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( Chen Chen ),( Ming Zhong Sun ),( Shu Qing Liu ),( Dong Mei Yeh ),( Li Jun Yu ),( Yang Song ),( Lin Lin Gong ),( Li Hong Hao ),( Jun Hu ),( Shu Juan Shao ) 생화학분자생물학회 (구 한국생화학분자생물학회) 2010 BMB Reports Vol.43 No.8
Smad4 is involved in cancer progression and metastasis. Using a pair of human syngeneic epithelial ovarian cancer cells with low (HO-8910) and high (HO-8910PM) metastatic abilities, we aimed to reveal the role of Smad4 in ovarian cancer metastasis in vitro. Smad4 was down-regulated in HO-8910PM cell line relative to HO-8910 by implicating Smad4 was probably a potential tumor suppressor gene for ovarian cancer. Re-expression of Smad4 decreased the migration ability and inhibited the invasion capacity of HO-8910PM, while promoted the cell adhesion capacity for HO-8910PM. The stable expression of Smad4 increased the expression of E-cadherin, reduced the expression of plasminogen activator inhibitor-1 (PAI-1) and slightly down-regulated the expression of VEGF. Smad4 suppresses human ovarian cancer cell metastasis potential through its effect on the expressions of PAI-1, E-cadherin and VEGF. Results from current work implicate Smad4 might suppress the invasion and metastasis of human ovarian tumor cells through a TGF-β/Smad-mediated pathway. [BMB reports 2010; 43(8): 554-560]
Chen, Jun-Xing,Deng, Nan,Chen, Xu,Chen, Ling-Wu,Qiu, Shao-Peng,Li, Xiao-Fei,Li, Jia-Ping Asian Pacific Journal of Cancer Prevention 2012 Asian Pacific journal of cancer prevention Vol.13 No.5
Purpose: To assess efficacy of Ki67 combined with VEGF as a molecular grading model to predict outcomes with non-muscle invasive bladder cancer (NMIBC). Materials: 72 NMIBC patients who underwent transurethral resection (TUR) followed by routine intravesical instillations were retrospectively analyzed in this study. Univariate and multivariate analyses were performed to confirm the prognostic values of the Ki67 labeling index (LI) and VEGF scoring for tumor recurrence and progression. Results: The novel molecular grading model for NMIBC contained three molecular grades including mG1 (Ki67 $LI{\leq}25%$, VEGF $scoring{\leq}8$), mG2 (Ki67 LI>25%, VEGF $scoring{\leq}8$; or Ki67 $LI{\leq}25%$, VEGF scoring > 8), and mG3 (Ki67 LI > 25%, VEGF scoring > 8), which can indicate favorable, intermediate and poor prognosis, respectively. Conclusions: The described novel molecular grading model utilizing Ki67 LI and VEGF scoring is helpful to effectively and accurately predict outcomes and optimize personal therapy.
Identification of a Potential Anticancer Target of Danshensu by Inverse Docking
Chen, Shao-Jun,Ren, Ji-Long Asian Pacific Journal of Cancer Prevention 2014 Asian Pacific journal of cancer prevention Vol.15 No.1
Objective: To study potential targets of Danshensu via dual inverse docking. Method: PharmMapper and idTarget servers were used as tools, and the results were checked with the molecular docking program autodock vina in PyRx 0.8. Result: The disease-related target HRas was rated top, with a pharmacophore model matching well the molecular features of Danshensu. In addition, docking results indicated that the complex was also matched in terms of structure, H-bonds, and hydrophobicity. Conclusion: Dual inverse docking indicates that HRas may be a potential anticancer target of Danshensu. This approach can provide useful information for studying pharmacological effects of agents of interest.
