Silencing downstream of receptor kinase gene (drk) impairs larval-pupal ecdysis in Leptinotarsa decemlineata (Say)

Pan Deng,Jun-Li Du,Li-Li Mu,Kai-Yun Fu,Wen-Chao Guo,Guo-Qing Li 한국응용곤충학회 2020 Journal of Asia-Pacific Entomology Vol.23 No.1

In insects, an insulin-like peptide (ILP) triggers the formation of the insulin receptor (InR)/the insulin receptor substrate Chico complex. The complex then recruits downstream of receptor kinase (Drk) and phosphatidylinositol-3-kinase (PI3K) to initiate two signaling branches, i.e., Drk-mitogen-activated protein kinase (MAPK) and Pi3K-protein kinase B subdivisions. Previous findings reveal that RNA interference (RNAi) of LdILP2 or Ldchico, rather than Ldpi3k92E, impairs larval-pupal and pupal-adult molting in the Colorado potato beetle Leptinotarsa decemlineata. It is accordingly hypothesized that the Drk-MAPK branch regulates larval metamorphosis. In the present paper, we first found that silencing LdILP2, Ldchico or Ldpi3k92E did not decrease the expression level of Lddrk, indicating other receptor tyrosine kinases’ signaling except insulin pathway is not affected in the RNAi larvae. Moreover, two InRs and Torso were highly expressed in the final larval instars. Furthermore, RNAi of either Lddrk or Ldchico, or both of them equally affected larval-pupal and pupal-adult molts, and similarly repressed the expression of representative MAPK (Ldras and Ldraf), ecdysteroidogenesis (Ldphm and Ldsad), and 20E signaling (LdEcR, LdUSP, LdHR3 and LdE75) genes. 20E feeding by Lddrk RNAi larvae completely restored the reduced mRNA levels of LdEcR, LdHR3 and LdE75, but did not rescued the decreased Lddrk and LdUSP levels and the lowered pupation and emergence rates. Therefore, our findings suggest that the Drk-MAPK branch is involved in metamorphosis regulation in L. decemlineata.

외국인 대학원 유학생의 학습 과정 대한 셀프 내러티브

리윈윈(Yun-Yun Li),푸신지(Xin-Ji Fu),천판판(Pan-Pan Chen) 한국교육과정개발원 2023 한국교육과정개발연구 Vol.2 No.1

셀프 내러티브 탐구를 통하여 더 많은 유학생들이 한국 생활에 적응할 수 있도록 도움을 주고자 하는 것이 본 연구의 목적이다. 언어와 문화적 어려움, 글의 작성에 대한 어려움을 말하였지만 이러한 어려움들을 잘 극복하는 일은 내가 누구인가, 나는 왜 학습을 하는가, 공부의 목적을 어디에 둘 것인가 하는 문제 등을 지속적으로 성찰하다 보면 해결은 잘 될 것으로 본다.더 많은 유학생들이 한국에 와서 꿈과 자존감을 실현할 수 있도록 인사이트를 제공할 수 있기를 바란다. The purpose of this study is to help more international students adapt to Korean life through self-narrative exploration. Language and cultural difficulties, and difficulties in writing, but I think the solution will be successful if I continue to reflect on the problems of who I am, why I learn, and where to put the purpose of studying. It is hoped that more international students can come to Korea and provide insights to realize their dreams and self-esteem.

Study of Differentiation and Characteristics of Sieve Elements and P-Protein Body in Gymnosperm Ginkgo

Yu Ping Ling,Peng Chen,Li Li,Li Li Wang,Bin Pan,Yun Lin Tai 한국원예학회 2006 한국원예학회 기타간행물 Vol.- No.-

High-j Proton h11/2 and g7/2 Intruder Bands in 113In

Ma Ke Yan,Lu Jing Bin,Ma Ying Jun,Li Jian,Yang Dong,Sun Wu Ji,Wang Hao,Pan Hao Nan,Wang Jia Qi,Yang Qing Yu,Zhang Da Ming,Zhu Li Hua,Wu Xiao Guang,Zheng Yun,Li Cong Bo 한국물리학회 2020 THE JOURNAL OF THE KOREAN PHYSICAL SOCIETY Vol.76 No.12

Excited states of 113In have been populated through the heavy-ion fusion evaporation reaction 110Pd(7Li, 4n)113In. A new band with the configuration of a proton d5/2 orbital is identified. Two ΔI = 2 intruder bands, built on the πh11/2 and the πg7/2 orbitals, have been extended to spins (63/2-)ħ and (55/2+)ħ, respectively. The negative-parity πh11/2 intruder band shows a smooth increase in aligned spin, which is attributed to a strong proton-neutron interaction. The properties of the positive-parity πg7/2 band are discussed based on tilted axis cranking model calculations, and the features of the antimagnetic rotation for this band are shown after backbend. Furthermore, the contributions of the two-shears-like mechanism, the neutron (gd)ν shell and the core rotation are investigated for the positive-parity πg7/2 band.

