물생명공학회(구 한국산업미생물학회) 2017 Journal of microbiology and biotechnology Vol.27 No.6
Vesicular stomatitis virus G glycoprotein (VSV-G) has been widely used for pseudotyping retroviral, lentiviral, and artificial viral vectors. The objective of this study was to establish a potential approach for large-scale production of VSV-G. To this end, VSV-G was cloned with an N-terminal His-tag into Pichia pastoris expression vector pPIC3.5K. Three clones (Mut<sup>s</sup>) containing the VSV-G expression cassette were identified by PCR. All clones proliferated normally in expansion medium, whereas the proliferation was reduced significantly under induction conditions. VSV-G protein was detected in cell lysates by western blot analysis, and the highest expression level was observed at 96 h post induction. VSV-G could also be obtained from the condition medium of yeast protoplasts. Furthermore, VSV-G could be incorporated into Ad293 cells and was able to induce cell fusion, leading to the transfer of cytoplasmic protein. Finally, VSV-G-mediated DNA transfection was assayed by flow cytometry and luciferase measurement. Incubation of VSV-G lysate with the pGL3-control DNA complex increased the luciferase activity in Ad293 and HeLa cells by about 3-fold. Likewise, incubation of VSV-G lysate with the pCMV-DsRed DNA complex improved the transfection efficiency into Ad293 by 10% and into HeLa cells by about 1-fold. In conclusion, these results demonstrate that VSV-G could be produced from P. pastoris with biofunctionalities, demonstrating that large-scale production of the viral glycoprotein is feasible.