Chen, Shao-Jun Asian Pacific Journal of Cancer Prevention 2014 Asian Pacific journal of cancer prevention Vol.15 No.10
Tanshinone IIA is a pharmacologically active ingredient extracted from Danshen, a Chinese traditional medicine. Its molecular mechanisms are still unclear. The present study utilized computational approaches to uncover the potential targets of this compound. In this research, PharmMapper server was used as the inverse docking tool andnd the results were verified by Autodock vina in PyRx 0.8, and by DRAR-CPI, a server for drug repositioning via the chemical-protein interactome. Results showed that the retinoic acid receptor alpha ($RAR{\alpha}$), a target protein in acute promyelocytic leukemia (APL), was in the top rank, with a pharmacophore model matching well the molecular features of Tanshinone IIA. Moreover, molecular docking and drug repurposing results showed that the complex was also matched in terms of structure and chemical-protein interactions. These results indicated that $RAR{\alpha}$ may be a potential target of Tanshinone IIA for APL. The study can provide useful information for further biological and biochemical research on natural compounds.
Ya-Jun Gong,Jin-Cui Chen,Shao-Kun Guo,Pan Shi,Li-Jun Cao,Ming-Liang Li,Ary A. Hoffmann,Shu-Jun Wei 한국응용곤충학회 2020 Journal of Asia-Pacific Entomology Vol.23 No.4
The fall webworm (FWW) Hyphantria cunea, native to North America, is a globally invasive pest of a wide range of forest and fruit trees. Spraying of pesticides is the primary method for the control of FWW. In this study, toxicity and feeding cessation of two potential pesticides against the FWW, chlorantraniliprole, and chromafenozide, were evaluated. Both pesticides were slow to affect FWW. For chlorantraniliprole, the highest mortality of third instar larvae occurred at 72 h with an LC 50 of 10.34 mg/L, while for chromafenozide, the highest mortality occurred at 72 h with an LC 50 value 74.0950 mg/L. Low concentrations of both pesticides led to larvae ceasing to feed after six hours (chlorantraniliprole) and 24 h (chromafenozide). Both pesticides had persistent effects; thirty days after being applied at concentrations of 16, 26.67, and 35.56 mg/L to leaves, 93.33% of newly contacted larvae died after seven days. Our study showed that chlorantraniliprole and chromafenozide could be alternatively used against FWW and form a component of integrated control programs. The results provide information to guide the usage of chlorantraniliprole and chromafenozide in FWW control.
Mycotoxins Containing Diet Affects Oocyte Quality in Mouse
Shao-Chen Sun,Yan-Jun Hou,Xiang-Shun Cui,Nam-Hyung Kim 한국동물생명공학회(구 한국동물번식학회) 2013 Reproductive & Developmental Biology(Supplement) Vol.37 No.2s
Background: Mycotoxins which mainly consist of Aflatoxin (AF), Zearalenone (ZEN) and Deoxynivalenol (DON) are commonly found in many food commodities, each component has been shown to cause organ toxicity and oxidative stress in several species. Our previous study showed that mycotoxin-contaminated diet could cause oxidative stress in liver, kidney, spleen. Recently we examined its effects on oocyte quality. Materials and Methods: Mycotoxins-contaminated maize (AF 597μg/kg, ZEN 729μg/kg, DON 3.1mg/kg maize) was incorporated into the diet at three different doses (0, 5 and 20%) to feed the mice for 4 weeks. Results: Our results showed that the both the index of ovary and the number of good GV oocytes decreased in the mycotoxin-treated mice. The oocytes from mycotoxin- treated mouse displayed low developmental competence showing with lower GVBD and polar body extrusion rate; the embryo developmental competence also showed the similar pattern, most embryos could not develop to blastocyst stage. The cytoskeleton component actin expression in both oocyte cortex and cytoplasm decreased, and the expression of actin nucleation factor Profilin and mDia1 also decreased, indicating that mycotoxin may affect oocyte quality through the effects on actin. Moreover, a big proportion of oocytes with mycotoxin contaminated diet treatment showed disrupted cortical granule free domain, spindle morphology and mitochondria distribution, further confirmed the oocyte quality declination. We also used the in vitro model to confirm this, we cultured the oocytes in the medium with Zearalenone, a key component of mycotoxins, and the results were similar with the in vivo model. Conclusion: Our data indicated that the mycotoxins were toxic to mouse reproductive system and induced the oocyte quality declination.