The Fingerprinting of Huangjinju Powder for Injection on Chinese Patent Medicine by XRD Fourier

Pan, Yan-Li,Zhang, Gui-Jun,Gong, Ning-Bo,Wu, Yun-Shan,Lu, Yang,Luo, Rong,Choi, Ho-Young The Korean Society of Medicinal Crop Science 2006 한국약용작물학회지 Vol.14 No.2

The purpose is to study the identification method of Huangjinju powder for injection and the medicinal materials by the fingerprint off-ray Diffraction Fourier (XRDF). We used the same method on both the studying of Huangjinju and the medicinal materials. Then we selected a few components alignment to compare. We analyzed the data by setting up the deviation $d({\AA})$ as ${\pm}0.05$ to calculate the rate of special mark on the sample (Px) and on the patent (P). The special XRDF of Huangjinju$[d({\AA})/(I/I_0)]$ have 5 peaks that have not expressed in medicinal materials. Therefore Px is 22.73%. Flos Trollii Chinensis has 3 special marks and Px is 17.65%. Flos Chrysanthemi Indici has 1 special mark and Px is 3.57%. Its coincided interplanar spacing with the patent is $2.907{\AA}$. Flos Lonicerae Japonicae has 6 special marks and Px is 23.08%. Its special mark in the patent are 4.95/14 and 4.50/15, respectively. The P is 9.09%. Its coincided interplanar spacing with the patent is $2.910{\AA}\;and\;3.05{\AA}$, respectively. The number of special XRDF mark peaks of baicalin is 9 and Px is 18.37%. Its coincided interplanar spacing with the patent is $2.910{\AA}$. It has visible mark and specificity adopting XRDF fingerprint to identify Huangjinju and medicinal materials. Establishing the quality standard is a synthetic index that depends both on special marks in the medicinal materials of the patent and on the coincidence peak data.

The Fingerprinting of Huangjinju Powder for Injection on Chinese Patent Medicine by XRD Fourier

Yan-Li Pan,Gui-Jun Zhang,Ning-Bo Gong,Yun-Shan Wu,Yang Lu,Rong Luo,Ho-Young Choi 韓國藥用作物學會 2006 한국약용작물학회지 Vol.14 No.2

The purpose is to study the identification method of Huangjinju powder for injection and the medicinal materials by the fingerprint off-ray Diffraction Fourier (XRDF). We used the same method on both the studying of Huangjinju and the medicinal materials. Then we selected a few components alignment to compare. We analyzed the data by setting up the deviation d(a) as ±0.05 to calculate the rate of special mark on the sample (Px) and on the patent (P). The special XRDF of Huangjinju[d(a)/(I/I0)] have 5 peaks that have not expressed in medicinal materials. Therefore Px is 22.73%. Flos Trollii Chinensis has 3 special marks and Px is 17.65%. Flos Chrysanthemi Indici has 1 special mark and Px is 3.57%. Its coincided interplanar spacing with the patent is 2.907a. Flos Lonicerae Japonicae has 6 special marks and Px is 23.08%. Its special mark in the patent are 4.95/14 and 4.50/15, respectively. The P is 9.09%. Its coincided interplanar spacing with the patent is 2.910a and 3.05a, respectively. The number of special XRDF mark peaks of baicalin is 9 and Px is 18.37%. Its coincided interplanar spacing with the patent is 2.910a. It has visible mark and specificity adopting XRDF fingerprint to identify Huangjinju and medicinal materials. Establishing the quality standard is a synthetic index that depends both on special marks in the medicinal materials of the patent and on the coincidence peak data.

Knowledge of Human Papillomavirus (HPV) Infection, Cervical Cancer, and HPV Vaccine and its Correlates among Medical Students in Southwest China: a Multi-center Cross-sectional Survey

Wen, Ying,Pan, Xiong-Fei,Zhao, Zhi-Mei,Chen, Feng,Fu, Chun-Jing,Li, Si-Qi,Zhao, Yun,Chang, Hong,Xue, Qing-Ping,Yang, Chun-Xia Asian Pacific Journal of Cancer Prevention 2014 Asian Pacific journal of cancer prevention Vol.15 No.14