Adaptive Prescribed-time Control for Coupled 6-DOF Spacecraft Formation Flying
Li Chen,Yongyan Sun,Xiaowei Shao,Junli Chen,Dexin Zhang 제어·로봇·시스템학회 2023 International Journal of Control, Automation, and Vol.21 No.2
This paper studies the prescribed-time spacecraft formation flying problem under known and unknown external disturbance. The coupled 6-degrees-of-freedom relative kinematics and dynamics for spacecraft are modeled by using exponential coordinates of SE(3). With the help of the linear time-varying Lyapunov inequality based prescribed-time stability theorem, a novel global adaptive prescribed-time time-varying high-gain control law is designed by backstepping. It is shown that the designed controller is bounded. Numerical simulations verify the effectiveness of the proposed control method.
Yuan, Shao-Fei,Chen, Wen-Jun,Zhu, Lin-Jia,Zheng, Wei-E.,Chen, Hua,Xiong, Jian-Ping Asian Pacific Journal of Cancer Prevention 2012 Asian Pacific journal of cancer prevention Vol.13 No.6
Objective: To explore whether monoclonal antibodies against stathmin and the chemotherapuetic agent paclitaxel have synergenic effects in inhibiting growth and inducing apoptosis in human QG-56 cells. Methods: QG-56 cells were treated with monoclonal antibodies against stathmin or paclitaxel alone or in combination, with untreated cells used as controls. After 24, 48, 72 and 96 hours the cell growth condition was observed under an inverted microscope and inhibition was studied by MTT assay; apoptosis was analyzed by flow cytometry. Results: The populations decreased and cell shape and size changed after the various treatments. Monoclonal antibodies against stathmin and paclitaxel used alone or incombination inhibited the proliferation of QG-56 cells, especially in combination with synergism (P<0.05). Combined treatment also resulted in a significantly higher apoptosis rate than in the other groups (P<0.05). Conclusions: Monoclonal antibodies against stathmin and paclitaxel used alone or in combination can inhibit proliferation of QG-56 cells and induce apoptosis when applied together. The observed synergistic effects may have important implications for clinical application.
Phase II Clinical Study on the GEMOX Regimen as Second-line Therapy for Advanced Ovarian Cancer
Yuan, Shao-Fei,Zhang, Lian-Ping,Zhu, Lin-Jia,Chen, Wen-Jun,Zheng, Wei-E,Xiong, Jian-Ping Asian Pacific Journal of Cancer Prevention 2013 Asian Pacific journal of cancer prevention Vol.14 No.6
Aim: To investigate the effectiveness and adverse effects of gemcitabine by fixed-dose rate infusion plus oxaliplatin (GEMOX regimen) as second-line therapy for advanced ovarian cancer. Methods: 64 patients with advanced ovarian cancer were divided into an experimental group (44 cases) and a control group (20 cases). The experimental group was treated with continuous intravenous infusion of gemcitabine at 1000 $mg/m^2$ with a fixed-dose rate of 10 $mg/m^2/min$, on days 1 and 8 and oxaliplatin at 100 $mg/m^2$ on day 1, IVGTT, repeated every 3 weeks. The control group was treated with intravenous infusion of gemcitabine at 1000 $mg/m^2$ within 30 min on days 1 and and oxaliplatin at 100 $mg/m^2$ on day 1, IVGTT, again repeated every 3 weeks. CT scans or MRI were used for review every 1-2 cycles. Results: The effective rate in the experimental group was significantly high than control group (43.2% vs 35.0%; P < 0.05), with no obvious difference of hematologic or non-hematologic toxicity between the two groups (P > 0.05). Conclusion: GEMOX regimen is very effective to treat advanced ovarian cancer, with low toxicity, good tolerance and improved life quality in patients.