Background: Since cervical cancer can be prevented and controlled through human papillomavirus (HPV) vaccination, it is important to train health care providers and provide them with appropriate knowledge. This study aimed to understand the level of HPV related knowledge among medical students and correlates in Southwest China in order to address any potential gap in their knowledge base. Methods: We conducted a cross-sectional survey among medical students in six selected universities across Southwest China based on a pretested questionnaire regarding HPV infection, cervical cancer, and HPV vaccines. Results: We successfully surveyed 1, 878 medical students, of whom 32.1% were males and 67.9% were females. Their mean age was 20.8 (standard deviation: 1.3) years. 91.8% of them were ethnic Han Chinese, and 43.8% were students in clinical medicine. While 76.5% had heard of HPV only 48.8% knew that the prevention of cervical cancer was possible through HPV vaccination. Only 42.9% of the male and 49.2% of the female students correctly answered over 10 out of 22 questions on HPV related knowledge. Female students appeared to know more about HPV and HPV vaccination (OR: 1.39; 95% CI: 1.11-1.75). In addition, the student knowledge improved with the grade (p<0.001). University courses were the most selected source of knowledge about HPV vaccination (66.4%). 83.6% of males and 91.4% of females were willing to have more HPV related education by experts (p<0.001). Only 10.1% of the students acknowledged that people had asked for their advice regarding HPV vaccination. Conclusions: Our survey indicates that medical students from Southwest China have poor knowledge of HPV and HPV vaccination, but are willing to receive more relevant information. Targeted education should be incorporated into school courses to inform these future health care providers and ensure success of programs for cervical cancer control and prevention.

Calcineurin-Responsive Transcription Factor CgCrzA Is Required for Cell Wall Integrity and Infection-Related Morphogenesis in Colletotrichum gloeosporioides

Ping Wang,Bing Li,Yu-Ting Pan,Yun-Zhao Zhang,De-Wei Li,Lin Huang 한국식물병리학회 2020 Plant Pathology Journal Vol.36 No.5

The ascomycete fungus Colletotrichum gloeosporioides infects a wide range of plant hosts and causes enormous economic losses in the world. The transcription factors (TFs) play an important role in development and pathogenicity of many organisms. In this study, we found that the C2H2 TF CgCrzA is localized in both cytoplasm and nucleus under standard condition, and it translocated from cytoplasm to nucleus in a calcineurin- dependent manner. Moreover, the ΔCgCrzA was hypersensitive to cell wall perturbing agents and showed severe cell wall integrity defects. Deletion of the CgCRZA inhibited the development of invasive structures and lost pathogenicity to plant hosts. Our results suggested that calcineurin-responsive TF CgCrzA was not only involved in regulating cell wall integrity, but also in morphogenesis and virulence in C. gloeosporioides.

Acetylation of Smc3 by Eco1 Is Required for S Phase Sister Chromatid Cohesion in Both Human and Yeast

Zhang, Jinglan,Shi, Xiaomin,Li, Yehua,Kim, Beom-Jun,Jia, Junling,Huang, Zhiwei,Yang, Tao,Fu, Xiaoyong,Jung, Sung Yun,Wang, Yi,Zhang, Pumin,Kim, Seong-Tae,Pan, Xuewen,Qin, Jun Elsevier 2008 Molecular cell Vol.31 No.1

<P><B>Summary</B></P><P>Sister chromatid cohesion is normally established in S phase in a process that depends on the cohesion establishment factor Eco1, a conserved acetyltransferase. However, due to the lack of known in vivo substrates, how Eco1 regulates cohesion is not understood. Here we report that yeast Eco1 and its human ortholog, ESCO1, both acetylate Smc3, a component of the cohesin complex that physically holds the sister chromatid together, at two conserved lysine residues. Mutating these lysine residues to a nonacetylatable form leads to increased loss of sister chromatid cohesion and genome instability in both yeast and human. In addition, we clarified that the acetyltransferase activity of Eco1 is essential for its function. Our study thus identified a molecular target for the acetyltransferase Eco1 and revealed that Smc3 acetylation is a conserved mechanism in regulating sister chromatid cohesion.</P>

Research on effect of rings on coupling vibration in a rigid-disk rotor

YiJui Chiu,Xiao-Yun Li,Guo-Fei Yu,Chia-Hao Yang,Jeng-Shyang Pan,ChinLing Chen 대한기계학회 2020 JOURNAL OF MECHANICAL SCIENCE AND TECHNOLOGY Vol.34 No.2

This paper uses new deductions of the equation of motion and obtains new modes of change that differ from the authors’ previous research. The blade-bending, shaft-torsion and ringbending coupled vibration influence of a single-disk rotor whose blade subsystem was coated with a ring is investigated. In the last two years, the authors adopted two methods, the assumed mode method and finite element method, and compared both parties results. This article continues that work. In this paper, the phenomena of ring joint coupling vibrations are different and interesting. The authors defined two new types, the shaft-blade-ring (SBR) and blade-ring (BR) modes. These two coupling modes can be mutually affected by the shaft, blade, and ring, and no single subsystem predominates. The new change rules of natural frequencies of rotors with two to 15 blades and those of mode shapes are discussed. The influence of the ring thickness and rotational speed are also studied in this paper.